Oxidative stress is the result of an imbalance in pro-oxidant/antioxidant homeostasis that leads to the generation of toxic reactive oxygen species. Brain cells are continuously exposed to reactive oxygen species generated by oxidative metabolism, and in certain pathological conditions defense mechanisms against oxygen radicals may be weakened and/or overwhelmed. DNA is a potential target for oxidative damage, and genomic damage can contribute to neuropathogenesis. It is important therefore to identify tools for the quantitative analysis of DNA damage in models on neurological disorders. This book presents detailed information on various neurodegenerative disorders and their connection with oxidative stress. This information will provide clinicians with directions to treat these disorders with appropriate therapy and is also of vital importance for the drug industries for the design of new drugs for treatment of degenerative disorders.
* Contains the latest information on the subject of neurodegenerative disorders
* Reflects on various factors involved in degeneration and gives suggestions for how to tackle these problems

Volume forty-two in the internationally acclaimed Advances in Clinical Chemistry series, contains chapters submitted from leading experts from academia and clinical laboratory science. Authors are from a diverse field of clinical chemistry disciplines and diagnostics ranging from basic biochemical exploration to cutting edge microarray technology. In keeping with the tradition of the series, this volume emphasizes novel laboratory advances with application not only to both clinical laboratory diagnostics, but as well as practical basic science studies.
This volume of Advances in Clinical Chemistry is an indispensable resource and practical guide for twenty-first century practitioners of clinical chemistry, molecular diagnostics, pathology, and clinical laboratory sciences in general.

This fourth edition volume expands on the previous editions by presenting readers with new and emerging methodologies in cytometry. The chapters in this book cover various topics such as quantifying surface and intracellular protein biomarkers, intracellular cytokine staining, apoptosis analysis, cell cycle analysis, tracking cell proliferation, and monitoring protein-protein interactions using FRET. Some developing methodologies that explored are mass cytometry, vesicle flow cytometry, time-resolved flow cytometry, and real-time label-free deformability cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Flow Cytometry Protocols, Fourth Edition researchers and scientists who are interested in continuing or expanding their knowledge of flow cytometry.

Leading academic and industrial investigators surveys the world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification, and quantification of proteins, and for studies of protein-protein interactions. The protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Molecular Diagnostics covers current molecular biological techniques used to identify the underlying molecular defects in inherited disease. Although an increasing number of laboratories, both academic and private are moving in that direction, there are only a few books in the existing literature, and they deal only partly with diagnosis at the molecular level. Each chapter includes the principle and a brief description of the technique, followed by exmples from the authors' own epertise. Contributors are well-known experts in their field, and derive from a variety of disciplines, to ensure breadth and depth of coverage.
* Examines widely used molecular biology techniques to screen for genetic defects causing inherited disorders
* Includes state-of-the-art techniques for the detection of the underlying genetic heterogeneity leading to inherited disorders
* Identification of genetically modified organisms (GMO's)
* Forensic analysis and every-day issues in a diagnostic laboratory
* Discusses ethics, genetic counselling and quality management

A compendium of the latest published diagnostic criteria for a diverse group of neurological conditions. The criteria cover a wide spectrum of neurological conditions whose diagnosis does not depend solely on histopathology (e.g., brain tumors), including cerebrovascular diseases, dementias and behavioral disorders, demyelinating disorders, and disorders of consciousness and brain death. Standardized criteria are also provided for epilepsy, genetic syndromes, headache, immune-based disorders, infectious diseases, movement disorders, neuromuscular disorders, and sleep disorders. A dazzling array of tables summarizes useful information on both common and uncommon neurological conditions, including many genetic and pediatric neurological disorders. Innovative and authoritative, Diagnostic Criteria in Neurology begins to do for neurology what the DSM (Diagnostic and Statistical Manual) did for psychiatry.

A detailed description of a new approach to perceptual analysis and processing of medical images is given. Instead of traditional pattern recognition a new method of image analysis is presented, based on a syntactic description of the shapes selected on the image and graph-grammar parsing algorithms. This method of "Image Understanding" can be found as a model of mans' cognitive image understanding processes. The usefulness for the automatic understanding of the merit of medical images is demonstrated as well as the ability for giving useful diagnostic descriptions of the illnesses. As an application, the production of a content-based, automatically generated index for arranging and for searching medical images in multimedia medical databases is presented.

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.

The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known "Laboratory Techniques" series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies.
The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as well as ultrasensitive enzyme immunoassays.
"Ultrasensitive and Rapid Enzyme Immunoassay, " with its clear presentation and up-to-date information, will be essential to scientists in pharmaceutical companies and in applied research.

There is currently no detailed and comprehensive source of direct information from Chinese sources on the full scope of Traditional Chinese medicine diagnosis. This meticulous translation offers, for the first time in English, an authoritative and detailedcompilation of the principles and practice of TCM diagnostic methods and approaches. This translation has been carefully prepared and polished by translators in both China and the west, and has been edited thoroughly to maximize its accessibility by Western practitioners.Provides a single source for the widest variety of applied approaches and methods to come out in one volume on the topic of diagnosis Covers commonly seen clinical symptoms in separate chapters, detailing possible approaches to diagnosis and lines of exploration

This comprehensive text explains the diagnostic and optometric management procedures involved in the care of patients with binocular vision anomalies. It is a clinically oriented text that can be used by the student as well as the clinician. It provides thorough descriptions of the many binocular vision anomalies, describes the necessary testing procedures to correctly diagnose each disorder, and suggests the most appropriate management. Featuring many illustrations, photos and case studies, particularly on strabismus and incomitant deviations, it presents an orderly practical approach to diagnosis, differential diagnosis and treatment decision making. * Every topic area in binocular vision anomalies is included, from heterophoria and accommodative disorders to severe strabismic disorders. * Discusses not only the most common forms of treatment for a particular anomaly, but also discusses less known but deserving treatment alternatives, providing the reader with an understanding of the full range of treatment possibilities. * In each chapter, an anomaly is identified, then classified according to its significant symptoms and clinical findings. Once a diagnosis is certain, the appropriate forms of treatment are discussed. * Editors Rutstein and Daum are well known and respected in their field. They are scientists who have lectured extensively, have written many articles and have a wealth of clinical experience. * Organized to take the reader from the base to the tip of the "pyramid" of binocular vision. Sensory anomalies are discussed first, integrative anomalies next, and motor anomalies last. * An invaluable reference for both optometrists and ophthalmologists. * Features many references and case studies. * Contains approximately 309 illustrations and photographs, providing the reader with a clear, visual guide to the disorders and their management. * Features clinical pearls succinct bits of "clinical wisdom" that are boxed within the text for easy reference. * Outlines appear at the beginning of each chapter, giving the reader a quick overview of chapter content. * Glossary appears at the end of the book, defining key words that appear in the text.

"The Physical Lincoln Complete" combines two groundbreaking books -- "The Physical Lincoln" and "The Physical Lincoln Sourcebook" -- into one handy volume. It is the ultimate guide to the startling health history of Abraham Lincoln and his family. The first book, "The Physical Lincoln," offers a solution to one of the most enduring mysteries in American history: What made Abraham Lincoln so tall, thin, and less than attractive? What gave him his long limbs, large feet, high voice, odd lips, sluggish bowels, and astonishing joint flexibility? The never-before-proposed solution points to Lincoln's DNA and the rare genetic disorder called MEN2B. In addition to producing Lincoln's remarkable body shape, MEN2B gave him a sad-looking face that, for more than 150 years, has been consistently misinterpreted as depression. It tragically took his mother and three of his sons at early ages (Eddie, Willie, and Tad), and it was killing Lincoln in his last years. "The Physical Lincoln" upends the myth of a physically vibrant President, showing that, had he not been shot, Lincoln would have died from advanced cancer in less than a year, the result of MEN2B. Written in clear, non-technical language for the general reader, and using more than 180 illustrations, "The Physical Lincoln" offers fundamental new insights into Lincoln, and is the perfect book to stimulate a young person's interest in science and medicine. The second book, "The Physical Lincoln Sourcebook," organizes more than 5900 fully-referenced historical facts and three family trees into the fullest possible medical record for Lincoln and his family members. Nine special topics explore major issues such as Lincoln's smallpox, his gait, hisshooting, the possibility that he had syphilis, and the innumerable medical complaints of Mary Lincoln, his wife. Heavily annotated throughout, with an extensive bibliography and an on-line full-text index of the main sections, "The Physical Lincoln Sourcebook" is the definitive, comprehensive reference to all facets of Lincoln's anatomy and physiology. Scholars, Lincoln presenters, and anyone truly wishing to understand Abraham Lincoln will find it indispensable. See www.physical-lincoln.com for more information.

Medical Image Databases covers the new technologies of biomedical imaging databases and their applications in clinical services, education, and research. Authors were selected because they are doing cutting-edge basic or technology work in relevant areas. This was done to infuse each chapter with ideas from people actively investigating and developing medical image databases rather than simply review the existing literature. The authors have analyzed the literature and have expanded on their own research. They have also addressed several common threads within their generic topics. These include system architecture, standards, information retrieval, data modeling, image visualizations, query languages, telematics, data mining, and decision supports. The new ideas and results reported in this volume suggest new and better ways to develop imaging databases and possibly lead us to the next information infrastructure in biomedicine. Medical Image Databases is suitable as a textbook for a graduate-level course on biomedical imaging or medical image databases, and as a reference for researchers and practitioners in industry.

This volume contains the papers presented at the 14th International Conference on Information Processing in Medical Imaging. IPMI meetings have a a strong emphasis on the clinical relevance and validation of medical imaging. This book covers the whole spectrum: acquisition, tomographic reconstruction, registration, segmentation, knowledge-based analysis, display and image quality as well as several important applications. Several papers present significant advances in topics already discussed at previous meetings while others deal with new topics and methodology, opening new horizons in medical imaging. In addition to the 28 full-length papers, 30 short communications are included to sample the most current work in progress. Audience: An up-to-date and complete overview of ongoing research in medical imaging, beneficial to all physicists, computer scientists and physicians who wish to remain informed on state-of-the-art methodology in medical imaging.

In the past, many tumor marker laboratory tests have not been sensitive enough for the very early detection of cancer. However, many of them have nonetheless proved useful in monitoring therapy, following the course of the tumor, and predicting prog- nosis. Today, cancer may be viewed as a genetic disease with various specific chromo- somal and nucleotide aberrations, such as mutations, deletions, gene amplification, gene rearrangements, and translocations occurring during the transformation of a nor- mal cell into a malignant cell. The considerable advances in technology during the past several years have greatly enhanced our ability to detect human cancers in the very early stages of tumor forma- tion. These technologies include: (1) nucleotide molecular assays (genomics); (2) proteomics (multiplex protein measurements); (3) DNA microarrays; and (4) bio- informatics. Many of these technologies are already helping in the integration and use of multiple biomarkers for tumors. Although the individual biomarkers may reveal only limited information, the use of multiple biomarkers can help markedly elevate the diagnostic capabilities for early detection of tumors.

Addressing the origin, current status, and future development of point-of-care diagnostics, and serving to integrate knowledge and tools from Analytical Chemistry, Bioengineering, Biomaterials, and Nanotechnology, this book focusses on addressing the collective and combined needs of industry and academia (including medical schools) to effectively conduct interdisciplinary research. In addition to summarizing and detailing developed diagnostic devices, this book will attempt to point out the possible future trends of development for point-of-care diagnostics using both scientifically based research and practical engineering needs with the aim to help novices comprehensively understand the development of point-of-care diagnostics. This includes demonstrating several common but critical principles and mechanisms used in point-of-care diagnostics that address practical needs (e.g., disease or healthcare monitoring) using two well-developed examples so far: 1) blood glucose meters (via electrochemistry); and, 2) pregnancy tests (via lateral flow assay). Readers of this book will come to fully comprehend how to develop point-of-care diagnostics devices, and will be inspired to contribute to a critical global cause - the development of inexpensive, effective, and portable in vitro diagnostics tools (for any purpose) that can be used either at home or in resource limited areas.

With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular BiologyT series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users. Content Level Professional/practitioner

The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. The detection of parasites in clinical cases, companion and farm animals, and in the environment is relatively easy since many of them are visible to the naked eye, and those that are not are readily detected by light microscopy. Fungal infections can similarly be determined. Bacteria are somewhat harder to detect. Although their presence can frequently be detected by light microscopy, differential diagnosis, beyond their gross morphology, is almost always impossible. However, most bacterial pathogens can be cultured in the laboratory and can be accurately identified by combinations of a series of simple tests such as morphology, staining, antibiotic sensitivity, biochemical analyses, nutrient dependence, and phage sensitivity. Viruses, however, have proved much more difficult; their size and absolute dependence of the host cell for propagation have rendered useless the methods traditionally used for other microorganisms. Until the development of tissue culture in the middle of this century, diagnosis was entirely dependent on the skill and experience of the clinician. But this was an unreliable process since many of the common virus infections exhibit similar clinical symptoms, such as coryza, exanthema, vomiting, diarrhea, neuralgia, and lethargy. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.

This volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. Microchip Diagnostics: Methods and Protocols is divided into four sections describing the business aspects of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis based on mono or diphasic format, and highlighting recent contributions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Physics has been applied to medical diagnosis for very nearly 400 years, and has now become an essential element of medical practice. This book concentrates on the theoretical basis of the physics which supports diagnostic techniques in modern clinical practice. Arising out of over a decade of teaching a course on medical physics to third year undergraduate students, the book has been structured so that individuals with a non-physics background, such as medical students or practitioners, can also benefit.

As many as one in four adults in the workforce will suffer from psychiatric illness in a given year. Such illness can have serious consequences -- job loss, lawsuits, workplace violence yet the effects of mental health issues on job functioning are rarely covered in clinical training. In addition, clinicians are often asked to provide opinions on an employee s fitness for work or an evaluation for disability benefits, only to find themselves embroiled in complex legal and administrative conflicts. A unique collaboration between a renowned clinical professor of psychiatry and a noted legal expert, Evaluating Mental Health Disability in the Workplace approaches the topic from two distinct areas: the legal context and issues relevant to disability and disability-related evaluations, and the interplay of factors in the relationship between work and psychiatric illness. From this dual perspective, the authors advocate for higher professional standards ensuring that employers, evaluees, or third parties are provided with the most reliable information.

Key features of the book:

• A robust assessment model of psychological disability in the workplace
• Practice guidelines for conducting workplace mental health disability evaluations
• Legal and ethical aspects of employment evaluations, especially as they differ from clinical procedure
• Examination of the process of psychiatric disability development
• Issues specific to evaluations for Social Security, Workers Compensation, and other disability benefit programs
• Review of relevant administrative and case law.

As an introduction to these complex issues or for the further improvement of evaluation skills, Evaluating Mental Health Disability in the Workplace is a timely reference for psychiatrists, psychologists, forensic mental health specialists, and attorneys in this field."