Therapeutic protein drug products provides a comprehensive overview of therapeutic protein drug products, with an emphasis on formulation beginning in the laboratory, followed by manufacturing and administration in the clinic. A list of many commercial therapeutic drug products are described and include the product name, dosages, active concentration, buffer, excipients, Ph, container type and route of administration. The laboratory formulation sections focus on the most common buffers, excipients, and Ph ranges that are commonly tested in addition to systematic approaches. A brief section on biophysical and analytical analysis is also provided. Properties of therapeutic protein formulations are described and include opalescence, phase separation, color, and subvisible particles. An emphasis is placed on material and process testing to ensure success during manufacturing. The drug product manufacturing process, which includes the process of compounding to filling, is also covered. Methods of delivery in the clinic are addressed, as well as delivery strategies. Finally, a perspective on the regulatory requirements for therapeutic protein formulations is discussed.
Provides a list and description of commercially available therapeutic drug products and their formulationsA comprehensive and practical overview of protein formulation in the laboratory, manufacturing, and the clinicDiscusses recent topics including high protein concentration, phase separation, opalescence, and subvisible particles

For over thirty years Susan Wolf has been writing about moral and nonmoral values and the relation between them. This volume collects Wolf's most important essays on the topics of morality, love, and meaning, ranging from her classic essay "Moral Saints" to her most recent "The Importance of Love." Wolf's essays warn us against the common tendency to classify values in terms of a dichotomy that contrasts the personal, self-interested, or egoistic with the impersonal, altruistic or moral. On Wolf's view, this tendency ignores or distorts the significance of such values as love, beauty, and truth, and neglects the importance of meaningfulness as a dimension of the good life. These essays show us how a self-conscious recognition of the variety of values leads to new understandings of the point, the content, and the limits of morality and to new ways of thinking about happiness and well-being.

This volume of "Methods in Cell Biology" is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.
* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* This volume concentrates on Disease Models and Chemical Screens

Despite the massive scale of global inequalities, until recently few political philosophers or bioethicists addressed their ethical implications. Questions of justice were thought to be primarily internal to the nation state. Over the last decade or so, there has been an explosion of interest in the philosophical issues surrounding global justice. These issues are of direct relevance to bioethics. The links between poverty and health imply that we cannot separate questions of global health from questions about fair distribution of global resources and the institutions governing the world order. Similarly, as increasing numbers of medical trials are conducted in the developing world, researchers and their sponsors have to confront the special problems of doing research in an unjust world, with corresponding obligations to correct injustice and avoid exploitation. This book presents a collection of original essays by leading thinkers in political theory, philosophy, and bioethics. They address the key issues concerning global justice and bioethics from two perspectives. The first is ideal theory, which is concerned with the social institutions that would regulate a just world. What is the relationship between human rights and the provision of health care? How, if at all, should a global order distinguish between obligations to compatriots and others? The second perspective is from non-ideal theory, which governs how people should behave in the unjust world in which we actually find ourselves. What sort of medical care should actual researchers working in impoverished countries offer their subjects? What should NGOs do in the face of cultural practices with which they deem unethical? If coordinated international action will not happen, what ought individual states to do? These questions have more than theoretical interest; their answers are of direct practical import for policymakers, researchers, advocates, NGOs, scholars, and others. This book is the first collection to comprehensively address the intersection of global justice and bioethical dilemmas.

This MIE volume provides laboratory techniques that aim to predict the structure of a protein which can have tremendous implications ranging from drug design, to cellular pathways and their dynamics, to viral entry into cells.
Expert researchers introduce the most advanced technologies and techniques in protein structure and folding
Includes techniques on tiling assays.

"International Review of Cell & Molecular Biology" presents current advances and comprehensive reviews in cell biology--both plant and animal. Articles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth.

* Authored by some of the foremost scientists in the field * Provides up-to-date information and directions for future research * Valuable reference material for advanced undergraduates, graduate students and professional scientists

Published since 1953, "Advances in Virus Research" covers a diverse range of in-depth reviews providing a valuable overview of the current field of virology.
The impact factor for 2008 is 4.886, placing it 4th in the highly competitive category of virology.

* Contributions from leading authorities
* Informs and updates on all the latest developments in the field

In the last fifteen years, there has been significant interest in studying the brain structures involved in moral judgments using novel techniques from neuroscience such as functional magnetic resonance imaging (fMRI). Many people, including a number of philosophers, believe that results from neuroscience have the potential to settle seemingly intractable debates concerning the nature, practice, and reliability of moral judgments. This has led to a flurry of scientific and philosophical activities, resulting in the rapid growth of the new field of moral neuroscience. There is now a vast array of ongoing scientific research devoted towards understanding the neural correlates of moral judgments, accompanied by a large philosophical literature aimed at interpreting and examining the methodology and the results of this research. This is the first volume to take stock of fifteen years of research of this fast-growing field of moral neuroscience and to recommend future directions for research. It features the most up-to-date research in this area, and it presents a wide variety of perspectives on this topic.

Written by an immunologist, this book traces the concept of immunity from ancient times up to the present day, examining how changing concepts and technologies have affected the course of the science. It shows how the personalities of scientists and even political and social factors influenced both theory and practice in the field. With fascinating stories of scientific disputes and shifting scientific trends, each chapter examines an important facet of this discipline that has been so central to the development of modern biomedicine. With its biographical dictionary of important scientists and its lists of significant discoveries and books, this volume will provide the most complete historical reference in the field.
Written in an elegant style by long-time practicing immunologist
Discusses the changing theories and technologies that guided the field
Tells of the exciting disputes among prominent scientists
Lists all the important discoveries and books in the field
Explains in detail the many Nobel prize-winning contributions of immunologists"

Since its inception in 1945, this serial has provided critical and integrating articles written by research specialists that integrate industrial, analytical, and technological aspects of biochemistry, organic chemistry, and instrumentation methodology in the study of carbohydrates. The articles provide a definitive interpretation of the current status and future trends in carbohydrate chemistry and biochemistry.

The book is based on data collected during the past 10 years by Zackenberg Ecological Research Operations (ZERO) at Zackenberg Research Station in northeast Greenland. This volume covers the function of Arctic ecosystems based on the most comprehensive long-term data set in the world from a well-defined Arctic ecosystem. Editors offer a comprehensive and authoritative analysis of how climate variability is influencing an Arctic ecosystem and how the Arctic ecosystems have inherent feedback mechanisms interacting with climate variability or change.
* The latest research on the functioning of Arctic ecosystems
* Supplements current books on Arctic climate impact assessment as a case study for ecological specialists
* Discusses the complex perpetuating effects on Earth
* Vital information on modeling ecosystem responses to understand future climates

Levels of mycotoxin contamination in agricultural commodities are regulated in more than 100 countries, and exposure to these naturally occurring toxins presents serious risks to the health of humans and animals with negative impacts to commodity values. This symposium series book presents significant scientific developments in the multifaceted approach to reducing exposure to these naturally occurring toxins. A broad-spectrum of subject matter of the multidisciplinary field of mycotoxin research is conveniently compiled in this single volume, and general themes include prevention, control, exposure, molecular biology, biosynthesis, analytical methodology, and emerging techniques. The book opens with an overview of prevention of mycotoxin production by means of biological control and human exposure to contaminated foods, including tofu, apples, figs, and a broad range of fruits.
The second section focuses on molecular biological approaches to control mycotoxin exposure. Topics covered include genomic approaches, detoxification, structure-activity studies of biosynthetic precursors, and mycotoxin biosynthesis. The success of these strategies is supported by the reduction of mycotoxin occurrence and incidence in foods. Accurate estimates of mycotoxin concentration in foods are important for food safety and wholesomeness.
The final section covers analytical approaches, including sampling plans for foods and dietary supplements, extraction efficiency of mycotoxins from naturally contaminated grains, traditional detection methods such as chromatography/mass spectrometry, and emerging techniques such as the use of molecularly imprinted polymers for purification and isolation and cyclodextrins for fluorescent enhancement of several mycotoxins.

This is the fourth edition of an established and successful reference for plant scientists. The author has taken into consideration extensive reviews performed by colleagues and students who have touted this book as the ultimate reference for research and learning. The original structure and philosophy of the book continue in this new edition, providing a genuine synthesis of modern physicochemical and physiological thinking, while entirely updating the detailed content. Key concepts in plant physiology are developed with the use of chemistry, physics, and mathematics fundamentals.
The figures and illustrations have been improved and the list of references has been expanded to reflect the author's continuing commitment to providing the most valuable learning tool in the field. This revision will ensure the reputation of Park Nobel's work as a leader in the field.
* More than 40% new coverage
* Incorporates student-recommended changes from the previous edition
* Five brand new equations and four new tables, with updates to 24 equations and six tables
* 30 new figures added with more than three-quarters of figures and legends improved
* Organized so that a student has easy access to locate any biophysical phenomenon in which he or she is interested
* Per-chapter key equation tables
* Problems with solutions presented in the back of the book
* Appendices with conversion factors, constants/coefficients, abbreviations and symbols

Now published by "Academic Press" and revised from the author's previous "Five Kingdoms, Third Edition", this extraordinary, all inclusive catalogue of the world's living organisms describes the diversity of the major groups, or phyla, of nature's most inclusive taxa. Developed after consultation with specialists, this modern classification scheme is consistent both with the fossil record and with recent molecular, morphological and metabolic data. Generously illustrated, now in full color, "Kingdoms and Domains" is remarkably easy to read. It accesses the full range of life forms that still inhabit our planet and logically and explicitly classifies them according to their evolutionary relationships. Definitive characteristics of each phylum are professionally described in ways that, unlike most scientific literature, profoundly respect the needs of educators, students and nature lovers. This work is meant to be of interest to all evolutionists as well as to conservationists, ecologists, genomicists, geographers, microbiologists, museum curators, oceanographers, paleontologists and, especially nature lovers whether artists, gardeners or environmental activists. "Kingdoms and Domains" is a unique and indispensable reference for anyone intrigued by a planetary phenomenon: the spectacular diversity of life, both microscopic and macroscopic, as we know it only on Earth today. This work also carries: new foreword by Edward O. Wilson; the latest concepts of molecular systematics, symbiogenesis, and the evolutionary importance of microbes; newly expanded chapter openings that define each kingdom and place its members in context in geological time and ecological space; definitions of terms in the glossary and throughout the book; ecostrips, illustrations that place organisms in their most likely environments such as deep sea vents, tropical forests, deserts or hot sulfur springs; and, a new table that compares features of the most inclusive taxa. This book shows an application of a logical, authoritative, inclusive and coherent overall classification scheme based on evolutionary principles.

Published since 1959, "Advances in Applied Microbiology" continues to be one of the most widely read and authoritative review sources in microbiology.
The series contains comprehensive reviews of the most current research in applied microbiology. Recent areas covered include bacterial diversity in the human gut, protozoan grazing of freshwater biofilms, metals in yeast fermentation processes and the interpretation of host-pathogen dialogue through microarrays.
Eclectic volumes are supplemented by thematic volumes on various topics, including Archaea and sick building syndrome. Impact factor for 2007: 1.821.
* Contributions from leading authorities and industry experts
* Informs and updates on all the latest developments in the field
* Reference and guide for scientists and specialists involved in advancements in applied microbiology

The third volume in the AOCS Monograph Series on Oilseeds presents a unique blend of information focusing on edible oils. These oils contain either unique flavor components that have lead to their being considered gourmet oils, or contain unique health-promoting chemical components. Each chapter covers processing, edible and non-edible applications, lipids, and the health benefits related to each type of oil.

This book provides a foundation for understanding the fundamentals of biomedical informatics, which deals with the storage, retrieval, and use of biomedical data for biological problem solving and medical decision making. It covers the application of these principles to the three main biomedical domains of basic biology, clinical medicine, and public health. The author offers a coherent summary, focusing on the three core concept areas of biomedical data and knowledge representation, biomedical information access, biomedical decision making, and information and technology use in biomedical contexts.
* Develops principles and methods for representing biomedical data, using information in context and in decision making, and accessing information to assist the medical community in using data to its full potential
* Provides a series of principles for expressing biomedical data and ideas in a computable form to integrate biological, clinical, and public health applications
* Includes a discussion of user interfaces, interactive graphics, and knowledge resources and reference material on programming languages to provide medical informatics programmers with the technical tools to develop systems

"Advances in Planar Lipid Bilayers and Liposomes, " Volume 8, continues to include invited chapters on a broad range of topics, covering both main arrangements of the reconstituted system, namely planar lipid bilayers and spherical liposomes. The invited authors present the latest results in this exciting multidisciplinary field of their own research group.
Many of the contributors working in both fields over many decades were in close collaboration with the late Prof. H. Ti Tien, the founding editor of this book series. There are also chapters written by some of the younger generation of scientists included in this series. This volume keeps in mind the broader goal with both systems, planar lipid bilayers and spherical liposomes, which is the further development of this interdisciplinary field worldwide.
* Incorporates contributions from newcomers and established and experienced researchers
* Explores the planar lipid bilayer systems and spherical liposomes from both theoretical and experimental perspectives
* Serves as an indispensable source of information for new scientists

The book is based on data collected during the past ten years by Zackenberg Ecological Research Operations (ZERO) at Zackenberg Research Station in Northeast Greenland. This volume covers the function of Arctic ecosystems based on the most comprehensive long-term data set in the world from a well-defined Arctic ecosystem. Editors offer a comprehensive and authoritative analysis of how climate variability is influencing an Arctic ecosystem and how the Arctic ecosystems have inherent feedback mechanisms interacting with climate variability or change.
* The latest research on the functioning of Arctic ecosystems
* Supplement current books on arctic climate impact assessment as a case study for ecological specialists
* Discusses the complex perpetuating effects on Earth
* Vital information on modelling ecosystem responses to understand future climates

Phospholipid technology and applications is an essential reference for technologists developing food and cosmetics products, scientists researching phospholipids in biological and food systems, technologists in fats and oils refining, and scientists developing drugs and drug delivery systems and carriers.
The major source of phospholipids is the lecithin recovered during degumming of vegetable oils, particularly soybean oil. This crude material finds uses in its own right but can be purified through a series of processes which eventually lead to individual phospholipid classes such as phosphatidylcholines.
It is widely accepted that oil and water do not mix but there are several areas in science and technology where these two distinct phases must coexist in stable emulsions. This is achieved by admixture of amphiphilic molecules of which the phospholipids are important natural examples. Today, phospholipids find many uses in the food industry and in other industries which exploit the amphiphilic nature of these compounds. Further, there are now important procedures by which their amphiphilicity can be optimized for different uses.
The early chapters in this book are devoted to the more common glycerol-based phospholipids and cover their structure, source, composition, modification by chemical and enzymatic methods, their physical, chemical, and nutritional properties, and their major uses. The final chapter is devoted to another kind of phospholipid, the sphingolipids, in which there is a growing interest.

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and analyzing the results. The critical tricks for obtaining a good FRET image and precisely quantitating the signals from living specimens at the nanomolecular level are explained. Valuable information about the preparation of biological samples used for FRET image analysis is also provided.
The methods covered include different types of microscopy systems and detectors (wide-field, confocal, multi-photon) as well as specialized techniques such as photobleaching FRET, FLIM-FRET microscopy, spectral imaging FRET, single molecule FRET, and time and image correlation spectroscopy. Starting from the basics, the chapters guide readers through the choice of probes to be used for FRET experiments and the selection of the most suitable experimental approaches to address specific biological questions. Up-to-date, consistently organized, and well-illustrated, this unique book will be welcomed by all researchers who wish to use FRET microscopy and spectroscopy techniques.

Advances in Planar Lipid Bilayers and Liposomes, Volume 6, continues to include invited chapters on a broad range of topics, covering both main arrangements of the reconstituted system, namely planar lipid bilayers and spherical liposomes. The invited authors present the latest results in this exciting multidisciplinary field of their own research group.
Many of the contributors working in both fields over many decades were in close collaboration with the late Prof. H. Ti Tien, the founding editor of this book series. There are also chapters written by some of the younger generation of scientists included in this series. This volume keeps in mind the broader goal with both systems, planar lipid bilayers and spherical liposomes, which is the
further development of this interdisciplinary field worldwide.
* Contributions from newcomers and established and experienced researchers
* Exploring theoretically and experimentally the planar lipid bilayer systems and spherical liposomes
* This volume is dedicated to mark the Bilayer Lipid Membranes 45th anniversary

This textbook provides a thorough and comprehensive overview of the human brain and spinal cord for medical and graduate students as well as residents in the clinical neurosciences. Standing on the shoulders of training from outstanding scientist-teacher mentors and based on more than 30 years of experience teaching about the brain and spinal cord to medical and graduate students, this single authored text presents everything the reader would need as they begin their study of the nervous system. At the same time the experienced neuroscientist will find much useful and valuable information in these pages that is based almost exclusively on studies in experimental primates and observations in humans. Every effort has been made to present the complexities of the nervous system as simply and clearly as possible. The careful reader will discover a clarity and depth of coverage that makes the reading both instructional and enjoyable. Topics are presented logically and the text in an easy-to-read style. The accompanying line drawings emphasize important concepts in a clear and uncluttered manner.
Topics presented:
- Neurons, glial cells, degeneration, regeneration, axonal transport
- Review of the development of the human nervous system
- Overview of the anatomy of the spinal cord, brain stem and forebrain
- General sensory paths (pain, temperature, touch, pressure, proprioception)
- Special sensory systems (auditory, vestibular, visual, olfactory and gustatory)
- Eye movements and visual reflexes
- Comprehensive presentation of the regions involved in motor activity including the clinical manifestation of injuries to these motor areas
- Limbic system, hypothalamusand the autonomic nervous system
- Lobes of the brain, clinically important cortical areas and the results of lesions in these areas
- Blood supply to the spinal cord, brain stem, and brain including classical brain stem syndromes
- The meninges and the ventricular system
- Numerous helpful clinical correlations that emphasize the practical application of basic anatomical information.
* Presents the complexities of the nervous system as simply and clearly as possible
* Written with a clarity and depth of coverage that makes the reading both instructional and enjoyable
* Includes numerous illustrations emphasizing important concepts

Africa has been and continues to be a significant source of medicinal and aromatic plants and botanicals to the world's food, drug, herb and dietary supplement market, and in the past decade numerous African plant materials have established a strong international market presence. This book provides an excellent opportunity to delve into the current and future contributions that African plants can and will continue to make both internal to Africa and on the global stage. This book expertly covers various medicinal plants of African origin and the some of the latest basic and clinical research supporting their ongoing and potential uses in self-care and healthcare. This work also examines various issues and trends in medicinal plants from their uses in Traditional Medicine and ethnobotany, to our modern understanding of the plants chemistry and pharmacognosy, natural products chemistry and applications of medicinal plants, quality control, and models of benefit sharing.

In July 2009, many experts in the mathematical modeling of biological sciences gathered in Les Houches for a 4-week summer school on the mechanics and physics of biological systems. The goal of the school was to present to students and researchers an integrated view of new trends and challenges in physical and mathematical aspects of biomechanics. While the scope for such a topic is very wide, they focused on problems where solid and fluid mechanics play a central role. The school covered both the general mathematical theory of mechanical biology in the context of continuum mechanics but also the specific modeling of particular systems in the biology of the cell, plants, microbes, and in physiology.
These lecture notes are organized (as was the school) around five different main topics all connected by the common theme of continuum modeling for biological systems: Bio-fluidics, Bio-gels, Bio-mechanics, Bio-membranes, and Morphogenesis. These notes are not meant as a journal review of the topic but rather as a gentle tutorial introduction to the readers who want to understand the basic problematic in modeling biological systems from a mechanics perspective.