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Books > Science & Mathematics > Chemistry > Analytical chemistry

Synthesis, Characterization and Reactivity of Ylidyne and  -Ylido Complexes Supported by Scorpionato Ligands (Hardcover, 1st... Synthesis, Characterization and Reactivity of Ylidyne and -Ylido Complexes Supported by Scorpionato Ligands (Hardcover, 1st ed. 2019)
Priyabrata Ghana
R2,925 Discovery Miles 29 250 Ships in 10 - 15 working days

This book explores the development of the first open-shell heavier tetrylidyne complexes featuring a tetrel-centered unpaired electron, and unprecedented metallatetrylidynes containing a multiply-bonded, linear-coordinated single heavier tetrel atom embedded between two metal centers. The chemistry of compounds featuring triple bonds of the heavier Group-14 elements Si-Pb with transition metals is a very challenging research area, which combines modern molecular main-group element with transition-metal chemistry, and is of fundamental importance for the understanding of chemical bonding. During the last 15 years, the research in this area has witnessed considerable progress in isolating a series of closed-shell tetrylidyne complexes. However, despite numerous attempts, open-shell tetrylidyne complexes and heavier group 14 element congeners of metallacarbynes and carbide complexes remained inaccessible. In this book, readers will find more about the synthesis, full characterization and reactivity studies of these novel complexes that uncovered a plethora of exceptional products, including a novel m3-silicido complex, the first dimetallasilacumulene with a linear, two-coordinated single silicon atom and the first compounds of planar tetracoordinated silicon (ptSi) (Anti-van't Hoff-Le Bell Silicon). Readers will also learn about the isolation and full characterization of the first room-temperature stable disilavinylidene, a silicon analogue of the very reactive vinylidenes (R2C=C:), and the first intermetallic plumbylidyne ligand transfer reactions.

Nuclear Magnetic Resonance - Volume 12 (Hardcover, Edition.): G.A. Webb Nuclear Magnetic Resonance - Volume 12 (Hardcover, Edition.)
G.A. Webb
R10,909 Discovery Miles 109 090 Ships in 10 - 15 working days

As a spectroscopic method, Nuclear Magnetic Resonance (NMR) has seen spectacular growth over the past two decades, both as a technique and in its applications. Today the applications of NMR span a wide range of scientific disciplines, from physics to biology to medicine. Each volume of Nuclear Magnetic Resonance comprises a combination of annual and biennial reports which together provide comprehensive of the literature on this topic. This Specialist Periodical Report reflects the growing volume of published work involving NMR techniques and applications, in particular NMR of natural macromolecules which is covered in two reports: "NMR of Proteins and Acids" and "NMR of Carbohydrates, Lipids and Membranes." For those wanting to become rapidly acquainted with specific areas of NMR, this title provides unrivalled scope of coverage. Seasoned practitioners of NMR will find this an in valuable source of current methods and applications. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject areas, the series creates a unique service for the active research chemist, with regular, in-depth accounts of progress in particular fields of chemistry. Subject coverage within different volumes of a given title is similar and publication is on an annual or biennial basis.

Whole Cell Sensing System II - Applications (Hardcover, Edition.): Shimshon Belkin, Man Bock Gu Whole Cell Sensing System II - Applications (Hardcover, Edition.)
Shimshon Belkin, Man Bock Gu
R5,597 Discovery Miles 55 970 Ships in 10 - 15 working days

Applications: - Applications of Microbial Cell Sensors, by Mifumi Shimomura-Shimizu and Isao Karube - Whole-Cell Bioreporters for the Detection of Bioavailable Metals, by Anu Hynninen and Marko Virta - Bacteriophage-Based Pathogen Detection, by Steven Ripp - Cell-Based Genotoxicity Testing, by Georg Reifferscheid and Sebastian Buchinger - Cytotoxicity and Genotoxicity Reporter Systems Based on the Use of Mammalian Cells, by Christa Baumstark-Khan, Christine E. Hellweg, and Gunther Reitz - Live Cell Optical Sensing for High Throughput Applications, by Ye Fang - Cyanobacterial Bioreporters as Sensors of Nutrient Availability, by George S. Bullerjahn, Ramakrishna Boyanapalli, Mark J. Rozmarynowycz, and R. Michael L. McKay - Application of Microbial Bioreporters in Environmental Microbiology and Bioremediation, by E. E. Diplock, H. A. Alhadrami, and G. I. Paton

NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces (Hardcover): Anirban Bhunia, Hanudatta S. Atreya,... NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces (Hardcover)
Anirban Bhunia, Hanudatta S. Atreya, Neeraj Sinha
R6,705 Discovery Miles 67 050 Ships in 10 - 15 working days

NMR spectroscopy has found a wide range of applications in life sciences over recent decades. Providing a comprehensive amalgamation of the scattered knowledge of how to apply high-resolution NMR techniques to biomolecular systems, this book will break down the conventional stereotypes in the use of NMR for structural studies. The major focus is on novel approaches in NMR which deal with the functional interface of either protein-protein interactions or protein-lipid interactions. Bridging the gaps between structural and functional studies, the Editors believe a thorough compilation of these studies will open an entirely new dimension of understanding of crucial functional motifs. This in turn will be helpful for future applications into drug design or better understanding of systems. The book will appeal to NMR practitioners in industry and academia who are looking for a comprehensive understanding of the possibilities of applying high-resolution NMR spectroscopic techniques in probing biomolecular interactions.

Nanoscale Imaging and Characterisation of Amyloid- (Hardcover, 1st ed. 2016): Claire Louisa Tinker-Mill Nanoscale Imaging and Characterisation of Amyloid- (Hardcover, 1st ed. 2016)
Claire Louisa Tinker-Mill
R3,467 Discovery Miles 34 670 Ships in 12 - 19 working days

This thesis presents a method for reliably and robustly producing samples of amyloid- (A ) by capturing them at various stages of aggregation, as well as the results of subsequent imaging with various atomic force microscopy (AFM) methods, all of which add value to the data gathered by collecting information on the peptide's nanomechanical, elastic, thermal or spectroscopical properties. Amyloid- (A ) undergoes a hierarchy of aggregation following a structural transition, making it an ideal subject of study using scanning probe microscopy (SPM), dynamic light scattering (DLS) and other physical techniques. By imaging samples of A with Ultrasonic Force Microscopy, a detailed substructure to the morphology is revealed, which correlates well with the most advanced cryo-EM work. Early stage work in the area of thermal and spectroscopical AFM is also presented, and indicates the promise these techniques may hold for imaging sensitive and complex biological materials. This thesis demonstrates that physical techniques can be highly complementary when studying the aggregation of amyloid peptides, and allow the detection of subtle differences in their aggregation processes.

XUV Pump-Probe Experiments on Diatomic Molecules - Tracing the Dynamics of Electron Rearrangement and Interatomic Coulombic... XUV Pump-Probe Experiments on Diatomic Molecules - Tracing the Dynamics of Electron Rearrangement and Interatomic Coulombic Decay (Hardcover, 2015 ed.)
Kirsten Schnorr
R3,786 R3,504 Discovery Miles 35 040 Save R282 (7%) Ships in 12 - 19 working days

This book explores the relaxation dynamics of inner-valence-ionized diatomic molecules on the basis of extreme-ultraviolet pump-probe experiments performed at the free-electron laser (FEL) in Hamburg. Firstly, the electron rearrangement dynamics in dissociating multiply charged iodine molecules is studied in an experiment that made it possible to access charge transfer in a thus far unexplored quasimolecular regime relevant for plasma and chemistry applications of the FEL. Secondly the lifetime of an efficient non-radiative relaxation process that occurs in weakly bound systems is measured directly for the first time in a neon dimer (Ne2). Interatomic Coulombic decay (ICD) has been identified as the dominant decay mechanism in inner-valence-ionized or excited van-der-Waals and hydrogen bonded systems, the latter being ubiquitous in all biomolecules. The role of ICD in DNA damage thus demands further investigation, e.g. with regard to applications like radiation therapy.

Metal-to-Nonmetal Transitions (Hardcover, 2010 Ed.): Ronald Redmer, Friedrich Hensel, Bastian Holst Metal-to-Nonmetal Transitions (Hardcover, 2010 Ed.)
Ronald Redmer, Friedrich Hensel, Bastian Holst
R2,885 Discovery Miles 28 850 Ships in 10 - 15 working days

This book is devoted to nonmetal-to-metal transitions. The original ideas of Mott for such a transition in solids have been adapted to describe a broad variety of phenomena in condensed matter physics (solids, liquids, and fluids), in plasma and cluster physics, as well as in nuclear physics (nuclear matter and quark-gluon systems). The book gives a comprehensive overview of theoretical methods and experimental results of the current research on the Mott effect for this wide spectrum of topics. The fundamental problem is the transition from localized to delocalized states which describes the nonmetal-to-metal transition in these diverse systems. Based on the ideas of Mott, Hubbard, Anderson as well as Landau and Zeldovich, internationally respected scientists present the scientific challenges and highlight the enormous progress which has been achieved over the last years. The level of description is aimed to specialists in these fields as well as to young scientists who will get an overview for their own work. A common feature of all contribution is the extensive discussion of bound states," i.e. their formation and dissolution due to medium effects. This applies to atoms and molecules in plasmas, fluids, and small clusters, excitons in semiconductors, or nucleons, deuterons, and alpha-particles in nuclear matter. In this way, the transition from delocalized to localized states and vice versa can be described on a common level."

Mass Spectrometry - Volume 1 (Hardcover, Edition.): D.H. Williams Mass Spectrometry - Volume 1 (Hardcover, Edition.)
D.H. Williams
R10,871 Discovery Miles 108 710 Ships in 10 - 15 working days

Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 80 years the Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.

Advances in Coal Spectroscopy (Hardcover, 1992 ed.): Henk Meuzelaar Advances in Coal Spectroscopy (Hardcover, 1992 ed.)
Henk Meuzelaar
R4,613 Discovery Miles 46 130 Ships in 10 - 15 working days

The past decade has witnessed major advances in our understanding of the chemical composition, structure, and reactivity of the complex organic-rich fossil matter known as "coal. " Nevertheless, important scientific questions concerning molecular weight distributions, degree of crosslinking, typical duster sizes, type of interconnecting bridges, the possible role of a "mobile phase," and the nature of organic sulfur forms remain topics of heated debate. Moreover, there appears to be a notable lack of consensus regarding the overall direction and goals of structural elucidation work. Is it worthwhile to study whole coal samples, or should we separate out the various, more or less well-defined, maceral and mineral constituents before attempting to describe the structural and compositional features of coal at the molecular Ievel? Second, should there be more emphasis on key structural features and average statistical parameters, or is it necessary to identify individual chemical structures in considerable detail? From the developments of the past decade it is clear that advanced spectroscopic techniques are playing an increasingly important role in resolving difficult questions with regard to the chemical structure and composition of coal. Moreover, it has become equally clear that no single spectroscopic approach can provide all the answers but multiple techniques need to be used in a highly integrated and synergistic manner.

Fundamentals of  Nanoscale Film Analysis (Hardcover, 2007 ed.): Terry L. Alford, L.C. Feldman, James W. Mayer Fundamentals of Nanoscale Film Analysis (Hardcover, 2007 ed.)
Terry L. Alford, L.C. Feldman, James W. Mayer
R2,941 Discovery Miles 29 410 Ships in 12 - 19 working days

From materials science to integrated circuit development, much of modern technology is moving from the microscale toward the nanoscale. This book focuses on the fundamental physics underlying innovative techniques for analyzing surfaces and near-surfaces. New analytical techniques have emerged to meet these technological requirements, all based on a few processes that govern the interactions of particles and radiation with matter. This book addresses the fundamentals and application of these processes, from thin films to field effect transistors.

Mass Spectrometry and Genomic Analysis (Hardcover, 2001 ed.): J. N. Housby Mass Spectrometry and Genomic Analysis (Hardcover, 2001 ed.)
J. N. Housby
R2,967 Discovery Miles 29 670 Ships in 10 - 15 working days

76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Muller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3."

In-Vitro Diagnostic Devices - Introduction to Current Point-of-Care Diagnostic Devices (Hardcover, 1st ed. 2016): Chao-Min... In-Vitro Diagnostic Devices - Introduction to Current Point-of-Care Diagnostic Devices (Hardcover, 1st ed. 2016)
Chao-Min Cheng, Chen-Meng Kuan, Chien-Fu Chen
R2,873 Discovery Miles 28 730 Ships in 10 - 15 working days

Addressing the origin, current status, and future development of point-of-care diagnostics, and serving to integrate knowledge and tools from Analytical Chemistry, Bioengineering, Biomaterials, and Nanotechnology, this book focusses on addressing the collective and combined needs of industry and academia (including medical schools) to effectively conduct interdisciplinary research. In addition to summarizing and detailing developed diagnostic devices, this book will attempt to point out the possible future trends of development for point-of-care diagnostics using both scientifically based research and practical engineering needs with the aim to help novices comprehensively understand the development of point-of-care diagnostics. This includes demonstrating several common but critical principles and mechanisms used in point-of-care diagnostics that address practical needs (e.g., disease or healthcare monitoring) using two well-developed examples so far: 1) blood glucose meters (via electrochemistry); and, 2) pregnancy tests (via lateral flow assay). Readers of this book will come to fully comprehend how to develop point-of-care diagnostics devices, and will be inspired to contribute to a critical global cause - the development of inexpensive, effective, and portable in vitro diagnostics tools (for any purpose) that can be used either at home or in resource limited areas.

Handbook of Analytical Science and Instrumentation: Volume II (Hardcover): Sherley Benson Handbook of Analytical Science and Instrumentation: Volume II (Hardcover)
Sherley Benson
R3,144 Discovery Miles 31 440 Ships in 12 - 19 working days
Liquid Crystal Display Drivers - Techniques and Circuits (Hardcover, 2009 ed.): David J.R. Cristaldi, Salvatore Pennisi,... Liquid Crystal Display Drivers - Techniques and Circuits (Hardcover, 2009 ed.)
David J.R. Cristaldi, Salvatore Pennisi, Francesco Pulvirenti
R2,922 Discovery Miles 29 220 Ships in 10 - 15 working days

Liquid Crystal Display Drivers deals with Liquid Crystal Displays from the electronic engineering point of view and is the first expressively focused on their driving circuits. After introducing the physical-chemical properties of the LC substances, their evolution and application to LCDs, the book converges to the examination and in-depth explanation of those reliable techniques, architectures, and design solutions amenable to efficiently design drivers for passive-matrix and active-matrix LCDs, both for small size and large size panels. Practical approaches regularly adopted for mass production but also emerging ones are discussed. The topics treated have in many cases general validity and found application also in alternative display technologies (OLEDs, Electrophoretic Displays, etc.).

Nuclear Magnetic Resonance - Volume 31 (Hardcover, Edition.): Cynthia J. Jameson, Hiroyuki Fukui, Krystyna Kamienska-Trela, A.... Nuclear Magnetic Resonance - Volume 31 (Hardcover, Edition.)
Cynthia J. Jameson, Hiroyuki Fukui, Krystyna Kamienska-Trela, A. E. Aliev, C.L. Khetrapal, …
R10,945 Discovery Miles 109 450 Ships in 10 - 15 working days

As a spectroscopic method, Nuclear Magnetic Resonance (NMR) has seen spectacular growth over the past two decades, both as a technique and in its applications. Today the applications of NMR span a wide range of scientific disciplines, from physics to biology to medicine. Each volume of Nuclear Magnetic Resonance comprises a combination of annual and biennial reports which together provide comprehensive of the literature on this topic. This Specialist Periodical Report reflects the growing volume of published work involving NMR techniques and applications, in particular NMR of natural macromolecules which is covered in two reports: "NMR of Proteins and Acids" and "NMR of Carbohydrates, Lipids and Membranes". For those wanting to become rapidly acquainted with specific areas of NMR, this title provides unrivalled scope of coverage. Seasoned practitioners of NMR will find this an in valuable source of current methods and applications. Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject areas, the series creates a unique service for the active research chemist, with regular, in-depth accounts of progress in particular fields of chemistry. Subject coverage within different volumes of a given title is similar and publication is on an annual or biennial basis.

Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobotics (Hardcover, 1st ed.... Investigations of Cellular and Molecular Biophysical Properties by Atomic Force Microscopy Nanorobotics (Hardcover, 1st ed. 2018)
Mili
R3,444 Discovery Miles 34 440 Ships in 12 - 19 working days

This book presents methodological and application research in detecting cellular and molecular biophysical properties based on atomic force microscopy (AFM) nanorobotics. Series methods for in situ label-free visualizing and quantifying the multiple physical properties of single cells and single molecules were developed, including immobilization strategies for observing fine structures of living cells, measurements of single-cell mechanics, force recognition of molecular interactions, and mapping protein organizations on cell surface. The biomedical applications of these methods in clinical lymphoma treatments were explored in detail, including primary sample preparation, cancer cell recognition, AFM detection and data analysis. Future directions about the biomedical applications of AFM are also given.

Rethinking Polyester Polyurethanes - Algae Based Renewable, Sustainable, Biodegradable and Recyclable Materials (Paperback):... Rethinking Polyester Polyurethanes - Algae Based Renewable, Sustainable, Biodegradable and Recyclable Materials (Paperback)
Robert S. Pomeroy
R3,447 R3,126 Discovery Miles 31 260 Save R321 (9%) Ships in 12 - 19 working days

Rethinking Polyester Polyurethanes: Algae Based Renewable, Sustainable, Biodegradable and Recyclable Materials explains how and why bio-based materials, specifically algae, will change the polymer industry. The book provides background on algae, polyurethanes (PUs), and their everyday use. It covers the biology and chemistry behind how and why these materials are engineered to be both biodegradable and, through the process of depolymerization, fully recyclable. Chapters cover Re-evaluating the Sources, Redefining the Analytics, Reformulating Polyester Polyurethanes, and The Future. The latter part of the book describes the landscape of bio-based materials, the eco-consumer, and insights into the industry problem of "greenwashing." This book is a valuable resource for industry professionals who use polyurethanes in the supply chain for manufactured products, those in sales and marketing or regulatory roles who wish to better understand the unique advantages of bio-based materials, and researchers studying R&D of biobased polyurethanes or remediation of microplastics pollution on land and in bodies of fresh and saltwater worldwide.

Elements of Molecular Spectroscopy (Hardcover): P.S. Sindhu Elements of Molecular Spectroscopy (Hardcover)
P.S. Sindhu
R1,074 Discovery Miles 10 740 Ships in 12 - 19 working days

Spectroscopy in this book has been made easier and attractive by adopting a non-mathematical pictorial path. The essential feature of this approach involves use of visual images at the molecular level. Further, to keep the reader involved, every chapter has a large number of solved problems. This approach brings molecular spectroscopy within the comprehension of the average reader. Application of molecular spectroscopy in determining molecular structure and the study of intermolecular interactions have also been discussed. A chapter on the applications of molecular spectroscopy has been prepared from the latest research work where molecular spectroscopy has been used as a tool to the research problems. This will give an idea as to how wide and divergent spectroscopy has its applications. Again, the approach intends to develop reasoning ability to the maximum.

Spectroscopy Essentials: Volume II (Hardcover): Allegra Smith Spectroscopy Essentials: Volume II (Hardcover)
Allegra Smith
R3,143 Discovery Miles 31 430 Ships in 12 - 19 working days
Dynamics of the Pantanal Wetland in South America (Hardcover, 1st ed. 2016): Ivan Bergier, Mario Luis Assine Dynamics of the Pantanal Wetland in South America (Hardcover, 1st ed. 2016)
Ivan Bergier, Mario Luis Assine
R6,500 Discovery Miles 65 000 Ships in 10 - 15 working days

This book provides readers with in-depth insights into the changes in the Pantanal wetland from its formation to the actual and likely future states. It reveals that today's Pantanal is an evolutionary consequence of geological, ecological and, more recently, man-made events taking place at distinct space-time intervals. Topics include geotectonics and sun-earth interactions, which largely dictate the rate of drastic changes that eventually disrupt ecological stability and radically rebuild the regional landscape. Furthermore, the biota-climate system is discussed as a major driver reshaping the ecohydrology functioning of the landscape on an intermediate timescale. Also covered are major changes in the landscape ecohydrology and biodiversity due to recent land-use and climate changes induced by humankind in the Anthropocene. The ability to recognize how those temporal scales impact the Pantanal wetland provides the opportunity for wise management approaches and the sustainable development of the region.

Advanced Research in Chromatography (Hardcover): Carlos Dayton Advanced Research in Chromatography (Hardcover)
Carlos Dayton
R3,438 R3,108 Discovery Miles 31 080 Save R330 (10%) Ships in 10 - 15 working days
Spectroscopy Essentials: Volume I (Hardcover): Allegra Smith Spectroscopy Essentials: Volume I (Hardcover)
Allegra Smith
R3,143 Discovery Miles 31 430 Ships in 12 - 19 working days
Gel-Free Proteomics - Methods and Protocols (Hardcover, 2011 ed.): Kris Gevaert, Joel Vandekerckhove Gel-Free Proteomics - Methods and Protocols (Hardcover, 2011 ed.)
Kris Gevaert, Joel Vandekerckhove
R2,979 Discovery Miles 29 790 Ships in 10 - 15 working days

Proteomics by means of mass spectrometry has rapidly changed the way that we analyze proteomes. "Gel-Free Proteomics: Methods and Protocols" addresses contemporary methods for gel-free proteome research with a special focus on differential analysis and protein modifications. Divided into twenty-five chapters, this detailed volume meticulously describes vital procedures needed to perform gel-free proteomics, ranging from sample preparation, isotope labeling for differential proteomics, enrichment technologies for modified proteins and peptides, and bioinformatics. Written in the successful "Methods in Molecular Biology " series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, "Gel-Free Proteomics: Methods and Protocols" serves as a timely resource for both professionals and novices pursing research in this critical field."

Advanced Photon Counting - Applications, Methods, Instrumentation (Hardcover, 2015 ed.): Peter Kapusta, Michael Wahl, Rainer... Advanced Photon Counting - Applications, Methods, Instrumentation (Hardcover, 2015 ed.)
Peter Kapusta, Michael Wahl, Rainer Erdmann
R10,915 Discovery Miles 109 150 Ships in 12 - 19 working days

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a "fingerprint" for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Foerster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Properties and Applications of Nanocrystalline Alloys from Amorphous Precursors - Proceedings of the NATO Advanced Research... Properties and Applications of Nanocrystalline Alloys from Amorphous Precursors - Proceedings of the NATO Advanced Research Workshop on Properties and Applications of Nanocrystalline Alloys from Amorphous Precursors, Budmerice, Slovak Republic, from 9 - 15 June 2003. (Hardcover, 2005 ed.)
Bogdan Idzikowski, Peter Svec, Marcel Miglierini
R8,554 Discovery Miles 85 540 Ships in 10 - 15 working days

Metallic (magnetic and non-magnetic) nanocrystalline materials have been known for over ten years but only recent developments in the research into those complex alloys and their metastable amorphous precursors have created a need to summarize the most important accomplishments in the field. This book is a collection of articles on various aspects of metallic nanocrystalline materials, and an attempt to address this above need.

The main focus of the papers is on the new issues that emerge in the studies of nanocrystalline materials, and, in particular, on (i) new compositions of the alloys, (ii) properties of conventional nanocrystalline materials, (iii) modeling and simulations, (iv) preparation methods, (v) experimental techniques of measurements, and (vi) different modern applications. Interesting phenomena of the physics of nanocrystalline materials are a consequence of the effects induced by the nanocrystalline structure. They include interface physics, the influence of the grain boundaries, the averaging of magnetic anisotropy by exchange interactions, the decrease in exchange length, and the existence of a minimum two-phase structure at the atomic scale.

Attention is also paid to the special character of the local atomic ordering and to the corresponding interatomic bonding as well as to anomalies and particularities of electron density distributions, and to the formation of metastable, nanocrystalline (or quasi-crystalline) phases built from exceptionally small grains with special properties. Another important focus of attention are new classes of materials which are not based on new compositions, but rather on the original and special crystalline structure in thenanoscale.

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