A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular Biology (TM) series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.

This book presents commonly applied characterization techniques in material science, their brief history and origins, mechanism of operation, advantages and disadvantages, their biosensing applications, and troubleshooting for each technique, while addressing the challenges researchers face when working with these techniques. The book dedicates its focus to identifying physicochemical and electrochemical nature of materials including analyses of morphology, mass spectrometry, and topography, as well as the characterization of elemental, structural, thermal, wettability, electrochemical, and chromatography properties. Additionally, the main features and benefits of using coupled characterization techniques are discussed in this book.

This thesis reports advances in terahertz time-domain spectroscopy, relating to the development of new techniques and components that enhance the experimentalist's control over the terahertz polarisation state produced by photoconductive emitters. It describes how utilising the dynamic magnetoelectric response at THz frequencies, in the form of electromagnons, can probe material properties at a transition between two magnetically ordered phases. Additionally, preliminary investigations into the properties of materials exposed to extreme terahertz optical electric fields are reported. The work presented in this thesis may have immediate impacts on the study of anisotropic media at THz frequencies, with photoconductive emitters and detectors being the most commonly used components for commercially available terahertz spectroscopy and imaging systems, and by providing a new way to study the nature of magnetic phase transitions in multiferroics. In the longer term the increased understanding of multiferroics yielded by ultrafast spectroscopic methods, including terahertz time-domain spectroscopy, may help develop new magnetoelectric and multiferroic materials for applications such as spintronics.

Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 80 years the Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.

This book focuses on the metallic Nano- and Micro-materials (NMMs) fabricated by physical techniques such as atomic diffusion. A new technology for fabricating NMMs by atomic diffusion is presented. Two kinds of atomic diffusion are treated; one is a phenomenon caused by electron flow in high density and called electromigration and the other is stress migration which depends on a gradient of hydrostatic stress in a material.

In three parts, the book describes the theory of atomic diffusion, the evaluation of physical properties and the treatment and applications of metallic NNMS. The new methods such as atomic diffusion are expected are expected to be crucial for the fabrication of NNMs in the future and to partially replace methods based on chemical reactions.

Principles and Practice of Modern Chromatographic Methods, Second Edition takes a comprehensive, unified approach in its presentation of chromatographic techniques. Like the first edition, the book provides a scientifically rigid, but easy-to-follow presentation of chromatography concepts that begins with the purpose and intent of chromatographic theory - the "what and why" that are left out of other books attempting to cover these principles. This fully revised second edition brings the content up-to-date, covering recent developments in several new sections and an additional chapter on composite methods. New topics include sample profiling, sample preparation, sustainable green chemistry, 2D chromatography, miniaturization/nano-LC, HILIC, and more.

Counterterrorist Detection Techniques of Explosives, Second Edition covers the most current techniques available for explosive detection. This completely revised volume describes the most updated research findings that will be used in the next generation of explosives detection technologies. New editors Drs. Avi Cagan and Jimmie Oxley have assembled in one volume a series of detection technologies written by an expert group of scientists. The book helps researchers to compare the advantages and disadvantages of all available methods in detecting explosives and, in effect, allows them to choose the correct instrumental screening technology according to the nature of the sample.

This book focuses on the modern development of techniques for analysis of the hierarchical structure of polymers from both the experimental and theoretical points of view. Starting with molecular and crystal symmetry, the author explains fundamental and professional methods, such as wide- and small-angle X-ray scattering, neutron diffraction, electron diffraction, FTIR and Raman spectroscopy, NMR, and synchrotron radiation. In addition, the author explains another indispensable method, computer simulation, which includes energy calculation, lattice dynamics, molecular dynamics, and quantum chemistry. These various methods are described in a systematic way so that the reader can utilize them for the purpose of 3D structure analysis of polymers. Not only such analytical knowledge but also the preparation techniques of samples necessary for these measurements and the methods of analyzing the experimental data collected in this way are given in a concrete manner. Examples are offered to help master the principles of how to clarify the static structures and dynamic structural changes in the phase transitions of various kinds of crystalline polymers that are revealed by these novel methods. The examples are quite useful for readers who want to apply these techniques in finding practical solutions to concrete problems that are encountered in their own research. The principal audience for this book is made up of young professional researchers including those working in industry, but it can also be used as an excellent reference for graduate-level students. This book is the first volume of a two-volume set with Structural Science of Crystalline Polymers: A Microscopically Viewed Structure-Property Relationship being the second volume by the same author.

The book highlights recent developments in the field of spectroscopy by providing the readers with an updated and high-level of overview. The focus of this book is on the introduction to concepts of modern spectroscopic techniques, recent technological innovations in this field, and current examples of applications to molecules and materials relevant for academia and industry. The book will be beneficial to researchers from various branches of science and technology, and is intended to point them to modern techniques, which might be useful for their specific problems. Spectroscopic techniques, that are discussed include, UV-Visible absorption spectroscopy, XPS, Raman spectroscopy, SERS, TERS, CARS, IR absorption spectroscopy, SFG, LIBS, Quantum cascade laser (QCL) spectroscopy, fluorescence spectroscopy, ellipsometry, cavity-enhanced absorption spectroscopy, such as cavity ring-down spectroscopy (CRDS) and evanescent wave-CRDS both in gas and condensed phases, time-resolved spectroscopy etc. Applications introduced in the different chapters demonstrates the usefulness of the spectroscopic techniques for the characterization of fundamental properties of molecules, e.g. in connection with environmental impact, bio-activity, or usefulness for pharmaceutical drugs, and materials important e.g. for nano-science, nuclear chemistry, or bio-applications. The book presents how spectroscopic techniques can help to better understand substances, which have also great impact on questions of social and economic relevance (environment, alternative energy, etc.).

This thesis presents a systematic discussion of experimental approaches to investigating the nonlinear interaction of ultrashort visible strong fields with dielectrics directly in the time domain. The key finding is the distinctly different peak-intensity dependence of the light-matter energy transfer dynamics on the one hand, and the observed transient optical and electronic modifications on the other. As the induced electron dynamics evolve on sub-femtosecond timescales, real-time spectroscopy requires attosecond temporal resolution. This allows a range of parameters to be identified where the optical properties of the samples exposed to ultrashort light fields suffer dramatic changes allowing signal metrology while real absorption leading to dissipation is essentially absent. These findings indicate the feasibility of efficient optical switching at frequencies several orders of magnitude faster than current state-of-the-art electronics and thus have far-reaching technological consequences.

This book presents the high-precision analysis of ground states and low-energy excitations in fractional quantum Hall states formed by Dirac electrons, which have attracted a great deal of attention. In particular the author focuses on the physics of fractional quantum Hall states in graphene on a hexagonal boron nitride substrate, which was recently implemented in experiments. The numerical approach employed in the book, which uses an exact numerical diagonalization of an effective model Hamiltonian on a Haldane's sphere based on pseudopotential representation of electron interaction, provides a better understanding of the recent experiments. The book reviews various aspects of quantum Hall effect: a brief history, recent experiments with graphene, and fundamental theories on integer and fractional Hall effects. It allows readers to quickly grasp the physics of quantum Hall states of Dirac fermions, and to catch up on latest research on the quantum Hall effect in graphene.

In this thesis, the author outlines the development of new monolithic columns and isotope dimethyl labeling strategies and their applications in high-performance proteome analyses. Though different types of monolithic columns have been widely developed for chromatography and electrophoresis separation, their application in proteomics for complex peptide mixtures separation is still a challenge. The author discusses the preparation of new monolithic columns and optimization of chromatography separation capability to improve coverage and accuracy of proteome analysis. Further, the author describes a novel online multidimensional chromatography system combined with automated online isotope labeling, which significantly improves the throughput, sensitivity and accuracy of quantitative proteomics. In addition to the development of new technologies, the author investigates the proteome and phosphoproteome expression changes of clinical hepatocellular carcinoma tissues and the hippocampi of mice with Alzheimer s disease. The work in this thesis has led to several publications in high-profile journals in the fields of analytical chemistry and proteome research."

This book provides an understandable review of SU(3) representations, SU(3) Wigner-Racah algebra and the SU(3) SO(3) integrity basis operators, which are often considered to be difficult and are avoided by most nuclear physicists. Explaining group algebras that apply to specific physical systems and discussing their physical applications, the book is a useful resource for researchers in nuclear physics. At the same time it helps experimentalists to interpret data on rotational nuclei by using SU(3) symmetry that appears in a variety of nuclear models, such as the shell model, pseudo-SU(3) model, proxy-SU(3) model, symplectic Sp(6, R) model, various interacting boson models, various interacting boson-fermion models, and cluster models. In addition to presenting the results from all these models, the book also describes a variety of statistical results that follow from the SU(3) symmetry.

Annual Reports on NMR Spectroscopy, Volume 104 has established itself as a premier resource for both specialists and non-specialists who are looking to become familiar with new techniques and applications pertaining to NMR spectroscopy.

This book provides insight into the underlying basic theories and concepts in X-ray, light, and neutron scattering. The three scattering principles are systematically presented, together with a unified description based on elastic scattering of electromagnetic waves and the Schroedinger wave from matter. These explanations are presented with an introduction of their common Born approximation using a consistent set of symbols and terminology and with step-by-step derivations of equations. This book emphasizes the combined applications of these three scattering methods, wherever and whenever possible, as a very powerful methodology for characterization of internal structures of soft matters in the length scale ranging from subnanometers to a few 10 micron meters. These applications include explorations for evolution of hierarchically self-organized internal structures of a variety of soft matters, including cells, under diverse environmental conditions. This book will not only be an excellent resource for graduate students and academic researchers who analyze structures of soft matters and polymers, but it will also be useful for researchers in industries.

This book is intended for both the undergraduate and postgraduate levels. The subject material deals with symmetry, starting from the basic and rudimental foundations of the concept and the depth of its applications. The presentation of the concepts and the related illustrations in the book are on semi-pedagogical lines and thus offer easy understanding to the reader. The book is designed with twelve well thought chapters, with each preceding chapter opening the gate for the development of the next thereby having a high degree of sequence.The first seven chapters are devoted to the indepth understanding of 'symmetry and group theory' of molecules and the last five chapters are developed on the aspects of how these formalisms are utilized for the 'structure, spectra and bonding' of molecules. The book also covers the essential basics of the group theory that are required for all sections of chemistry, and emphasizes the necessity of this theory to understand the theoretical and applied aspects of molecular spectroscopy. This text is the result of a long felt need for developing certain novel techniques for the teaching of this course. A 'Window-vision' has been provided in the book while presenting most of the chapters and 'Study Questions' are placed at the end of each chapter to help ensure understanding of the concepts. 'No more nightmares of group theory and spectroscopy' - is the ultimate purpose of this book.

As a spectroscopic method, Nuclear Magnetic Resonance (NMR) has seen spectacular growth over the past two decades, both as a technique and in its applications. Today the applications of NMR span a wide range of scientific disciplines, from physics to biology to medicine. Each volume of Nuclear Magnetic Resonance comprises a combination of annual and biennial reports which together provide comprehensive of the literature on this topic. This Specialist Periodical Report reflects the growing volume of published work involving NMR techniques and applications, in particular NMR of natural macromolecules which is covered in two reports: "NMR of Proteins and Acids" and "NMR of Carbohydrates, Lipids and Membranes." For those wanting to become rapidly acquainted with specific areas of NMR, this title provides unrivalled scope of coverage. Seasoned practitioners of NMR will find this an in valuable source of current methods and applications. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject areas, the series creates a unique service for the active research chemist, with regular, in-depth accounts of progress in particular fields of chemistry. Subject coverage within different volumes of a given title is similar and publication is on an annual or biennial basis.

This volume collects a a number of contributions on spontaneous symmetry breaking. Current studies in this general field are going ahead at a full speed. The book present review chapters which give an overview on the major break throughs of recent years. It covers a number of different physical settings which are introduced when a nonlinearity is added to the underlying symmetric problems and its strength exceeds a certain critical value. The corresponding loss of symmetry, called spontaneous symmetry breaking, alias self-trapping into asymmetric states is extensively discussed in this book.
The book presents both active theoretical studies of spontaneous symmetry breaking effects as well as experimental findings, chiefly for Bose-Einstein-Condensates with the self-repulsive nonlinearity, and also for photorefractive media in optics.

This book covers the fundamental aspects of fiber lasers and fiber amplifiers, and includes a wide range of material from laser physics fundamentals to state-of-the-art topics in this rapidly growing field of quantum electronics. This expanded and updated new edition includes substantial new material on nonlinear frequency conversion and Raman fiber lasers and amplifiers, as well as an expanded list of references inclusive of the recent literature in the field. Emphasis is placed on the nonlinear processes taking place in fiber lasers and amplifiers, their similarities, differences to, and their advantages over other solid-state lasers. The reader will learn the basic principles of solid-state physics and optical spectroscopy of laser active centers in fibers, the main operational laser regimes, and will receive practical recommendations and suggestions on fiber laser research, laser applications, and laser product development. The book will be useful for students, researchers, and professional physicists and engineers who work with lasers in the optical and telecommunications field, as well as those in the chemical and biological industries.

Device Architecture and Materials for Organic Light-Emitting Devices focuses on the design of new device and material concepts for organic light-emitting devices, thereby targeting high current densities and an improved control of the triplet concentration. A new light-emitting device architecture, the OLED with field-effect electron transport, is demonstrated. This device is a hybrid between a diode and a field-effect transistor. Compared to conventional OLEDs, the metallic cathode is displaced by one to several micrometers from the light-emitting zone, reducing optical absorption losses. The electrons injected by the cathode accumulate at an organic heterojunction and are transported to the light-emission zone by field-effect. High mobilities for charge carriers are achieved in this way, enabling a high current density and a reduced number of charge carriers in the device. Pulsed excitation experiments show that pulses down to 1 us can be applied to this structure without affecting the light intensity, suggesting that pulsed excitation might be useful to reduce the accumulation of triplets in the device. The combination of all these properties makes the OLED with field-effect electron transport particularly interesting for waveguide devices and future electrically pumped lasers. In addition, triplet-emitter doped organic materials, as well as the use of triplet scavengers in conjugated polymers are investigated.

Mass spectrometry (MS) along with its hyphenated techniques is capable of high throughput, sensitivity, accuracy and selectivity for the analysis of structure and composition of almost any product. Like in electrophoresis, MS separates mo- cules based on the mass-to-charge ratio. In case of gel electrophoresis (SDS- PAGE), a well-known and efficient bioanalytical technique, proteins bear negative charges but have the same charge density, so proteins are separated according to their size. Similarly, in case of MS analysis, proteins carry the same charge, and are separated by their molecular weight. Unlike SDS-PAGE, however, modern ultra high resolution MS discerns very small mass differences and can resolve and completely identify in a single experiment species of the same nominal mass in complex biological mixtures. Consequently, MS can be used for the structural characterization, identification and sensitive detection of mixtures of biomolecules or for assessing the quality of isolated proteins (purity, integrity, or post-translational modifications, for example), carbohydrates, nucleic acids, drugs, metabolites, pollutants etc. In the post-genome era, MS is continuously developing as one of the most re- able analytical method for elucidating the structure of molecules originating from various biological matrices. The potential of MS for high-sensitive structural a- lyses became unsurpassable after the introduction of electrospray (ESI) and matrix assisted laser/desorption ionization (MALDI) methods, on one hand, and the pos- bility to deduce in detail unknown biopolymer structures by highly accurate mo- cular mass measurement followed by sequencing using dissociation techniques based on multiple stage MS, on the other.

This book provides a multidisciplinary overview to the application of high order derivative spectrophotometry and Electron Spin Resonance (ESR) spectroscopy in biology and ecology. The characteristics of the principle methods as well as the generation of reliable spectra are discussed in general terms allowing the reader to gain an idea of these methods' potentials. Furthermore the authors give an extended overview to the spectroscopic and spectro-photometric analysis of specific biological materials. This volume is a well condensed description of an analytical method and a clear review to its application in biology and related fields and an essential tool for researchers who are new in the field of spectroscopic methods and their applications in the life sciences.

Spectroscopic Properties of Inorganic and Organometallic Compounds provides a unique source of information on an important area of chemistry. Divided into sections mainly according to the particular spectroscopic technique used, coverage in each volume includes: NMR (with reference to stereochemistry, dynamic systems, paramagnetic complexes, solid state NMR and Groups 13-18); nuclear quadrupole resonance spectroscopy; vibrational spectroscopy of main group and transition element compounds and coordinated ligands; and electron diffraction. Reflecting the growing volume of published work in this field, researchers will find this Specialist Periodical Report an invaluable source of information on current methods and applications. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading experts in their specialist fields, this series is designed to help the chemistry community keep current with the latest developments in their field. Each volume in the series is published either annually or biennially and is a superb reference point for researchers. www.rsc.org/spr

Fifth volume of a 40 volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about X-ray and Neutron Techniques for Nanomaterials Characterization. Modern applications and state-of-the-art techniques are covered and make this volume an essential reading for research scientists in academia and industry.

Fabrication technologies for nanostructured devices have been developed recently, and the electrical and optical properties of such nanostructures are a subject of advanced research.This book describes the different approaches to spectroscopic microscopy, i.e., Electron Beam Probe Spectroscopy, Spectroscopic Photoelectron Microscopy, and Scanning Probe Spectroscopy. It will be useful as a compact source of reference for the experienced reseracher, taking into account at the same time the needs of postgraduate students and nonspecialist researchers by using a tutorial approach throughout.