Bacteria and fungi are able to aggregate together or on surfaces in densely packed microcolonies, facilitated by extracellular polymeric substances for cell protection and stability. These biofilms have proven to be extremely hard to eradicate and remove once established. In chronic infections, this condition can result in a high degree of morbidity and mortality as regular antibiotic treatments are ineffective against biofilms. In industrial facilities, the formation of biofilms can ruin production and result in enormous financial losses. In this book, the current state of antibiofilm research is presented by experts from around the world. Novel, cutting-edge techniques and new optimized strategies based on established methods are discussed in chapters focused on biofilm prevention, treatment and control for the application in clinical, industrial and veterinary settings. Antibiofilm strategies, such as chemical and enzymatic treatments, surface modification and coatings, quorum sensing inhibition and dispersal induction, phage therapy, cold plasma treatment, hyperbaric oxygen treatment, and metal-based nanomedicine are covered, among many others. This book contributes to the UN's Sustainable Development Goal 3: Good Health and Well-Being and is a valuable resource for healthcare professionals, microbiologists, academics and for educators to inform curricula of universities and colleges.

This volume explores the latest techniques used to study environmental microbial evolution, with a focus on methods capable of addressing deep evolution at long timescales. The chapters in this book are organized into three parts. Part One introduces molecular dating approaches and time calibration ideas that allow for the determination of evolutionary timescales of microbial lineages. Part Two describes several advanced phylogenomic tools such as models for genome tree construction, a taxon sampling method, outgroup-independent tree-rooting methods, and gene family evolution models. Part Three covers techniques used to study trait evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Environmental Microbial Evolution: Methods and Protocols is a valuable tool for all researchers who are interested in learning more about this important and evolving field.

This text contains proceedings of the Federation of European Microbiological Societies Symposium, held at Copenhagen, Denmark, during 4-8 August 1985.

This book illustrates the importance of microbiome interactions in sustainable agriculture and the environment. The chapters of the book provide information pertaining to the vast diversity of microbiomes in many ecosystems and their functional dynamics. The book also discusses bioremediation, space microbiomes, geo microbiomes, coral microbiomes, antibiotic resistomes, and rhizomicrobiome. It also sheds light on the complex syntrophic and other symbiotic interactions between bacteria, protists, plants, and certain animals in agricultural and environmental systems. The book, in turn, provides an understanding of the adaptation, resilience, and evolution of microbial ecosystems. Further, the chapters cover metagenomics analysis of microbiomes of a novel or extreme environments, microbial resilience or temporal fluctuations, symbiosis and co-evolution of the microbiome, and novel microbial interactions in agriculture and environment. Finally, the book elucidates a comprehensive yet representative description of complex structural and functional diversity within the plant and environmental microbiomes to reveal their immense potential. This book covers United Nations Sustainable Developmental Goal 2 towards Zero Hunger.

This thesis studies the impact of food processing on the stability and antioxidant capacity of anthocyanins in aqueous and real food systems. It investigates the effects of temperature and pH on the stability and antioxidant capacity of anthocyanins in aqueous systems and in real semi-solid and solid food systems including bread and biscuits. The results of this thesis offer food manufacturers valuable guidelines on the production of functional foods containing anthocyanins, helping to reduce anthocyanins loss and achieve a desired amount of anthocyanins in foods with extra health benefits.

This edited volume covers all aspects of microbes in consortia; their roles in the ecological balance of soil by mineralize soil nutrients, plant growth promotion, protecting plants from disease by acting as biocontrol agents etc. Step-by-step descriptions are provided to the development and designing strategies of microbial consortia of rhizobacteria, phytohormone producing with biocontrol; ACC-deaminase producing with siderophore producing; vice-versa, and many combinations of multifaceted bacteria. The development of microbial consortia into successful bioinoculant and biofertilizers is also included in various chapters. In addition, molecular mechanisms to study the synergistic behaviors of rhizobacteria, accompanied by numerous helpful schematic drawings. Using phylogeny to justify the molecular similarity among two different bacteria identifies the possibility of microbial synergism, fruitful to development of microbial consortium and establish them in the rhizosphere with consorted mechanisms. In addition, clear drawings are included in support of understanding the natural phenomenon of synergism in below-ground ecosystem. Essential information is provided on ecological management by consorted mechanisms of rhizobacteria that directly affect ‘agriculture sustainability’ and an individual chapter is devoted to the understanding of future research, and addressing bottlenecks and successful steps.  This book assists the academicians, researchers and NGOs in negotiating the steep learning curve involved in gaining the skills needed to perform design and development of microbial consortiums, preparation of PGPR-based fertilizers, which offers significant advantages in terms of pertaining novel knowledge on the groundbreaking research, still ongoing. 

Dysfunction of nuclear-cytoplasmic transport systems has been associated with many human diseases. Thus, understanding of how functional this transport system maintains, or through dysfunction fails to maintain remains the core question in cell biology. In eukaryotic cells, the nuclear envelope (NE) separates the genetic transcription in the nucleus from the translational machinery in the cytoplasm. Thousands of nuclear pore complexes (NPCs) embedded on the NE selectively mediate the bidirectional trafficking of macromolecules such as RNAs and proteins between these two cellular compartments. In this book, the authors integrate recent progress on the structure of NPC and the mechanism of nuclear-cytoplasmic transport system in vitro and in vivo.

The third book in the Sustainable Well Series, Microbiology of Well Biofouling, is the second edition of Practical Manual of Groundwater Microbiology. It is concerned with solving production problems in all types of wells.

See what's new in the new edition:
· Addresses deleterious events in all types of wells in greater detail
· Discusses the generation of mass which interferes with the physical functioning of a well
· Covers the major innovations in the field
· Includes more field applicable material
· Completely revised and updated

The book is a useful reference guide for water well operators, hazardous waste site operators, consulting engineers, public health inspectors, microbiologists, and analytical chemists. It is written with a direct, straight forward approach based upon the advances in the technology over the last twenty years.

With this guide, you understand the phenomena of biofouling, corrosivity, biodegradation, and shifts in hydraulic transmissivity that can be linked to microbial events. Practical approaches to the evaluation of these effects are introduced, including standard and novel methodologies.

Introduction; I. Kahane. Biology of Mollicutes; J.G. Tully. Molecular Biology of Spiroplasmas; J.M. Bove. Mycoplasmas in the Human Urogenital Tract; D. Taylor-Robinson. Mycoplasma Infections of Man; H. Brunner. Mycoplasmas as Immunomodulators; Y. Naot. Mycoplasmas-Animal Plasmas; R.F. Ross. Detection of Adherence of Ureaplasma urealyticum to Bovine Mucosa Fallopian Tube Cells in Culture; A.B. Saada, et al. Serological Identification of Mollicutes; J.G. Tully. Rapid Detection of PhloemRestricted Mollicutes; M. Garnier, et al. Detection and Identification of Mycoplasmas with Diagnostic DNA Probes Complementary to Ribosomal RNA; K.E. Johansson. Mycoplasmas in Cell Culture; M.F. Barile, S. Rottem. Development of a CaptureELISA for the Specific Detection of Mycoplasma pneumoniae in Patients' Material; B. Gerstenecker, E. Jacobs. Identification of Mollicutes by Immunoblotting; S.A. Horowitz. 3 additional articles. Index.

depth overview of the retrovirus family. I have greatly enjoyed and learned from this experience. Each chapter is an excellent introduction to the topic covered and provides a good foundation for further work in the field. Jay A. Levy University of California School of Medicine San Francisco, California REFERENCES Brown, E. W., Yuhki, N., Packer, C., and O'Brien, S. J., 1994, A lion lentivirus related to feline immunodeficiency virus: Epidemiologic and phylogenetic aspects, ,. Viral. 68:5953-5968. Merza, M., Larsson, E., Steen, M., and Morein, B., 1994, Association of a retrovirus with a wasting condition in the Swedish moose, Virology 202:956-961. Contents Chapter 1 The Human Immunodeficiency Viruses Edward Barker, Susan W Barnett, Leonidas Stamatatos, and Jay A. Levy I. Introduction .................................................... 1 TI. Description of Agent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 A. Virus Structure .............................................. 2 B. Genetic Organization and Gene Function ...................... 2 TIL Transmission.................................................... 7 A. General Observations ........................................ 7 B. HIV Transmission by Blood and Blood Products ................ 8 C. HIV Transmission by Genital Fluids ........................... 10 D. HIV Transmission by Other Body Fluids ....................... 12 E. Mother-to-Child Transmission ................................ 12 IV. HIV Infection of the Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 . . . . . . . . . . . . . A. Introduction ................................................. 13 B. HIV-Cell Interaction............ ............... .............. 15 C. CD4-Induced gp120 Conformational Changes .................. 16 D. Soluble CD4-Induced gp120-gp41 Dissociation..... ............ 16 E. gp120 Proteolytic Cleavage ................................... 17 F. pH-Independent Membrane Fusion ............................ 19 G. Transmission of HIV by Cell-to-Cell Fusion.................... 19 H. Additional Cell Surface Receptors for HIV ..................... 20 I. The Envelope Region and Cell Tropism ........ . . . . . . . . . . . . 21 . . . .

This edited book discusses the latest advancements in the area of biofuel development. It covers extensive information regarding different aspects and types of biofuels. The book provides a road map of the various kinds of biofuels available for consideration. It focuses on microbial based power generation, applications of nanotechnology in biofuel development, advancements in molecular techniques, economic and life cycle assessments. The book also highlights the commercialization prospects and economics of the various processes and an overview of the life cycle assessment of the various different kinds of biofuels. The contributors are experienced professors, academicians and scientists associated with renowned laboratories and institutes in India and abroad. This book is of interest to teachers, researchers, biofuel scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students. National and international scientists, policy makers will also find this to be a useful read.

This book describes emerging and established industrial processes of biomining technologies used for the recovery of metals of economic interest from, e.g. mineral ores, mining and electronic wastes using microbiological technologies. Multiple chapters focus on engineering design and operation of biomining systems. Several industrial case studies from China, Chile, Peru, Russia/Kazakhstan and Finland are included, which emphasises the practical approach of the book. The reader not only learns more about the biology, diversity and ecology of microorganisms involved in biomining processes, but also about microbial biomolecular and cultivation tools used in the biomining industry. Special emphasis is put on emerging biotechnologies enabling the use of biomining for recycling metals from e-wastes, waste streams and process waters. Finally, the future impacts and direction of biomining towards sustainability in a metal-demanding world are also highlighted. The book is aimed at an interdisciplinary audience involving operators and researchers working across disciplines including geology, chemical engineering, microbiology and molecular biology. This is reflected by the content of this book, as well as by its authors, who are all leading practitioners and authorities in their fields.

This book critically evaluates recently investigated feedstock for biofuels production. Biofuel sector is rapidly evolving to cater the renewable energy demands. Novel and advanced feedstock are being investigated for their techno-economic feasibility. Environmental concerns, food vs fuel debate, energy security, economic feasibility, and availability are the major drivers for exploring different feedstock for biofuel production. This book explores a wide range of potential biofuels feedstock, their functional concepts, recent advancement, novel technique and critical evaluation with other available biofuel feedstock. This book also discusses future prospects of biofuel production. It is a useful read for students, researchers, faculty, industry and policy makers in the biofuel field.

Big Data technologies have the potential to revolutionize the agriculture sector, in particular food safety and quality practices. This book is designed to provide a foundational understanding of various applications of Big Data in Food Safety. Big Data requires the use of sophisticated approaches for cleaning, processing and extracting useful information to improve decision-making. The contributed volume reviews some of these approaches and algorithms in the context of real-world food safety studies. Food safety and quality related data are being generated in large volumes and from a variety of sources such as farms, processors, retailers, government organizations, and other industries. The editors have included examples of how big data can be used in the fields of bacteriology, virology and mycology to improve food safety. Additional chapters detail how the big data sources are aggregated and used in food safety and quality areas such as food spoilage and quality deterioration along the supply chain, food supply chain traceability, as well as policy and regulations. The volume also contains solutions to address standardization, data interoperability, and other data governance and data related technical challenges. Furthermore, this volume discusses how the application of machine-learning has successfully improved the speed and/or accuracy of many processes in the food supply chain, and also discusses some of the inherent challenges. Included in this volume as well is a practical example of the digital transformation that happened in Dubai, with a particular emphasis on how data is enabling better decision-making in food safety. To complete this volume, researchers discuss how although big data is and will continue to be a major disruptor in the area of food safety, it also raises some important questions with regards to issues such as security/privacy, data control and data governance, all of which must be carefully considered by governments and law makers.

This volume presents a critical analysis and timely synthesis of the past decade of intensive research, development, and demonstrations on the in situ bioremediation of perchlorate in groundwater. The intended audiences include the decision makers, practicing engineers and hydrogeologists who will select, design, and operate these remedial systems, as well as researchers seeking to improve the current state-of-the-art. Our hope is that this volume will serve as a useful resource to assist remediation professionals in applying and developing the technology as effectively as possible. An overview of the current state-of-understanding of perchlorate remediation is followed by a discussion of basic principles of microbial and abiotic processes, and of the engineering and implementation issues underlying the technologies described. Characterization of both anthropogenic and natural sources of perchlorate, including isotopic analysis to distinguish between differing sources, precedes discussions of the advantages, performance, and relative costs of applying a range of remedial technologies. Active, semi-passive, and passive in situ bioremediation are fully described and compared with emphasis on field application. Cost information for each technology, using case studies and analyses of several template sites, covers capital costs, as well as costs for laboratory testing, pilot-scale demonstration, design, system operation, monitoring and maintenance during operations, and demolition and restoration after remediation. In addition, analogous cost data are presented for pump-and-treat systems for each template site to illustrate the potential cost savings associated with the use of alternative approaches. Emerging technologies such as monitored natural attenuation, phytoremediation, and vadose zone bioremediation are described, and field demonstrations are used to illustrate the current stage of maturity and the potential applicability of these approaches for specific situations. Each chapter in this volume has been thoroughly reviewed for technical content by one or more experts in each subject area covered.

This book covers all the aspects of food-grade enzymes, including their classification, kinetics, microbial production, biosynthetic pathways, commodity-wise industrial applications, and downstream processing strategies. The broad focus of this book is on the application of various classes of enzymes in dairy, fruits and vegetables, cereals and oilseeds, meat and poultry, and brewing and food packaging industries. Certain recent areas such as nanotechnological perspective in enzyme immobilization, infusion strategies as well as its efficient usage in food packaging and preservation are some of the salient highlights of this book. This book also discusses the aspects related to application of enzymes in functional food development and shelf life extension of various commodities food products. This book is beneficial for researchers, students, entrepreneurs, and industry experts in broad disciplines such as food processing, food biotechnology, food microbiology, biochemistry, agriculture, biotechnology, biochemical engineering, and bioprocess technology.

This book provides an updated knowledge on the biohydrogen production from industrial and municipal organic waste materials. Microbes are increasingly being included in the hydrogen based biofuel production and this book covers the processes and protocols for biohydrogen production. There is an urgent need of alternative energy research to fulfill the global energy demand. Biohydrogen is a promising source of sustainable and clean energy as it harnessed by biological means. Biohydrogen may be produced by utilizing different waste materials as a substrate, and by optimization of various parameters of bioreactors such as temperature, pH, partial pressure etc. The waste materials used in hydrogen production are categorized as agricultural waste, municipal waste, industrial waste, and other hazardous wastes. Biohydrogen production from wastes materials opened a new opportunity for the widespread use of everlasting renewable energy source. This book is useful for professional scientists, academicians, biotechnologist and environmentalist along with research scholars in various biotechnology and bioenergy industries by addressing the latest research going on in the field of renewal bioenergy production from waste and their global impact on the environment.

This book provides a comprehensive and up-to-date collection of review articles focusing on RNA-mediated regulation in prokaryotes. The various modes of action include the direct interaction with proteins, direct sensing of metabolites or of physical parameters, and the interaction with RNAs to stimulate or prevent binding of ribosomes or to stimulate degradation. Written by leading experts in the field, the book covers small RNA functions, RNA thermometers, riboswitches, the diversity of small RNA-guided CRISPR-Cas defense systems and selected RNA chaperons in both prokaryotic domains, bacteria and archaea. Recent advances towards the computational identification of regulatory RNAs and their targets are included and particular attention is paid to small RNA in pathogenic bacteria. This volume is the only one exclusively covering regulatory RNAs in the prokaryotic domains to date, making it essential literature for anyone interested in RNA function and gene regulation and a valuable resource for teaching these concepts.

Research on the interactions of plants and phytopathogenic fungi has become one of the most interesting and rapidly moving fields in the plant sciences, the findings of which have contributed tremendously to the development of new strategies of plant protection. This book offers insight into the state of present knowledge. Special emphasis is placed on recognition phenomena between plants and fungi, parasitization strategies employed by the phytopathogenic fungi, the action of phytotoxins, the compatibility of pathogens with host plants and the basic resistance of non-host plants as well as cultivar-specific resistance of host plants. Special attention is paid to the gene-for-gene hypothesis for the determination of race-specific resistance, its molecular models and to the nature of race non-specific resistance as well as the population dynamics of plants and the evolution of their basic resistance.

Diatoms are the most species rich group of algae, and they contribute about 20% of annual global carbon fixation. They play major roles in ocean food webs and global biogeochemical cycles. They are also a target of the biotechnology industry because of their nano-patterned silica cell wall and high lipid content. Diatoms have received increasing attention as more genomes became available and because of the development of genome editing tools such as the CRISPR/Cas9 technology, which has made diatoms as genetically tractable as well-established biological model species. This book provides an overview on diatom molecular biology. It brings together international leading experts in the field to discuss the latest data and developments from genes to ecosystems. As the understanding of diatoms is currently experiencing a step change, it is critical to allow for synergistic approaches on diverse aspects of diatom biology and evolution. The books offers fundamental insights into the molecular life of diatoms; at the same time new scientific concepts are developed based on the application of the latest molecular tools and genomic information to explore the fascinating lifestyle of diatoms.

The advent of X-ray Computed Tomography (CT) as a tool for the soil sciences almost 40 years ago has revolutionised the field. Soil is the fragile, thin layer of material that exists above earth's geological substrates upon which so much of life on earth depends. However a major limitation to our understanding of how soils behave and function is due to its complex, opaque structure that hinders our ability to assess its porous architecture without disturbance. X-ray imagery has facilitated the ability to truly observe soil as it exists in three dimensions and across contrasting spatial and temporal scales in the field in an undisturbed fashion. This book gives a comprehensive overview of the "state of the art" in a variety of application areas where this type of imaging is used, including soil water physics and hydrology, agronomic management of soils, and soil-plant-microbe interactions. It provides the necessary details for entry level readers in the crucial areas of sample preparation, scanner optimisation and image processing and analysis. Drawing on experts across the globe, from both academia and industry, the book covers the necessary "dos and don'ts", but also offers insights into the future of both technology and science. The wider application of the book is provided by dedicated chapters on how the data from such imagery can be incorporated into models and how the technology can be interfaced with other relevant technical applications. The book ends with a future outlook from the four editors, each of whom has over 20 years of experience in the application of X-ray CT to soil science.

This timely book discusses antimicrobial drug resistance, specifically, the resistance against the beta-lactam class of antibiotics by Gram-Negative bacteria. The book is broadly divided into five sections. The first section describes the underlying mechanisms of antimicrobial resistance in Gram-negative bacteria. It gives an insight into the beta-lactamases, their types, classification, inhibitors, etc. The second section delves deep into the genetic basis of resistance. It talks about transposons, integrons, insertion sequences associated with antibiotic-resistant genes. The next section describes phenotypic and molecular methods to detect beta-lactam resistance. The fourth section talks about the epidemiology and prevalence of beta-lactamases in the environment. The last section of the book describes the various therapeutic options to combat this growing public threat of antimicrobial resistance. It talks about the current reserve drugs, as well as the newer antibiotic agents that are in the pipeline. This book is essential for clinical practitioners, students, and researchers in basic and medical microbiology.

Every spring, the University of Massachusetts - Amherst welcomes all ''Soils Conference" Scientific Advisory Board members with open arms as we begin the planning process responsible for bringing you quality conferences year after year. With this "homecoming" of sorts comes the promise of reaching across the table and interacting with a wide spectrum of stakeholders, each of them bringing their unique perspective in support of a successful Conference in the fall. This year marks the 20 DEGREES DEGREES anniversary of what started as a couple of thoughtful scientists interested in developing partnerships that together could fuel the environmental cleanup dialogue. Since the passage of the Superfund Law, regulators, academia and industry have come to realize that models that depend exclusively on ''command and control" mandates as the operative underpinning limit our collective ability to bring hazardous waste sites to productive re-use. It is with this concern in mind that the Massachusetts Department of Environmental Protection privatized its cleanup program in 1993, spurring the close-out of over 20,000 sites and spills across the Commonwealth to date, in a manner that is both protective of human health and the environment while also flexible and responsive to varied site uses and redevelopment goals. So we gather together again, this year, to hear our collective stories and share success and challenges just as we share stories at a family gathering. Take a read through the stories contained in these proceedings.

The incidence of food poisoning continues to rise and now there is even greater interest in the subject of microbial food poisoning and food safety, than when the first edition of this popular book was published. As in the first edition of his book, Adrian Eley summarizes information on the principal agents that cause food poisoning and emphasizes their mode of action. Also discussed in chapters written by specially selected expert in the area are epidemilogical features of food poisoning, microbiological control of food production, mycoticoxic fungi and food safety legislation. Each chapter has been comprehensively updated to include any changes in for example laboratory practice, legislation, etc. Also included in this edition is a new chapter on food hygiene. Microbial Food Poisoning, Second Edition, appeals to students studying a wide range of courses, including medical microbiology, food science, and technology, nutrition, general microbiology and environmental health. This book will also be of use to professionals in a range of disciplines including food science, medicine, health sciences and environmental and public health.