The development of mechanistic organic chemistry is filled with claims of short-lived reactive intermediates connecting starting material to product. In many ways this book represents a personal odyssey of the editor in this area of chemistry. I well remember my introduction to organic chemistry as an undergraduate working in the laboratories of Shelton Bank at SUNY Albany in the early 1970s, and the excitement and frustration attending the piecing together of the details of a reaction mechanism by working backwards from the stable products of the reaction. In those days the reaction and the reactive intermediates flew by too rapidly to permit direct observation. Thus it came as something of a revelation to me as a graduate student at Yale that it was possible to slow down a reaction and actually "see" such ephemeral species as carbenes and biradicals by spectroscopic methods, by generating them photochemically at cryogenic temperatures. In this monograph several chap ters are devoted to low-temperature studies. Dougherty has described the matrix EPR spectra of biradicals, which were pure conjecture only ten years ago. Michl and Arnold have described the matrix spectroscopy of cyciobutadiene, a molecule that has fascinated organic chemists for over a hundred years. They have shown that by using a combination of matrix spec troscopic methods it is possible to learn nearly as much about the structure of cyciobutadiene, the prototypical antiaromatic biradicaloid, as about that of a common shelf-stable reagent."

Recognized experts present incisive analysis of both fundamental and applied problems in this continuation of a highly acclaimed series. Topics discussed include: A thorough and mathematical treatment of periodic phenomena, with consideration of new theories about the transition between `order' and `chaos'; Impedance spectroscopy as applied to the study of kinetics and mechanisms of electrode processes; The use of stoichiometric numbers in mechanism analysis; The electro-osmotic dewatering of clays with important implications for the processing of industrial waste and geotechnical; stabilization; Magnetic effects in electrolytic processes and the electrolytic Hall effect; and The computer analysis and modeling of mass transfer and fluid flow. These authoritative studies will be invaluable for researchers in engineering, electrochemistry, analytical chemistry, materials science, physical chemistry, and corrosion science.

This book presents a unified outlook on counter-current, ion size exclusion, supercritical fluids, high-performance thin layers, and gas and size exclusion chromatographic techniques used for the separation and purification of organic and inorganic analytes. It also describes a number of green techniques, green sample preparation methods and optimization of solvent consumption in the chromatographic analysis of organic and inorganic analytes. This book offers a valuable resource not only for learners, but also for more experienced chromatographers, conveying a deeper understanding of green chromatographic techniques, green solvents and preparation methods.
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The aim of this volume is to review the state-of-the-art in analytical voltammetry with regard to theory and instrumentation, and show how these relate to the analysis of inorganic, organometallic, organic and biological molecules. Modern voltammetric techniques have practical applications in biological, pharmaceutical and environmental chemistry. The growing importance of voltammetry in the development of modified electrodes and biological electrodes and chemical and biological sensors is also highlighted.

"Microwave-Assisted Sample Preparation for Trace Element Analysis" describes the principles, equipment, and applications involved in sample preparation with microwaves for trace element analysis. The book covers well-established applications as well as new trends in this field. Hot topics such as sample preparation for speciation, metabolomics, and halogen determination, as well as the alternatives of sample preparation for special samples (for example, carbon nanotubes, polymers, petroleum products), are also discussed.

The use of microwaves in sample preparation has increased in recent decades. Several applications of microwaves for sample preparation can be found in the literature for practically all types of sample matrices, especially for the determination of trace elements by atomic spectrometric techniques, safely and cleanly reducing the time involved in this step. Microwave-assisted sample preparation is not only a tool for research but also for routine analysis laboratories; the state-of-the-art in sample preparation in trace element analysis. This book is the only resource for chemists specifically focused on this topic.
The first book to describe the principles, equipment, and applications in microwave-assisted sample preparationWritten by experts in the field who provide a comprehensive overview of the important conceptsIntroduces new alternatives and trends in microwave-assisted techniques

Several state-of-the-art applications of molten salts are presented, such as metal-molten salt systems, room temperature glass formation, and room temperature melts. Several recent examples of applications highlight the importance of molten salts in various industries (batteries, pyrochemical reprocessing of nuclear fuel, synthesis and catalysis). The basic concepts of the structure, dynamics, electrochemistry, interfacial and thermodynamic properties are detailed and relevant experimental methods described. Such fundamental concepts are essential for an in-depth understanding of the physicochemical properties of molten salts in general, including metal-molten salts, glass forming and low temperature melts. Experimental methods for investigating structural, dynamical, electrochemical thermodynamical and interfacial properties are detailed, as also are techniques for data collection and analysis.

Scientists, engineers and technologists will find the volume a valuable reference source covering a wide spectrum of fundamental concepts and modern technologies.

Environmental chemistry is a fast developing science aimed at deciphering fundamental mechanisms ruling the behaviour of pollutants in ecosystems. Applying this knowledge to current environmental issues leads to the remediation of environmental media, and to new, low energy, low emission, sustainable processes. Chapters review analysis and remediation of pollutants such as greenhouse gases, chiral pharmaceuticals, dyes, chlorinated organics, arsenic, toxic metals and pathogen in air, water, plant and soil. Several highlights include the overlooked impact of air pollutants from buildings for health risk, innovative remediation techniques such as bioreactors for gas treatment, electrochemical cleaning of pharmaceuticals, sequestration on Fe-Mn nodules, phytoremediation and photocatalytical inactivation of microbial pathogens.

This book will be a valuable source of information for engineers and students developing novel applied techniques to monitor and clean pollutants in air, wastewater, soils and sediments.

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The functionalization of surfaces on the nanoscale is one of the most fascinating and at the same time challenging topics in science. It is the key to tailoring catalysts, sensors, or devices for solar energy conversion, whose functional principle is based on the interaction of an active solid surface with another (liquid or gaseous) phase. As an example, planar transition metal complexes adsorbed on solid supports are promising candidates for novel heterogeneous catalysts. An important feature of these catalysts, compared to supported metal clusters, is the fact that the active sites, i. e. , the coordinated metal centers with their vacant axial coordination sites, are well de?ned and uniform. Metalloporphyrinoids are particularly suitable in this respect because they combine a structure forming element-the rigid molecular frame, which often induces long range order-with an active site, the coordinated metal ion. Its planar coordination environment leaves two axial coordination sites available for additional ligands. If adsorbed on a surface, one of these axial sites is occupied by the underlying substrate. The resulting electronic interaction with the surface can be used to tailor the electronic structure and thereby the reactivity of the metal center. The remaining site is free for the attachment of molecules (sensor functionality) and/or operates as a reaction center (single-site catalysis). Prototype examples are omnipresent in nature, where in particular metallo-tetrapyrrols play a decisive role in important biological processes, with the most prominent examples being iron porphyrins in heme, magnesium porphyrins in chlorophyll, and cobalt corrin in vitamin B12.

Handbook of Analytical Quality by Design addresses the steps involved in analytical method development and validation in an effort to avoid quality crises in later stages. The AQbD approach significantly enhances method performance and robustness which are crucial during inter-laboratory studies and also affect the analytical lifecycle of the developed method. Sections cover sample preparation problems and the usefulness of the QbD concept involving Quality Risk Management (QRM), Design of Experiments (DoE) and Multivariate (MVT) Statistical Approaches to solve by optimizing the developed method, along with validation for different techniques like HPLC, UPLC, UFLC, LC-MS and electrophoresis. This will be an ideal resource for graduate students and professionals working in the pharmaceutical industry, analytical chemistry, regulatory agencies, and those in related academic fields.

This reference work for analytical chemists has been designed to stimulate fundamental research in pure and applied analytical chemistry. Coverage includes aspects of classical and modern analytical chemistry and the scientific and instrumental fundamentals of analytical methods.

Since several excellent books have appeared on the issue of chemical and biochemical sensing, this compendium concentrates on recent creative new approaches using chemical means for the detection and quanti?cation of - portant analytes. These are presented either from a technical viewpoint or fromtheperspectiveofselectivemolecularrecognitionwitharti?cialreceptor molecules. Consequently, the entire book is subdivided into two categories, i. e. , natural targetsand detection techniques. In the ?rst part, the challenging task of sensing peptides and proteins as wellassaccharidesisaddressedfromseveralperspectives:Smalllibrarieswith maximum diversity are ef?ciently used to reach high af?nity and selectivity of arti?cial hosts for short peptide sequences. Preorganized aromatic vessels are tailored for certain protein epitopes and shown to selectively address the prion protein, and a new conjugation technique operating at physiological conditions on helix-loop-helix motifs, leads to protein binders of exquisite af?nity. Finally, elegant sensor systems for multifunctional carbohydrates in their naturalform,whichuse theprinciple ofphoto-induced electrontransfer for ?uorescence detection, are presented. The second part of this book is devoted to creative detection techniques employing chemical processes: Liposomes with integral self-assembled - acetylene lipid areas show an intense blue color, which gradually changes to red if biological analytes of various sizes speci?cally interact with emb- ded hosts. Principal component analysis and arti?cial neuronal networks are novel methods to quantitatively analyze complex mixtures. An alternative - proach uses arti?cial peptidic pores, which are able to release self-quenched ?uorophoresthataresubstitutedbytighterbindinganalytes.

* Much progress has been made in the last 8 years in understanding the theory and practice of silane coupling agents. A major advance in this direction was the measurement of true equilibrium constants for the hydroly sis and formation of siloxane bonds. Equilibrium constants for bond reten tion are so favorable that a silane coupling agent on silica has a thousandfold advantage for bond retention in the presence of water over an alkoxysilane bond formed from hydroxy-functional polymers and silica. In practice, the bonds of certain epoxies to silane-primed glass resist debonding by water about a thousand times as long as the epoxy bond to unprimed glass. Oxane bonds of silane coupling agents to metal oxides seem to follow the same mechanism of equilibrium hydrolysis and rebonding, although equilibrium constants have not been measured for individual metal-oxygen silicon bonds. This suggests, however, that methods of improving bond retention to glass will also improve the water resistance of bonds to metals. of standard coupling agents with a hydrophobic silane or one Modification with extra siloxane cross-linking have improved the water resistance of bonds to glass and metals another hundredfold over that obtained with single coupling agents."

Over the past years, the chem(o)informatics field has further evolved and new application areas have opened up, for example, in the broadly defined area of chemical biology. In Chemoinformatics and Computational Chemical Biology, leading investigators bring together a detailed series of reviews and methods including, among others, system-directed approaches using small molecules, the design of target-focused compound libraries, the study of molecular selectivity, and the systematic analysis of target-ligand interactions. Furthermore, the book delves into similarity methods, machine learning, probabilistic approaches, fragment-based methods, as well as topics that go beyond the current chemoinformatics spectrum, such as knowledge-based modeling of G protein-coupled receptor structures and computational design of siRNA libraries. As a volume in the highly successful Methods in Molecular Biology (TM) series, this collection provides detailed descriptions and implementation advice that are exceedingly relevant for basic researchers and practitioners in this highly interdisciplinary research and development area. Cutting-edge and unambiguous, Chemoinformatics and Computational Chemical Biology serves as an ideal guide for experts and newcomers alike to this vital and dynamic field of study.

The author has drawn together almost all published methods since 1975 on the determination of anions in all types of matrices. He presents the methods in a logical manner so that the reader can quickly gain access to the method and types of instrumentation available.

A Guide to Materials Characterization and Chemical Analysis Second Edition Edited by John P. Sibilia This book provides an overview of the most current techniques used for chemical analysis, materials evaluation, and materials testing. Over 100 materials methodologies, evaluations, chemical analyses, physical testing, and scientific computing techniques are covered, including the fields of molecular spectroscopy, mass spectroscopy, chromatography, chemical analysis, x-ray analysis, microscopy, surface science, thermal analysis, and polymer characterization. All of the techniques are explained in a clear, easy-to-read format and are discussed in terms of their use, sample requirements, and the underlying chemical, physical, and engineering principles. Many real-life industrial and academic applications are included to give the reader a true feel for the significance and uses of each technique, enabling him or her to identify the best approach for solving a particular problem. For each technique, a section is included that describes its advantages and limitations, along with general references for further reading. A Guide to Materials Characterization and Chemical Analysis, Second Edition, will be of interest to analytical, inorganic, organic, and physical chemists; physicists; materials scientists; chemical engineers; and instructors and students in materials science and instrumental analysis.

The series Molecular Methods of Plant Analysis launches the former 'Modern Methods' into the 'molecular' era with this volume on "Taste and Aroma". Analysis of the plant components interacting with these two senses, so important for the very survival of human beings and, in more recent times, the key to their enjoyment of life as well, is presented with examples of the use of molecular approaches. These include DNA microarrays, antisense technology and RNA gel blot analysis.Some recent advances in plant analysis technology embrace amongst others the use of electroantennography in the detection of physiologically important flower volatiles. An introductory chapter explains what we know about the molecular biology of human taste and aroma receptors, as this has implications for the analysis of plant components interacting with these receptors. As the first volume in the molecular series, this book lays the foundation for others to come.

Although a large number of books are available in the fields of solvent extraction and ion exchangers (extraction by chelation or solvation), this book is the first of its kind in the field of liquid ion exchangers (extraction by ion pair formation) which is an upcoming field in solvent extraction chemistry. There are a number of monographs dealing with various aspects of solvent extraction. However, the present title deals in depth with analytical chemistry liquid ion exchangers. This monograph will be very useful to analytical chemists, environmental scientists, chemical processing and material scientists, research workers in solvent extraction as well as postgraduate students majoring in analytical chemistry.This monograph comprises of several chapters. The introduction deals with elementary ideas about liquid ion exchangers and their comparisons with the solid ion exchange resins. The chapter on solvent extraction covers the general classification of mechanisms of extraction and consideration of the extraction equilibria. There are two chapters pertaining to important characteristics and principles of extraction by both liquid anion as well as liquid cation exchangers. The rest of the chapters deal with the separation of elements with liquid ion exchangers, reverse phase partition chromatography and usage of solvent extraction in separation of metals for industrial purposes.

Field flow fractionation (FFF) is an emerging separation technique, which has been proven successful in the analysis of pharmaceuticals, biotechnology products, polymers, soils, and foods, among others. In this book, Martin Schimpf joins forces with Karin Caldwell and J. Calvin Giddings, two of the primary developers of this technique, to bring you the first comprehensive, one-stop reference on the technique.

This volume has been designed to offer a balanced account of the laboratory synthesis, industrial manufacture and biosynthesis of lipids. Authors describe the synthesis of all the major lipid classes, including new and revised procedures, and there are chapters devoted to the synthesis and manufacture of vitamin E, other natural antioxidants, sugar esters and ethers, and food surfactants. This authoritative work of reference has something for all lipid scientists and technologists. It is directed at chemists and technologists working in oils and fats processing, the food industry, the oleochemicals industry and the pharmaceutical industry; at analytical chemists and quality assurance personnel; and at lipid chemists in academic research laboratories.

Chemical Analysis for Forensic Evidence provides readers with the fundamental framework of forensic analytical chemistry, describing the entire process, from crime scene investigation to evidence sampling, laboratory analysis, quality aspects, and reporting and testifying in court. In doing so, important principles and aspects are demonstrated through the various forensic expertise areas in which analytical chemistry plays a key role, including illicit drugs, explosives, toxicology, fire debris analysis and microtraces such as gunshot residues, glass and fibers. This book illuminates the underlying practical framework that governs how analytical chemistry is used in practice by forensic experts to solve crime. Arian van Asten utilizes a hands-on approach with numerous questions, examples, exercises and illustrations to help solidify key concepts and teach them in an engaging way.

Over the last decade, high performance Capillary electrophoresis (HPCE) has emerged as a powerful and versatile separation technique that promises to rival high performance liquid chromatography when applied to the separation of both charged and neutral species. The high speed and high separation efficiency which can be attained using any of the various modes of HPCE has resulted in the increased use of the technique in a range of analytical environments. The procedures are, however, still in the early stages of development and several barriers remain to their adoption as the technique of choice for a range of analytical problems. One such barrier is the selection and optimization of the conditions required to achieve reproducible separations of analytes and it is in this area that this new book seeks to give assistance. The book is written by an international team of authors, drawn from both academic and industrial users, and the manufacturers of instruments. At its heart are a number of tables, divided into specific application areas. These give details of published separations of a wide range of archetypal analytes, the successful separation conditions and the matrix in which they were presented. These tables are based on separations reported since 1992 and are fully referenced to the original literature. The tables are supported by discussions of the problems that a particular area presents and the strategies and solutions adopted to overcome them. The general areas covered are biochemistry, pharmaceutical science, bioscience, ion analysis, food analysis and environmental science.

Chemiluminescence immunoassay is now established as one of the best alternatives to conventional radioimmunoassay for the quantitation of low concentrations of analytes in complex samples. During the last two decades the technology has evolved into analytical procedures whose performance far exceeds that of immunoassays based on the use of radioactive labels. Without the constraints of radioactivity, the scope of this type of analytical procedure has widened beyond the confines of the specialist clinical chemistry laboratory to other disciplines such as microbiology, veterinary medicine, agriculture, food and environmental testing. This is the first work to present the topic as a subject in its own right.

In order to provide a complete picture of the subject, overviews are presented of the individual areas of chemiluminescence and immunoassay with particular emphasis on the requirements for interfacing chemiluminescent and immunochemical reactions. The possible ways of configuring chemiluminescence immunoassays are described. State-of-the-art chemiluminescence immunoassay systems are covered in detail together with those systems which are commercially available.

The book is aimed at researchers and routine laboratory staff in the life sciences who wish to make use of this high-performance analytical technique and also at those interested in industrial applications of the technology in the food, agricultural and environmental sciences.

Ultrasound is an energy source that has the potential for enhancing many stages of experimental analysis, but analytical chemists generally have limited knowledge of this technique. Analytical Applications of Ultrasound lays the foundations for practicing analytical chemists to consider ways of exploiting ultrasound energy in their research. This timely and unique book covers a broad range of information about ultrasound, providing advances in ultrasound equipment and demonstrations of how this energy has been used to enhance various steps of analysis. Given the limited literature on analytical applications of ultrasound, the authors provide information from other sources that suggest ways in which we can use it in the analytical laboratory. The authors discuss the principles of ultrasound and the variables we must consider in adapting ultrasound to different problems.
* Presents an up-to-date, balanced description of the potential of Ultrasound within Analytical Chemistry
* Discusses ultrasound-based detection techniques in a systematic manner
* Provides an overview of potential applications of ultrasound in a variety of different fields

Integrating both theoretical and applied aspects of electrochemistry, this acclaimed monograph series presents a review of the latest advances in the field. The current volume covers ion and electron transfer across monolayers of organic surfactants, determination of current distributions governed by Laplace's equation, and three other subjects.