Recent developments in stem cell biology have opened new directions in cell therapy. This book provides the state-of-the-art developments in using biomaterials as artificial niches for engineering stem cells, both for the purpose of better understanding their biology under 3D biomimetic conditions as well as for developing new strategies for efficient long term maintenance and directed differentiation of stem cells into various therapeutic lineages. Animal and human stem cells of both embryonic and adult origin are discussed with applications ranging from nerve regeneration, orthopedics, cardiovascular therapy, blood cell generation and cancer therapy. Both synthetic and natural biomaterials are reviewed with emphasis on how material-stem cell interactions direct specific signaling pathways and ultimately modulate the cell fate. This book is valuable for biomaterial scientists, tissue engineers, clinicians as well as stem cell biologists involved in basic research and applications of adult and embryonic stem cells.

This book explores the ethnobiology of corals by examining the various ways in which humans, past and present, have exploited and taken care of coral and coralline habitats. This book will bring the educated general audience closer to corals by exploring the various circumstances of human-coral coexistence by providing scientifically sound and jargon-free perspectives and experiences from across the globe. Corals are a vital part of the marine environment since they promote and sustain marine and global biodiversity while providing numerous other environmental and cultural services. Countless valuable coral conservation efforts are published in academic and general audience venues on a daily basis. However relevant, few of these reports show a direct, deeper understanding of the intimate relationship between people and corals throughout the world's societies. Ethnobiology of Corals and Coral Reefs establishes an intimate bond between the audience and the wonder of corals and their importance to humankind.

VLSI 2010 Annual Symposium will present extended versions of the best papers presented in ISVLSI 2010 conference. The areas covered by the papers will include among others: Emerging Trends in VLSI, Nanoelectronics, Molecular, Biological and Quantum Computing. MEMS, VLSI Circuits and Systems, Field-programmable and Reconfigurable Systems, System Level Design, System-on-a-Chip Design, Application-Specific Low Power, VLSI System Design, System Issues in Complexity, Low Power, Heat Dissipation, Power Awareness in VLSI Design, Test and Verification, Mixed-Signal Design and Analysis, Electrical/Packaging Co-Design, Physical Design, Intellectual property creating and sharing.

The aim of this manual is to provide a comprehensive guide to the methods involved in collecting, preparing and screening plants for bioactive properties for manipulating key ruminal fermentation pathways and against gastrointestinal pathogens. The manual will better equip the reader with methodological approaches to initiate screening programmes to test for bioactivity in native plants and find natural alternatives to chemicals for manipulating ruminal fermentation and gut health. The manual provides isotopic and non-isotopic techniques to efficiently screen plants or plant parts for a range of potential bioactives for livestock production. Each chapter has been contributed by experts in the field and methods have been presented in a format that is easily reproducible in the laboratory. It is hoped that this manual will be of great value to students, researchers and those involved in developing efficient and environmentally friendly livestock production systems."

This manual offers detailed protocols for fluorescence in situ hybridization (FISH) and comparative genomic hybridization approaches, which have been successfully used to study various aspects of genomic behavior and alterations. Methods using different probe and cell types, tissues and organisms, such as mammalians, fish, amphibians (including lampbrush-chromosomes), insects, plants and microorganisms are described in 57 chapters. In addition to multicolor FISH procedures and special applications such as the characterization of marker chromosomes, breakpoints, cryptic aberrations, nuclear architectures and epigenetic changes, as well as comparative genomic hybridization studies, this 2nd edition describes how FISH can be combined with other techniques. The latter include immunostaining, electron microscopy, single cell electrophoresis and microdissection. This well-received application guide provides essential protocols for beginning FISHers and FISH experts alike working in the fields of human genetics, microbiology, animal and plant sciences.

White moves from a simple proposition maintaining that all individuals seek suitable surroundings to propose a provocative approach to social and political action. Rooting his position in modern life sciences and particularly in sociobiology and neurobiology, he establishes an "IMPish" model that is "interactional," "mentalist," and "populational." Interactional in that both heredity and environment are credited for due influence on individuals' traits; mentalist in that individuals' actions can be purposeful rather than simply determined; and populational in his insistence that the unique persona must not be slighted in the rush to fashion statistics. Applying his behavioral principles most notably relevant to self-selection and using examples derived from modern political action, White examines the importance of these fundamental orientations in the social and political orders. The work has implications for policy assessment and re-formulation. It constitutes a challenge to much of the widely accepted contemporary political theory and public policy approaches.

Adding to a vitally important cycle of publications covering the latest research developments in our understanding of neoplasms affecting the human central nervous system, this edition focuses on numerous aspects of pineal, pituitary, and spinal tumors. As with the previous volumes in the series, this latest work addresses a central imperative in cancer research the need to standardize classifications, written definitions and investigative guidelines in order to achieve a measure of shared objectivity among academics engaged in one of the most important medical endeavors of our era. It brings together the very latest work by oncologists, neurosurgeons, physicians, research scientists, and pathologists, providing the medical community with a wealth of data and results that, taken together, will advance the cause of cancer research. The volume synthesizes work on diagnosis, drug development, and therapeutic approaches that are typically scattered in a variety of journals and books. It features promising recent work in applying molecular genetics to clinical practice and evidence-based therapy, covering molecular profiling of tumors as well as a number of surgical treatments such as resection and radiosurgery. Together with its counterpart publications, it represents a much-needed central resource that will inform and guide future research efforts."

Bringing together techniques and methods currently being applied to the study of exocytosis, "Exocytosis Methods" collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission. The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion. The volume continues with several model systems that are being employed to unravel the complexities of exocytosis. Written for the "Neuromethods" series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results.

Practical and authoritative, "Exocytosis Methods" seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field."

Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the spectacular growth in statistical methodologies for molecular evolution. Evolutionary Genomics: Statistical and Computational Methods is intended to bring together the more recent developments in the statistical methodology and the challenges that followed as a result of rapidly improving sequencing technologies. Presented by top scientists from a variety of disciplines, the collection includes a wide spectrum of articles encompassing theoretical works and hands-on tutorials, as well as many reviews with key biological insight. Volume 2 begins with phylogenomics and continues with in-depth coverage of natural selection, recombination, and genomic innovation. The remaining chapters treat topics of more recent interest, including population genomics, -omics studies, and computational issues related to the handling of large-scale genomic data. Written in the highly successful Methods in Molecular Biology (TM) series format, this work provides the kind of advice on methodology and implementation that is crucial for getting ahead in genomic data analyses. Comprehensive and cutting-edge, Evolutionary Genomics: Statistical and Computational Methods is a treasure chest of state-of the-art methods to study genomic and omics data, certain to inspire both young and experienced readers to join the interdisciplinary field of evolutionary genomics.

This book addresses in detail multifaceted approaches to boosting nutrient use efficiency (NUE) that are modified by plant interactions with environmental variables and combine physiological, microbial, biotechnological and agronomic aspects. Conveying an in-depth understanding of the topic will spark the development of new cultivars and strains to induce NUE, coupled with best management practices that will immensely benefit agricultural systems, safeguarding their soil, water, and air quality. Written by recognized experts in the field, the book is intended to provide students, scientists and policymakers with essential insights into holistic approaches to NUE, as well as an overview of some successful case studies. In the present understanding of agriculture, NUE represents a question of process optimization in response to the increasing fragility of our natural resources base and threats to food grain security across the globe. Further improving nutrient use efficiency is a prerequisite to reducing production costs, expanding crop acreage into non-competitive marginal lands with low nutrient resources, and preventing environmental contamination. The nutrients most commonly limiting plant growth are N, P, K, S and micronutrients like Fe, Zn, B and Mo. NUE depends on the ability to efficiently take up the nutrient from the soil, but also on transport, storage, mobilization, usage within the plant and the environment. A number of approaches can help us to understand NUE as a whole. One involves adopting best crop management practices that take into account root-induced rhizosphere processes, which play a pivotal role in controlling nutrient dynamics in the soil-plant-atmosphere continuum. New technologies, from basic tools like leaf color charts to sophisticated sensor-based systems and laser land leveling, can reduce the dependency on laboratory assistance and manual labor. Another approach concerns the development of crop plants through genetic manipulations that allow them to take up and assimilate nutrients more efficiently, as well as identifying processes of plant responses to nutrient deficiency stress and exploring natural genetic variation. Though only recently introduced, the ability of microbial inoculants to induce NUE is gaining in importance, as the loss, immobilization, release and availability of nutrients are mediated by soil microbial processes.

This book represents different types of progress in hydrogeology, including conceptualization changes, different approaches to simulating groundwater flow and transport new hydrogeophysical methods. Each chapter extends or summarizes a recent development in hydrogeology, with forward-looking statements regarding the challenges and strengths that are faced. While the title and scope is broad, there are several sub-themes that connect the chapters. Themes include theoretical advances in conceptualization and modeling of hydrogeologic problems. Conceptual advances are further tempered by insights arising from observations from both field and laboratory work.

This book integrates discoveries from recent years to show the diversity of molecular mechanisms that contribute to memory consolidation, reconsolidation, extinction, and forgetting. It provides a special focus on the processes that govern functional and structural plasticity of dendritic spines. In nine chapters, new and important ideas related to learning and memory processes will be presented. Themes discussed include the role of AMPA receptors in memory, two signalling cascades involved in local spine remodelling and memory, the role of extracellular matrix proteins in memory, the regulation of gene expression and protein translation, and mechanisms of retrieval-induced memory modulation and forgetting. We believe that the study of these topics represents a great step toward understanding the complexity of the brain and the processes it governs.

For all introductory genetics courses. Concepts of Genetics emphasises the fundamental ideas of genetics, while exploring modern techniques and applications of genetic analysis. This best-selling text continues to provide understandable explanations of complex, analytical topics and recognises the importance of teaching students how to become effective problem solvers. The 12th Edition has been extensively updated to provide comprehensive coverage of important, emerging topics such as CRISPR-Cas and the study of posttranscriptional gene regulation in eukaryotes. An expanded emphasis on ethical considerations that genetics is bringing into everyday life is addressed in Genetics, Ethics, and Society and Case Study features.

The contributed volume puts emphasis on a superior role of water in (bio)systems exposed to a mechanical stimulus. It is well known that water plays an extraordinary role in our life. It feeds mammalian or other organism after distributing over its whole volume to support certain physiological and locomotive (friction-adhesion) processes to mention but two of them, both of extreme relevance. Water content, not only in the mammalian organism but also in other biosystems such as whether those of soil which is equipped with microbiome or the ones pertinent to plants, having their own natural network of water vessels, is always subjected to a force field.The decisive force field applied to the biosystems makes them biomechanically agitated irrespective of whether they are subjected to external or internal force-field conditions. It ought to be noted that the decisive mechanical factor shows up in a close relation with the space-and-time scale in which it is causing certain specific phenomena to occur.The scale problem, emphasizing the range of action of gravitational force, thus the millimeter or bigger force vs. distance scale, is supposed to enter the so-called macroscale approach to water transportation through soil or plants' roots system. It is merely related to a percolation problem, which assumes to properly inspect the random network architecture assigned to the biosystems invoked. The capillarity conditions turn out to be of prior importance, and the porous-medium effect has to be treated, and solved in a fairly approximate way.The deeper the scale is penetrated by a force-exerting and hydrated agent the more non-gravitational force fields manifest. This can be envisaged in terms of the corresponding thermodynamic (non-Newtonian) forces, and the phenomena of interest are mostly attributed to suitable changes of the osmotic pressure. In low Reynolds number conditions, thus in the (sub)micrometer distance-scale zone, they are related with the corresponding viscosity changes of the aqueous, e.g. cytoplasmatic solutions, of semi-diluted and concentrated (but also electrolytic) characteristics. For example, they can be observed in articulating systems of mammals, in their skin, and to some extent, in other living beings, such as lizards, geckos or even insects. Through their articulating devices an external mechanical stimulus is transmitted from macro- to nanoscale, wherein the corresponding osmotic-pressure conditions apply. The content of the proposed work can be distributed twofold. First, the biomechanical mammalian-type (or, similar) systems with extraordinary relevance of water for their functioning will be presented, also including a presentation of water itself as a key physicochemical system/medium. Second, the suitably chosen related systems, mainly of soil and plant addressing provenience, will be examined thoroughly. As a common denominator of all of them, it is proposed to look at their hydrophobic and/or (de)hydration effects, and how do they impact on their basic mechanical (and related, such as chemo-mechanical or piezoelectric, etc.) properties. An additional tacit assumption employed throughout the monograph concerns statistical scalability of the presented biosystems which is equivalent to take for granted a certain similarity between local and global system's properties, mostly those of mechanical nature. The presented work's chapters also focus on biodiversity and ecological aspects in the world of animals and plants, and the related systems. The chapters' contents underscore the bioinspiration as the key landmark of the proposed monograph.

In recent years there has been significant attention paid on the endophytic research by various groups working within this domain. Mutualistic endophytic microbes with an emphasis on the relatively understudied fungal endophytes are the focus of this special book. Plants are associated with micro-organisms: endophytic bacteria and fungi, which live inter- and intra-cellularly without inducing pathogenic symptoms, but have active biochemical and genetic interactions with their host. Endophytes play vital roles as plant growth promoters, biocontrol agents, biosurfactant producers, enzymes and secondary metabolite producers, as well as providing a new hidden repertoire of bioactive natural products with uses in pharmaceutical, agrochemical and other biotechnological applications. The increasing interest in endophytic research generates significant progress in our understanding of the host-endophyte relationship at molecular and genetic level. The bio-prospection of microbial endophytes has led to exciting possibilities for their biotechnological application as biocontrol agent, bioactive metabolites, and other useful traits. Apart from these virtues, the microbial endophytes may be adapted to the complex metabolism of many desired molecules that can be of significant industrial applications. These microbes can be a useful alternative for sustainable solutions for ecological control of pests and diseases, and can reduce the burden of excess of chemical fertilizers for this purpose. This book is an attempt to review the recent development in the understanding of microbial endophytes and their potential biotechnological applications. This is a collection of literature authored by noted researchers having signatory status in endophytic research and summarizes the development achieved so far, and future prospects for further research in this fascinating area of research."

Studying narratives is often the best way to gain a good understanding of how various aspects of human information are organized and integrated-the narrator employs specific informational methods to build the whole structure of a narrative through combining temporally constructed events in light of an array of relationships to the narratee and these methods reveal the interaction of the rational and the sensitive aspects of human information. Computational and Cognitive Approaches to Narratology discusses issues of narrative-related information and communication technologies, cognitive mechanism and analyses, and theoretical perspectives on narratives and the story generation process. Focusing on emerging research as well as applications in a variety of fields including marketing, philosophy, psychology, art, and literature, this timely publication is an essential reference source for researchers, professionals, and graduate students in various information technology, cognitive studies, design, and creative fields.

A brain-computer interface (BCI) establishes a direct output channel between the human brain and external devices. BCIs infer user intent via direct measures of brain activity and thus enable communication and control without movement. This book, authored by experts in the field, provides an accessible introduction to the neurophysiological and signal-processing background required for BCI, presents state-of-the-art non-invasive and invasive approaches, gives an overview of current hardware and software solutions, and reviews the most interesting as well as new, emerging BCI applications. The book is intended not only for students and young researchers, but also for newcomers and other readers from diverse backgrounds keen to learn about this vital scientific endeavour.

Foreword by Walter J. Freeman.

The induction of unconsciousness using anesthetic agents demonstrates that the cerebral cortex can operate in two very different behavioral modes: alert and responsive vs. unaware and quiescent. But the states of wakefulness and sleep are not single-neuron properties---they emerge as bulk properties of cooperating populations of neurons, with the switchover between states being similar to the physical change of phase observed when water freezes or ice melts. Some brain-state transitions, such as sleep cycling, anesthetic induction, epileptic seizure, are obvious and detected readily with a few EEG electrodes; others, such as the emergence of gamma rhythms during cognition, or the ultra-slow BOLD rhythms of relaxed free-association, are much more subtle. The unifying theme of this book is the notion that all of these bulk changes in brain behavior can be treated as phase transitions between distinct brain states.

Modeling Phase Transitions in the Brain contains chapter contributions from leading researchers who apply state-space methods, network models, and biophysically-motivated continuum approaches to investigate a range of neuroscientifically relevant problems that include analysis of nonstationary EEG time-series; network topologies that limit epileptic spreading; saddle--node bifurcations for anesthesia, sleep-cycling, and the wake--sleep switch; prediction of dynamical and noise-induced spatiotemporal instabilities underlying BOLD, alpha-, and gamma-band Hopf oscillations, gap-junction-moderated Turing structures, and Hopf-Turing interactions leading to cortical waves.

Since information in the brain is processed by the exchange of spikes among neurons, a study of such group dynamics is extremely important in understanding hippocampus dependent memory. These spike patterns and local field potentials (LFPs) have been analyzed by various statistical methods. These studies have led to important findings of memory information processing. For example, memory-trace replay, a reactivation of behaviorally induced neural patterns during subsequent sleep, has been suggested to play an important role in memory consolidation. It has also been suggested that a ripple/sharp wave event (one of the characteristics of LFPs in the hippocampus) and spiking activity in the cortex have a specific relationship that may facilitate the consolidation of hippocampal dependent memory from the hippocampus to the cortex. The book will provide a state-of-the-art finding of memory information processing through the analysis of multi-neuronal data. The first half of the book is devoted to this analysis aspect. Understanding memory information representation and its consolidation, however, cannot be achieved only by analyzing the data. It is extremely important to construct a computational model to seek an underlying mathematical principle. In other words, an entire picture of hippocampus dependent memory system would be elucidated through close collaboration among experiments, data analysis, and computational modeling. Not only does computational modeling benefit the data analysis of multi-electrode recordings, but it also provides useful insight for future experiments and analyses. The second half of the book will be devoted to the computational modeling of hippocampus-dependent memory.

This book is about evolutionary theory. It deals with aspects of its history to focus upon explanatory structures at work in the various forms of evolutionary theory - as such this is also a work of philosophy. Its focus lies on recent debates about the Modern Synthesis and what might be lacking in that synthesis. These claims have been most clearly made by those calling for an Extended Evolutionary Synthesis. The author argues that the difference between these two positions is the consequence of two things. First, whether evolution is a considered as solely a population level phenomenon or also a theory of form. Second, the use of information concepts. In this book Darwinian evolution is positioned as a general theory of evolution, a theory that gave evolution a technical meaning as the statistical outcome of variation, competition, and inheritance. The Modern Synthesis (MS) within biology, has a particular focus, a particular architecture to its explanations that renders it a special theory of evolution. After providing a history of Darwinian theory and the MS, recent claims and exhortations for an Extended Evolutionary Synthesis (EES) are examined that see the need for the inclusion of non-genetic modes of inheritance and also developmental processes. Much of this argument is based around claims that the MS adopts a particular view of information that has privileged the gene as an instructional unit in the emergence of form. The author analyses the uses of information and claims that neither side of the debate explicitly and formally deals with this concept. A more formal view of information is provided which challenges the EES claims about the role of genes in MS explanations of form whilst being consilient with their own interests in developmental biology. It is concluded that the MS implicitly assumed this formal view of information whilst using information terms in a colloquial manner. In the final chapter the idea that the MS is an informational theory that acts to corral more specific phenomenal accounts, is mooted. As such the book argues for a constrained pluralism within biology, where the MS describes those constraints.

Changes in atmospheric carbon dioxide concentrations and global climate conditions have altered photosynthesis and plant respiration across both geologic and contemporary time scales. Understanding climate change effects on plant carbon dynamics is critical for predicting plant responses to future growing conditions. Furthermore, demand for biofuel, fibre and food production is rapidly increasing with the ever-expanding global human population, and our ability to meet these demands is exacerbated by climate change. This volume integrates physiological, ecological, and evolutionary perspectives on photosynthesis and respiration responses to climate change. We explore this topic in the context of modeling plant responses to climate, including physiological mechanisms that constrain carbon assimilation and the potential for plants to acclimate to rising carbon dioxide concentration, warming temperatures and drought. Additional chapters contrast climate change responses in natural and agricultural ecosystems, where differences in climate sensitivity between different photosynthetic pathways can influence community and ecosystem processes. Evolutionary studies over past and current time scales provide further insight into evolutionary changes in photosynthetic traits, the emergence of novel plant strategies, and the potential for rapid evolutionary responses to future climate conditions. Finally, we discuss novel approaches to engineering photosynthesis and photorespiration to improve plant productivity for the future. The overall goals for this volume are to highlight recent advances in photosynthesis and respiration research, and to identify key challenges to understanding and scaling plant physiological responses to climate change. The integrated perspectives and broad scope of research make this volume an excellent resource for both students and researchers in many areas of plant science, including plant physiology, ecology, evolution, climate change, and biotechnology. For this volume, 37 experts contributed chapters that span modeling, empirical, and applied research on photosynthesis and respiration responses to climate change. Authors represent the following seven countries: Australia (6); Canada (9), England (5), Germany (2), Spain (3), and the United States (12).

Michael P. Richards and Jean-Jacques Hublin The study of hominin diets, and especially how they have (primates, modern humans), (2) faunal and plant studies, (3) evolved throughout time, has long been a core research archaeology and paleoanthropology, and (4) isotopic studies. area in archaeology and paleoanthropology, but it is also This volume therefore presents research articles by most of becoming an important research area in other fields such as these participants that are mainly based on their presentations primatology, nutrition science, and evolutionary medicine. at the symposium. As can hopefully be seen in the volume, Although this is a fundamental research topic, much of the these papers provide important reviews of the current research research continues to be undertaken by specialists and there in these areas, as well as often present new research on dietary is, with some notable exceptions (e. g. , Stanford and Bunn, evolution. 2001; Ungar and Teaford, 2002; Ungar, 2007) relatively lit- In the section on modern studies Hohmann provides a tle interaction with other researchers in other fields. This is review of the diets of non-human primates, including an unfortunate, as recently it has appeared that different lines interesting discussion of the role of food-sharing amongst of evidence are causing similar conclusions about the major these primates. Snodgrass, Leonard, and Roberston provide issues of hominid dietary evolution (i. e.

This is a reference handbook for young researchers exploring gene and cell therapy. Gene therapy could be defined as a set of strategies modifying gene expression or correcting mutant/defective genes through the administration of DNA (or RNA) to cells, in order to treat disease. Important advances like the discovery of RNA interference, the completion of the Human Genome project or the development of induced pluripotent stem cells (iPSc) and the basics of gene therapy are covered. This is a great book for students, teachers, biomedical researchers delving into gene/cell therapy or researchers borrowing skills from this scientific field.