1 Einleitung.- 1.1 Rechtliche Hygieneregelung.- 1.2 Aspekte zum Hygienekonzept.- Literatur.- 2 Begriffe zur Lebensmittelhygiene.- 2.1 Lebensmittel.- 2.2 Lebensmittelhygiene.- 2.3 Leichtverderbliche Lebensmittel.- 2.4 Verpackte Lebensmittel.- 2.5 Herstellen.- 2.6 Behandeln.- 2.7 In-Verkehr-bringen.- 2.8 Verzehren.- 2.9 Nachteilige Beeinflussung.- 2.10 Genusstauglichkeit/ Genusswert.- 2.11 Mindesthaltbarkeitsdatum.- 2.12 Bedarfsgegenstande.- Literatur.- 3 Lebensmittelmikrobiologie.- 3.1 Einteilung von Mikroorganismen.- 3.1.1 Bakterien und Bakteriensporen.- 3.1.2 Schimmelpilze.- 3.1.3 Hefepilze.- 3.1.4 Viren.- 3.2 Groessenordnung von Mikroorganismen und Viren.- 3.3 Erkennbarmachung von Keimen.- 3.4 Vermehrungsformen von Mikroorganismen.- 3.4.1 Ungeschlechtliche Fortpflanzung.- 3.4.2 Geschlechtliche Fortpflanzung von Hefen.- 3.5 Gesundheitsgefahrdende Mikroorganismen, Lebensmittelverderber und Nutzlinge.- 3.5.1 Gesundheitsgefahrdende Mikroorganismen und schadliche Stoffwechselprodukte.- 3.5.2 Lebensmittelverderber.- 3.5.3 Technologisch erwunschte Mikroorganismen.- Literatur.- 4 Wachstumsvoraussetzungen fur Mikroorganismen - Beeinflussung der Vermehrung.- 4.1 Wachstumsfaktoren.- 4.1.1 Nahrstoffangebot.- 4.1.2 Wasseraktivitat und Feuchtigkeit.- 4.1.3 pH-Wert.- 4.1.4 Temperatur.- 4.1.5 Redoxpotential.- 4.2 Beeinflussung des Wachstums von Mikroorganismen.- Literatur.- 5 Mikrobielle Gefahrdung von Produkten.- 5.1 Lebensmittelverderb.- 5.1.1 Faulnis.- 5.1.2 Garung.- 5.1.3 Sauerung.- 5.1.4 Ranziditat.- 5.1.5 Schimmeln.- 5.2 Lebensmittelvergiftung.- 5.2.1 Salmonellen.- 5.2.2 Staphylococcus aureus.- 5.2.3 Clostridium perfringens.- 5.2.4 Clostridium botulinum.- 5.2.5 Bacillus cereus.- 5.2.6 Listeria monocytogenes.- 5.3 Gefahrdungen physikalischen Ursprungs.- Literatur.- 6 Schadlingsbefall und weitere Gefahrdungen.- 6.1 Insekten.- 6.2 Nager und Voegel.- 6.3 Schadlingsbekampfung als Teil der Betriebshygiene.- 6.4 Chemische Schadensquellen.- 6.5 Physikalische Schadensquellen.- 7 Raum-und Anlagenhygiene.- 7.1 Reinigung und Desinfektion.- 7.1.1 Reinigungsverfahren.- 7.1.2 Desinfektion.- 7.1.3 Reinigungsintervalle.- 7.2 Raumtemperaturen.- 7.3 Konstruktive Anlagenhygiene.- Literatur.- 8 Stichprobenplane.- 8.1 Mikrobiologische Stichprobenplane.- 8.1.1 2-Klassenplan.- 8.1.2 3-Klassenplan.- 8.2 Stichprobenplane fur die sensorische Prufung.- Literatur.- 9 Betriebseigene Massnahmen und Kontrollen - HACCP-Konzept.- 9.1 Produktbeschreibung, Ermittlung potentieller Gefahren, Identifizierung kritischer Punkte (HACCP-Grundsatze 1-3).- 9.2 Festlegung und Durchfuhrung des Verfahrens zur UEberwachung und Kontrolle der kritischen Punkte (HACCP-Grundsatze 4, 5).- 9.3 UEberprufung der Eigenkontrollsysteme, Dokumentation (HACCP-Grundsatze 6, 7).- Literatur.

The development of biotechnological innovations is quickly becoming a globalized phenomenon as emerging nations are making major strides to compete with more developed economies. Though efforts to bridge the gap between emerging and developed nations have been successful, there are still many barriers that need to be overcome. Comparative Approaches to Biotechnology Development and Use in Developed and Emerging Nations evaluates the importance of manufacturing biotechnological products around the world. Highlighting a comparative analysis of public policies, technological policies, innovations, and marketing capabilities of developed and emerging nations, this publication is a pivotal reference source for government officers, policy makers, academics, and practitioners.

The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. The chapter "Polymeric Nanoparticle-Mediated Gene Delivery for Lung Cancer Treatment" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

Handbook of Microalgae: Biotechnology Advances offers complete coverage of marine microalgae, including biology, production techniques, biotechnological applications, economic perspectives of applications, and environmental effects of marine microalgae blooms. With contributions from world experts, Handbook of Microalgae: Biotechnology Advances focuses on microalgae from an organism perspective to offer a complete picture from evolution to biofuel.

This book explores critical principles and new concepts in bioengineering, integrating the biological, physical and chemical laws and principles that provide a foundation for the field. Both biological and engineering perspectives are included, with key topics such as the physical-chemical properties of cells, tissues and organs; principles of molecules; composition and interplay in physiological scenarios; and the complex physiological functions of heart, neuronal cells, muscle cells and tissues. Chapters evaluate the emerging fields of nanotechnology, drug delivery concepts, biomaterials, and regenerative therapy. The leading individuals and events are introduced along with their critical research. Bioengineering: A Conceptual Approach is a valuable resource for professionals or researchers interested in understanding the central elements of bioengineering. Advanced-level students in biomedical engineering and computer science will also find this book valuable as a secondary textbook or reference.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Despite ongoing progress in nano- and biomaterial sciences, large scale bioprocessing of nanoparticles remains a great challenge, especially because of the difficulties in removing unwanted elements during processing in food, pharmaceutical and feed industry at production level. This book presents magnetic nanoparticles and a novel technology for the upscaling of protein separation. The results come from the EU Project "MagPro2Life", which was conducted in cooperation of several european institutions and companies.

The growing scale of plant-based chemicals for industrial use has generated considerable interest in developing methods to meet their desired production levels. Among various available strategies for their production, the development of Agrobacterium rhizogenes mediated hairy root cultures (HRCs) is generally considered the most feasible approach. Additionally, several proof-of-principle experiments have demonstrated the practical feasibility of HRCs in the plant-based remediation of environment pollutants, biotransformation of important compounds, and production of therapeutic proteins. Given that hairy root biotechnology has now been recognized as a promising and highly dynamic research area, this book offers a timely update on recent advances, and approaches hairy roots as a multifaceted biological tool for various applications. Further, it seeks to investigate the loopholes in existing methodologies, identify remaining challenges and find potential solutions by presenting well thought-out scientific discussions from various eminent research groups working on hairy root biotechnology. This book provides detailed conceptual and practical information on HRC-based research, along with relevant case studies. The content is divided into three broad sections, namely (i) Hairy Roots and Secondary Metabolism, (ii) Progressive Applications, and (iii) Novel Approaches and Future Prospects. By informing the research and teaching community about the major strides made in HRC-based interventions in plant biology and their applications, the book is sure to spark further research in this fascinating field.

This six volume book set examines a range of topics and applications related to biotechnology. Volumes include fermentation and algal biotechnologies; agricultural biotechnology; medical biotechnology, biopharmaceutics; biosafety, bioethics, biotechnology policy; microbiomes; bioenergy and environmental biotechnology for sustainable development. The topics address significant aspects of the dairy and fermented foods; crop genetics, breeding and genomics; microalgae and novel products; molecular diagnostics and DNA forensics; biosafety, bioethics and legal issues in biotechnology; biotechnology policy advocacy, enlightenment and engagement with stakeholders; medical and pharmaceutical biotechnology; omics and bioinformatics; waste recycling, biofuels and environmental remediation; animal genetics, breeding and genomics; genetic resources conservation and utilization; medicinal and underutilized plants; medical insect biotechnology, genomics and molecular genetics of pests; microbiomes and microbial biotechnology; biotechnology education and curriculum development; and water and waste water research. Volumes include: I: Fermentation and Algal Biotechnologies for the Food, Beverage and other Bioproduct Industries II: Agricultural Biotechnology, Biodiversity and Bio-resources Conservation and Utilization III: Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics IV: Biosafety and Bioethics in Biotechnology: Policy, Advocacy, and Capacity Building V: Microbiomes and Emerging Applications VI: Bioenergy and Environmental Biotechnology for Sustainable Development The book is a timely knowledge product that documents key issues on advances in biotechnology for use by a variety of readers including postgraduate students, professionals in the field, policy makers, science advocacy groups.

The population of the world continues to increase at an alarming rate. The trouble linked with overpopulation ranges from food and water scarcity to inadequacy of space for organisms. Overpopulation is also linked with several other demographic hazards, for instance, population blooming will not only result in exhaustion of natural repositories, but it will also induce intense pressure on the world economy. Today nanotechnology is often discussed as a key discipline of research but it has positive and negative aspects. Also, due to industrialization and ever-increasing population, nano-pollution has been an emerging topic among scientists for investigation and debate. Nanotechnology measures any substance on a macromolecular scale, molecular scale, and even atomic scale. More importantly, nanotechnology deals with the manipulation and control of any matter at the dimension of a single nanometer. Nanotechnology and nanoparticles (NPs) play important roles in sustainable development and environmental challenges as well. NPs possess both harmful and beneficial effects on the environment and its harboring components, such as microbes, plants, and humans. There are many beneficial impacts exerted by nanoparticles, however, including their role in the management of waste water and soil treatment, cosmetics, food packaging, agriculture, biomedicines, pharmaceuticals, renewable energies, and environmental remedies. Conversely, NPs also show some toxic effects on microbes, plants, as well as human beings. It has been reported that use of nanotechnological products leads to the more accumulation of NPs in soil and aquatic ecosystems, which may be detrimental for living organisms. Further, toxic effects of NPs on microbes, invertebrates, and aquatic organisms including algae, has been measured. Scientists have also reported on the negative impact of NPs on plants by discussing the delivery of NPs in plants. Additionally, scientists have also showed that NPs interact with plant cells, which results in alterations in growth, biological function, gene expression, and development. Thus, there has been much investigated and reported on NPs and plant interactions in the last decade. This book discusses the most recent work on NPs and plant interaction, which should be useful for scientists working in nanotechnology across a wide variety of disciplines.

Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features * Easy to follow by the users as the experiments have been written in simple language and step-wise manner. * Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. * Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. * Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. * Flow-chart of each experiment will quickly guide the users in performing the experiments.

This book presents a comprehensive study on microextrusion-based 3D bioprinting technologies for bioinks with various crosslinking mechanisms, chiefly focusing on the bioprinting process and bioink properties to provide readers with a better understanding of this state-of-the-art technology. Further, it summarizes a number of general criteria and research routes for microextrusion-based 3D bioprinting using three experimental studies based on shear-thinning, thermo-sensitive and non-viscous hydrogel bioinks. The book also presents sample applications in the areas of stem cells and cell matrix interaction. The book highlights pioneering results in the development of bioprinting technologies and bioinks, which were published in high-quality journals such as Advanced Materials, Biofabrication and ACS Biomaterials Science & Engineering. These include an in-situ crosslinking strategy that overcomes the viscosity limits for bioinks, which is virtually impossible using conventional strategies, and can be generalized for other bioink formulations.

Evidence-Based Validation of Herbal Medicines brings together current thinking and practice in the areas of characterization and validation of natural products. This book reviews all aspects of evaluation and development of medicines from plant sources, including their cultivation, collection, phytochemical and phyto-pharmacological evaluation, and therapeutic potential. Emphasis is placed on describing the full range of evidence-based analytical and bio-analytical techniques used to characterize natural products, including -omic technologies, phyto-chemical analysis, hyphenated techniques, and many more.

This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

The second volume in a series which aims to focus on advances in computational biology. This volume discusses such topics as: statistical analysis of protein sequences; progress in large-scale sequence analysis; and the architecture of loops in proteins.

In the past half century, filamentous fungi have grown in commercial importance not only in the food industry but also as sources of pharmaceutical agents for the treatment of infectious and metabolic diseases and of specialty proteins and enzymes used to process foods, fortify detergents, and perform biotransformations. The commercial impact of molds is also measured on a negative scale since some of these organisms are significant as pathogens of crop plants, agents of food spoilage, and sources of toxic and carcinogenic compounds. Recent advances in the molecular genetics of filamentous fungi are finding increased application in the pharmaceutical, agricultural, and enzyme industries, and this trend promises to continue as the genomics of fungi is explored and new techniques to speed genetic manipulation become available.

This volume focuses on the filamentous fungi and highlights the advances of the past decade, both in methodology and in the understanding of genomic organization and regulation of gene and pathway expression.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.

The entire range of the developmental process in plants is regulated by a shift in the hormonal concentration, tissue sensitivity and their interaction with the factors operating around the plants. Phytohormones play a crucial role in regulating the direction of plant in a coordinated fashion in association with metabolism that provides energy and the building blocks to generate the form that we recognize as a plant. Out of the recognized hormones, attention has largely been focused on Auxins, Gibberellins, Cytokinins, Abscisic acid, Ethylene and more recently on Brassinosteroids. In this book we are providing the information about a brassinosteroids that again confirm its status as phytohormones because it has significant impact on various aspects of the plant life and its ubiquitous distribution throughout the plant kingdom. Brassinosteroids are generating a significant impact on plant growth and development, photosynthesis, transpiration, ion uptake and transport, induces specific changes in leaf anatomy and chloroplast structure. This book is not an encyclopedia of reviews but includes a selected collection of newly written, integrated, illustrated reviews describing our knowledge of brassinosteroids. The aim of this book is to tell all about brassinosteroids, by the present time. The various chapters incorporate both theoretical and practical aspects and may serve as baseline information for future researches through which significant development is possible. It is intended that this book will be useful to the students, teachers and researchers, both in universities and research institutes, especially in relation to biological and agricultural sciences.

This book examines how biotechnology can improve livestock breeding and farming, and thereby also animal products. In the first chapters the reader will discover which techniques and approaches are currently used to improve animal breeding, animal health and the value of animal products. Particular attention is given to reproduction techniques, animal nutrition and livestock vaccines that not only enhance animal health but also have a significant effect on human health by ensuring safe food procurement and preventing zoonotic diseases. In addition, modern biotechnology can increase not only productivity but also the consistency and quality of animal food, fiber and medical products. In the second part of the book, issues such as how animal biotechnology could affect the environment and the important topic of animal waste management are explored. In the concluding chapter, the authors discuss future challenges related to animal biotechnology. This work will appeal to a wide readership, from scientists and professionals working in animal production, to those in farm animal management and veterinary science.

The rapid growth of biotechnology and drug design, based on rational principles of biopolymer interactions, has generated many developments in the field of biophysical chemistry. This series presents overviews of these developments and of other topical areas that are attracting interest in the field, from methodological developments in high-resolution NMR spectroscopy and molecular modelling to advances in structural chemistry and mechanistic studies of proteins and other biological compounds crucial for drug design.