This book provides an integrated review of the human ocular microbiome. It documents the discovery of ocular surface microbes by the conventional cultivable method and next generation sequencing technologies in both healthy and diseased (keratitis, uveitis, endophthalmitis, blepharitis, conjunctivitis etc.) eyes. The book further discusses the confounding factors that influence the microbiome, mycobiome and virome. The chapters cover niche-specificity with reference to skin, eyelid- margin, hands etc. It highlights the concept of core genera, dysbiosis and discriminating genera and covers the functional relevance of the dysbiotic microbiome, mycobiome and virome with respect to ocular diseases. The book includes topics on the relevance of molecular mechanisms, including quorum-sensing and mucin metabolism to ocular disorders, such as dry eye; and, microbiome-based therapies for treating of ocular disorders like vernal keratoconjunctivitis. The book is essential for microbiologists studying the human eye, ophthalmologists treating eye infection and trauma. It also caters to students of medical microbiology and medicine.

This book addresses the diversity of tropical microorganisms and its applications in agriculture, renewable energy production and environmental protection. It covers several tropical habitats such as rain forests, mangroves, sea and river waters and describes how microorganisms isolated from these regions can be used to control insects and plant diseases, to improve sugar cane and biofuels production among other applications. The book also aims to bring researchers' attention to the potential of tropical microorganisms for biotechnological purposes, an area that is still far from being well explored.

This volume discusses both the latest experimental research in bioelectrosynthesis and current applications. Beginning with an introduction into the "electrification of biotechnology" as well as the underlying fundamentals, the volume then discusses a wide range of topics based on the interfacing of biotechnological and electrochemical reaction steps. It includes contributions on the different aspects of bioelectrochemical applications for synthesis purposes, i.e. the production of fine and platform chemicals based on enzymatically or microbially catalyzed reactions driven by electric energy. The volume finishes with a summary and outlook chapter which gives an overview of the current status of the field and future perspectives. Edited by experts in the field, and authored by a wide range of international researchers, this volume assesses how research from today's lab bench can be developed into industrial applications, and is of interest to researchers in academia and industry.

Algae are simple, primitive, heterogeneous, autotrophic, eukaryotic or prokaryotic organisms that lead a symbiotic, parasitic or free-living mode of life. Microalgae and macroalgae possess great potential in various fields of application. Microalgae are ubiquitous and extremely diverse microorganisms that can accumulate toxic contaminants and heavy metals from wastewater, making them a superior candidate to become a powerful nanofactory. Algae were discovered to reduce the presence of metal ions, and afterwards aid in the biosynthesis of nanoparticles. Since algae-mediated biogenic nanoparticles are eco-friendly, cost-effective, high-yielding, speedy and energy-efficient, a large number of studies have been published on them in the last few years. This book focuses on recent progress on the utilization of algae for the synthesis of nanoparticles, their characterization and the possible mechanisms involved. Bioprospecting Algae for Nanosized Materials describes the synthesis of algal nanomaterials and its application in various fields for sustainable development. This book outlines the procedures to prepare phyconanomaterials, techniques to utilize the nanomaterials, and applications in agriculture, environment and medicine.

This open access book offers the first comprehensive account of the pan-genome concept and its manifold implications. The realization that the genetic repertoire of a biological species always encompasses more than the genome of each individual is one of the earliest examples of big data in biology that opened biology to the unbounded. The study of genetic variation observed within a species challenges existing views and has profound consequences for our understanding of the fundamental mechanisms underpinning bacterial biology and evolution. The underlying rationale extends well beyond the initial prokaryotic focus to all kingdoms of life and evolves into similar concepts for metagenomes, phenomes and epigenomes. The book's respective chapters address a range of topics, from the serendipitous emergence of the pan-genome concept and its impacts on the fields of microbiology, vaccinology and antimicrobial resistance, to the study of microbial communities, bioinformatic applications and mathematical models that tie in with complex systems and economic theory. Given its scope, the book will appeal to a broad readership interested in population dynamics, evolutionary biology and genomics.

Bioactive natural compounds have gained attention in recent years due to their potential health benefits, including reducing the risk of diabetes, cancer, and cardiovascular diseases. These benefits derive from bioactive compounds' anti-tumor, anti-inflammatory, anti-oxidative, anti-hypertensive and anti-hyperlipidemic activities, which serve in addition to their basic nutritional functions. Over the last decade, researchers have investigated the health impact of bioactive compounds in detail, and the development of food applications has attracted great interest. Consumer demand has surged for functional foods (nutraceuticals), superfoods, and tailor-made foods, generated by supplementing traditional food products with bioactive ingredients. Food Bioactives and Health offers comprehensive coverage of the properties and health effects of food bioactives in view of new trends in processing, food science and food technology. Starting with the metabolic characteristics of polyphenols, glucosinolates, and other food bioactives, the text then dives into their impact on human health and recent applications in the world of food technology. For food scientists, food technologists, and product developers looking to understand the role of food bioactives in health and develop applications in personalized nutrition, functional foods and nutraceuticals, Food Bioactives and Health serves as a one-stop reference.

Achieving environmental sustainability with rapid industrialization is a major challenge of current scenario worldwide. As globally evident, industries are the key economic drivers, but are also the major polluters as untreated/partially treated effluents discharged from the industries is usually thrown into the aquatic resources and also dumped unattended. Industrial effluents are considered as the major sources of environmental pollution as these contains highly toxic and hazardous pollutants, which reaches far off areas due to the medium of dispersion and thus, create ecological nuisance and health hazards in living beings. Hence, there is an urgent to find ecofriendly solution to deal with industrial waste, and develop sustainable methods for treating/detoxifying wastewater before its release into the environment. Being a low cost and eco-friendly clean technology, bioremediation can be a sustainable alternative to conventional remediation technologies for treatment and management of industrial wastes to protect public health and environment. Therefore, this book (Volume I) covers the bioremediation of different industrial wastes viz. tannery wastewater, pulp and paper mill wastewater, distillery wastewater, acid mine tailing wastes, and many more; which are lacking in a comprehensive manner in previous literature at one place. A separate chapter dedicated to major industries and type of waste produced by them is also included. This book will appeal to students, researchers, scientists, industry persons and professionals in field of microbiology, biotechnology, environmental sciences, eco-toxicology, environmental remediation and waste management and other relevant areas, who aspire to work on the biodegradation and bioremediation of industrial wastes for environmental safety.

This book presents a comprehensive overview of DNA barcoding and molecular phylogeny, along with a number of case studies. It discusses a number of areas where DNA barcoding can be applied, such as clinical microbiology, especially in relation to infection management; DNA database management; and plant -animal interactions, and also presents valuable information on the DNA barcoding and molecular phylogeny of microbes, algae, elasmobranchs, fishes, birds and ruminant mammals. Furthermore it features unique case studies describing DNA barcoding of reptiles dwelling in Saudi Arabian deserts, genetic variation studies in both wild and hatchery populations of Anabas testudineus, DNA barcoding and molecular phylogeny of Ichthyoplankton and juvenile fishes of Kuantan River in Malaysia, and barcoding and molecular phylogenetic analysis of indigenous bacteria from fishes dwelling in a tropical tidal river. Moreover, since prompt identification and management of invasive species is vital to prevent economic and ecological loss, the book includes a chapter on DNA barcoding of invasive species. Given its scope, this book will appeal not only to researchers, teachers and students around the globe, but also to general readers.

Plastic is one of the widely used polymers around the globe since its discovery. It is highly impossible to think the ease of life without the aid of plastic. Every year billion tons of plastic waste gets accumulated in the environment and leads to death of both marine and terrestrial animals. Plastic is very durable and needs around 1000 years to degrade under the natural environment. The present book illustrates the importance and significance of the bioremediation to tackle the problem of plastic waste. Previously, we have reported elite rhizobacterial isolates (Lysinibacillus fusiformis strain VASB14/WL and Bacillus cereus strain VASB1/TS) of Avicennia marina Vierh (Forsk.) from the West Coast of India with the potential to degrade plastic (polythene). The present book attempted to address the bioremediation scenario of plastic waste (including micro plastic) using microbes with bacteria in particular. Various strategies used to tackle with the plastic waste were highlighted with case studies of plastic waste management, including in vitro, in situ and ex situ with a special reference to biodegradation technology. After the biodegradation of the plastic using microbes, the generated plastic (polythene) degradation products (PE-DPs) were also documented using GC-MS technique followed by their deleterious effect on both animal and plant systems. The book also enhances the awareness of the plastic-free society and also suggests some alternative materials to be used instead of plastic. Lastly, the book suggests/recommends the strategies to be followed by the lawmakers in the government organizations/non-government organizations/social organizations to frame the regulations and guidelines to implement at mass level to reduce the generation of plastic waste.

Emulsifiers, also known as surfactants, are often added to processed foods to improve stability, texture, or shelf life. These additives are regulated by national agencies, such as the FDA, or multi-national authorities, such as the EEC or WHO. The amphiphilic molecules function by assisting the dispersion of mutually insoluble phases and stabilizing the resulting colloids, emulsions, and foams. Emulsifiers can interact with other food components such as carbohydrates, proteins, water, and ions to produce complexes and mesophases. These interactions may enhance or disrupt structures and affect functional properties of finished foods. In dairy processing, small molecule emulsifiers may displace dairy proteins from oil/water and air/water interfaces, which affects stability and properties of the foams and emulsions. In baked products, emulsifiers contribute to secondary functionalities, such as dough strengthening and anti-staling. Synthetic food emulsifiers suffer from the stigma of chemical names on a product's ingredient statement. Modern consumers are seeking products that are "all natural." Fortunately, there are a number of natural ingredients that are surface-active, such as lecithin, milk proteins, and some protein-containing hydrocolloids. Mayonnaise, for example, is stabilized by egg yolk. This book can serve as both a guide for professionals in the food industry to provide an understanding of emulsifier functionality, and a stimulus for further innovation. Students of food science will find this to be a valuable resource.

Several cyanobacterial species can produce powerful toxins that provide a serious threat for water quality, other aquatic organisms, and human health. These harmful cyanobacteria are especially prominent in freshwater ecosystems, and are a major concern for water managers.The purpose of this work is to provide an up-to-date overview of the advances in our knowledge of harmful cyanobacteria. The work is directed towards graduate students and scientists in aquatic microbiology, aquatic ecology, environmental toxicology, and water management, and academic professionals in water management and environmental policy.

In Heme, Chlorophyll, and Bilins: Methods and Protocols, an interdisciplinary panel of hands-on investigators describe in detail how to work successfully with chlorophyll, heme, and bilins in biological, medical, chemical, and biochemical research. Each method is presented by a researcher who actually uses it on a daily basis and includes step-by-step instructions and pertinent tricks-of-the-trade that often make the difference between laboratory success and failure. Topics range from methods for the analysis of tetrapyrroles, heme, and hemoproteins, to the biosynthesis and analysis of chlorophyll and bilins

Microbial Nanobionics: Volume 1, State of the Art, discusses a wide range of microbial systems and their utilization in biogenic synthesis of metallic nanoparticles. The rich biodiversity of microbes makes them excellent candidates for potential nanoparticle synthesis biofactories. Through a better understanding of the biochemical and molecular mechanisms of the microbial biosynthesis of metal nanoparticles, the rate of synthesis can be better developed and the monodispersity of the product can be enhanced. The characteristics of nanoparticles can be controlled via optimization of important parameters, such as temperature, pH, concentration and pressure, which regulate microbe growth conditions and cellular and enzymatic activities. Large scale microbial synthesis of nanoparticles is a sustainable method due to the non-hazardous, non-toxic and economical nature of these processes. The applications of microbial synthesis of nanoparticles are wide and varied, spanning the industrial, biomedical and environmental fields. Biomedical applications include improved and more targeted antimicrobials, biosensing, imaging and drug delivery. In the environmental fields, nanoparticles are used for bioremediation of diverse contaminants, water treatment, catalysis and production of clean energy. With the expected growth of microbial nanotechnology, this volume will serve as a comprehensive and timely reference.

This detailed volume explores rice molecular biology, genetic engineering, and genome editing technologies. Dividing into three parts, the book covers subjects such as genetic engineering and tissue culture of rice, including efficient methods for rice transformation and regeneration, genome editing, targeted integration, and gene stacking in rice, including multiple methods utilizing CRISPR systems for targeted gene knock-out or genome modification via base editing, and diverse methods describing bioinformatic, molecular, and cellular analyses in rice. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Rice Genome Engineering and Gene Editing: Methods and Protocols serves as a valuable resource for researchers worldwide striving to further their efforts on advancing research and producing genetically improved rice varieties.

This book presents a comprehensive review of renewable energy-based sustainable drying techniques for developing countries. Aspiring towards a world with zero food waste, the book has provided discussion on sustainable drying techniques in terms of energy efficiency. The socio-economic condition of each developing country is unique; therefore, has specific technological requirements. As such, the book presents discussions on food waste scenario around the world, the socio-economic status of developing countries and their correlation with food. The book gives an overview of the quality aspects of drying, along with the required energy and time to retain these features. Additionally, a method of selecting drying techniques for developing countries, taking the cost and safety factor into consideration, has been discussed extensively Also, the renewable and non-renewable energy resources of low income, lower-middle income, middle income, and high-income developing countries have been analyzed and presented. The book also highlights the available drying techniques that are currently being practiced by the consumers and industries of developing countries. The book recommends ten sustainable drying technologies for the developing countries and describes their working principle. Discussion on potential challenges for sustainable drying technology adoption is also presented. The book presents up-to-date research on sustainable drying techniques and their impact on developing countries to reduce food waste. Food waste is not only a humanitarian concern but also a threat to environmental sustainability. Currently, one-third of all produced food is being wasted, when nearly 805 million people - including children remain undernourished on a daily basis. In an effort to solve this crisis, a number of food preservations techniques are being practiced in food supply chain. Drying is one such preservation technique that prevents microbial proliferation, slows enzymatic reaction and preserves the physio-chemical properties of food. Albeit, drying is an effective means of food preservation; it is also highly energy-intensive. Developing countries do not have sufficient energy and financial resources to adopt conventional (expensive and high energy) drying techniques. As such, this is the first reference work dedicated to discussing the prospects and challenges of sustainable (renewable energy based and inexpensive) drying techniques for developing countries in order to reduce food waste. Sustainable food drying techniques in developing countries: Prospects and Challenges is a singular work in the field of food preservation and affordable drying technology.

Membrane Systems for Hydrogen Production offers an overview of advanced technologies in the field of both catalysts and membrane technologies for hydrogen productions and energy saving. Catalysts play an irreplaceable role in chemical engineering for carrying out reaction at industrial level. Membrane processes are today well-recognized technologies in many fields, such as water and wastewater treatment, gas separation and purification, etc. This book relates these two fields and their role in electrochemical hydrogen production by presenting 5 specific chapters where the catalysts are compared to the membrane technology. The purpose of this book is to provide an overview on recently developed catalysts which work in combination with membrane operations for energy savings. This combination provides an example of strategies for engineering development and process intensification of interest for both industrial and developing countries.

Molecular Aspects of Plant Beneficial Microbes in Agriculture explores their diverse interactions, including the pathogenic and symbiotic relationship which leads to either a decrease or increase in crop productivity. Focusing on these environmentally-friendly approaches, the book explores their potential in changing climatic conditions. It presents the exploration and regulation of beneficial microbes in offering sustainable and alternative solutions to the use of chemicals in agriculture. The beneficial microbes presented here are capable of contributing to nutrient balance, growth regulators, suppressing pathogens, orchestrating immune response and improving crop performance. The book also offers insights into the advancements in DNA technology and bioinformatic approaches which have provided in-depth knowledge about the molecular arsenal involved in mineral uptake, nitrogen fixation, growth promotion and biocontrol attributes.

Fungi are an important link in the food webs of all ecosystems. They have immense potential and comprise a myriad of useful bioactive compounds. Fungi feature in a wide range of diverse processes and applications in modern agriculture, the food science industry, and the pharmaceutical industry. In the food and drink arena, the role of fungi is historically important in the form of mushrooms and in fermented foods as yeasts for baking and brewing. These roles are supplemented by the use of fungal food processing enzymes and additives, and more recently in the development of protein-based foodstuffs from fungi. Additionally, they are used in the formulation of biofertilizers and biopesticides used as biostimulants and bioprotectants of crops. The practical use of newer techniques such as genetic recombination and robotics have revolutionized the modem agricultural biotechnology industry, and have created an enormous range of possible further applications of fungal products. Myco-materials created from mycelia (the root-like parts of fungi) are gaining attention as a sustainable alternative for a wide range of materials. They are being used as insulation, sustainable packaging, foam inserts, and even "eco-leather." In fact, mycelium bricks are pound-for-pound stronger than concrete. In addition, medicinal uses of fungal species have been historically recorded as important agents in the pharmaceutical sciences. The potential for myco-materials seems limitless. The field of mycology and its application has become an increasingly important component in the education of industrial biotechnology. This book on applied mycology provides information helpful for developing entrepreneurial opportunities with fungi. This volume explains both the basic science and the applications of mycology and bio-resource technology with special emphasis on entrepreneurial applications. It offers a complete, one-stop resource for those interested in microbiology, food and agricultural science, medical mycology, and for those in industrial biotechnology.

This volume is a comprehensive review of the structure/function and biology of molecules belonging to the TGF-[beta] superfamily. Because molecules in this family have very diverse biological roles, the editors have chosen to focus on the parts they play in the specific areas of inflammation and wound/fracture healing. Whilst molecules in the TGF-[beta] superfamily have been extensively studied, there are few, if any, publications which have taken a broad perspective on this family, most having chosen to focus on just one very small area. This book is therefore unusual in that it offers a comprehensive overview of the current state of the field, providing both in-depth and essential background material suitable for both clinicians and scientists alike.

Under continual attack from both microbial pathogens and multicellular parasites, insects must cope with immune challenges every day of their lives. However, this has not prevented them from becoming the most successful group of animals on the planet. Insects possess highly-developed innate immune systems which have been fine-tuned by an arms race with pathogens spanning hundreds of millions of years of evolutionary history. Recent discoveries are revealing both an unexpected degree of specificity and an indication of immunological memory - the functional hallmark of vertebrate immunity. The study of insect immune systems has accelerated rapidly in recent years and is now becoming an important interdisciplinary field. Furthermore, insects are a phenomenally rich and diverse source of antimicrobial chemicals. Some of these are already being seriously considered as potential therapeutic agents to control microbes such as MRSA. Despite a burgeoning interest in the field, this is the first book to provide a coherent synthesis and is clearly structured around two broadly themed sections: mechanisms of immunity and evolutionary ecology. This novel text adopts an interdisciplinary and concept-driven approach, integrating insights from immunology, molecular biology, ecology, evolutionary biology, parasitology, and epidemiology. It features contributions from an international team of leading experts. Insect Infection and Immunity is suitable for both graduate students and researchers interested in insect immunity from either an evolutionary, genetical, physiological or molecular perspective. Due to its interdisciplinary and concept-driven approach, it will also appeal to a broader audience of immunologists, parasitologists and evolutionary biologists requiring a concise overview.

Microbial Endophytes: Functional Biology and Applications focuses on endophytic bacteria and fungi, including information on foundational endophytes and the latest advances in relevant genomics, proteomics and nanotechnological aspects. The book provides insights into the molecular aspects of plant endophytes and their interactions and applications, also exploring the potential commercialization of endophytic microorganisms and their use as bio fertilizers, in biocontrol, and as bioactive compounds for other sustainable applications. Coverage of important and emerging legal considerations relevant to those working to implement these important bacteria in production processes is also included.

This textbook explores the complex nature of soil biological communities and their environments, and covers deserts, rainforests, seasonal tropical forests, dry deciduous forests, and island environments in the tropical zone. It provides essential information on soil biology concepts, ecological processes, plant-soil feedback, trophic structure, and land use effects on soil's biological properties. The book also offers an updated approach to soil biota and microbiota and their interactions with plants that regulate the structures and functions of tropical ecosystems. Uniquely, it addresses island environments and natural disasters, shedding new light on soil organisms recovering tropical ecosystem functions. Further topics include ecological processes, plant-soil interactions, trophic communities, molecular approaches, and land use, making the book a valuable asset for students, educators and researchers engaged in the Environmental Sciences, Biodiversity and Conservation, Soil Ecology, Soil Biology, Ecology, Zoology, and Soil Biota Classification using classical and molecular tools.

This book contains a collection of critical reviews on the expression of biologically functional proteins in" Leishmania" and "Trypanosoma," which was written by renowned researchers on this field. Species belonging to these trypanosomatids genera are etiological agents of leishmaniasis, Chagas disease and sleeping sickness that are extremely debilitating human infection diseases, which remain a major health problem especially in countries from Latin America, Africa and Middle East. Substantiating the problem, the currently accepted drugs for these diseases are quiet unsatisfying due to their low efficacy and high toxicity. In order to solve these real problems, several research groups around the world have become involved in the study and identification of novel potential targets in the trypanosomatid cell. Since proteins are key macromolecules involved in crucial metabolic processes of all living cells, studies have focused on the expression of specific proteins produced by "Leishmania "and "Trypanosoma" by means of different biochemical, molecular and proteomic approaches in order to explore them as targets for understanding the parasite life cycle and developing new strategies against trypanosomiasis. With these proposals in mind, the book Proteins and Proteomics of "Leishmania "and "Trypanosoma" encompasses (i) an integrated view about the biochemistry of parasites belonging to the "Leishmania" and "Trypanosoma "genera; (ii) an updated review on the expression of biologically relevant proteins by human pathogenic trypanosomatids and their possible role in the interaction with host cells/molecules as well as a target for development of both alternative chemotherapies and vaccine; and (iii) several pictures, diagrams and tables that can be used to illustrate both undergraduate and postgraduate teaching as well as scientific lectures, being a useful resource for students and researchers."

Advances in Applied Microbiology, Volume 111, continues the comprehensive reach of this widely read and authoritative review source in microbiology. Users will find invaluable references and information on a variety of areas relating to the topic.