This issue of Immunology and Allergy Clinics of North America, Guest Edited by Amy D. Klion, MD and Princess U. Ogbogu, MD, is devoted to Eosinophil-Associated Disorders. Drs. Klion and Ogbogu have assembled a group of expert authors to review the following topics: Evaluation and Differential Diagnosis of Persistent Marked Eosinophilia; Spectrum of Eosinophilic End Organ Manifestations; Eosinophilia in GI disorders; Eosinophilia in Hematologic Disorders; Eosinophilia in Rheumatologic/Vascular Disorders; Eosinophilia in Pulmonary Disorders; Eosinophilia in Infectious Diseases; Eosinophilia associated with Immune Deficiency and Immune Dysregulation; Eosinophilia in Dermatologic disorders; Management of Hypereosinophilic Syndromes; and Novel Therapies for Eosinophilic Disorders.

Granulomatous disorders represent a unique group of diseases both non-infectious and infectious that require the utmost clinical pathologic correlation combined with a keen sense of inquiry for underlying systemic disease. Some of these diseases are common and some are rare; however all dermatologists need to diagnose and manage these diseases whether common or rare. This issue of Dermatology Clinics "Granulomatous Disorders of the Adult Skin" supplies the readers with a comprehensive, up to date, and evidence based review of multiple granulomatous disorders (i.e. palisdaing, epitheliod, xanthomatous, casseating, supprative, vasculitic, lymphoproliferative, foreign body). Dermatologists need to be able to differentiate the entities, evaluate patients for specific underlying systemic diseases (diabetes to cancer) and treat with a wide range of immunosuppressant to anti-infectious agents. This issue will help the dermatologist learn the most current etiopathogenesis, clinical/systemic manifestations and therapeutic modalities for these diseases. Other specialists who would find the information useful: Rheumatologists, Infectious Disease specialists, Hematologists/Oncologists, Endocrinologists, Pulmonologists, Oral-maxillary surgeons, and Pharmacists.

This volume provides an overview of the field and practical hints for vaccinologists in academia and industry. Chapters provide protocols on self-replicating RNA vectors, non-replication mRNA vectors, adjuvantation and delivery, and preclinical and clinical development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Vaccines: Methods and Protocols aims to increased collaboration on RNA vaccines between basic and applied scientists in academia, government, and industry to develop future solutions for today's challenges.

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot's sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users."

In the current edition, Selenium: Its Molecular Biology and Role in Human Health expands extensively on the previous editions providing readers with the most significant advances in the rapidly developing selenium field. Evidence from epidemiology and veterinary science supports the essential role of selenium in (human) health, but its split personality in both preventing and supporting cancer and also in promoting insulin resistance has become more clearly defined. The pivotal role of glutathione peroxidase 4 in a new process of programmed cell death, ferroptosis, brings new impetus to the field. Recently defined mutations in selenoprotein and biosynthesis factor genes have been identified in patients, and the resulting disorders further emphasize the significance of selenoproteins in human health. The mechanism of selenoprotein biosynthesis, the functions of selenoproteins, and the roles of dietary selenium have been further elucidated, and new regulatory mechanisms involving selenoproteins discovered. The book, therefore, covers the breadth of current selenium research. With up-to-date chapters written by leaders in their fields, it serves as an invaluable resource for novices as well as specialists.

This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 1: Vaccines for Human Diseases has an introductory section on how vaccines impacted diseases, the immunological mechanism of vaccines, future challenges for vaccinologists, and current trends in vaccinology. The design of human vaccines for viral, bacterial, fungal, parasitic and prion diseases as well as vaccines for drug abuse, allergy, and tumor vaccines are also described in this volume. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Vaccine Design: Methods and Protocols, Volume 1: Vaccines for Human Diseases aims to ensure successful results in the further study of this vital field.

The type I interferon (IFN) signaling pathway is well recognized as a pathway activated by viral infections. It is activated by a variety of microbial pattern recognition receptors including the Toll-like receptors, NOD-like receptors and several cytosolic receptors. Activation of the type I IFN pathway leads to the production of both antiviral factors and products that influence immune cell function. More recently it has been shown that bacteria are also capable of activating this pathway. Bacterial Activation of Type I Interferons reviews both the current understanding of how different bacterial species are able to activate this pathway as well as the influence type I IFNs have on the outcome to infection. Several different bacterial species are covered, spanning Gram positive and Gram negative, intracellular, extracellular, and different host infection sites. An introduction to the pathogenesis of each organism is provided, and the signaling molecules involved in the activation of the type I IFN pathway and the role it plays in animal infection models are also covered.

Developing Costimulatory Molecules for Immunotherapy of Diseases highlights the novel concept of reverse costimulation and how it can be effectively exploited to develop immunotherapy using either humanized antibodies against CD80, CD86, and other costimulatory molecules or CD28 fusinogenic proteins in the treatment of diseases, including allergies, asthma, rheumatoid arthritis, multiple sclerosis, lupus nephritis, severe psoriasis, vulgaris tuberculosis, thopoid, transplantation therapeutic, cancer, and inflammation. The text aims to provide the latest information on the complex roles and interactions within the CD28 and B7 costimulatory families, with the hope that targeting these families will yield new therapies for the treatment of inflammation, autoimmunity, transplantation, cancer, and other infectious diseases.

This book sheds new light on "inducible" lymphoid organs (ILOs): antigen presentation sites that are generated de novo in peripheral tissues under various pathogenic conditions. Accomplished immunologists demonstrate that the physiological role of these ILOs is completely different from that of central lymphoid organs, i.e., the lymph nodes or spleen. In addition to the central organs, the ILOs are considered essential structures for the efficient elicitation of adaptive immune responses in lesions. The respective chapters highlight examples from multiple sites, e.g. the skin, lung, intestinal tract, genital tract, the synovial membrane of the joints and artificial lymph nodes. Accordingly, readers will learn that ILO structure and function can vary substantially, depending on the context. Presenting the results of the latest immunological research, the book offers a fascinating and insightful read for both scientists and clinicians in the areas of infectious and immune-associated diseases.

This book is a comprehensive resource on vaccines and immunizations for primary care physicians, advanced practice providers, and trainees. We are now seeing a rise in measles and the potential for rises in other previously rare infectious diseases, significantly due to public and physician misconceptions and misinformation about vaccines. The text addresses this issue by consolidating historical and current advances in vaccine science from how vaccines are developed to CDC recommendations on how and when to administer them. Expert authors also address barriers to improving vaccination rates in the U.S. and offer evidence-based recommendations on overcoming those barriers. This is an essential guide for primary care physicians, family physicians, pediatricians, internists, residents, medical students, mid-level providers, and learners for understanding vaccines and improving preventative care for their patients.

This book describes the newest developments in antibody drug conjugates and immunotoxins, paving their way to clinical application. Lessons learned from the current state of the art are used to further improve our understanding of their mechanisms of action and off target activities. The book introduces scientists to all of the prerequisites that must be properly addressed, including identification of the right target, specific traits of target binding antibodies, proper selection of the toxic payload, internalization induced by binding, and next generation conjugation and linker technologies. These knowledge-based, revolutionary new drug principles will form the cornerstone of the future standard of care and will lead to major advances in application, as well as improved quality of life and patient survival rates. This book will be of interest to biotech companies and researchers working in the fields of immunology, pharmacology, and oncology.

This book highlights information derived primarily from clinical samples, with particular reference to theoretical and scientific aspects of the human immune system. This text will focus on topics that range from host-pathogen interactions in infectious disease to host immune response in cancer, allergic diseases, neuroinflammatory diseases, and autoimmune disorders. The reader will also have a well-rounded understanding of the behavior of the immune system with particular emphasis on the role of immunoproteomics in immunotherapy, neuroprotective immunity for neurodegenerative and neuroinfectious disease, leukemia-associated dendritic cell induction of adaptive immunity dysregulation, and the role of immune checkpoint inhibitors in cancer, infection, as well as neuroinflammation. Taken together, the contents of this book are intended for both clinicians and researchers in academia and industry.

This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors, electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.

Glycobiology: The Basics: Novel Pathways in Complex-Type Oligosaccharide Synthesis: New Vistas Opened by Studies in Invertebrates; D.H. Van den Eijnden, et al. Defective Glycosyltransferases Are Not Good for Your Health; H. Schachter, et al. Probing CarbohydrateProtein Interactions by HighResolution NMR Spectroscopy; S.W. Homans, et al. Oligosaccharides and Protein Recognition: The Structure of a Human Rheumatoid Factor Bound to IgG Fc; B.J. Sutton, et al. Carbohydrate Recognition Systems in Innate Immunity; T. Feizi. Biosynthesis of Sulfated LSelectin Ligands in Human High Endothelial Venules (HEV); J.P. Girard, F. Amalric. Endothelial Sialyl Lewis x as a Crucial Glycan Decoration on LSelectin Ligands; R. Renkonen. Role of LectinGlycoconjugate Recognitions in CellCell Interactions Leading to Tissue Invasion; C. Kieda. Oligosaccharides and Biological Function: Protein OGlcNAcylation: Potential Mechanisms for the Regulation of Protein Function; B.K. Hayes, G.W. Hart. A Longitudinal Study of Glycosylation of a Human IgG3 Paraprotein in a Patient with Multiple Myeloma; M. Farooq, et al. The Role of The Lectin Calnexin in Conformation Independent Binding to NLinked Glycoproteins and Quality Control; J.J.M. Bergeron, et al. Glycosylation and Inflammation: Immunodetection of Glycosyltransferases: Prospects and Pitfalls; E.G. Berger, et al. Cytokine and Protease Glycosylation as a Regulatory Mechanism in Inflammation and Autoimmunity; P. Van den Steen, et al. Occurrence and Possible Function of InflammationInduced Expression of Sialyl Lewis-X on Acute-Phase Proteins; W. Van Dijk, et al. Glycosylation and Disease: The Glycosylation of the Complement Regulatory Protein, Human Erythrocyte CD59; P.M. Rudd, et al. Glycosylation and Rheumatic Disease; J.S. Axford. IgA Glycosylation in IgA Nephropathy; A. Allen, J. Feehally. Oligosaccharide Profiling of Acute-Phase Proteins: A Possible Strategy towards Better Markers in Disease; G.A. Turner, M.T. Goodarzi. The Role of NLinked Glycosylation in the Secretion of Hepatitis B Virus; A. Mehta, et al. Role of Glycan Processing in Hepatitis B Virus Envelope Protein Trafficking; T.M. Block, et al. Glycotherapeutics: Combinatorial Carbohydrate Chemistry; Z.G. Wang, O. Hindsgaul. Bacterial Lipopolysaccharides: Candidate Vaccines to Prevent Neisseria meningitidis and Haemophilus influenzae Infections; E.R. Moxon, et al. Development of Double Copy Dicistronic Retroviral Vectors for Transfer and Expression of Glycosyltransferase Genes; D. Izycki, et al. Oligosaccharide Epitope Diversity and Therapeutic Potential; E.F. Hounsell, D.V. Renouf. The Group B Streptococcal Capsular Carbohydrate: Immune Response and Molecular Mimicry; R.G. Feldman, et al. Index.

This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Molecular Typing of Blood Cell Antigens summarizes contributions from leading scientist in the field DNA typing for blood cell antigens.

Insights into the regulation of immune cell lineage differentiation and specification as well as into the control of lineage integrity, stability and plasticity are of fundamental importance to understanding innate and adaptive immune responses. In this volume, leading experts provide an up-to-date and comprehensive overview of recent advances in the transcriptional control mechanisms and transcription factor networks that regulate these processes in a variety of different immune cell lineages. The chapters cover the regulation of T versus B cell lineage choice, discuss early B cell development and pre-B cell leukemia prevention, address transcriptional control mechanisms during the differentiation, in regulatory T cells and iNKT cells, detail genomic switches in helper cell fate choice and plasticity and highlight the role of the BTB-zinc finger family of transcription factors in T cells. Moreover, the chapters discuss transcriptional networks in DCs, NK cells and in innate lymphoid cells. Together, the reviews illustrate key transcriptional control mechanisms that regulate the development and function of immune cells and demonstrate the impressive advances made over the last decade.

This issue of Immunology and Allergy Clinics, devoted to Obesity and Asthma, is edited by Dr. Anurag Agrawal. Articles in this issue include: Clinical Implications of the Obese Asthma Phenotypes; Childhood Obesity and the Risk of Allergy; Metabolic Asthma: Is there a link between obesity, asthma, and diabetes?; Role of Weight Management in Obese-Asthma Control; Obesity and Airway Disease: A Bioenergetic Problem?; Nutrition, Obesity, and Asthma: Exploring Epigenetic Programming; Obesity and Asthma: The Role of Environmental Pollutants; Novel Therapeutic Strategies for Adult Obese Asthmatics; and From ADMA to Asthma.

Autophagy principally serves an adaptive function to protect organisms against diverse human pathologies, including cancer and neurodegeneration. Recent developments using in vitro, ex vivo and in vivo models show the involvement of the autophagy pathway in immunity and inflammation. Moreover, direct interactions between autophagy proteins and immune signalling molecules have also been demonstrated. Defects in autophagy - similar to cancer, neurodegenerative diseases and aging - through autophagy gene mutation and/or microbial antagonism, may underlie the pathogenesis of many infectious diseases and inflammatory syndromes. In spite of the increasing awareness of the importance of autophagy in these pathophysiological conditions, this process remains underestimated and is often overlooked. As a consequence, its role in the initiation, stability, maintenance, and progression of these diseases are still poorly understood. This book reviews the recent advances regarding the functions of the autophagy pathway and autophagy proteins in immunity and inflammation, focusing on their role in self-nonself distinction, their implications in innate and adaptive immune responses and their dysregulation in the pathology of certain inflammatory and autoimmune diseases.

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Primary Immunodeficiency Disorders: A Historic and Scientific Perspective "provides a complete historical context that is crucial for students and researchers concerned with primary immunodeficiency. When researchers have a poor understanding of the way we arrived where we are in research, they can miss important points about a disease, or miss out on how to approach new diseases. This historical knowledge of research can assist greatly by showing how it was done in the past, demonstrating the successes and failures, so that it can be done better in the future.

This book provides an understanding of the process going from clinical problem to lab and back to the clinic, based on historical experiences. Its chapters proceed from the discovery of the T and B cell lineages through the first BMT for immunodeficiency disorder; lab investigation and gene therapy for PID; the discovery of the gene for AT and its function; understanding cytokine defects; and many other stops along the way.
Facilitates communication among physicians and other investigators concerned with immunological and inflammatory diseasesSummarizes for the first time all the known facts from 60 years of primary immunodeficiency research, and teaches how an important field in medicine was establishedProvides stimulating discussions on developing new medical therapies
Highlights the importance of studying humans to understand mechanisms of disease that affect humans

"Natural Hosts of SIV: Implications in AIDS" thoroughly reviews the possible mechanisms by which African nonhuman primate natural hosts of lentiviruses remain essentially disease-free while other hosts exhibit disease and death. The book ultimately indicates directions for further research and potential translations of this compelling phenomenon into novel approaches to treat and prevent HIV. When Asian non-human primate non-natural hosts are experimentally infected with viruses isolated from African species, disease and death normally results. Meanwhile, these African nonhuman primate natural hosts maintain similar levels of plasma and cellular viremia and exhibit compellingly different, essentially disease-free, states. This work attempts to answer the question of how the natural host remains disease resistant.
Summarizes the past 30 years of research in this field and describes the latest developments in AIDS research using nonhuman primate animal modelsProvides insights into how this large body of scientific work can be translated into novel approaches to treat and prevent HIVHighlights the areas that merit future pursuit, focusing on potential applications for the treatment and prevention of HIV infection

This issue of Immunology and Allergy Clinics, edited by Dr. Pascal Demoly, is devoted to Drug Hypersensitivity.? Articles in this issue include: Drug allergy diagnosis; Principles of drug allergy management: acute drug reactions, individual preventive measures, desensitization, general preventive measures; Antibiotics allergy; Non-steroidal anti-inflammatory drugs HS; Local and general anesthetics allergy; Radiocontrast media and dyes allergy; Chemotherapeutic agents allergy; Vaccine allergy; Biological agents HS; Anti-epileptic allergy; Iron and Vitamin Allergy; Antiviral drug allergy; Additive allergy; and Unmet needs in drug allergy.

"Celiac Disease and Gluten: Multidisciplinary Challenges and Opportunities" is a unique reference work the first to integrate the insights of the causes and effects of celiac disease from the chemistry of reaction-causing foods to the diagnosis, pathogenesis, and symptoms that lead to proper diagnoses and treatment. With an estimated three million people in the United States alone affected by celiac disease, an autoimmune digestive disease, onlyfive percentare properly diagnosed. Drawing on the connection between foods containing gluten and the resulting symptoms, this resource offers distinctive information that directly explores and links food science, medical diagnostics, and treatment information. A helpful tool for researchers and medical practitioners alike, "Celiac Disease and Gluten: Multidisciplinary Challenges and Opportunities" helps refine research targets, and provides a comprehensive overview on the multidisciplinary approaches to all crucial aspects related to celiac disease.
Presents key information from medical and food science research, as well as provides clinical insightsProvides direct corollary insights between source and symptomWritten by experts whose detailed experiments and results have shaped our understanding of celiac disease"

"Advances in Immunology, " a long-established and highly respected publication, presents current developments as well as comprehensive reviews in immunology. Articles address the wide range of topics that comprise immunology, including molecular and cellular activation mechanisms, phylogeny and molecular evolution, and clinical modalities. Edited and authored by the foremost scientists in the field, each volume provides up-to-date information and directions for the future.

Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field