Bridging the gap between laboratory observations and industrial practices, this work presents detailed information on recombinant micro-organisms and their applications in industry and agriculture. All recombinant microbes, bacteria, yeasts and fungi are covered.

Handbook of Methods in Aquatic Microbial Ecology is the first comprehensive compilation of 85 fundamental methods in modern aquatic microbial ecology. Each method is presented in a detailed, step-by-step format that allows readers to adopt new methods with little difficulty. The methods represent the state of the art, and many have become standard procedures in microbial research and environmental assessment. The book also presents practical advice on how to apply the methods. It will be an indispensable reference for marine and freshwater research laboratories, environmental assessment laboratories, and industrial research labs concerned with microbial measurements in water.

This Volume covers protocols for in-silico approaches to hydrocarbon microbiology, including the selection and use of appropriate statistical tools for experimental design replication, data analysis, and computer-assisted approaches to data storage, management and utilisation. The application of algorithms to analyse the composition and function of microbial communities is presented, as are prediction tools for biodegradation and protein interactions. The basics of a major open-source programming language, Python, are explained. Protocols for calculating reaction kinetics and thermodynamics are presented, and modelling the environmental fate of hydrocarbons during bioremediation is explained. With the exception of molecular biology studies of molecular interactions, the use of statistics is absolutely essential for both experimental design and data analysis in microbiological research, and indeed in the biomedical sciences in general. Moreover, studies of highly varying systems call for the modelling and/or application of theoretical frameworks. Thus, while two protocols in this Volume are specific to hydrocarbon microbiology, the others are generic, and as such will be of use to researchers investigating a broad range of topics in microbiology and the biomedical sciences in general. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Covers developments in food safety and foodborne illness, organizing information to provide easy access to many topics, both general and specific. Comprehensive summaries of important advances in food science, compiled from over 550 sources worldwide, are presented.

This detailed book provides a collection of protocols for numerous experimental approaches perfected by the authors for lactic acid bacteria (LAB) research. Split in to three parts, the volume delves into the identification and metabolism of LABs, the applications of the bacteria for the food industry, as well as healthy functions of LAB. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lactic Acid Bacteria: Methods and Protocols serves as an ideal inspiration for many research efforts in the domains of food science and health science.

This book has arisen from the Second European Meeting on Bacterial Genetics and Ecology (Bageco-2) held at the University of Wales, College of Cardiff which we organised on 11-12 April 1989. The meeting was attended by some 60 participants from eight European countries and was made possible by partial financial support from the Commission of the European Communities (CEC) and Imperial Chem ical Industries (UK) Ltd. The meeting was organised to discuss modern developments in the genetics of bacteria in aquatic and terrestrial habitats. It followed on from, and complemented, the first meeting of this series organised by Jean-Pierre Gratia in Brussels during April 1987 which concentrated more on medical and epidemiological issues. ! The next meeting will be organised by Michel J. Gauthier in 1991 at Nice, France. If you have been fired with enthusiasm for ecological bacterial genetics after having read this book, and want to attend the next meeting but did not hear about the one in Cardiff, you should write to Dr Gauthier to be put on the address list. A lot is now known about bacterial genetics at the physiological, biochemical and molecular level, and bacterial ecology has developed rapidly over the last 20 years. However, until very recently, few researchers have crossed the divide and linked these two specialisms.

This book covers all the aspects of food-grade enzymes, including their classification, kinetics, microbial production, biosynthetic pathways, commodity-wise industrial applications, and downstream processing strategies. The broad focus of this book is on the application of various classes of enzymes in dairy, fruits and vegetables, cereals and oilseeds, meat and poultry, and brewing and food packaging industries. Certain recent areas such as nanotechnological perspective in enzyme immobilization, infusion strategies as well as its efficient usage in food packaging and preservation are some of the salient highlights of this book. This book also discusses the aspects related to application of enzymes in functional food development and shelf life extension of various commodities food products. This book is beneficial for researchers, students, entrepreneurs, and industry experts in broad disciplines such as food processing, food biotechnology, food microbiology, biochemistry, agriculture, biotechnology, biochemical engineering, and bioprocess technology.

This volume includes treatments of systematics and related topics for both fungi and fungus-like organisms in four eukaryotic supergroups, as well as specialized chapters on nomenclature, techniques and evolution. These organisms are of great interest to mycologists, plant pathologists and others, including those interested in the animal parasitic Microsporidia. Our knowledge of the systematics and evolution of fungi has made great strides since the first edition of this volume, largely driven by molecular phylogenetic analyses. Consensus among mycologists has led to a stable systematic treatment that has since become widely adopted and is incorporated into this second edition, along with a great deal of new information on evolution and ecology. The systematic chapters cover occurrence, distribution, economic importance, morphology and ultrastructure, development of taxonomic theory, classification, and maintenance and culture. Other chapters deal with nomenclatural changes necessitated by revisions of the International Code of Nomenclature for algae, fungi and plants, including the elimination of separate names for asexual states, as well as methods for preservation of cultures and specimens, character evolution and methods for ultrastructural study, the fungal fossil record, and the impact of whole genomes on fungal studies.

This timely book discusses antimicrobial drug resistance, specifically, the resistance against the beta-lactam class of antibiotics by Gram-Negative bacteria. The book is broadly divided into five sections. The first section describes the underlying mechanisms of antimicrobial resistance in Gram-negative bacteria. It gives an insight into the beta-lactamases, their types, classification, inhibitors, etc. The second section delves deep into the genetic basis of resistance. It talks about transposons, integrons, insertion sequences associated with antibiotic-resistant genes. The next section describes phenotypic and molecular methods to detect beta-lactam resistance. The fourth section talks about the epidemiology and prevalence of beta-lactamases in the environment. The last section of the book describes the various therapeutic options to combat this growing public threat of antimicrobial resistance. It talks about the current reserve drugs, as well as the newer antibiotic agents that are in the pipeline. This book is essential for clinical practitioners, students, and researchers in basic and medical microbiology.

This edited volume covers all aspects of microbes in consortia; their roles in the ecological balance of soil by mineralize soil nutrients, plant growth promotion, protecting plants from disease by acting as biocontrol agents etc. Step-by-step descriptions are provided to the development and designing strategies of microbial consortia of rhizobacteria, phytohormone producing with biocontrol; ACC-deaminase producing with siderophore producing; vice-versa, and many combinations of multifaceted bacteria. The development of microbial consortia into successful bioinoculant and biofertilizers is also included in various chapters. In addition, molecular mechanisms to study the synergistic behaviors of rhizobacteria, accompanied by numerous helpful schematic drawings. Using phylogeny to justify the molecular similarity among two different bacteria identifies the possibility of microbial synergism, fruitful to development of microbial consortium and establish them in the rhizosphere with consorted mechanisms. In addition, clear drawings are included in support of understanding the natural phenomenon of synergism in below-ground ecosystem. Essential information is provided on ecological management by consorted mechanisms of rhizobacteria that directly affect ‘agriculture sustainability’ and an individual chapter is devoted to the understanding of future research, and addressing bottlenecks and successful steps.  This book assists the academicians, researchers and NGOs in negotiating the steep learning curve involved in gaining the skills needed to perform design and development of microbial consortiums, preparation of PGPR-based fertilizers, which offers significant advantages in terms of pertaining novel knowledge on the groundbreaking research, still ongoing. 

This book critically evaluates recently investigated feedstock for biofuels production. Biofuel sector is rapidly evolving to cater the renewable energy demands. Novel and advanced feedstock are being investigated for their techno-economic feasibility. Environmental concerns, food vs fuel debate, energy security, economic feasibility, and availability are the major drivers for exploring different feedstock for biofuel production. This book explores a wide range of potential biofuels feedstock, their functional concepts, recent advancement, novel technique and critical evaluation with other available biofuel feedstock. This book also discusses future prospects of biofuel production. It is a useful read for students, researchers, faculty, industry and policy makers in the biofuel field.

Big Data technologies have the potential to revolutionize the agriculture sector, in particular food safety and quality practices. This book is designed to provide a foundational understanding of various applications of Big Data in Food Safety. Big Data requires the use of sophisticated approaches for cleaning, processing and extracting useful information to improve decision-making. The contributed volume reviews some of these approaches and algorithms in the context of real-world food safety studies. Food safety and quality related data are being generated in large volumes and from a variety of sources such as farms, processors, retailers, government organizations, and other industries. The editors have included examples of how big data can be used in the fields of bacteriology, virology and mycology to improve food safety. Additional chapters detail how the big data sources are aggregated and used in food safety and quality areas such as food spoilage and quality deterioration along the supply chain, food supply chain traceability, as well as policy and regulations. The volume also contains solutions to address standardization, data interoperability, and other data governance and data related technical challenges. Furthermore, this volume discusses how the application of machine-learning has successfully improved the speed and/or accuracy of many processes in the food supply chain, and also discusses some of the inherent challenges. Included in this volume as well is a practical example of the digital transformation that happened in Dubai, with a particular emphasis on how data is enabling better decision-making in food safety. To complete this volume, researchers discuss how although big data is and will continue to be a major disruptor in the area of food safety, it also raises some important questions with regards to issues such as security/privacy, data control and data governance, all of which must be carefully considered by governments and law makers.

Resource Recovery in Industrial Waste Waters provides a holistic approach for discovering and harnessing valuable resources from industrial wastewaters, the cutting-edge technologies required, and a discussion on the new findings. In three volumes, the books stress the importance of contaminated waters' remediation, including surface waters, municipal or industrial wastewaters and treating these waters as a new source of nutrients, minerals and energy. It introduces polluted waters as new and sustainable sources, rather than seeing wastewaters as only a source of hazardous organic and inorganic matters. Sections discuss wastewater treatment and recovery and contribute to generate a sustainable approach of wastewater by providing the main advantages and disadvantages of both wastewater/polluted water treatment and recovery.

Resource Recovery in Drinking Water Treatment concentrates on techniques and methods for water purification. The book develops a new approach—resource recovery—toward drinking water, including the role of methods (adsorption, membrane, ion – exchange, biosorption, coagulation, etc.) and nanocomposites (such as biochar, sludge-based composites, chitosan, polymer, magnetic particles, etc.) in water resource recovery. It provides an in-depth overview on emerging water treatment techniques and the resource recovery of materials during the treatment process. Finally, the book aims to introduce polluted waters as new and sustainable sources rather than seeing wastewaters only a source of hazardous organic and inorganic matters. This book is part of a three-volume set that stresses the importance of contaminated water remediation, including surface waters, municipal or industrial wastewaters, and waters as a new source of nutrients, minerals and energy.

Resource Recovery in Municipal Waste Waters provides various municipal wastewater remediation methods and techniques to recover materials from such wastewaters. Sections cover the basic principles of resource recovery, along with the recovery of methane, phosphorous, electricity and metals. The volume covers comprehensive cutting-edge techniques for resource recovery and municipal wastewater treatment and reports on new findings in these areas. It also introduces polluted waters as new and sustainable sources rather than seeing wastewaters as a source of hazardous organic and inorganic matters. The main advantages and disadvantages of both wastewater/polluted water treatment and recovery are also discussed. This three-volume set stresses the importance of contaminated waters remediation, including surface waters, municipal or industrial wastewaters, treating these waters as a new source of nutrients, minerals and energy.

Soils would not exist without the complex and heterogeneous activities of microorganisms. For the third volume of Soil Biology, an international board of renowned scientists shed light on the significant role of these organisms. The following key topics are covered: Microorganisms in bioerosion, humification, mineralization and soil aggregation; Microbial energetics and microbes in biogeochemical processes such as carbon and nitrogen cycles and phosphorus bio-availability; Interactions in the mycorrhizosphere, e.g. between mycorrhizal fungi and bacteria; Impact of microbes on plant nutrient cycling and the possible effects of transgenic rhizospheres on soil fungi; Functions of microbes in specific soil compartments such as soil surface or toxic metal polluted soils; Regulation of microbial activities in functional domains that are influenced by biotic or abiotic factors; Use of marker genes and isotopes as examples for modern techniques in soil microbiology.

Based on the proceedings of the Federation of European Microbiological Societies Symposium held at the Cranfield Institute of Technology, this book serves to highlight a wide variety of areas of civil engineering where microbiological activity needs to be considered more thoroughly by engineers during the design, construction and operation phases of projects. Examples of where microbial processes are now known to have a significant affect include corrosion, deterioration of concrete and other construction materials, impairment of flow in groundwater abstraction systems in pipes and pumps, pressure relief and drainage systems in embankment dams, clogging of irrigation and drainage systems, alteration of soil and rock properties and the production of harmful gases. For a long time many of these "buried" problems have been thought of, purely in terms of physical and chemical processes. What is often happening, but going unnoticed, is some form of microbial activity, which initiates, enhances or is encouraged by other processes. When it is considered that the average bacteria cell is only a few microns in size, then it is hardly surprising if their activity does often go unnoticed. At

Valuable progress has been made in food packaging over the past two decades, reflecting advancements in process efficiency, improved safety and quality throughout the supply chain, and the need to reduce product loss and environmental impact. A new generation of food packaging systems, including active and intelligent packaging, is emerging, based on technological breakthroughs that offer the possibility of extending shelf-life, reducing food loss, and monitoring changes in the food product. Releasing Systems in Active Food Packaging closely examines such a technological breakthrough, active releasing systems, which add compounds such as antimicrobials, antioxidants, flavors, colorants, and other ingredients to packaged food products. Chapters detail examples of recent innovations in active releasing systems, and the authors systematically address their application to different food groups. Such an in-depth approach makes this a useful reference researchers, health professionals, and food and packaging industry professionals interesting in innovative food packaging technologies.

The advent of X-ray Computed Tomography (CT) as a tool for the soil sciences almost 40 years ago has revolutionised the field. Soil is the fragile, thin layer of material that exists above earth's geological substrates upon which so much of life on earth depends. However a major limitation to our understanding of how soils behave and function is due to its complex, opaque structure that hinders our ability to assess its porous architecture without disturbance. X-ray imagery has facilitated the ability to truly observe soil as it exists in three dimensions and across contrasting spatial and temporal scales in the field in an undisturbed fashion. This book gives a comprehensive overview of the "state of the art" in a variety of application areas where this type of imaging is used, including soil water physics and hydrology, agronomic management of soils, and soil-plant-microbe interactions. It provides the necessary details for entry level readers in the crucial areas of sample preparation, scanner optimisation and image processing and analysis. Drawing on experts across the globe, from both academia and industry, the book covers the necessary "dos and don'ts", but also offers insights into the future of both technology and science. The wider application of the book is provided by dedicated chapters on how the data from such imagery can be incorporated into models and how the technology can be interfaced with other relevant technical applications. The book ends with a future outlook from the four editors, each of whom has over 20 years of experience in the application of X-ray CT to soil science.

This book highlights the recent advances in the field of microbial engineering and its application in human healthcare. It underscores the systemic and synthetic biology approaches for engineering microbes and discusses novel treatments for inflammatory bowel diseases based on engineered probiotics. The book also reviews the different options and methods for engineering microbes, ranging from recombinant DNA technology to designing microbes for targeting specific sites and delivering therapeutics. Further, it discusses genetically engineered microorganisms for smart diagnostics and describes current approaches in microbial gene editing using CRISPR-Cas9-based tools. Lastly, it summarizes the potential applications of human microbiome engineering in improving human health and explores potential strategies for scaling-up the production of engineered microbial strains for commercial purposes, as well as the challenges. Given its scope, this book is a valuable resource for students, researchers, academics and entrepreneurs interested in understanding microbial engineering for the production of commercial products.

This book provides fundamentals, highlights recent developments and offers new perspectives relating to the use of electrolyzed water (EW) as an emerging user- and environmental-friendly broad-spectrum sanitizer, with particular focus on the food industry. It addresses the generation, inactivation, pesticide degradation and safety of food by EW, illustrates the mechanism of the germicidal action of EW and its antimicrobial efficacy against a variety of microorganisms in suspensions. In addition, the sanitizing effects of combining EW with various chemical and physical sanitizing technologies have been evaluated, and recent developments and applications of EW in various areas including fruits and vegetables, meat, aquatic products, environment sterilization, livestock and agriculture has been described. The book can be a go-to reference book of EW for: (1) Researchers who need to understand the role of various parameters in its generation, the bactericidal mechanism of EW and its wide applications for further research and development; (2) Equipment producers who need comprehensive understanding of various factors (e.g. type of electrolyte, flow rates of water and electrolyte) which govern the efficacy of EW and developing its generators; (3) Food processors who need good understanding of EW in order to implement it in the operations and supervisors who need to balance the advantages and limitations of EW and ensuring its safe use.

An up-to-date view of molecular mechanisms for investigating microbial communities and their biological activities, this new volume of Environmental Microbiology: Methods and Protocols looks at recent advances that are having a big impact on the field such as metagenomics and other "omics" technologies, NanoSIMS, as well as stable isotope probing and more. Conveniently divided into four parts, the first section looks at methods involved in sampling environmental microorganisms, the second profiles different methods for investigating the diversity and composition of microbial communities, the third focuses on techniques for analyzing biological activities in situ, and the final section examines high throughput "omics" approaches for the characterization of environmental microbial communities. This book was written as part of the highly successful Methods in Molecular Biology series, and, as such, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Fully updated and authoritative, Environmental Microbiology: Methods and Protocols, Second Edition aims to provide an unprecedented glimpse into the structure, composition, and activity of microbial communities across diverse environments and illuminate their impact on global ecological processes.

Bioactive compounds in food, known for their positive health effects, can be lost during handling after harvest, processing and storage. While most foods are exposed to processing to increase shelf life and edibility and to ensure microbial safety, conventional processing methods may have disadvantages, such as decreasing the nutritional quality of foods, long processing times, high temperature and high energy uses. For these reasons, novel non-thermal food processing technologies (including HPP, ultrasound) and novel thermal food processing technologies (including microwave/Ohmic heating) have become widespread. This book provides a critical evaluation of the effects of conventional, novel non-thermal, and thermal food processing techniques on the retention and bioaccessibility of bioactive compounds in food materials. Within these three categories, many different processing methods are included: fermentation/germination, drying, extrusion, and modified atmosphere packaging, as well as novel technologies, such as microwave heating, ultrasound, high pressure processing, ozonation, and membrane separation processes.