Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.

As healthcare costs rise, so too do the costs of assessment instruments, critical tools for mental health professionals. While some traditional assessment instruments have become prohibitively expensive, as with many other fields, the Internet offers a host of more affordable and equitable alternative assessment tools at little or no cost. The pitfall of this alternative, thus far, has been the lack of vetting and quality assessment. "Assessing Common Mental Health and Addiction Issues With Free-Access Instruments" fills this gap by providing the first analysis and assessment of these tools, provided by some of the leading names in mental health assessment instruments. This resource identifies the most efficient free access instruments and provides summary information about administration, scoring, interpretation, psychometric integrity, and strengths and weaknesses. The book is organized around the most common broad range issues encountered by helping professionals, and whenever possible, a link to the instrument itself is provided. This is an essential text for all mental health professionals looking to expand the scope and range of their assessment instruments.

Accurately identify complex geometrical distortions of cardiac anatomy and make more informed decisions regarding diagnosis and treatment with Atlas of 3D Echocardiography. This new cardiology reference presents the authoritative, case-based, expert guidance you need to enhance your utilization and interpretation of this cutting-edge, dimensional diagnostic tool. Master the application of techniques to specific clinical situations with detailed case studies and discussions of challenging issues. See imaging findings as they appear in practice and discern subtle nuances with the aid of high-quality still images plus online videos. Reference the information you need quickly thanks to easy-to-follow, templated chapters, with an abundance of images and figures that facilitate visual learning. Take it with you anywhere! Access the full text, downloadable image library, videos, and more at www.expertconsult.com. Sharpen your interpretive and diagnostic skills in 3-D echo!

The aim of Hematologic Malignancies: Methods and Techniques is to review those methods most useful for the diagnosis and subsequent mana- ment of hematologic malignancies. The scope of coverage is intentionally broad, ranging from routine procedures to highly sophisticated methods not currently offered by most clinical laboratories. The latter methods were selected especially to bring into focus recent advances in molecular biology that, since they provide us with strong tools for assessing the outcome of upcoming therapeutic modalities intent on disease eradication, are expected to impact the future diagnosis and management of these diseases. Thus, the c- mon thread among all chapters is clinical relevance, whether sanctioned by past experience or by the expectation that seemingly esoteric research techniques of today will prove clinically valuable in the future. Hematologic Malignancies: Methods and Techniques is primarily a compilation of methods presented in sufficient detail-by authors with extensive expertise in their field-to serve not only as a reference for seasoned research and clinical laboratory pers- nel, but also as a guide for the less experienced. Moreover, the contributing authors also discuss the pathophysiologic bases and the diagnostic usefulness that underscore each method's clinical relevance. Thus, this volume should be also valuable to clinicians-especially hematologists, oncologists, and path- ogists-often bewildered by an ever increasing flow of new scientific inf- mation, the practical application of which is often either not clearly disclosed or difficult to discern.

Primary care diagnostics involves interpreting and applying information obtained directly from the patient. It re-emphasises the importance of the patient's input into the diagnostic process and of using investigations only after careful consideration has been given to the costs and benefits from both the patient's and the health service's perspectives. The first edition of this book, Patient-Centred Diagnosis, was acclaimed for returning the patient to the centre of diagnostic input and focusing on the interaction between doctor and patient. This revised and augmented new edition, Primary Care Diagnostics, expands on that success; it reflects the broader and equally important issues considered by clinicians or commissioners focusing on the diagnostic approach within primary care, and includes entirely new chapters on cancer and commissioning. Family doctors and other healthcare professionals in the primary care team will find this an enlightening guide. Private and commercial providers of primary care clinical assessment and diagnostic services will also find it of great interest, as will healthcare managers, as well as policy makers and shapers. From reviews of the First Edition: 'Even the more technical sections are expressed in a straightforward language. The style is clear, flowing and engaging. Useful.' THE BRITISH JOURNAL OF GENERAL PRACTICE 'Thought provoking. Clear, understandable and uses real examples from general practice. This is a book for GPs who wish to take a step back from daily practice and think about the reasons behind their actions.' PULSE

Diagnosing Autism Spectrum Disorders: A Lifespan Perspective is the first book of its kind to provide authoritative information for medical and mental health professionals on how to properly evaluate and diagnose autism spectrum disorders in individuals of all ages. * The first book of its kind which demonstrates how to conduct an appropriate diagnostic interview to assess a child for an Autism Spectrum Disorder * Considers the issues of Autism Spectrum Disorders in children, teenagers, and adults * Aimed at both medical and mental health professionals * Includes an in-depth treatment of the entire diagnostic process

This issue of Critical Care Nursing Clinics, guest edited by Stephen Krau, PhD, RN, on the topic of Diagnostic Testing will include the following article subjects: Overview of Diagnostic Testing; Electrodiagnostic Testing; Endoscopic Studies; Bronchoscopy Studies; Radiographic Studies; Angiography; Nuclear Scanning Studies; Intravenous Pyleogram;? Manometric Studies; Ultrasound Studies; Urine Tests; Stool Tests; and Centesis Studies.

This is the second edition of a successful textbook which puts emphasis on the study and knowledge of normality in physical diagnosis, in order to better appreciate, describe, diagnose and treat the abnormal. Clearly written and generously illustrated, it describes in practical step-by-step, head to-toe procedures, the diagnostic examination of the newborn, child and adolescent. It provides a novel approach to the recognition and description of physical findings. The book is divided into 4 sections - Introduction to the Concept of Normality, Taking the History, Physical Examinations, and Specialised Examinations. It emphasises the importance of both subjective and objective observations, of distinguishing between the two, and of the use of exact measurements whenever possible. The book is comprehensive and easy to use, the text flows easily and understandably, unusual or key words are defined, rare diseases, unusual syndromes and name-dropping are avoided wherever possible. In short, this new edition of Pediatric Physical Diagnosis is an enjoyable, invaluable reference for both the beginner and the experienced physician and for trainees specialising in pediatrics and neonatology.

This expert volume in the Diagnostic Pathology series is an excellent point-of-care resource for practitioners at all levels of experience and training. Covering all areas of thoracic pathology, it incorporates the most recent clinical, pathological, and molecular knowledge in this challenging field to provide a comprehensive overview of all key issues relevant to today's practice. Richly illustrated and easy to use, Diagnostic Pathology: Thoracic, 3rd Edition, is a one-stop reference for accurate, complete pathology reports, ideal as a day-to-day reference or as a reliable training resource. Provides a clear framework for a better understanding of the clinical and histopathologic appearances of pulmonary, pleural, and mediastinal entities Features an updated chapter on severe acute respiratory syndrome (SARS) pneumonia with current knowledge, statistics, and pathophysiology on COVID-19, including new images that encompass the radiology, gross pathology, and histopathology of the disease Includes updated criteria, terminology, and classifications for a wide range of tumors from the 2021 World Health Organization Classification of Tumors of the Lung, Pleura, Thymus and Heart, reflecting changes to currently used terminologies for adenocarcinoma, squamous cell carcinoma, neuroendocrine tumors, mesothelioma, soft tissue tumors, benign tumors, and more Covers the recent explosion of molecular genetic information, including new Molecular Pathology sections with new information on the genetic underpinnings of diseases Specifies genetic mutation types associated with each tumor and covers numerous types of molecular tests, including next-generation sequencing studies and how the specific genetic alterations are used to differentiate disease and aid in diagnosis Features over 3,900 high-quality images, including histologic images, gross pathology images, radiologic images, and full-color illustrations as well as more than 750 additional online-only images to help practicing and in-training pathologists reach a confident diagnosis Contains multiple recent consensus clinical practice guidelines for thoracic disease diagnosis, including those for molecular genetic testing and predictive biomarker testing from the College of American Pathologists, the International Association for the Study of Lung Cancer, and the Association for Molecular Pathology Employs consistently templated chapters, bulleted content, key facts, and annotated images for quick, expert reference at the point of care Includes the enhanced eBook version, which allows you to search all text, figures, and references on a variety of devices

Informed by more than thirty years of experience, this guide provides a wealth of insight into the clinical observation, diagnosis and treatment of elderly patients. Organized geographically from (literally) head to toes, it concentrates on the basic geriatric physical examination with each chapter providing general information as well as helpful approaches to specific regional complaints. Emphasis is placed on assisting the dedicated clinician in becoming a more perceptive observer of elderly patients with honed skills in geriatric bedside diagnostic evaluation. The text also includes historical vignettes and anecdotes of great physical diagnosticians to demonstrate the evolution of clinical skills. Tables and line drawings complement the text.

Pocket Guide to Gene Level Diagnostics in Clinical Practice is an abbreviated, pocket-size, quick-reference guide that provides a point-by-point synopsis of the vast wealth of information contained in CRC Handbook of Gene Level Diagnostics in Clinical Practice. All sections and subsections in the Pocket Guide are cross-referenced to corresponding pages in the Handbook. The book works well on its own as a quick reference, but also can be used in conjunction with the larger Handbook for detailed coverage and references to specific information. Pocket Guide to Gene Level Diagnostics in Clinical Practice also includes extensive supplements featuring material not included in the Handbook. These are intended to provide an up-dated, practical source of information useful to anyone involved in molecular diagnostic research and/or service. Supplements are cross-referenced to the main text of the Pocket Guide, that complement and enhance the material covered. Pocket Guide to Gene Level Diagnostics in Clinical Practice will be a handy reference for professionals and students in pathology, biotechnology, biology, and medicine.

100 Diagnostic Challenges in Clinical Medicine is composed of one hundred well-illustrated clinical scenarios and their appropriate investigations. A wide variety of specialties are covered including cardiology, neurology, dermatology, endocrinology, tropical medicine, haematology, metabolic medicine, radiology, ophthalmology, venereology, and infectious diseases. Presenting the relevant investigations corresponding to each case in an interesting and easy-to-read Q&A format concerning diagnosis and management, this book serves as an ideal, and hopefully enjoyable, study aid for medical students and junior doctors who are preparing for clinical examinations in medicine. By solving the problems proposed by these challenging clinical cases, the reader will gain additional practice in diagnosis and treatment strategies.

100 Diagnostic Challenges in Clinical Medicine is composed of one hundred well-illustrated clinical scenarios and their appropriate investigations. A wide variety of specialties are covered including cardiology, neurology, dermatology, endocrinology, tropical medicine, haematology, metabolic medicine, radiology, ophthalmology, venereology, and infectious diseases. Presenting the relevant investigations corresponding to each case in an interesting and easy-to-read Q&A format concerning diagnosis and management, this book serves as an ideal, and hopefully enjoyable, study aid for medical students and junior doctors who are preparing for clinical examinations in medicine. By solving the problems proposed by these challenging clinical cases, the reader will gain additional practice in diagnosis and treatment strategies.

The sixth edition of Ellestad's classic text on cardiac stress testing has been extensively updated and re-written to communicate contemporary understanding of the classical principles of stress testing to clinicians and researchers, students and seasoned practitioners alike. The current techniques for performing stress tests presented herein reflect major technologic advances in imaging, physiologic monitoring and the assessment of cardiovascular risk, addressing fundamental paradigm shifts in interventional, surgical and medical treatment of heart disease. Moreover, the text addresses the dramatic changes that are occurring in patient demographics and the environmental, socioeconomic, gender and genomic factors that crucially impact heart disease and warrant attention when performing cardiac stress testing. Chapters on the physiology of exercise testing including practical details regarding protocols for conducting the stress test, proper supervision, important parameters to be monitored, and the diagnostic and prognostic information to be gleaned from the electrocardiogram set the stage for expanded chapters on the use of cardiac imaging in conjunction with stress testing. Physiologic and metabolic considerations during stress testing are covered in detail. Application of stress testing to special populations, such as women, children, athletes, and individuals in both high and low risk groups are covered in new chapters. Finally, the authors address the use of stress testing in limited resource environments and discuss global changes in the incidence of atherosclerosis, and suggest how stress testing may evolve.

This book provides a complete overview of all modalities used for hand and wrist imaging, along with a complete overview of the various disease entities that can be diagnosed. As a state-of-the-art overview of hand and wrist imaging it is a reference work for radiologists, hand surgeons, orthopedists, traumatologists, rheumatologists and internists and their residents in training. The chapters are written by experts in musculoskeletal radiology from various European countries and the USA.

This volume contains refereed papers submitted by participants of the third week of a six week workshop on Statistics in the Health Sciences held by the Institute of Mathematics and its Applications in Minneapolis, Minnesota during July of 1997. This week was devoted to the closely related topics of Diagnosis and Prediction. Theoretical and applied statisticians from universities, medical and public health schools, government and private research institutions, and pharmaceutical companies involved in prediction problems in the life and social sciences and in diagnostic and screening tests were brought together to discuss and exchange new results and information on these important issues. A number of papers with applications were presented and especially lively discussions ensued involving the critical issues and difficulties in using and interpreting diagnostic tests and implementing mass screening programs. Both frequentist and Bayesian approaches were employed. The importance of predicting or controlling future events such as survival, comparative survival and survival post intervention for a disease or even for certain biological or natural events is growing rapidly. This area of concern was also represented by participants who presented work that devised predictive methodology for a variety of problems mainly from a Bayesian perspective.

Bile acids are increasingly being seen as extremely important carcinogenic agents in cancers of the bile duct, liver, colon, rectum, and oesophagus. They are essential agents involved in lipid digestion and absorption in mammals, however, they also play wide-ranging roles in a variety of disease states ranging from diabetes to cancer. They have evolved exquisite mechanisms for controlling their own synthesis and to ensure that they are produced at correct concentrations and also kept in the correct anatomical environment. It is only when these fine levels of controls are breached that Bile acids become associated with disease. This breaching of control mechanisms can occur through dietary means (e.g. in obesity) whereby excessive levels of Bile acids are produced and converted (via the bacterial flora) to damaging secondary Bile acids. Furthermore, lack of re-absorption of Bile acids can lead to liver pathologies. The atypical movement of Bile acids into the oesophagus, stimulated by episodes of reflux, is linked to oesophageal cancer. In recent years there have been tremendous advances in the understanding of the mechanisms behind the toxicity and bioactivity of different Bile acids and these are covered at length in this book. Prior to the publication of this book there was no single source of information on the toxicology and bioactivity properties of Bile acids. The book also uniquely collects all the relevant information together regarding the role of Bile acids in human disease and the mechanisms underlying Bile acid induced pathology. Additionally, as Bile acids are synthesised from cholesterol, there is wide recognition of the role for Bile acids in obesity linked diseases and this is also covered in this new publication. The book is edited by two experts in the field who have been involved in Bile acid research for several years and who are closely involved with major research groups in the UK, Europe and the US actively engaged in Bile acid research. The Editors have brought together world experts in their own fields to discuss the contribution of Bile acids to various disease pathologies, as well as discussing the mechanisms behind their activity. The book details the plethora of biological activities of this fascinating group of naturally occurring chemicals and provides a one-stop reference for scientists wishing to gain a fuller understanding of Bile acid activity and function.

This book offers an introduction to the newest, fastest-growing field in laboratory science. Explaining and clarifying the molecular techniques used in diagnostic testing, this text provides both entry-level and advanced information. It covers the principles of molecular biology along with genomes and nucleic acid alterations, techniques and instrumentation, and applications of molecular diagnostics. Written by leading experts, this book includes illustrations, tables, and a colorful design to make information easy to find and easy to use. A full-color, 4-page insert shows realistic images of the output for many molecular tests. Learning Objectives open each chapter with an overview of what you should achieve. Key Words are listed and defined at the beginning of each chapter, and are bolded in the text. Review Questions at the end of every chapter let you measure your comprehension. Advanced Concepts are included, but set apart from the rest of the text, for students who want a higher level of learning. Ethics boxes address ethical issues, allowing you to apply your knowledge to real-life scenarios. A glossary of all key words may be easily accessed in the back of the book.

Neurology is primarily characterized by a variety of diseases which seem very similar and are therefore difficult to distinguish between. Skill at differential diagnosis is therefore absolutely paramount.

"Neurological Differential Diagnosis" is a streamlined handbook of "prioritized" differential diagnosis, to be used both in clinical practice and for exam review.

By presenting differential diagnosis in order of frequency and importance, this book provides a practical handbook for clinicians in training, as well as a potential resource for quick board review. Whilst the book covers the most important syndromes and disease entities, readers are referred to other texts for more exhaustive differentials. By limiting differentials in this way - to the most likely and most serious diagnoses - the reader can more easily recall relevant disease processes when faced with a particular clinical situation, whether it be a patient in the emergency room or a difficult question on the board examination.

The book specifically targets neurology residents and fellows, with overlap to neurosurgery and psychiatry. Internal medicine physicians with an interest in neurological problems and medical students looking for an edge in clinical neuroscience would also benefit from this text.

The content is primarily mid-level material, in a pedagogic format.

In order to organize the students' thought processes concise tables and line drawing templates are included. The book is organized into broad chapters by type of disorder and some overlap occurs between particular chapters.

This invaluable guide for nurses improves the skills and knowledge required to consult effectively with patients. It is highly practical, easy to read and comprehend, and is designed for use in daily practice, and as an aid for professional development. The 'Consultation Assessment and Improvement Instrument for Nurses' (CAIIN) concept is introduced, and sample forms, suggested strategies and tables containing key information are also provided. The recent substantial changes that have taken place in health service structures, staffing arrangements and nursing practice have led to more nurses working in first contact roles and within nurse-led services. Nurses at all levels in primary and secondary care, including those in pre-registration training will find this guide vital, as will nurses considering taking on autonomous roles such as independent prescribing. It is also highly suitable for nurse lecturers, nurse managers, learning and health service managers, and undergraduate and postgraduate nursing students.

What's your worst nightmare as a doctor? Missing a life-threatening condition must be among the biggest fears for health professionals. But sometimes the clue to the diagnosis lies in just a single symptom.

"Alarm Bells in Medicine" brings you up to speed on recognising the symptoms of serious illnesses. Internationally renowned authors list the most crucial presenting symptoms in their own specialties that should ring the alarm bells for you. They provide clear information on diagnosis and action. Organised under the relevant specialties, the information is quick and easy to find.

Since patients disclose alarm symptoms in all clinical settings, this book is as relevant for consultants as it is for GPs, as useful for junior doctors as it is for medical students.

Special Features of this book:
Answers the express need of doctors to avoid making diagnostic errors
Each chapter is written by a leading expert in the field
Concise and easy to use

In today's medical education curriculum, it is necessary for students to learn the proper techniques for taking medical histories, performing physical exams, and the appropriate way to educate and inform patients. The best way for a student to learn these skills is through hands-on training with a Standardized Patient (SP) - an actor who has been hired to portray a specific set of health problems and symptoms. This type of training allows students to grasp concepts more quickly and enables faculty physicians to directly observe student's progress and asses their need for further instruction. Working with SP's has become so important in medical education that it is now a component of the USMLE clinical skills assessment exam. As with any tool that is used for teaching, assessment, and certification, it is easier to provide the best service when there are general guidelines for preparers to follow. The coaches who prepare SP's are no exception and there needs to be a guide for those who train SP's for their roles in new doctor education. This handbook is intended as that guide and as a support for those who are involved in training Standardized Patients, from the art of coaching through preparing SP's for the physical exam, to encourage each coach to develop a system that will deliver the best results and, in the end, help train the most competent doctors.

Introducing a new edition of the popular text for medical students, residents, and practitioners on interpreting electrocardiograms in children. Pediatric cardiologists Dr. Myung Park and Dr. Warren Guntheroth teach the vectorial approach to pediatric ECG interpretation in a simple and practical way. How to Read Pediatric ECGs contains over 200 actual size ECG tracings, review questions, case studies for board review. Now with a 2 color design Case Studies teach a systematic approach to interpreting ECG results Review questions at end of each chapter assist with board preparation and self-assessment Actual size tracings allows readers to measure intervals and durations of sample tracings accurately

Evidence based medicine is defined as the conscientious explicit and judicious use of current best evidence in making decisions about the care of individual patients. This superb collection takes a critical view of this concept and examines the economic implications of its imposition.

From "Abdominal Pain" and "Bradycardia" to "Uveitis" and "White Cell Counts," this new pocket guide will provide rapid facts for use in everyday clinical practice.

"Rapid Differential Diagnosis" is the second title in the new Rapid series and is an ideal companion volume to "Rapid Medicine." This handy new book covers the causes for over 350 signs, symptoms, and differentials for radiological, endocrine, haematological, and routine laboratory findings and ECG changes. There is also a thematic index where conditions are sorted by speciality to aid quick look-up.

"Rapid Differential Diagnosis" is authored by Amir H. Sam, a final year medical student from the Royal Free and University College Medical School, London. Dr Huw Beynon, a Consultant General Physician and Rheumatologist at the Royal Free, is the Editorial Advisor for the book. He has been a chief examiner for the MBBS and MRCP for many years.