This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Circadian Regulation: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. Chapters 3, 4, 8, and 17 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

For students, DIY hobbyists, and science buffs, who can no longer get real chemistry sets, this one-of-a-kind guide explains how to set up and use a home chemistry lab, with step-by-step instructions for conducting experiments in basic chemistry - not just to make pretty colors and stinky smells, but to learn how to do real lab work: purify alcohol by distillation; produce hydrogen and oxygen gas by electrolysis; smelt metallic copper from copper ore you make yourself; analyze the makeup of seawater, bone, and other common substances; synthesize oil of wintergreen from aspirin and rayon fiber from paper; perform forensics tests for fingerprints, blood, drugs, and poisons; and much more.From the 1930s through the 1970s, chemistry sets were among the most popular Christmas gifts, selling in the millions. But two decades ago, real chemistry sets began to disappear as manufacturers and retailers became concerned about liability. "The Illustrated Guide to Home Chemistry Experiments" steps up to the plate with lessons on how to equip your home chemistry lab, master laboratory skills, and work safely in your lab.The bulk of this book consists of 17 hands-on chapters that include multiple laboratory sessions on the following topics: Separating Mixtures; Solubility and Solutions; Colligative Properties of Solutions; Introduction to Chemical Reactions and Stoichiometry; Reduction-Oxidation (Redox) Reactions; Acid-Base Chemistry; Chemical Kinetics; Chemical Equilibrium and Le Chatelier's Principle; Gas Chemistry; Thermochemistry and Calorimetry; Electrochemistry; Photochemistry; Colloids and Suspensions; Qualitative Analysis; Quantitative Analysis; Synthesis of Useful Compounds; and Forensic Chemistry. With plenty of full-color illustrations and photos, Illustrated Guide to Home Chemistry Experiments offers introductory level sessions suitable for a middle school or first-year high school chemistry laboratory course, and more advanced sessions suitable for students who intend to take the College Board Advanced Placement (AP) Chemistry exam. A student who completes all of the laboratories in this book will have done the equivalent of two full years of high school chemistry lab work or a first-year college general chemistry laboratory course. This hands-on introduction to real chemistry - using real equipment, real chemicals, and real quantitative experiments - is ideal for the many thousands of young people and adults who want to experience the magic of chemistry.

The careful design of experiments lies at the core of good research. Experimental Design for the Life Sciences equips you with the skills you need to effectively design experiments, making this essential aspect of the research process readily understandable. It demonstrates how good experimental design relies on clear thinking and biological understanding, not mathematical or statistical complexity. With a refreshingly approachable and articulate style, the book walks you through the considerations that go into designing an experiment in clear, practical terms. Using examples drawn from across the life sciences - from ecology, biochemistry, molecular biology, genetics, and health sciences - the authors illustrate how these concepts are applied within the broad context of real biological research. Online Resource Centre The Online Resource centre to accompany Experimental Design for the Life Sciences features: For students: * Self-test questions and answers * Additional examples * Supplementary sections discuss complex concepts and statistical issues in more depth * Links to useful websites and free software For lecturers: * Suggested course structures, complete with practical exercises * Figures from the book, available to download

The fundamental principles of the scientific method are essential for enhancing perspective, increasing productivity, and stimulating innovation. These principles include deductive and inductive logic, probability, parsimony and hypothesis testing, as well as science's presuppositions, limitations, ethics and bold claims of rationality and truth. The examples and case studies drawn upon in this book span the physical, biological and social sciences; include applications in agriculture, engineering and medicine; and also explore science's interrelationships with disciplines in the humanities such as philosophy and law. Informed by position papers on science from the American Association for the Advancement of Science, National Academy of Sciences and National Science Foundation, this book aligns with a distinctively mainstream vision of science. It is an ideal resource for anyone undertaking a systematic study of scientific method for the first time, from undergraduates to professionals in both the sciences and the humanities.

The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.

Volume 7 Proceedings of the Eindhoven Conference June 1718 1968.

This book was first published in 2006. The standard model brings together two theories of particle physics in order to describe the interactions of subatomic particles, except those due to gravity. This book uses the standard model as a vehicle for introducing quantum field theory. In doing this the book also introduces much of the phenomenology on which this model is based. The book uses a modern approach, emphasizing effective field theory techniques, and contains brief discussions of some of the main proposals for going beyond the standard model, such as seesaw neutrino masses, supersymmetry, and grand unification. Requiring only a minimum of background material, this book is ideal for graduate students in theoretical and experimental particle physics. It concentrates on getting students to the level of being able to use this theory by doing real calculations with the minimum of formal development, and contains several problems.

The work of accident prevention in the lab begins with foresight. Discerning "close calls"—near accidents—early enough prevents them from turning into full-fledged mishaps, mishaps that cost time and money, and which could result in injury. Improving Safety in the Chemical Laboratory is an accident prevention handbook for the professional in the lab that shows how to detect and eliminate the causes of dangerous mishaps—and virtually "hazard proof" any lab environment. In unequivocally clear and practical terms, Improving Safety in the Chemical Laboratory, Second Edition offers detailed procedures—from precautionary labeling to simulated drills, safety inspections,and the preparation of a chemical hygiene plan—for the development of a safety-enhanced workplace. Reflecting, in part, the upgraded procedures now mandated by the OSHA Laboratory Standard in the USA, as well as the WHMIS regulations in Canada and the COSHH regulations in the United Kingdom, this newest edition offers unparalleled and up-to-date guidance on the fine points of hazard control, with new added material on managing and handling especially hazardous substances and personal protective equipment:

• The 95 percent solution: the list of causes of laboratory accidents
• Hazard categories: unsafe acts; unsafe conditions
• Selecting and maintaining personal protective conditions
• Accident handling
• Classes of fuels and fires
• Preventing and extinguishing fires
• Toxic effects of chemicals
• Recognition of and treatment for exposure
• Chemical specific safety protocol
• Storage of lab chemicals
• Safe disposal of hazardous waste
• Personal protective equipment in the laboratory
• Improving hood performance
• Designing safety into new or renovated laboratories

Passive acoustic monitoring is increasingly used by the scientific community to study, survey and census marine mammals, especially cetaceans, many of which are easier to hear than to see. PAM is also used to support efforts to mitigate potential negative effects of human activities such as ship traffic, military and civilian sonar and offshore exploration. Walter Zimmer provides an integrated approach to PAM, combining physical principles, discussion of technical tools and application-oriented concepts of operations. Additionally, relevant information and tools necessary to assess existing and future PAM systems are presented, with Matlab code used to generate figures and results so readers can reproduce data and modify code to analyse the impact of changes. This allows the principles to be studied whilst discovering potential difficulties and side effects. Aimed at graduate students and researchers, the book provides all information and tools necessary to gain a comprehensive understanding of this interdisciplinary subject.

Science teachers come in many varieties, but they share a common goal: to nurture learners. Over the past decade, we have learned a great deal about how to do this effectively. Of all this new (and some not so new) knowledge, what strikes me as most important is that learning occurs best within a context. Still, as obvious as that may seem, it is relatively rare in our high school science classrooms. The problem, of course, is that it is not easy to create a learning experience with hands-on relevance to the science under discussion. Science teachers, in addition to not having the the time, for the most part do not have the expertise or readily available resources. The solution lies in finding ways to bring scientists into the teaching/learning equation. Scientists teamed with teachers and their students represent a very real and rich opportunity to involve students in real science as practiced. Imagine a research book that gives examples of honest, science-research experiences for science-oriented students. What's more, imagine a book that includes examples where students are collaborating with scientists from all over the world on research projects, in person or via the Internet. Internet Linksfor Science Education does just that. It explores the role of the Internet and technol ogy in working student-scientist partnerships.

In der Lebensmittelhygiene spielt bisher die EinschAtzung krankhafter VerAnderungen in der KArpermuskulatur von Fischen im Vergleich zum mikrobiellen Verderb und der Kontamination mit pathogenen Erregern und Umweltchemikalien eine relativ untergeordnete Rolle. In diesem Buch werden die Fischarten der Welt aufgelistet, deren KArpermuskulatur pathologisch verAndert sein kann. Ganz A1/4berwiegend sind dafA1/4r Parasiten die Ursache. Eine Reihe dieser Parasiten kann beim Menschen nach dem Verzehr zu Erkrankungen fA1/4hren. Die Lebensmittelunternehmer und die Mitarbeiter der Kontroll- und HygienebehArden mA1/4ssen das Erscheinungsbild im Filet kennen, um es von Ahnlichen Abweichungen differenzialdiagnostisch unterscheiden zu kAnnen. Nach den hierbei relevanten Fragen des Lebensmittelrechts, der Verkehrsauffassung, der Anatomie und Physiologie und der allgemeinen Pathologie der Fischmuskulatur widmet sich das Buch schwerpunktmAAig den parasitAren Ursachen der MuskelverAnderungen. Diese sind A1/4berwiegend nach der Systematik der beteiligten Parasitenkategorien geordnet. AusfA1/4hrungen zu den Untersuchungsmethoden, deren Effizienz sowie zu MaAnahmen der Vermeidung eines Gesundheitsrisikos schlieAen das Buch ab. Ein zweisprachiges Glossar, sowie zusAtzliche Register A1/4ber Fischarten, Parasiten und deren wirbellose Zwischenwirte erleichtern die Handhabung.

Praise for the "First Edition"

"If you . . . want an up-to-date, definitive reference written by authors who have contributed much to this field, then this book is an essential addition to your library."
--"Journal of the American Statistical Association"

Fully updated to reflect the major progress in the use of statistically designed experiments for product and process improvement, "Experiments," Second Edition introduces some of the newest discoveries--and sheds further light on existing ones--on the design and analysis of experiments and their applications in system optimization, robustness, and treatment comparison. Maintaining the same easy-to-follow style as the previous edition while also including modern updates, this book continues to present a new and integrated system of experimental design and analysis that can be applied across various fields of research including engineering, medicine, and the physical sciences.

The authors modernize accepted methodologies while refining many cutting-edge topics including robust parameter design, reliability improvement, analysis of non-normal data, analysis of experiments with complex aliasing, multilevel designs, minimum aberration designs, and orthogonal arrays. Along with a new chapter that focuses on regression analysis, the Second Edition features expanded and new coverage of additional topics, including:

Expected mean squares and sample size determination

One-way and two-way ANOVA with random effects

Split-plot designs

ANOVA treatment of factorial effects

Response surface modeling for related factors

Drawing on examples from their combined years of working with industrial clients, the authors present many cutting-edge topics in a single, easily accessible source. Extensive case studies, including goals, data, and experimental designs, are also included, and the book's data sets can be found on a related FTP site, along with additional supplemental material. Chapter summaries provide a succinct outline of discussed methods, and extensive appendices direct readers to resources for further study.

"Experiments," Second Edition is an excellent book for design of experiments courses at the upper-undergraduate and graduate levels. It is also a valuable resource for practicing engineers and statisticians.

The functional anatomy of human somatosensory cortex is of both scientific and clinical interest. Scientifically, it provides insights in information processing in the human brain. Clinically, it helps to avoid neurological deficits by sparing essential brain regions during neurosurgical procedures adjacent to central fissure. In the present study the functional organization of the human somatosensory cortex was investigated with electrophysiological techniques using a combined approach of cortical stimulations and somatosensory evoked responses on electrocorticography, scalp-EEG, and magnetoencephalography. The spatiotemporal structure of the evoked response was studiedwith biophysical modeling techniqueswhich allowed identification of the three-dimensional intracerebral location, time activity, and interaction of the neuronal sources activated following peripheral somatosensory stimulation. Furthermore, the somatotopic organization ofhand and lip somatosensory cortex was investigated. The relative value of invasive (cortical stimulations and electrocorticography) and non-invasive (scalp-EEGand magnetoencephalography) was assessed. The combined use ofscalp-EEG and magnetoencephalography was useful to increase non-invasive localization accuracy. I want to thank several people who significantly contributed in completion of the present work. Univ. -Prof Dr. Luder Deecke, Chairman of the Neurological University Clinic, Vienna, Austria, supported me throughout my career at the Neurological University Clinic in Vienna since 1985. Dr. William W Sutherling, Associate Professor at the University of California, Los Angeles, who was my advisor during my stay from 1987-1989 at the Department ofNeurology, University of California, Los Angeles where most of the present work was done.

Maria Rentetzi surveys the experimental practices of radioactivity research in early-twentieth-century Vienna, focusing on radioactive materials, instruments, women's work in physics, and gendered skills. She shows how experimental cultures in radioactivity-scientific practices employed by gendered subjects who shared a certain material and epistemic style of research--were constructed and reshaped by socialist politics in Vienna at that time. She also explores the different ways experimental practices affected men and women in laboratory sciences. Rentetzi expands the notion of material culture to include not only instruments and objects but also materials that operated as both commodities and objects of scientific inquiry. She tells a multifaceted story of how purified radium ended up on laboratory benches and who extracted and isolated it from tons of residues; the individuals who designed experiments and instruments for probing radium's properties; and those who carried radium outside of the physics laboratory and into the clinic and medical amphitheatres. Rentetzi examines how the architecture of the laboratory affected men's and women's scientific work and the way in which its urban setting reflected assumptions about scientific cross-disciplinary collaborations. Following the circulation of radium and the pursuit of power through strategies of partnership and collaboration, Rentetzi redraws paths of scientific exchange and transfers the reader from scientific laboratories to hospitals and from academic to industrial sites.

The careful explanation of each step of the dissection, helpful diagrams and illustrations, and detailed discussion of the structure and function of each system in "Anatomy and Dissection of the Fetal Pig, Fifth Edition, " optimize the educational value of the dissection process. These laboratory exercises are sold separately or as a bound set. This popular series, which includes "Anatomy and Dissection of the Rat" and "Anatomy and Dissection of the Frog" is geared toward introductory courses in biology and comparative anatomy.
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This book should be on the shelf of every practising statistician who designs experiments. Good design considers units and treatments first, and then allocates treatments to units. It does not choose from a menu of named designs. This approach requires a notation for units that does not depend on the treatments applied. Most structure on the set of observational units, or on the set of treatments, can be defined by factors. This book develops a coherent framework for thinking about factors and their relationships, including the use of Hasse diagrams. These are used to elucidate structure, calculate degrees of freedom and allocate treatment subspaces to appropriate strata. Based on a one-term course the author has taught since 1989, the book is ideal for advanced undergraduate and beginning graduate courses. Examples, exercises and discussion questions are drawn from a wide range of real applications: from drug development, to agriculture, to manufacturing.

This book introduces the reader to the power of observation before, and sometimes instead of, experimental manipulation in the study of animal behavior. It starts with simple and easily accessible methods suitable for student projects, before going on to demonstrate the possibilities that now exist for far more sophisticated analyses of observational data. At a time when animal welfare considerations are attracting political as well as scientific debate, the potential for non-intrusive studies on animals is being increasingly recognized. Observation emerges as a valuable alternative approach, often yielding highly informative results in situations (such as on zoos, farms or for wild animals) where more invasive experimental techniques would be undesirable, unethical or just plain impossible. However, to justify its place alongside experimentation as a rigorous scientific method, observation needs to be just as disciplined and systematic and have just as much attention paid to project design in the way that observations are made and recorded.
Observing Animal Behaviour takes the reader through all these stages: from the initial observations, to the formulation of hypotheses, and their subsequent testing with further systematic observations. Although designed principally as a companion text for advanced undergraduate and students taking courses in animal behavior, this accessible text will be essential reading for anyone wanting to study animal behavior using observational methods rather than experimentation, and assumes no previous knowledge of animals, statistics or scientific method. It will be of particular relevance and use to those professional researchers and consultants inthe behavioral sciences who seek a compact but comprehensive introduction to the quantitative observation of animal behavior.

Medical researchers including doctors, nurses, medical students and allied health professionals are required to undertake a period of research as part of their career pathway. This book covers the main areas, allowing anyone to set up and complete research projects and this information can easily be translated to clinical projects. Written by international authors to provide a flavor from many institutions, the booka (TM)s appeal is cross-sectional, both at hospital and primary care levels worldwide.

Section A, the most important area in the book, covers the hardest step - setting up research (i.e. initial paperwork and funding projects). Section B is technical, covering modern laboratory techniques for laboratory-based research. Section C tackles data collation and analysis and the presentation of the results (oral and written).

The book is directed at all doctors, medical students, and individuals in related specialities, either in research or considering research (e.g. MD, PhD, MSc and Intercalated BSc).

This book aims to provide an introduction to the major techniques of chemoinformatics. It is the first text written specifically for this field. The first part of the book deals with the representation of 2D and 3D molecular structures, the calculation of molecular descriptors and the construction of mathematical models. The second part describes other important topics including molecular similarity and diversity, the analysis of large data sets, virtual screening, and library design. Simple illustrative examples are used throughout to illustrate key concepts, supplemented with case studies from the literature.

For most of the history of scientific endeavour, science has been recorded on paper. In this digital era, however, there is increasing pressure to abandon paper in favour of digital tools. Despite the benefits, there are barriers to the adoption of such tools, not least their usability. As the relentless development of technology changes the way we work, we need to ensure that the design of technology not only overcomes these barriers, but facilitates us as scientists and supports better practice within science. This book examines the importance of record-keeping in science, current record-keeping practices, and the role of technology for enabling the effective capture, reuse, sharing, and preservation of scientific data. Covering the essential areas of electronic laboratory notebooks (ELNs) and digital tools for recording scientific data, including an overview of the current data management technology available and the benefits and pitfalls of using these technologies, this book is a useful tool for those interested in implementing digital data solutions within their research groups or departments. This book also provides insight into important factors to consider in the design of digital tools such as ELNs for those interested in producing their own tools. Finally, it looks at the role of current technology and then considers how that technology might develop in the future to better support scientists in their work, and in capturing and sharing the scientific record.

This book was first published in 2006. The standard model brings together two theories of particle physics in order to describe the interactions of subatomic particles, except those due to gravity. This book uses the standard model as a vehicle for introducing quantum field theory. In doing this the book also introduces much of the phenomenology on which this model is based. The book uses a modern approach, emphasizing effective field theory techniques, and contains brief discussions of some of the main proposals for going beyond the standard model, such as seesaw neutrino masses, supersymmetry, and grand unification. Requiring only a minimum of background material, this book is ideal for graduate students in theoretical and experimental particle physics. It concentrates on getting students to the level of being able to use this theory by doing real calculations with the minimum of formal development, and contains several problems.

Prudent Practices in the Laboratory-the book that has served for decades as the standard for chemical laboratory safety practice-now features updates and new topics. This revised edition has an expanded chapter on chemical management and delves into new areas, such as nanotechnology, laboratory security, and emergency planning. Developed by experts from academia and industry, with specialties in such areas as chemical sciences, pollution prevention, and laboratory safety, Prudent Practices in the Laboratory provides guidance on planning procedures for the handling, storage, and disposal of chemicals. The book offers prudent practices designed to promote safety and includes practical information on assessing hazards, managing chemicals, disposing of wastes, and more. Prudent Practices in the Laboratory will continue to serve as the leading source of chemical safety guidelines for people working with laboratory chemicals: research chemists, technicians, safety officers, educators, and students. Table of Contents Front Matter 1 The Culture of Laboratory Safety 2 Environmental Health and Safety Management System 3 Emergency Planning 4 Evaluating Hazards and Assessing Risks in the Laboratory 5 Management of Chemicals 6 Working with Chemicals 7 Working with Laboratory Equipment 8 Management of Waste 9 Laboratory Facilities 10 Laboratory Security 11 Safety Laws and Standards Pertinent to Laboratories Bibliography APPENDIXES Appendix A: OSHA Laboratory Standard Appendix B: Statement of Task Appendix C: Committee Member Biographies Index

Flow cytometry is now well established in research laboratories and is gaining increasing use in clinical medicine and pathology. The technique enables multiple simultaneous light scatter and fluorescence measurements to be made at the individual cell level at very rapid rates and results in very large quantities of data being collected. Data, however, is just a series of numbers which have to be converted to information which, in turn, must be shown to have meaning. This is the most important single aspect of flow cytometry but it has received relatively little attention. One of the frequently voiced advantages of the technology is that it produces 'good statistics' because large numbers of cells have been analysed. However, it is not very often that confidence limits are placed on results, hence the reader has little or no feel for the inherent variability in the information produced. This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analysing dynamic processes. All those who use flow cytometry in their research will find this book an invaluable guide to interpreting the data produced by flow cytometers.

Maximize your skills and understanding with EXPERIMENTS IN GENERAL CHEMISTRY: INQUIRY AND SKILL BUILDING, Third Edition. The manual's 31 experiments include Skill Building, Guided Inquiry, and Open Inquiry experiments to provide maximum lab experience in the minimum amount of lab time. Each experiment includes prelab questions to help you prepare for the lab ahead of time and post-lab questions that lead you from data analysis to concept development to reinforce the core concepts of the lab.