The Natural Anti-Gal Antibody as Foe Turned Friend in Medicine provides a comprehensive review of the natural anti-Gal antibody, which is the most abundant antibody in humans constituting ~1% of immunoglobulins and the carbohydrate antigen it recognizes, the a-gal epitope. It discusses the discovery of this antigen/antibody system, its evolution in mammals, the pathological effects of this antibody, and its possible use in various therapies in humans. Most significantly, the book discusses microbial and regenerative therapies in which an antibody present in all humans may be harnessed as an in vivo pharmaceutical agent that enables a wide variety of therapies. Some of these therapies are described as experimental studies that are compiled in this book, other already studied therapies in the area of cancer immunotherapy are also included in this book.

Advances in Microbial Physiology, Volume 71, continues the long tradition of topical, important, cutting-edge reviews in microbiology. The book contains updates in the field, with comprehensive chapters covering The Microbiology of Ruthenium Complexes, The role of plant growth-promoting bacteria in metal phytoremediation, the Mechanism and Role of Globin Coupled Sensor Signaling, Cytochrome bd and gaseous ligands in bacterial physiology, and Haem-Based Sensors of O2: Lessons and Perspectives.

Zebrafish at the Interface of Development and Disease Research presents the latest on the shared pathways that govern development and contribute to disease. Zebrafish have traditionally been used to study vertebrate development, providing interesting data on the developmental processes and genes that are implicated in disease. This new release in the series contains informative discussions on congenital heart defects in zebrafish, the use of zebrafish in studying kidney development and disease, and muscle development, homeostasis and disease in zebrafish. Each chapter interweaves the study of zebrafish development and its application to the immune system, the kidney, liver, heart and others.

Systemic lupus erythematosus (SLE or lupus) is a fairly common disease with a relatively high mortality rate that is characterized by multisystemic disorders, including the kidney. Recently, epidemiologic studies also show that the incidence of new SLE cases is increasing. Renal involvement is very common in systemic lupus erythematosus, occurring in up to 2/3 of patients and is a major cause of morbidity and mortality. Lupus nephritis affects glomeruli, tubules, interstitium peritubular capillaries, arteries, and veins with a spectrum of lesion. Although tubular, interstitial, and vascular lesions are common in lupus nephritis and may contribute significantly to overall disease severity, activity, and chronicity, they are not considered in most important classifications (OMS and ISN/RPS). These classifications are based entirely on the evaluation of glomerular alterations. Furthermore, the increasingly recognized, new pathological entity called lupus podocitopathy in the context of SLE, is not also accepted in the above classifications. Amyloidosis, non-lupus nephritides (including IgA nephropathy), therapeutic nephrotoxicity, and lupus in overlap with other connective tissue diseases are occasionally described. Therefore, this new case-based book fills a gap in the literature, and the authors call attention to some clinical entities which are less known and more difficult to recognize. The book design allows correlating morphologic aspects with clinical findings, and determines the prognosis and therapeutic response. It is a useful resource for pathologists, nephrologists, rheumatologist, physicians, and lupus and kidney students. Each case consists of typical histology images, a brief history, diagnosis, discussion, and pearls and pitfalls. The book is based on a multicenter experience, and the importance of kidney biopsy in the differential diagnosis is emphasised.

Eat as much as you want, whenever you want. Welcome to the end of dieting.

We're fatter, sicker, and hungrier than ever, and the billion-dollar diet industry--with its trendy weight-loss protocols and eat-this-notthat ratios of fat, carbs, and protein--offers only temporary short-term solutions at the expense of our permanent long-term health. As a result, we're trapped in a cycle of food addiction, toxic hunger, and overeating.

In The End of Dieting, Joel Fuhrman, M.D., a board-certified doctor and the New York Times bestselling author of Eat to Live and The End of Diabetes, shows us how to break free from this vicious cycle once and for all. Dr. Fuhrman lays out in full all the dietary and nutritional advice necessary to eat our way to a healthier and happier life. At the center of his revolutionary plan is his trademark health formula: Health = Nutrients/Calories. Foods high in nutrient density, according to Dr. Fuhrman, are more satisfying than foods high in calories. They eliminate our cravings for fat, sweets, and carbs. The more nutrient-dense food we consume, the more our bodies can function as the self-healing machines they're designed to be. Weight drops, diseases reverse course and disappear, and our lives become longer and healthier.

The End of Dieting is the book we have been waiting for--a proven, effective, and sustainable approach to eating that lets us prevent and reverse disease, lose weight, and reclaim our right to excellent health.

This issue of Immunology and Allergy Clinics, guest edited by Drs. Linda S. Cox and Anna H. Nowak-Wegrzyn, is devoted to Aeroallergen and Food Immunotherapy. Articles in this issue include History of AIT and the future direction of CRD/molecular allergy; Mechanisms of aeroallergen allergen immunotherapy: SCIT and SLIT; Mechanisms underlying induction of tolerance to foods; Biomarkers for allergen immunotherapy; SCIT and SLIT; Novel delivery routes for allergy immunotherapy; Oral immunotherapy for food allergy; SLIT and EPIT for food allergy; The use of adjuvants for enhancing allergen immunotherapy efficacy; Allergen immunotherapy vaccine modification; Allergen immunotherapy outcome assessment in clinical trials and real life; Component resolved diagnosis: can it make specific AIT more specific?; Baked milk and egg diets for milk and egg allergy management; and Allergen immunotherapy practical considerations: adherence and strategies to improve.

Immunology: A Foundation Text This book provides an integrated approach to the study of immunology for people who seek a user-friendly', concise and up-to-date foundation text. There can be few subjects that so obviously demand such an approach. Immunology overlaps with the biological disciplines of biochemistry, cell biology, genetics, physiology, microbiology and parasitology - relying on methods and concepts derived from these disciplines, and in turn contributing to them. After describing the components of the immune system, and explaining how they interact, the author goes on to discuss the many important applications of immunology in laboratory techniques and in medicine. In addition to this introductory' material, the book considers new, and likely future developments - giving the reader a taste of the rapid rate of progress in this important field. The author's clear and concise text is supported by a wealth of illustrations (most in two-colour), self-assessment questions and a comprehensive index - making this an essential text for all life science students entering this field, or for health practitioners seeking to update their knowledge.

Behcet's disease is a chronic relapsing inflammatory disease of unknown etiology involving multiple organs. Along with blindness, other bodily functions are greatly affected by this disease; conditions of the vascular, intestinal and central nervous systems are usually life threatening and require aggressive therapy. Recently, significant progress has been made in several areas of this disease. The introduction of biological agents (such as anti-TNF) have had a positive impact on the effectiveness of treatments. However, there are still a number of unmet needs in various fields of this disease. Despite this, there remains a number of unaddressed issues concerning the treatments of this disease. Several genes have been shown to be associated with this disease, in addition to HLA-B51. Abnormalities in several subsets of T helper cells have been implicated in the pathogenesis of Behcet's disease. Recent advances in the field of innate immunity have shed light on the new aspects of Behcet's disease; that is, this disease has both autoimmunity and autoinflammatory aspects. On the other hand, there are still controversies as to the diagnosis and treatment of recalcitrant manifestations of the disease. This publication highlights the most recent understanding of Behcet's disease and raises several unanswered questions in both basic to clinical treatment of this ailment. The contents of this book are primarily based on the most up-to-date results of research programs sponsored by the Japanese government as well as findings from Korean researchers. In particular, novel classification for neurological involvement (which has been discovered in Japan) led to the establishment of diagnostic criteria and treatment recommendation. Thus, this publication brings forth useful information for a variety of specialists who are involved in the management of this intractable disease.

Translational Immunology: Mechanisms and Pharmacologic Approaches highlights and summarizes the most important advances in human immunology, clinical translations, new tools to analyze therapeutic targets, and new pharmacological approaches for autoimmunity, inflammatory disorders, and cancer. The book is an essential resource for those seeking to understand the potential translational applications of burgeoning studies in human immunology, helping readers make sense of the existing and emerging scientific advances. The book grounds fundamental science in the translational realm, providing insights from world renowned researchers at the top of their game in their respective fields, in both industry and academic settings. Readers will gain an understanding of the rationale and mechanisms underlying current and emerging pharmacologic approaches for interventional immunology, the gaps therein, and new ideas for better and safer therapeutic approaches, and physicians will glean information about pharmacological limitations in altering disease progression and complications. This reference on the translational realization of the burgeoning findings in immunology provides a go-to reference for experienced professional clinicians, researchers, industry scientists, and those seeking more information on the field.

Autoimmune neurologic disease results when the immune response to the body s own organs run wild and causes the immune system to attack itself. Autoimmunity is a main element in numerous nervous system diseases and can target any structure within the central or peripheral nervous system. Over the past 20 years significant advances in our understanding of the pathophysiology of autoimmune disorders including biomarkers has led to new diagnosis and treatment options. Neurologic conditions associated with autoimmune reactions include, dementia, neuromuscular disease, epilepsy, sleep disorders, diabetes, and other common neurologic disorders and disease. This current tutorial-reference will be a must-have title for clinical neurologists, research neurologists and neuroscientists, and any medical professional working with autoimmune disease and disorders.

*Comprehensive coverage of autoimmune neurology *Details the latest techniques for the study, diagnosis and treatment of diseases and disorders including dementia, neuromuscular disease, epilepsy, sleep disorders *A focused reference for clinical practitioners and the clinical neurology and neurology research communities"

Food allergies have a considerable impact on modern society. Some of the most severe allergic reactions occur when peanuts and peanut derivatives are consumed. The main causative agents of a peanut allergy are proteins. The total protein content of a peanut is represented by thirty-two different proteins, of which about eighteen (nearly 7-10%) have been identified as capable of binding specific IgE, and so can be considered allergenic. Comprised of six chapters, this book comprehensively covers all topics of peanut allergy. A unique, concise and up-to-date resource, it offers readers an innovative and valuable presentation of the subject. It has been carefully prepared to present the concepts with the help of diagrams and tables. Each chapter is presented in a simple and systematic way to provide a thorough understanding of the core aspects of peanut allergy and the components of peanuts which cause allergic reactions. The basic concepts of clinical aspects, peanut allergens properties, cross reactivity and stability of peanut allergens and the legislation to protect sensitive people are clearly explained. Recent discoveries of peanut allergens are highlighted. From the description of the first peanut allergen called Ara h1 in 1991, so far there have been about thirteen proteins with allergenic action described. Theallergenic proteins in a peanut, named Ara h1 Ara h17 and agglutinin have been identified, largely characterised and accepted by the Allergen Nomenclature Subcommittee of the International Union of Immunological Societies (IUIS). Some of these allergens have established structural features, biochemical and physicochemical properties and their biosynthesis genes encoded. Being proteins, peanut allergens can suffer some modifications during food processing and digestion (acylation, polymerisation, nitration, Maillard reaction etc.), and interactions within complex food matrices (both natural and fabricated structures). These modifications can reduce or increase their allergenic properties and are influenced by the food matrix. Peanut allergens have different cross-reactions with other allergens such as, for example, those of soybean, peas, lima beans, green beans, chickpeas, lentils or other beans. Methods commonly used to detect and quantify peanut allergens are mainly immunochemical and molecular methods. However, methods have been developed based on HPLC techniques coupled both with and without mass spectrometry, capillary electrophoresis, circular dichroism, FTIR or NMR. Peanut allergy is high and there is currently no treatment for it. There is a lethal risk associated with peanut allergy, and in order to protect public health, the regulatory bodies worldwide will issue legislation concerning a requirement to clearly have a warning label of the possible presence of food allergens. The last chapter contains some of the most used preventive actions and elaborated legislation in different countries.

Cancer glycobiology is a blazing hot area of cancer research with aberrant post-translational glycosylations on lipids and proteins considered critical for cancer development and metastasis. The NIH/NCI Alliance of Glycobiologists for Cancer Research is a consortium of (10) tumor glycobiology laboratories funded to investigate how cancer-associated glycans (CAG) impact the development and metastasis of cancer. The hope of this momentous effort is to discover CAGs for use as novel biomarkers to detect early-stage cancer or its aggressive behavior or, alternatively, as novel molecular targets with the promise of a curative approach to cancer therapy. The mission of the 'Alliance' is to study structure and function of CAGs in cancer development and to provide clinically useful biomarkers for detecting cancer early or the potential of metastasis. This charge has stimulated extensive collaborations across US and International institutions to accelerate discovery of glycan-based biomarkers to the forefront of NCI's efforts to diagnose early-stage cancer and provide curative anti-cancer therapies. The main goals of these research efforts are: 1. To identify and study how cancer-associated glycans functionally impact cancer growth and metastasis 2. To identify cancer-associated glycans that can serve as biomarkers for the detection or progression of cancer 3. To develop cancer-associated glycans as novel therapeutic targets for the treatment and cure of cancer This volume consists of (9) chapters, one from each funded laboratory, that reviews new methods and mechanisms highlighting glycoconjugates and their roles as cancer biomarkers and/or anti-cancer therapeutic targets.

Personalized Immunosuppression in Transplantation: Role of Biomarker Monitoring and Therapeutic Drug Monitoring provides coverage of the various approaches to monitoring immunosuppressants in transplant patients, including the most recently developed biomarker monitoring methods, pharmacogenomics approaches, and traditional therapeutic drug monitoring. The book is written for pathologists, toxicologists, and transplant surgeons who are involved in the management of transplant patients, offering them in-depth coverage of the management of immunosuppressant therapy in transplant patients with the goal of maximum benefit from drug therapy and minimal risk of drug toxicity. This book also provides practical guidelines for managing immunosuppressant therapy, including the therapeutic ranges of various immunosuppressants, the pitfalls of methodologies used for determination of these immunosuppressants in whole blood or plasma, appropriate pharmacogenomics testing for organ transplant recipients, and when biomarker monitoring could be helpful.

This issue of Hematology/Oncology Clinics of North America, devoted to Colorectal Cancer, is edited by Dr. Leonard B. Saltz. Articles in this issue include: Diet and Lifestyle Influences on Colorectal Cancer; Diagnosis and Management of Germline and Somatic Mutations in Mismatch Repair-deficient Colorectal Cancer; Adjuvant Chemotherapy in Stage II, III, and Resected Stage IV Colon Cancer; Predictive and Prognostic Markers in the Treatment of Colorectal Cancer; Surgical Management of Hepatic Metastases of Colorectal Cancer; Combination Therapies in Colorectal Cancer Treatment; A Critical Look at Management of Peritoneal Metastases of Colorectal Cancer; Non-surgical Management of Rectal Cancer; and Ablative Therapies for Metastatic Colorectal Cancer.

Autoimmunity, COVID-19, Post-COVID-19 Syndrome and COVID-19 Vaccination covers all aspects of what is perhaps the most dramatic health crisis in the history of modern medicine. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) raised major concerns about the association between the virus and various autoimmune manifestations. Over 15 distinct autoantibodies and above 10 different autoimmune diseases were found to develop in COVID-19 patients. Moreover, evidence about recovered COVID-19 patients demonstrates that persistent systemic symptoms, which are believed to have an autoimmune-related mechanism, do exist. As it is of great importance to recognize those autoimmune manifestations of COVID-19 and post-COVID-19 syndrome to properly cope with their outcomes in the ongoing pandemic and the long-term post-pandemic period, this book fulfills a vital need in the medical community.

Sarcoidosis is a multisystem granulomatous disease of unknown etiology. It can occur at all ages, with the incidence peaking at 20 to 40 years. This book reviews the epidemiology, etiology, pathology, clinical manifestations, diagnosis and management strategies of sarcoidosis, while highlighting recent studies that have provided new insights into this unique and debilitating disease. The authors also specifically point out the urological manifestations of sarcoidosis to avoid misdiagnoses and to provide early intervention.

T Lymphocyte Subpopulations in Immunotoxicology Edited by Ian Kimber ZENECA Pharmaceuticals, Central Toxicology Laboratory, Macclesfield, Cheshire, UK and Mary Jane K. Selgrade US Environmental Protection Agency, National Health and Environmental Effects Research Laboratory, NC, USA Immunotoxicology is a diverse discipline that embraces the investigation of the adverse health effects that may result from the interaction of xenobiotics with the immune system. T lymphocytes orchestrate adaptive immune responses and are of central importance in the consideration of immunotoxicity. These cells and/or the tissue responsible for their functional maturation (the thymus) have been implicated as the primary or secondary targets for chemical insult. Moreover, T lymphocytes play pivotal roles in the induction and elicitation of chemical and protein allergy and in the pathogenesis of autoimmune disease. Classically, T lymphocytes have been divided into two main populations: CD4 helper T cells and CD8 suppressor/cytotoxic T cells. It is now apparent that there exists within both populations considerable functional heterogeneity, characterized usually by variable cytokine secretion patterns. Two major subpopulations of T helper (Th) cells have been identified and designated Th1 and Th2, with similar, but less well characterized, sub-types of cytotoxic (Tc) cells having been described also. The discovery of functional subpopulations of Th (and Tc) cells has profoundly influenced our view of the immune response and, in particular, our appreciation of the way in which the immune system can tailor responses to meet challenges of different types. This has also translated into a much more detailed understanding of certain immunopathologic processes and the role of T lymphocytes in the development of allergic, autoimmune and inflammatory diseases. This book explores the role of T lymphocyte subpopulations in the development and expression of immunotoxic effects. Constituent chapters reveal that a consideration of the impact of chemicals on these diverse T cell populations and the polarization of T cell responses has already contributed greatly to our understanding of the mechanisms underlying immunotoxicity, including immune suppression and allergic and autoimmune responses.

Developing Costimulatory Molecules for Immunotherapy of Diseases highlights the novel concept of reverse costimulation and how it can be effectively exploited to develop immunotherapy using either humanized antibodies against CD80, CD86, and other costimulatory molecules or CD28 fusinogenic proteins in the treatment of diseases, including allergies, asthma, rheumatoid arthritis, multiple sclerosis, lupus nephritis, severe psoriasis, vulgaris tuberculosis, thopoid, transplantation therapeutic, cancer, and inflammation. The text aims to provide the latest information on the complex roles and interactions within the CD28 and B7 costimulatory families, with the hope that targeting these families will yield new therapies for the treatment of inflammation, autoimmunity, transplantation, cancer, and other infectious diseases.

'In an era of heightened awareness of our immune system, the threat of pandemics, the rise of autoimmunity, and the revelatory new science that everything from heart disease to cancer, Alzheimer's to autism, and depression to diabetes is linked to disordered immune function and inflammation, The Immunotype Breakthrough is the most important guide available today to understand and optimise our immune function. It is an essential roadmap for anyone with an immune system (essentially everyone!), anyone looking to optimise their health and avoid today's modern plague - inflammation.' DR MARK HYMAN, New York Times-bestselling author of The Pegan Diet. 'The immune system has been overlooked and misunderstood for far too long. In The Immunotype Breakthrough, Dr Moday gives us a glimpse into her speciality and explains the recent research in a way that we can all understand. Best of all, she gives us action steps we can each take to prioritise our immune system to elevate our overall health.' DR WILL COLE, New York Times-bestselling author of Intuitive Fasting 'This is a must-read for anyone who has experienced the pitfalls of conventional medicine and is ready to balance their immune system for optimal health.' DR AMY MYERS, author of The Autoimmune Solution and Thyroid Connection 'A revolutionary book from one of the best immunity experts around. The Immunotype Breakthrough explains the latest science on immunity, and offers unique, customisable advice to help you balance your immune system. A must-read for anyone interested in living a long and healthy life." JASON WACHOB, Founder & Co-CEO of mindbodygreen.com To most of us, the immune system is a mysterious and complex network of cells, receptors, and messengers that we only notice when it goes awry. But our immune response, or lack of, affects nearly every aspect of our bodies and our health. Much has been made of 'boosting' immunity, but what exactly does that mean? And what if boosting isn't really what our immune systems need? In The Immunotype Breakthrough, functional medicine doctor and immunity specialist Dr Heather Moday explains that, for the most part, what our immune systems truly need is not boosting, but balancing. She identifies four distinct immunotypes, and helps you find yours: Smouldering: too much inflammation, resulting in diabetes, dementia, heart disease Misguided: an immune system which attacks your own cells and organs, causing autoimmune diseases Hyperactive: overreactive to harmless substances, causing allergies Weak: an immune system that doesn't react fast or effectively enough, causing frequent colds, flu or bronchitis Drawing on a wealth of cutting-edge research, Dr Moday explains that our immune systems are fluid and significantly influenced by our behaviours, diet, habits, and environment. By identifying your personal immunotype, and implementing Dr Moday's Immune Restoration Plan, you can make focused, individualised lifestyle changes to bring your immune system back into balance. With interactive quizzes to help readers zero in on their own specific needs, The Immunotype Breakthrough is a revolutionary programme that will lead to holistic health, vitality, and longevity for everyone.

"Natural Hosts of SIV: Implications in AIDS" thoroughly reviews the possible mechanisms by which African nonhuman primate natural hosts of lentiviruses remain essentially disease-free while other hosts exhibit disease and death. The book ultimately indicates directions for further research and potential translations of this compelling phenomenon into novel approaches to treat and prevent HIV. When Asian non-human primate non-natural hosts are experimentally infected with viruses isolated from African species, disease and death normally results. Meanwhile, these African nonhuman primate natural hosts maintain similar levels of plasma and cellular viremia and exhibit compellingly different, essentially disease-free, states. This work attempts to answer the question of how the natural host remains disease resistant.
Summarizes the past 30 years of research in this field and describes the latest developments in AIDS research using nonhuman primate animal modelsProvides insights into how this large body of scientific work can be translated into novel approaches to treat and prevent HIVHighlights the areas that merit future pursuit, focusing on potential applications for the treatment and prevention of HIV infection

"Celiac Disease and Gluten: Multidisciplinary Challenges and Opportunities" is a unique reference work the first to integrate the insights of the causes and effects of celiac disease from the chemistry of reaction-causing foods to the diagnosis, pathogenesis, and symptoms that lead to proper diagnoses and treatment. With an estimated three million people in the United States alone affected by celiac disease, an autoimmune digestive disease, onlyfive percentare properly diagnosed. Drawing on the connection between foods containing gluten and the resulting symptoms, this resource offers distinctive information that directly explores and links food science, medical diagnostics, and treatment information. A helpful tool for researchers and medical practitioners alike, "Celiac Disease and Gluten: Multidisciplinary Challenges and Opportunities" helps refine research targets, and provides a comprehensive overview on the multidisciplinary approaches to all crucial aspects related to celiac disease.
Presents key information from medical and food science research, as well as provides clinical insightsProvides direct corollary insights between source and symptomWritten by experts whose detailed experiments and results have shaped our understanding of celiac disease"

This reference book compiles together different animal models in cancer research. It provides knowledge and a better understanding of the advancement of the molecular and cellular mechanisms associated with the progression, formation, and clinical results of various types of cancer from the evidence collected from animal models utilized for cancer research. It discusses animal models for screening anti-cancer drugs and exploration of gene therapy. It presents different methods used to construct cancer animal models and the progress of each animal model in tumor research. The book also highlights the applications of genetic engineering, including CRISP/Cas9, in designing and developing animal models for cancer research. Further, it discusses strategies for modeling animals for investigating growth, metastasis, tumor-associated inflammation and microenvironment, cancer stem cells, tumor heterogeneity, and therapeutic resistance. This book is s a valuable resource for basic and translational cancer researchers, clinicians, and health care.

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Primary Immunodeficiency Disorders: A Historic and Scientific Perspective "provides a complete historical context that is crucial for students and researchers concerned with primary immunodeficiency. When researchers have a poor understanding of the way we arrived where we are in research, they can miss important points about a disease, or miss out on how to approach new diseases. This historical knowledge of research can assist greatly by showing how it was done in the past, demonstrating the successes and failures, so that it can be done better in the future.

This book provides an understanding of the process going from clinical problem to lab and back to the clinic, based on historical experiences. Its chapters proceed from the discovery of the T and B cell lineages through the first BMT for immunodeficiency disorder; lab investigation and gene therapy for PID; the discovery of the gene for AT and its function; understanding cytokine defects; and many other stops along the way.
Facilitates communication among physicians and other investigators concerned with immunological and inflammatory diseasesSummarizes for the first time all the known facts from 60 years of primary immunodeficiency research, and teaches how an important field in medicine was establishedProvides stimulating discussions on developing new medical therapies
Highlights the importance of studying humans to understand mechanisms of disease that affect humans

A search for a missing son... and a toxic mystery that threatens the globe.

'Did a 1981 Dean Koontz thriller predict the coronavirus outbreak?' Daily Mail
'Dean Koontz is not just a master of our darkest dreams, but also a literary juggler' The Times

From bestselling phenomenon Dean Koontz, The Eyes of Darkness is a gripping thriller following a mother's search for her son - a journey that unlocks the deadliest of secrets.

It's a year since Tina Evans lost her little boy Danny in a tragic accident.
Then a shattering message appears on the blackboard in Danny's old room: NOT DEAD.
Is it someone's idea of a grim joke? Or something far more sinister?

The search for an answer drives Tina through the neon clamour of Las Vegas nightlife.
The sun-scorched desert. The frozen mountains of the High Sierra.

People face a dreadful danger as a buried truth struggles to surface.
A truth so frightening that its secret must be kept at the price of any life - any man, any woman...any child.