The scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzymologists and may be unaware of some of the parameters that are important in a specific enzyme reaction.

The scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzymologists and may be unaware of some of the parameters that are important in a specific enzyme reaction.

A treatment of the experimental techniques and instrumentation most often used in nuclear and particle physics experiments as well as in various other experiments, providing useful results and formulae, technical know-how and informative details. This second edition has been revised, while sections on Cherenkov radiation and radiation protection have been updated and extended.

Professor John D. Roberts published a highly readable book on Molecular Orbital Calculations directed toward chemists in 1962. That timely book is the model for this book. The audience this book is directed toward are senior undergraduate and beginning graduate students as well as practicing bench chemists who have a desire to develop conceptual tools for understanding chemical phenomena. Although, ab initio and more advanced semi-empirical MO methods are regarded as being more reliable than HMO in an absolute sense, there is good evidence that HMO provides reliable relative answers particularly when comparing related molecular species. Thus, HMO can be used to rationalize electronic structure in 1t-systems, aromaticity, and the shape use HMO to gain insight of simple molecular orbitals. Experimentalists still into subtle electronic interactions for interpretation of UV and photoelectron spectra. Herein, it will be shown that one can use graph theory to streamline their HMO computational efforts and to arrive at answers quickly without the aid of a group theory or a computer program of which the experimentalist has no understanding. The merging of mathematical graph theory with chemical theory is the formalization of what most chemists do in a more or less intuitive mode. Chemists currently use graphical images to embody chemical information in compact form which can be transformed into algebraical sets. Chemical graph theory provides simple descriptive interpretations of complicated quantum mechanical calculations and is, thereby, in-itself-by-itself an important discipline of study.

Responding to the developments of the past twenty years, Les Kirkup has thoroughly updated his popular book on experimental methods, while retaining the extensive coverage and practical advice from the first edition. Many topics from that edition remain, including keeping a record of work, how to deal with measurement uncertainties, understanding the statistical basis of data analysis and reporting the results of experiments. However, with new technologies influencing how experiments are devised, carried out, analyzed, presented and reported, this new edition reflects the digital changes which have taken place and the increased emphasis on the importance of communication skills in reporting results. Bringing together key elements of experimental methods into one coherent book, it is perfect for students seeking guidance with their experimental work, including how to acquire, analyse and present data. Exercises, worked examples and end-of-chapter problems are provided throughout the book to reinforce fundamental principles.

Geophysical measurements are not done for the sake of art only. The ultimategoal is to solve some well-defined geological, tectonical or structural problems. For this purpose, the data have to be interpreted, translated, into a physical model of the subsurface. ... This book describes some ofthe most important common features of different geophysical data sets. (fromthe Introduction) Users at universities but also practitioners in exploration, physics or environmental sciences, wherever signal processing is necessary, will benefit from this textbook.

Nucleic acid hybridization techniques allow the detection of specific DNA or RNA sequences. This book is a clear and concise guide to the techniques used for preparing DNA and RNA for membrane hybridization. These include Southern blotting of DNA, northern blotting of RNA, dot/slot blotting, Benton-and-Davis screening of recombinant bacteriophage and Grunstein-Hogness screening of recombinant plasmids. It also discusses the pros and cons of using nitrocellulose filters and nylon membranes in these procedures. The book demystifies the laboratory manuals by explaining the rationale for each step in the published protocols and points out potential pitfalls with tips on how to avoid them.
The ideal support for newcomers - provides the answers to all the questions you want to ask.

Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

This history of the thermometer includes controversy about its invention, the story of different scales, Fahrenheit and centigrade, and the history of the gradual scientific then popular understanding of the concept of temperature. Not until 1800 did people interested in thermometers begin to see clearly what they were measuring, and the impetus for improving thermometry came largely from study of the weather--the liquid-in-glass thermometer became the meteorologist's instrument before that of the chemist or physicist. This excellent introductory study follows the development of indicating and recording thermometers until recent times, emphasizing meteorological applications.

Purification of Laboratory Chemicals, Eighth Edition, tabulates methods taken from literature for purifying thousands of individual commercially available chemicals. To help in applying this information, the more common processes currently used for purification in chemical laboratories and new methods are discussed. For dealing with substances not separately listed, a chapter is included setting out the usual methods for purifying specific classes of compounds.

The Fundamentals of Scientific Research: An Introductory Laboratory Manual is a laboratory manual geared towards first semester undergraduates enrolled in general biology courses focusing on cell biology. This laboratory curriculum centers on studying a single organism throughout the entire semester Serratia marcescens, or S. marcescens, a bacterium unique in its production of the red pigment prodigiosin. The manual separates the laboratory course into two separate modules. The first module familiarizes students with the organism and lab equipment by performing growth curves, Lowry protein assays, quantifying prodigiosin and ATP production, and by performing complementation studies to understand the biochemical pathway responsible for prodigiosin production. Students learn to use Microsoft Excel to prepare and present data in graphical format, and how to calculate their data into meaningful numbers that can be compared across experiments. The second module requires that the students employ UV mutagenesis to generate hyper-pigmented mutants of S. marcescens for further characterization. Students use experimental data and protocols learned in the first module to help them develop their own hypotheses, experimental protocols, and to analyze their own data. Before each lab, students are required to answer questions designed to probe their understanding of required pre-laboratory reading materials. Questions also guide the students through the development of hypotheses and predictions. Following each laboratory, students then answer a series of post-laboratory questions to guide them through the presentation and analysis of their data, and how to place their data into the context of primary literature. Students are also asked to review their initial hypotheses and predictions to determine if their conclusions are supportive. A formal laboratory report is also to be completed after each module, in a format similar to that of primary scientific literature. The Fundamentals of Scientific Research: An Introductory Laboratory Manual is an invaluable resource to undergraduates majoring in the life sciences.

TO VEGETATION ANALYSIS Principles, practice and interpretation D.R.CAUSTON Department of Botany and Microbiology, University College of Wales, Aberystwyth London UNWIN HYMAN Boston Sydney Wellington (c) D. R. Causton, 1988 This book is copyright under the Berne Convention. No reproduction without permission. All rights reserved. Published by the Academic Division of Unwin Hyman Ltd 15/17 Broadwick Street, London W1V 1FP, UK Allen & Unwin Inc., 8 Winchester Place, Winchester, Mass. 01890, USA Allen & Unwin (Australia) Ltd, 8 Napier Street, North Sydney, NSW 2060, Australia Allen & Unwin (New Zealand) Ltd in association with the Port Nicholson Press Ltd, 60 Cambridge Terrace, Wellington, New Zealand First published in 1988 British Library Cataloguing in Publication Data Causton, David R. An introduction to vegetation analysis: principles, practice and intepretation. 1. Botany-Ecology-Mathematics I. Title 581.5'247 QK901 ISBN-13: 978-0-04-581025-3 e-ISBN-13: 978-94-011-7981-2 DOl: 10.1007/978-94-011-7981-2 Library of Congress Cataloging-in-Publication Data Causton, David R. An introduction to vegetation analysis. Bibliography: p. Includes index. 1. Botany-Ecology-Methodology. 2. Plant communities-Research-Methodology. 3. Vegetation surveys. 4. Vegetation classification. I. Title. QK90I.C33 1987 581.5 87-19327 ISBN-13: 978-0-04-581025-3 Typeset in 10 on 12 point Times by Mathematical Composition Setters Ltd, Salisbury and Biddies of Guildford Preface This book has been written to help students and their teachers, at various levels, to understand the principles, some of the methods, and ways of interpreting vegetational and environmental data acquired in the field.

This definitive new book should appeal to everyone who produces, uses, or evaluates scientific data. Ensures accuracy and reliability. Dr. Taylor's book provides guidance for the development and implementation of a credible quality assurance program, plus it also provides chemists and clinical chemists, medical and chemical researchers, and all scientists and managers the ideal means to ensure accurate and reliable work. Chapters are presented in a logical progression, starting with the concept of quality assurance, principles of good measurement, principles of quality assurance, and evaluation of measurement quality. Each chapter has a degree of independence so that it may be consulted separately from the others.

Many chemists - especially those most brilliant in their field - fail to appreciate the power of planned experimentation. They dislike the mathematical aspects of statistical analysis. In addition, these otherwise very capable chemists also dismissed predictive models based only on empirical data. Ironically, in the hands of subject matter experts like these elite chemists, the statistical methods of mixture design and analysis provide the means for rapidly converging on optimal compositions. What differentiates Formulation Simplified from the standard statistical texts on mixture design is that the authors make the topic relatively easy and fun to read. They provide a whole new collection of insighful original studies that illustrate the essentials of mixture design and analysis. Solid industrial examples are offered as problems at the end of many chapters for those who are serious about trying new tools on their own. Statistical software to do the computations can be freely accessed via a web site developed in support of this book.

This is the first book devoted to the use of X-ray beam techniques to study magnetic properties of materials. It covers both experimental and theoretical issues. The three main topics are dichroism, elastic scattering (both non-resonant and resonant diffraction) and spectroscopy. In the past decade there has been an expansion of activity in the field, driven by the availability of intense, tuneable and highly polarized X-ray beams from synchrtron facilities. The pace of events is likely to continue with the start of new (3rd generation) facilities, including the European Synchrotron Radiation Facility, Grenoble, and the Advanced Light Source, Argonne National Laboratory. USA.

This book focuses on the use of novel electron microscopy techniques to further our understanding of the physics behind electron-light interactions. It introduces and discusses the methodologies for advancing the field of electron microscopy towards a better control of electron dynamics with significantly improved temporal resolutions, and explores the burgeoning field of nanooptics - the physics of light-matter interaction at the nanoscale - whose practical applications transcend numerous fields such as energy conversion, control of chemical reactions, optically induced phase transitions, quantum cryptography, and data processing. In addition to describing analytical and numerical techniques for exploring the theoretical basis of electron-light interactions, the book showcases a number of relevant case studies, such as optical modes in gold tapers probed by electron beams and investigations of optical excitations in the topological insulator Bi2Se3. The experiments featured provide an impetus to develop more relevant theoretical models, benchmark current approximations, and even more characterization tools based on coherent electron-light interactions.

TO THE THEORY OF THE RAMAN EFFECT by J. A. KONINGSTEIN Carleton University, Ottawa, Canada D. REIDEL PUBLISHING COMPANY DORDRECHT-HOLLAND Library of Congress Catalog Card Number 72-77876 ISBN-13: 978-90-277-0276-0 e-ISBN-13: 978-94-010-2901-8 001: 10. 1007/978-94-010-2901-8 All Rights Reserved Copyright (c) 1972 by D. Reidel Publishing Company, Dordrecht, Holland Softcover reprint of the hardcover I st edition 1972 No part of this book may be reproduced in any form, by print, photoprint, microfilm, or any other means, without written permission from the publisher to M. G. INTRODUCTION This book is written particularly for chemists. Being one myself, I have on several occasions tried to find a book where the theory of molecular Raman spectroscopy is treated, and not being able to find one which satisfactorily answered the questions I wanted to see answered, I decided to try to write a book on it myself. Back in the middle fifties I was shown a Raman spectrum for the first time: some faint lines on a photographic plate. In the fall of 1971, during a visit to Moscow, I vividly remembered that spectrum when the son of Mandel'shtam showed me the first spectrum taken in Russia by his father and Landsberg in 1928. The spectrum of quartz photographed during January and February of that year showed the presence of some faint new lines and in later exposures these lines became stronger and stronger.

TO THE THEORY OF THE RAMAN EFFECT by J. A. KONINGSTEIN Carleton University, Ottawa, Canada D. REIDEL PUBLISHING COMPANY DORDRECHT-HOLLAND Library of Congress Catalog Card Number 72-77876 ISBN-13: 978-90-277-0276-0 e-ISBN-13: 978-94-010-2901-8 001: 10. 1007/978-94-010-2901-8 All Rights Reserved Copyright (c) 1972 by D. Reidel Publishing Company, Dordrecht, Holland Softcover reprint of the hardcover I st edition 1972 No part of this book may be reproduced in any form, by print, photoprint, microfilm, or any other means, without written permission from the publisher to M. G. INTRODUCTION This book is written particularly for chemists. Being one myself, I have on several occasions tried to find a book where the theory of molecular Raman spectroscopy is treated, and not being able to find one which satisfactorily answered the questions I wanted to see answered, I decided to try to write a book on it myself. Back in the middle fifties I was shown a Raman spectrum for the first time: some faint lines on a photographic plate. In the fall of 1971, during a visit to Moscow, I vividly remembered that spectrum when the son of Mandel'shtam showed me the first spectrum taken in Russia by his father and Landsberg in 1928. The spectrum of quartz photographed during January and February of that year showed the presence of some faint new lines and in later exposures these lines became stronger and stronger.

Spark scientific curiosity from a young age with this six-level course through an enquiry-based approach and active learning. Collins International Primary Science fully meets the requirements of the Cambridge Primary Science Curriculum Framework from 2020 and has been carefully developed for a range of international contexts. The course is organised into four main strands: Biology, Chemistry, Physics and Earth and Space and the skills detailed under the ‘Thinking and Working Scientifically’ strand are introduced and taught in the context of those areas. For each Teacher’s Guide at Stages 1 to 6, we offer: A comprehensive Teacher’s Guide is easy to follow with a clear and consistent lesson plan layout, including built in continuous assessment The Teacher’s Guide Plus ebook includes components such as slideshows, video clips, additional photographs and interactive activities Earth and Space content covers the new curriculum framework Thinking and Working Scientifically deepens and enhances the delivery of Science skills Includes practical activities that don’t require specialist equipment or labs Scaffolding allows students of varying abilities to work with common content and meet learning objectives Supports Cambridge Global Perspectives™ with activities that develop and practise key skills Provides teacher support as part of a set of resources for the Cambridge Primary Science curriculum framework (0097) from 2020 This series is endorsed by Cambridge Assessment International Education to support the new curriculum framework 0097 from 2020.

In addition to the issues constituting the basis of microbial biotechnology, such as microorganisms culturing or their use in industry and environmental protection, the book includes modern analytical techniques known as "omics", as well as digital techniques used to record adverse changes in the environment those resulting from the harmful activity of bacteria and fungi. A color atlas (Chapter 8) with photos of the fungi discussed in each chapter was also included. The book was prepared with the use of many years of scientific and didactic experience of the authors who conduct classes in various fields and specializations of natural sciences. This resulted in the interdisciplinary nature of the publication. It will be useful not only for PhD students and students of biotechnology and microbiology, but also environmental protection, ecological biotechnology, urban revitalization, as well as all those interested in applying the latest achievements of these areas of science in practice.

Data Fitting in the Chemical Sciences Peter Gans, School of Chemistry, The University of Leeds, Leeds, UK Data fitting is a technique of central importance in modern experimental science. It is the means by which data is tested against a model of the experimental system, be it a theoretical or empirical model. In this book an all-round approach is adopted in which the first stage of data-fitting is seen as data collection, the second is numerical processing and the third a critical evaluation of the 'goodness' of fit in both statistical and common sense terms. Each stage is considered in detail: the sources and nature of experimental errors; the theory of least-squares fitting; probability theory; hypothesis testing, and the application of scientific criteria. The theory is complemented by three chapters on a wide range of applications. The emphasis of this book is on methodology: why certain procedures are preferred rather than how any one procedure is implemented. The author aims to assist people in extracting from their data its full information content, i.e. to use their data, not abuse it.

Innovations in crystallographic instrumentation and the rapid development of methods of diffraction measurement have led to a vast improvement in our ability to determine crystal and molecular structure. This up-to-date resource will allow the reader to harness the potential of X-ray diffraction instruments. Different sources of X-radiation used in crystallography are introduced, including synchrotron radiation, as well as a systematic review of detectors for X-rays and basic instruments for single crystal and powder diffractometry. The principles of the diffraction experiment are discussed and related to their practical application with a comparative description of different scan procedures. Diffraction data collection and processing are also reviewed and methods for error correction are described. This book will provide a useful guide for researchers and students starting in this area of science, as well as skilled crystallographers.