Problem-solving is the cornerstone of all walks of scientific research. Fascinating Problems for Young Physicists attempts to clear the boundaries of seemingly abstract physical laws and their tangible effects through a step-by-step approach to physics in the world around us. It consists of 42 problems with detailed solutions, each describing a specific, interesting physical phenomenon. Each problem is further divided into questions designed to guide the reader through, encouraging engagement with and learning the physics behind the phenomenon. By solving the problems, the reader will be able to discover, for example, what the relation is between the mass of an animal and its expected lifetime, or what the efficiency limit is of wind turbines. Intended for first-year undergraduate students and interested high school students, this book develops inquiry-based scientific practice and enables students to acquire the necessary skills for applying the laws of physics to realistic situations.

Expanded and updated, The CRC Handbook of Laboratory Safety, Fifth Edition provides information on planning and building a facility, developing an organization infrastructure, planning for emergencies and contingencies, choosing the correct equipment, developing operational plans, and meeting regulatory requirements. Still the essential reference tool, the New Edition helps you organize your safety efforts to adhere to the latest regulations and use the newest technology.

Thoroughly revised, the CRC Handbook of Laboratory Safety, Fifth Edition includes new OSHA laboratory safety standards, the 1994 NRC radiation safety standards, guidelines for X-ray use in hospitals, enforcement of standards for dealing with blood-borne pathogens, OSHA actions covering hazardous waste operations and emergency response, and the latest CDC guidelines for research with microbial hazards. Every word on every page has been scrutinized, and literally hundreds of changes have been made to bring the material up to date.

See what's new in the New Edition
o New figures and tables illustrating the new material
o Internet references in addition to journal articles
o Changes in the Clean Air Act regarding incineration of hospital, medical, and infectious waste
o Obsolete articles removed and replaced - over one hundred pages of new material
o New information on respiratory protection guidelines

Drosophila is the scientist's favourite lab organism because it reproduces quickly and has straightforwartd chromosomes. It has long been the organism of choice in experiments in genetics and is also popular in developmental and behavioural studies. The second edition of this popular Practical Approach book brings the methodology up to date, with contributions from the world's leading Drosophila researchers. It will be valuable to the novice and experienced worker alike.

This is the bench and field scientist's guide to well-established, reliable techniques for use in microbiology and microbial ecology. It provides a good starting place for those who are beginning to investigate aspects of the microbial community, and a refresher for more experienced researchers. Chapters on bacteria with interesting metabolic traits are augmented with chapters on molecular techniques, lipis analysis, and appropriate sampling techniques. A special section includes valuable information on biofilm development, bioremediation, modeling of biological systems, and the study of phylogenetics. Unlike other texts, which present theory in microbial ecology, this one contains the applications that can be used throughout one's research.

This companion volume to "Fundamental Polymer Science" (Gedde and Hedenqvist, 2019) offers detailed insights from leading practitioners into experimental methods, simulation and modelling, mechanical and transport properties, processing, and sustainability issues. Separate chapters are devoted to thermal analysis, microscopy, spectroscopy, scattering methods, and chromatography. Special problems and pitfalls related to the study of polymers are addressed. Careful editing for consistency and cross-referencing among the chapters, high-quality graphics, worked-out examples, and numerous references to the specialist literature make "Applied Polymer Science" an essential reference for advanced students and practicing chemists, physicists, and engineers who want to solve problems with the use of polymeric materials.

Forensic DNA analysis plays a central role in the judicial system. A DNA sample can change the course of an investigation with immense consequences. Because DNA typing is recognized as the epitome of forensic science, increasing public awareness in this area is vital. Through several cases, examples and illustrations, this book explains the basic principles of forensic DNA typing, and how it integrates with law enforcement investigations and legal decisions. Written for a general readership, Understanding Forensic DNA explains both the power and the limitations of DNA analysis. This book dispels common misunderstandings regarding DNA analysis and shows how astounding match probabilities such as one-in-a-trillion are calculated, what they really mean, and why DNA alone never solves a case.

This book is focused on the current status of industrial pollution, its source, characteristics, and management through various advanced treatment technologies. The book covers the recycle, reuse and recovery of waste for the production of value-added products. The book explores industrial wastewater pollution and its treatment through various advanced technologies and also the source and characteristics of solid waste and its management for environmental safety. It discusses new methods and technologies to combat the waste-related pollution and focuses on the use of recycled products. This book is of value to upcoming students, researchers, scientists, industry persons and professionals in the field of environmental science and engineering, microbiology, biotechnology, toxicology, further it is useful for global and local authorities and policy makers responsible for the management of liquid and solid wastes.

Basic principles of applied life sciences such as recombinant DNA technology is used in most life sciences industries marketing bio-formulations for designing more effective protein-based drugs, such as erythropoietin and fast-acting insulin etc. In recent times genetically engineered host cells from mammal, animal and plants are also being used in life sciences industries to manufacture biologics. This book discusses the most basic as well advanced issues on biological products for successfully managing a life sciences industry. It elucidates the life cycle of biological molecules, right from the conceptual development of different types of biopolymers, and their subsequent transfer from the conical flasks in laboratory to life sciences industries for large scale production and marketing. It focuses on sustainable longevity in the life cycle of commercial biopolymers. Cumulative facts and figures in this volume would immensely help in inspiring life sciences industry promoters to monitor value chain transfer process of biologics for better profitability. Additionally, it would serve as a perusal document for the students and researchers interested in entrepreneurial ventures or having their own start-up projects for the commercialization of biologics.

Taking advantage of recent advances throughout the sciences, Matthew Hedman brings the distant past closer to us than it has ever been. Here, he shows how scientists have determined the age of everything from the colonization of the New World over 13,000 years ago to the origin of the universe nearly fourteen billion years ago.
Hedman details, for example, how interdisciplinary studies of the Great Pyramids of Egypt can determine exactly when and how these incredible structures were built. He shows how the remains of humble trees can illuminate how the surface of the sun has changed over the past ten millennia. And he also explores how the origins of the earth, solar system, and universe are being discerned with help from rocks that fall from the sky, the light from distant stars, and even the static seen on television sets.
Covering a wide range of time scales, from the Big Bang to human history, "The Age of Everything" is a provocative and far-ranging look at how science has determined the age of everything from modern mammals to the oldest stars, and will be indispensable for all armchair time travelers.
"We are used to being told confidently of an enormous, measurable past: that some collection of dusty bones is tens of thousands of years old, or that astronomical bodies have an age of some billions. But how exactly do scientists come to know these things? That is the subject of this quite fascinating book. . . . As told by Hedman, an astronomer, each story is a marvel of compressed exegesis that takes into account some of the most modern and intriguing hypotheses."--Steven Poole, "Guardian """ "Hedman is worth reading because he is careful to present both the power and peril of trying to extract precise chronological data. These are all very active areas of study, and as you read Hedman you begin to see how researchers have to be both very careful and incredibly audacious, and how much of our understanding of ourselves--through history, through paleontology, through astronomy--depends on determining the age of everything."--Anthony Doerr, "Boston"" Globe"

This volume provides a practical, intuitive approach to electroanalytical chemistry, presenting fundamental concepts and experimental techniques without the use of technical jargon or unnecessarily extensive mathematics. This edition offers new material on ways of preparing and using microelectrodes, the processes that govern the voltammetric behavior of microelectrodes, methods for characterizing chemically modified electrodes, electrochemical studies at reduced temperatures, and more. The authors cover such topics as analog instrumentation, overcoming solution resistance with stability and grace in potentiostatic circuits, conductivity and conductometry, electrochemical cells, carbon electrodes, film electrodes, microelectrodes, chemically modified electrodes, mercury electrodes, and solvents and supporting electrolytes.

The scientific method delivers prosperity, yet scientific practice has become subject to corrupting influences from within and without the scientific community. This essential reference is intended to help remedy those threats. The authors identify eight essential criteria for the practice of science and provide checklists to help avoid costly failures in scientific practice. Not only for scientists, this book is for all stakeholders of the broad enterprise of science. Science administrators, research funders, journal editors, and policymakers alike will find practical guidance on how they can encourage scientific research that produces useful discoveries. Journalists, commentators, and lawyers can turn to this text for help with assessing the validity and usefulness of scientific claims. The book provides practical guidance and makes important recommendations for reforms in science policy and science administration. The message of the book is complemented by Nobel Laureate Vernon L. Smith's foreword, and an afterword by Terence Kealey.

Molecular plant pathology has directly benefited from advances in modern molecular techniques. These techniques have been applied both to pathogen and plant, enhancing our understanding of the organisms themselves and of the complex interactions which determine compatibility between them and their host plants. This new book and its companion volume represent the first comprehensive guide to the latest molecular techniques as well as the established approaches to the subject. Detailed protocols are included which address a wide range of investigations from plant pathogen isolation and culture, through physiology and biochemistry, to techniques for localizing genes and their products within the cells of the infected plants. Topics in Volume I include pathogens and pathogen manipulation, nucleic acid isolation and hybridization techniques, analysis of gene expression, and in situ hybridization. Researchers in plant science, molecular biology, and related areas will find the two volumes of Molecular Plant Pathology to be an invaluable experimental resource for this exciting and fast moving field, providing a wealth of easy-to-follow protocols supported by expert advice and guidance.

This book is the final part of a trilogy of volumes covering the practical aspects of ligand interactions, biochemistry, and effector mechanisms of signal-transducing receptors. The book focuses on the measurement of receptor binding interactions both at equilibrium and from the kinetic point of view. In addition to descriptions of the most important techniques for the performance of ligand binding assays to both membrane-bound and solubilized receptors. A systematic effort has been made to provide insight into the strategy and tactics of receptor binding assays, and the conditions which must be satisfied if artifacts are to be avoided and interpretable data obtained. Since the behaviuor of even the simplest receptor systems can be surprisingly complex, an attempt has been made to provide some computational tools which allow complications to be predicted and handled or avoided. Ligand binding assays are very widely used, not least in drug screening and development. This volume will help the practitioner to choose an appropriate ligand, make a suitable receptor preparation, design and perform a well thought-out set of assays, and analyse the data obtained correctly.

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

The scientific method delivers prosperity, yet scientific practice has become subject to corrupting influences from within and without the scientific community. This essential reference is intended to help remedy those threats. The authors identify eight essential criteria for the practice of science and provide checklists to help avoid costly failures in scientific practice. Not only for scientists, this book is for all stakeholders of the broad enterprise of science. Science administrators, research funders, journal editors, and policymakers alike will find practical guidance on how they can encourage scientific research that produces useful discoveries. Journalists, commentators, and lawyers can turn to this text for help with assessing the validity and usefulness of scientific claims. The book provides practical guidance and makes important recommendations for reforms in science policy and science administration. The message of the book is complemented by Nobel Laureate Vernon L. Smith's foreword, and an afterword by Terence Kealey.

This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data. Beginning with up-to-date sequencing methods, the book continues with sections covering methods for deep phylogenetic analysis, the role of metagenomic data in understanding the genomics of the many yet uncultured bacteria, progress in genome-to-phenome inference, as well as computational genomic tools. Written for the highly successful Methods in Molecular Biology series, chapters include the type of practical detail necessary for reproducible results in the lab. Authoritative and up-to-date, Bacterial Pangenomics: Methods and Protocols, Second Edition serves as an ideal guide for both highly qualified investigators in bacterial genomics and for less experienced researchers, including students and teachers, who could use a reference for approaching genomic analysis and genome data.

A definitive work on ESR and polymer science by today's leading authorities

The past twenty years have seen extraordinary advances in electron spin resonance (ESR) techniques, particularly as they apply to polymeric materials. With contributions from over a dozen of the world's top polymer scientists, Advanced ESR Methods in Polymer Research is the first book to bring together all the current trends in this exciting field into one comprehensive reference.
Part I establishes the fundamentals of ESR, from experimental techniques to data analysis, and serves as a valuable overview for the beginning ESR student. Part II introduces the broad range of ESR applications to polymeric systems, including living radical polymerization, block copoly-mers, polymer solutions, ion-containing polymers, polymer lattices, membranes in fuel cells, degradation, polymer coatings, dendrimers, and conductive polymers. By exposing readers to the great potential of ESR, the authors hope to encourage more extensive application of these methods.

Fermentation: A Practical Approach is a collection of methods and techniques covering the setting up and use of fermentation units in academic research and industrial laboratories. The emphasis is on the breadth of usage of small-scale fermenters and the interdisciplinary nature of fermentation itself. The topics covered include fermentation modelling, sterilization, and instrumentation. This area of research has many important industrial applications, as evidenced by the affiliations of the authors (Hoechst, ICI, Beecham Pharmaceuticals).

FROM REVIEWS OF THE SERIES
"Reviews in Computational Chemistry remains the most valuable reference to methods and techniques in computational chemistry."
-JOURNAL OF MOLECULAR GRAPHICS AND MODELLING
"One cannot generally do better than to try to find an appropriate article in the highly successful Reviews in Computational Chemistry. The basic philosophy of the editors seems to be to help the authors produce chapters that are complete, accurate, clear, and accessible to experimentalists (in particular) and other nonspecialists (in general)."
-JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Combined fields of Microbiology and Nanotechnology have been most successful in providing novel solutions for protecting the health of humans and environment. This book covers the implications of nano-strategies to combat bacterial pathogens, applications of nanotechniques in microbiology, and innovative advances in the area of medical microbiology. Contents are divided into three sections -- Nanoscience in controlling bacterial pathogens, Nanoscience in Microbiology, Medical Microbiology. This volume is going to provide timely information about the technological advances of Nanoscience in the domain of Microbiology, with a special emphasis on Pathobiology. The book is a useful read for students and researchers in microbiology, nanotechnology and medical microbiology.

This book discusses fundamentally new biomedical imaging methods, such as holography, holographic and resonant interferometry, and speckle optics. It focuses on the development of holographic interference microscopy and its use in the study of phase objects such as nerve and muscle fibers subjected to the influence of laser radiation, magnetic fields, and hyperbaric conditions. The book shows how the myelin sheath and even the axon itself exhibit waveguide properties, enabling a fresh new look at the mechanisms of information transmission in the human body. The book presents theoretically and experimentally tested holographic and speckle-optical methods and devices used for investigating complex, diffusely scattering surfaces such as skin and muscle tissue. Additionally, it gives broad discussion of the authors' own original fundamental and applied research dedicated to helping physicians introduce new contact-less methods of diagnosis and treatment of diseases of the cardiovascular and neuromuscular systems into medical practice. The book is aimed at a broad spectrum of scientific specialists in the fields of speckle optics, holography, laser physics, morphology and cytochemistry, as well as medical professionals such as physiologists, neuropathologists, neurosurgeons, cardiologists and dentists.

This book presents a detailed look at experimental and computational techniques for accurate structure determination of free molecules. The most fundamental property of a molecule is its structure - it is a prerequisite for determining and understanding most other important properties of molecules. The determination of accurate structures is hampered by a myriad of factors, subjecting the collected data to non-negligible systematic errors. This book explains the origin of these errors and how to mitigate and even avoid them altogether. It features a detailed comparison of the different experimental and computation methods, explaining their interplay and the advantages of their combined use. Armed with this information, the reader will be able to choose the appropriate methods to determine - to a great degree of accuracy - the relevant molecular structure.

Many students find it daunting to move from studying environmental science, to designing and implementing their own research proposals. This book provides a practical introduction to help develop scientific thinking, aimed at undergraduate and new graduate students in the earth and environmental sciences. Students are guided through the steps of scientific thinking using published scientific literature and real environmental data. The book starts with advice on how to effectively read scientific papers, before outlining how to articulate testable questions and answer them using basic data analysis. The Mauna Loa CO2 dataset is used to demonstrate how to read metadata, prepare data, generate effective graphs and identify dominant cycles on various timescales. Practical, question-driven examples are explored to explain running averages, anomalies, correlations and simple linear models. The final chapter provides a framework for writing persuasive research proposals, making this an essential guide for students embarking on their first research project.

Lungenfunktionsuntersuchung, inklusive der arteriellen Blutgasanalyse, und Spiroergometrie zahlen zu den etablierten Diagnoseverfahren. Der Autor behandelt die physiologischen und physikalischen Grundlagen und vermittelt die Analyse und klinische Interpretation der Befunde: Messwerte, typische Befundmuster und ihre Deutung. Fur die 3.Auflage wurden Definitionen und Referenzwerte aktualisiert. Neu sind Themen wie die Anwendung der Wassermann schen Neunfeldertafel. Der Band enthalt die neueste Demonstrationsversion der Software Pulmopret light.

This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.