Cell Imaging is rapidly evolving as new technologies and new imaging advances continue to be introduced. In the second edition of Cell Imaging Techniques: Methods and Protocols expands upon the previous editions with current techniques that includes confocal microscopy, transmission electron microscopy, atomic force microscopy, and laser microdissection. With new chapters covering colocalization analysis of fluorescent probes, correlative light and electron microscopy, environmental scanning electron microscopy, light sheet microscopy, intravital microscopy, high throughput microscopy, and stereological techniques. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and cutting-edge, Cell Imaging Techniques: Methods and Protocols, Second Edition is an easily accessible volume of protocols to be used with a variety of imaging-based equipment likely available in a core imaging facility.

Developments in teleradiology are progressing at great speed. As a consequence, there is a need for a broad overview of the field. This first-ever book on teleradiology is presented in such a way that it should make it accessible to anyone, independent of their knowledge of technology. The text is designed to be used by all professionals, including radiologists, surgeons, nurses and allied health professionals, and computer scientists. In a very short time, driven by technical developments, the field of teleradiology has become too extensive to be covered by only a small number of experts. Therefore, Teleradiology has been written with chapter contributions from a host of renowned international authorities in teleradiology (see the Contents and the Contributors). This ensures that the subject matter focusing on recent advances in teleradiology is truly up to date. Our guiding hope during this task was that as editors of multiple chapters we could still write with a single voice and keep the content coherent and simple. We hope that the clarity of this book makes up for any limitations in its comp- hensiveness.

Based on the author's lecture notes and research, this well-illustrated and comprehensive text is one of the first to provide an introduction to image registration with particular emphasis on numerical methods in medical imaging. Ideal for researchers in industry and academia, it is also a suitable study guide for graduate mathematicians, computer scientists, engineers, medical physicists, and radiologists. Image registration is utilised whenever information obtained from different viewpoints needs to be combined or compared and unwanted distortion needs to be eliminated. For example, CCTV images, ultrasound images, brain scan images, fingerprint and retinal scanning. Modersitzki's book provides a systematic introduction to the theoretical, practical, and numerical aspects of image registration, with special emphasis on medical applications. Various techniques are described, discussed and compared using numerous illustrations. The text starts with an introduction to the mathematical principles and the motivating example of the Human Neuroscanning Project whose aim is to build an atlas of the human brain through reconstructing essential information out of deformed images of sections of a prepared brain. The introduction is followed by coverage of parametric image registrations such as landmark based, principal axes based, and optimal affine linear registration. Basic distance measures like sum of squared differences, correlation, and mutual information are also discussed. The next section is devoted to state-of-the-art non-parametric image registrations where general variational based framework for image registration is presented and used to describe and compare well-known and new image registration techniques. Finally, efficient numerical schemes for the underlying partial differential equations are presented and discussed. This text treats the basic mathematical principles, including aspects from approximation theory, image processing, numerics, partial differential equations, and statistics, with a strong focus on numerical methods in image processing. Providing a systematic and general framework for image registration, the book not only presents state-of-the-art concepts but also summarises and classifies the numerous techniques to be found in the literature.

This book is a comprehensive review of a myriad of cases, a practical board review, a resource for maintenance of certification, and an interesting read with relevant images. The cases are real-life examples seen by radiologists around the United States. It is beneficial to the practicing radiologists, residents and fellows. A comprehensive ultrasound review book in a case-based format. Contains 125 cases to give a practical review. Each case includes a thorough discussion of ultrasound findings, many including cross-sectional correlation. Differential diagnoses are presented along with diagnostic pearls and management options.

This book presents a thorough review of coronary angioscopy, ranging from instructions on its use to the latest advances. Starting with the structure and fundamental principles of angioscopy, it shows readers how to apply the image to comprehensive care of coronary-artery patients. Plentiful color photos and illustrations will enable readers to investigate and classify plaques and thrombi and to evaluate coronary stent- and drug-based therapies. The authors are leading researchers on angioscopy. This book offers the perfect guide not only for new clinicians but also for cardiologists who have already adopted this technique for medical examination and treatment. Angioscopy is a unique medical technique for visualizing the interior of blood vessels and helps physicians not only to diagnose the pathology but also to measure the effectiveness of Percutaneous coronary intervention (PCI) or antiarteriosclerotic drugs. Furthermore, the recently developed molecular angioscopy approach allows us to observe Low-density lipoprotein (LDL) oxide, collagen, and macrophages, and is rapidly growing in importance.

Oncological imaging has thoroughly changed in the past decade, especially due to the introduction of PET and 18FDG. In "Positron Emission Tomography," expert referring specialists and professional imagers seek to help bridge some of the knowledge gaps in several oncological domains. The book s goal is to aid in the improvement of communicative competences: to communicate scan findings so that the referring specialist receives proper advice from the imager, and that, alternatively, the referring one provides the imager with appropriate clinical details to allow for a proper interpretation, and that the referring specialist is aware of the possibilities and limitations of the requested technology. While it focuses on FDG PET, other radiopharmaceuticals are covered as well, where appropriate. Written for the highly respected "Methods in Molecular Biology " series, this volume provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

Authoritative and convenient, "Positron Emission Tomography" serves as an excellent reference for oncologists, surgeons, radiotherapists, radiologists, nuclear medicine physicians, and pathologists desiring a stronger synergy within their vital efforts."

The book discusses the impact of machine learning and computational intelligent algorithms on medical image data processing, and introduces the latest trends in machine learning technologies and computational intelligence for intelligent medical image analysis. The topics covered include automated region of interest detection of magnetic resonance images based on center of gravity; brain tumor detection through low-level features detection; automatic MRI image segmentation for brain tumor detection using the multi-level sigmoid activation function; and computer-aided detection of mammographic lesions using convolutional neural networks.

An in-depth introduction to radiotherapy physics emphasizing the clinical aspects of the field. This second edition gradually and sequentially develops each of its topics in clear and concise language. It includes important mathematical analyses, yet is written so that these sections can be skipped, if desired, without compromising understanding. The book consists of seven parts covering basic physics (Parts I-II), equipment for radiotherapy (Part III), radiation dosimetry (Parts IV-V), radiation treatment planning (Part VI), and radiation safety and shielding (Part VII). An invaluable text for radiation oncologists, radiation therapists, and clinical physicists.

This book represents the first comprehensive textbook devoted to the standard of care, current guidelines and innovations in the field of mesenteric vascular disease. The book reviews imaging modalities, diagnostic work up, physiologic tests, traditional open surgical techniques and novel endovascular approaches. Technical aspects of both open surgical and endovascular techniques are provided by experts in the field, with illustrations and photographs of key steps for each type of procedure. Results of epidemiologic studies and national databases are summarized, as well as large institutional experiences. An evidence-based approach is used for recommendations regarding best therapies. Diagnostic approaches including imaging and novel physiologic tests, including gastric tonometry and oxygen light spectroscopy are covered. Mesenteric Vascular Disease: Current Therapy will serve as a very useful resource for clinicians, surgeons, interventionalists, gastroenterologists and researchers dealing with and interested in mesenteric vascular diseases.

Drug development today needs to balance agility, speed, and risk in defining probability of success for molecules, mechanisms, and therapeutic concepts. New techniques such as fMRI promise to be part of a sequence that could transform drug development. Although numerous review articles exist that discuss the use of imaging in drug development, no one source is available that combines the various techniques and includes a discussion of disease mapping.

Imaging in CNS Drug Discovery and Development, Implications for Disease and Therapy will serve to distill the most salient developments in the use of imaging in drug development and disease mapping. It will launch evolving concepts that integrate new imaging technologies and paradigms with molecular medicine and molecular profiling ("monics") as well as consider the ethical issues that arise as a result of disease or state diagnosis and the use of imaging in the public eye.

Neuroimaging in Neurogenic Communication Disorders provides a comprehensive review of cases utilizing neuroimaging in neurogenic communication disorders. Basic knowledge of neuroanatomy and medical conditions related to these speech and language disorders are discussed. Each case study includes information on neuroanatomy, case presentation, neuroimaging, differential diagnosis, and final diagnosis. This book is written for medical students, practitioners and researchers in neuroscience and speech language pathology. Neurogenic communication disorders are caused by damage to the central or peripheral nervous system. This damage can be caused by Parkinson's disease, stroke, dementia, traumatic brain injury, brain tumors, and other neurologic disorders and causes issues such as aphasia, dysarthria and apraxia.

Over the past decade, we have made great advances in the field of multiple sclerosis (MS) research, and this book focuses on those advances in MS pathogenesis and treatment. While some of these advances have been through new approaches and ideas that have emerged in the last decade such as the newly identified protective role that amyloid proteins may play in MS or the use of helminths to treat autoimmune diseases, others have evolved from previous theories and ideas that have only now gained momentum and a deeper understanding such as the role of HLA or gender in MS susceptibility. This book covers these emerging and evolving topics and highlights the substantial advancements made in elucidation of the factors regulating susceptibility or disease progression, identification of new ways to monitor or predict MS pathology, and development of new strategies for treating MS.

This third edition is a simulator for your clinical mind and exclusively designed to give you the final edge over intense competition. The book is a compilation of all the possible clinical scenarios which have been asked in various examinations and may be repeated in the future. This book provides a comprehensive and easy-to-grasp insight into the world of image-based questions. The new pattern of examination has loads of image-based MCQs, creating an unpredictable atmosphere and doubts in even the best of brains. This compilation is dedicated to all those young inquisitive minds, who have endured all the long study hours, feeling of despair and finally, relief when the results are out.

Senior scientists from neighboring and other NATO countries joined their efforts to help this region to get to know their problems, discussed their solutions and how they can be helped out. Distinguished experts described how they had succeeded in developing the solutions to such problem in their countries.

Practical Manual of Echocardiography in the Urgent Setting In the acute care setting, medicine happens at full speed and with little margin for error. As echocardiography plays an ever more important role in the diagnosis of patients who present with symptoms that suggest a cardiovascular emergency, clinicians must learn to collect, process and act on echocardiographic information as quickly and effectively as possible. Practical Manual of Echocardiography in the Urgent Setting covers the essentials of echocardiography in the acute setting, from ultrasound basics to descriptions of all pertinent echocardiographic views to clear, stepwise advice on basic calculations and normal/abnormal ranges. This compact new reference: Provides step-by-step guidance to acquiring the correct views and making the necessary calculations to accurately diagnose cardiac conditions commonly encountered in urgent settings. Presents information organized by complaint/initial presentation so that readers can work from this first knowledge of the patient through the steps required to pinpoint a diagnosis. Covers echo basics, from sound wave characteristics/properties to common device settings to basic ultrasound formulas Includes diagnostic algorithms fitted to address the differential diagnosis in the most commonlyencountered clinical scenarios. Designed and written by frontline clinicians with extensive experience treating patients, Practical Manual of Echocardiography in the Urgent Setting is the perfect pocket-sized guide for residents in cardiology, emergency medicine, and hospital medicine; trainees in echocardiography; medical students on cardiology or emergency medicine rotations; technicians, nurses, attending physicians--anyone who practices in the urgent setting and who needs reliable guidance on echocardiographic views, data and normal/abnormal ranges to aid rapid diagnosis and decision-making at the point of care. RELATED TITLES: Kacharava, et al: Pocket Guide to Echocardiography; ISBN: 978-0-470-67444-4 Sun, et al: Practical Handbook of Echocardiography: 101 Case Studies; ISBN: 978-1-4051-9556-0

Positron emission tomography (PET) has been at the forefront of fu- tional and molecular imaging for a number of years. The future of di- nostic imaging depends upon the ability to change from imaging anatomy to examining the processes at work in the body. The fact that there are now monographs examining particular aspects of PET, such as this book on the examination of children, speaks to the newly won maturity of PET. The authors are to be congratulated for the timely appearance of this volume. In recent years, PET has transformed the contributions of nuclear medicine to the diagnosis, staging, and follow-up of patients with cancer. Children with cancer deserve the very best and most comp- sionate care that society can provide. Ultimately the greatest comp- sion we can offer as physicians is to provide the best possible care. Those charged with creating public policy in the context of diagnostic medicine must make common cause with physicians and other sci- tists to ensure that that best possible care is realized at the bedside. All of the evidence suggests that PET is central to such optimal cancer care. In addition to the distinguished cast of physicians and researchers who contributed to this book, I welcome the contributions from te- nologists who are a key part of the interaction between the diagnostic process and the sick or potentially sick child. Good care is contingent upon putting parents and child at ease, and the technologist has a lead role in this.

This volume builds on the success of the first edition of Imaging Pelvic Floor Disorders and is aimed at those practitioners with an interest in the imaging, diagnosis and treatment of pelvic floor dysfunction. Concise textual information from acknowledged experts is complemented by high-quality diagrams and images to provide a thorough update of this rapidly evolving field. Introductory chapters fully elucidate the anatomical basis underlying disorders of the pelvic floor. State of the art imaging techniques and their application in pelvic floor dysfunction are then discussed in detail. Additions since the first edition include consideration of the effect of aging and new chapters on perineal ultrasound, functional MRI and MRI of the levator muscles. The closing sections of the book describe the modern clinical management of pelvic floor dysfunction, including prolapse, urinary and faecal incontinence and constipation, with specific emphasis on the integration of diagnostic and treatment algorithms. Written for: Practitioners and clinicians in the fields radiology, urology, proctology/colorectal surgery, gynecology, gastroenterology

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis.
Key Features
* Authored by the key leaders in the biological electron microscopy field
* Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines
* Introduces students on how to read and interpret electron micrographs

This book addresses patient-specific modeling. It integrates computational modeling, experimental procedures, imagine clinical segmentation and mesh generation with the finite element method (FEM) to solve problems in computational biomedicine and bioengineering. Specific areas of interest include cardiovascular problems, ocular and muscular systems and soft tissue modeling. Patient-specific modeling has been the subject of serious research over the last seven years and interest in the area is continually growing and this area is expected to further develop in the near future.

This is the third volume in the series, in which the topic of the effects of radio frequencies on human tissue, now increasingly a concern with the prevalence of cell phones, is explored by Prof. Lin and other researchers. The impact of electromagnetics on imaging and cardiology, both very keen areas of research at present, is also explored.

Reflecting the expanding field's need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both 'novice' and established scientists toward furthering their research with these invaluable techniques.

This book presents important recent developments in mathematical and computational methods used in impedance imaging and the theory of composite materials. By augmenting the theory with interesting practical examples and numerical illustrations, the exposition brings simplicity to the advanced material. An introductory chapter covers the necessary basics. An extensive bibliography and open problems at the end of each chapter enhance the text.