Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments.

This resource demonstrates how a combination of modern techniques is used to ensure that horseracing is both fair and prevents abuse of the horses involved. Based on the work of the Horseracing Forensic Laboratory (HFL) located near Newmarket in the UK, the book comprises five sections of student material. First, an overview of the work of HFL is presented, followed by sections on immunoassay, metabolism and chromatography. Teachers' notes are also included. Following the explanatory text are questions, which assist with understanding and also illustrate real-life applications of the chemical techniques encountered at school. Chemistry at the Races is designed mostly for ages 16+, but some material is also included for younger students. It is an invaluable resource for teachers, enabling them to demonstrate an up-to-date and interesting context for their work.

High-Throughput Mass Spectrometry in Drug Discovery Apply mass spectrometry to every phase of new drug discovery with this cutting-edge guide Mass spectrometry is a technique that identifies and characterizes compounds based on their mass — the fundamental molecular characteristic. It has become an invaluable analytical tool in various disciplines, industries, and research fields. It has become particularly central to new drug discovery and development, which broadly deploys mass spectrometry at every phase. The pharmaceutical industry has become one of the main drivers of technological development in mass spectrometry. High-Throughput Mass Spectrometry in Drug Discovery offers a comprehensive introduction to mass spectrometry and its applications in pharmaceutical discovery. It covers the foundational principles and science of mass spectrometry before moving to specific experimental methods and their applications at various stages of drug discovery. Its thorough treatment and detailed guidance make it an invaluable tool for pharmaceutical research and development. High-Throughput Mass Spectrometry in Drug Discovery readers will also find: Detailed analysis of techniques, including label-free screening, synthetic reaction optimization, and more An authorial team with extensive combined experience in research and industrial applications Technical strategies with the potential to accelerate quantitative bioanalysis in drug discovery High-Throughput Mass Spectrometry in Drug Discovery is essential for analytical, bioanalytical, and medicinal chemists working in the pharmaceutical industry and for any researchers and graduate students interested in drug discovery and development.

This volume is devoted to compiling recent advancements, methodological improvements, new processing techniques, integration methods and rigorous applications associated with conceptual techniques on the conservation and monitoring of forest resources for a scientific audience, with a focus on cases and applications in India. The primary objective of the book is to advance the scientific understanding of the recent trends and technological improvements in forest conservation, management and related research themes in forest resources and human-wildlife interactions. The book is organized into five sections: (I) Forest Conservation Ecology (II) Forest Conservation and Society (III) Forest Management (IV) Forest Monitoring using GIS and Remote Sensing and (V) Human Wildlife Conflicts. It covers various research themes related to forestry, wildlife, habitat fragmentation, forest management and human-wildlife conflict research, and therefore will be beneficial to a diverse range of researchers, scientific organizations, wildlife scientists, biologists, ecologists and planners in the fields of wildlife and forestry. The book will further be of use to post-graduates, PhD research scholars, professors, geospatial experts, modellers, foresters, agricultural scientists, biologists, ecologists, environmental consultants and big data compilers.

In this thesis Johanna Bruckner reports the discovery of the lyotropic counterpart of the thermotropic SmC* phase, which has become famous as the only spontaneously polarized, ferroelectric fluid in nature. By means of polarizing optical microscopy, X-ray diffraction and electro-optic experiments she firmly establishes aspects of the structure of the novel lyotropic liquid crystalline phase and elucidates its fascinating properties, among them a pronounced polar electro-optic effect, analogous to the ferroelectric switching of its thermotropic counterpart. The helical ground state of the mesophase raises the fundamental question of how chiral interactions are "communicated" across layers of more or less disordered and achiral solvent molecules which are located between adjacent bi-layers of the chiral amphiphile molecules. This thesis bridges an important gap between thermotropic and lyotropic liquid crystals and pioneers a new field of liquid crystal research.

This 8-volume set provides a systematic description on 8,350 active marine natural products from 3,025 various kinds of marine organisms. The diversity of structures, biological resources and pharmacological activities are discussed in detail. Molecular structural classification system with 264 structural types are developed. The 4th volume continuously illustrates the molecular formula and structures of alkaloids.

Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins.

This book explains how the partial differential equations (pdes) in electroanalytical chemistry can be solved numerically. It guides the reader through the topic in a very didactic way, by first introducing and discussing the basic equations along with some model systems as test cases systematically. Then it outlines basic numerical approximations for derivatives and techniques for the numerical solution of ordinary differential equations. Finally, more complicated methods for approaching the pdes are derived. The authors describe major implicit methods in detail and show how to handle homogeneous chemical reactions, even including coupled and nonlinear cases. On this basis, more advanced techniques are briefly sketched and some of the commercially available programs are discussed. In this way the reader is systematically guided and can learn the tools for approaching his own electrochemical simulation problems. This new fourth edition has been carefully revised, updated and extended compared to the previous edition (Lecture Notes in Physics Vol. 666). It contains new material describing migration effects, as well as arrays of ultramicroelectrodes. It is thus the most comprehensive and didactic introduction to the topic of electrochemical simulation.

The thesis provides the necessary experimental and analytical tools to unambiguously observe the atomically resolved chemical reactions. A great challenge of modern science has been to directly observe atomic motions during structural transitions, and while this was first achieved through a major advance in electron source brightness, the information content was still limited and new methods for image reconstruction using femtosecond electron diffraction methods were needed. One particular challenge lay in reconciling the innumerable possible nuclear configurations with the observation of chemical reaction mechanisms that reproducibly give the same kind of chemistry for large classes of molecules. The author shows that there is a simple solution that occurs during barrier crossing in which the highly anharmonic potential at that point in nuclear rearrangements couples high- and low-frequency vibrational modes to give highly localized nuclear motions, reducing hundreds of potential degrees of freedom to just a few key modes. Specific examples are given in this thesis, including two photoinduced phase transitions in an organic system, a ring closure reaction, and two direct observations of nuclear reorganization driven by spin transitions. The emerging field of structural dynamics promises to change the way we think about the physics of chemistry and this thesis provides tools to make it happen.

Solid Phase Microextraction (SPME) is a flexible and convenient sampling and sample preparation technique that extracts different kinds of analytes, including both volatile and non-volatile, without the use of a solvent. The technique facilitates fast, simple and automated determination of target analytes in a range of matrices. As it offers a green methodology, it is growing in popularity as an alternative tool in analytical chemistry to traditional methods. This book follows on in spirit from the editors' previous title, Applications of Solid Phase Microextraction and will introduce the reader to breakthrough methodologies and cutting edge applications. Although it assumes a good degree of SPME knowledge, an overview of the fundamentals is given before taking the reader through an update of the field. The reader will learn the basic principles and advantages of different SPME formats including the stir bar extraction techniques, thin film SPME, Bio-SPME, and new trends in different coatings. Applications in complex media, including food analysis, drug residues and bioanalysis are covered. Bringing together leading sample preparation academics from around the world, the editor has put together an informative new book, suitable for analytical chemists and practitioners utilising SPME tools in their research.

Principles of Analytical Chemistry aims to ease the first contact of students pursuing different scientific and technical studies by providing them with a simple, general overview of the discipline. The objective of this innovative textbook is to teach rather than to inform. Using keywords of modern Analytical Chemistry, it constructs a teaching message accessible to the beginner, with emphasis on relations between these keywords to ensure consistency in the teaching contents of the book. Practical exercises, 450 questions for students, and 27 interactive seminars are used as vehicles for expounding a large number of examples in order to clarify and consolidate the text and facilitate its comprehension.

More than one and a half decades have passed since the last book was published describing developments in the analytical chemistry of synthetic colorants. In the intervening period, the scope and technical capabilities of instrumentation for analysing dyes and pigments has significantly expanded. It is now possible to rapidly resolve a number of problems whose solutions were previously either unattainable or very difficult to achieve. For instance, the unambiguous assignment of all the signals in the proton NMR spectrum of a trisazo direct dye, and the confirmation of the molecular weight of involatile, and, in particular, sulphonated dyes, without derivatisation, are now routine analytical techniques in many laboratories today. In addition, it is now possible to record the NMR spectrum of a dye molecule on less than 1 mg of material, and we are no longer limited to solution spectra, since solid samples can now be routinely analysed in NMR experiments. Whilst not attempting to be all encompassing, this volume is intended to bridge the gap between what was covered in the earlier work edited by Professor Venkataraman and the developments which have since ensued in some key areas. It provides important updates in X-ray crystallography, proton NMR, IR spectroscopy and mass spectrometry, and additionally covers topics such as ESR, micro spectrophotometry and emission spectroscopy.

This book presents quality technical papers representing the recent developments in the field of hydrological modeling, water management and water governance including practical applications. The content covers multifarious aspects of hydrology and water resources. It includes an application of the Hydrologic Modelling System (HEC-HMS) which has been successfully demonstrated for assessment of floods. The authors suggest an approach for the mitigation of cyclone disaster through a case study of the Phailin cyclone, whilst considering mitigating pluvial flooding, developing suitable management strategies. The book includes chapters discussing the detrended fluctuation analysis which is carried out for multifractal description of droughts. Drought characteristics are analyzed, and drought indices evolved for drought preparedness/management. The use of science in community planning under changing climate is also studied and discussed. The authors present and experimental study wherein hydraulic coefficients are calibrated by using vertical orifice. A cross flow hybrid hydrokinetic turbine is also evaluated for performance, and high head regulating radial gate designed and studied its sensitivity. This book will appeal to researchers, field practitioners, NGO and other Governmental as well as private water practitioners

This book presents innovative research on soil nitrogen cycling and nitrate leaching with a view to improving soil management and fertiliser nitrogen use efficiency and reducing nitrogen leaching losses. In this regard, nitrogen-15 (15N)-labelled fertiliser was used as a biochemical and physical stable isotope tracer in laboratory and field experiments. The major outcome of the research was the development, validation and application of a new compound-specific amino acid 15N stable isotope probing method for assessing the assimilation of fertiliser nitrogen by soil microbial biomass. The novelty of the method lies in its tracing of incorporated nitrogen into newly biosynthesised microbial protein in time-course experiments using gas chromatography-combustion-isotope ratio mass spectrometry. The approach provides previously unattainable insights into the microbial processing of different nitrogen fertilisers in different soils. Further, it identifies the mechanistic link between molecular-scale processes and observations of field-scale fertiliser nitrogen immobilisation studies. The method and the results presented here will have far-reaching implications for the development of enhanced recommendations concerning farm-based soil management practices for increasing soil productivity and reducing nitrogen losses, which is essential to minimising environmental impacts.

This volume summarises recent developments and highlights new techniques which will define possible future directions for small molecule X-ray crystallography. It provides an insight into how specific aspects of crystallography are developing and shows how they may interact or integrate with other areas of science. The development of more sophisticated equipment and the massive rise in computing power has made it possible to solve the three-dimensional structure of an organic molecule within hours if not minutes. This successful trajectory has resulted in the ability to study ever more complex molecules and use smaller and smaller crystals. The structural parameters for over a million organic and organometallic compounds are now archived in the most commonly used database and this wealth of information creates a new set of problems for future generations of scientists. The volume provides some insight into how users of crystallographic structural data banks can navigate their way through a world where "big data" has become the norm. The coupling of crystallography to quantum chemical calculations provides detailed information about electron distributions in crystals affording a much more detailed analysis of bonding than has been possible previously. In quantum crystallography, quantum mechanical wavefunctions are used to extract information about bonding and properties from the measured X-ray structure factors. The advent of quantum crystallography has resulted in form and structure factors derived from quantum mechanics which have been used in advanced refinement and wavefunction fitting. This volume describes how quantum mechanically derived atomic form factors and structure factors are constructed to allow the improved description of the diffraction experiment. It further discusses recent developments in this field and illustrates their applications with a wide range of examples. This volume will be of interest to chemists and crystallographers with an interest in the synthesis, characterisation and physical and catalytic properties of solid-state materials. It will also be relevant for the community of computational chemists who study chemical systems. Postgraduate students entering the field will benefit from a historical introduction to the way in which scientists have used the data derived from crystallography to develop new structural and bonding models.

The introduction of contaminants, due to rapid urbanisation and anthropogenic activities, into the environment causes unsteadiness, distress to the physico-chemical systems including living organisms, which possibly is threatening the dynamics of nature as well as the soil biology by producing certain xenobiotics. Hence, there is an immediate global demand for the diminution of such contaminants and xenobiotics which can otherwise adversely affect the living organisms. Some toxic xenobiotics include synthetic organochlorides such as polycyclic aromatic hydrocarbons (PAHs), and some fractions of crude oil and coal. The advancements in microbiology and biotechnology has lead to the launch of microbial biotechnology as a separate area of research and contributed dramatically to the development of the areas like agriculture, environment, biopharmaceutics, fermented foods, etc. The evolution of new metabolic pathways from natural metabolic cycles has enabled the microorganisms to degrade almost all different complex and resistant xenobiotics found on Earth. Hence, microbes stand an imperative, efficient, green and economical alternative to conventional treatment technologies. This book comprises chapters dealing with various bioremediation strategies with the help of different groups of microorganisms along with detailed graphical/ diagrammatical representations. It also focuses on the use of microbial biotechnology and highlights the recent developments in microbial biotechnology in the area of agriculture and environment. Furthermore, it contains a detailed comprehensive account for the microbial treatment technologies from unsustainable to sustainable which includes chapters prepared by professionals, several researchers, scientists, graduate students and postdoctoral fellows across the world with expertise in environmental microbiology, biotechnology, bioremediation, and environmental engineering. The research presented also highlights some of the significantly important microbial species involved in remediation, the physiology, biochemistry and the mechanisms of remediation by various microbes, and suggestions for future improvement of bioremediation technology. This book would serve as a quick reference book for graduate and postgraduate students pursuing their study in any branch of life sciences, microbiology, health sciences and environmental biotechnology as well as researchers and scientists working in laboratories and industries involved in research related to microbiology, environmental biotechnology and allied researches.

This book describes current understandings and recent progress in four areas: in the first one, the cytochalasans, a group of fungal derived natural products characterized by a perhydro-isoindolone core fused with a macrocyclic ring are shown to exhibit high structural diversity and a broad spectrum of bioactivities. The second one is dedicated to a description of bioactive compounds from the medicinal plants of Myanmar, the third one is dedicated to new structure elucidation techniques in the field of sesquiterpenes. The last one discusses the endogenous natural products that are produced by human cells including endogenous amines, steroids, and fatty acid derived natural products. The co-metabolism and natural product production of the human microbiome is also described including tryptophan, bile acids, choline, and cysteine.

Covers material characterization techniques and the development of advanced characterization technology Includes multiple length scale characterization approaches for a large variety of materials, from nano- to micron-scale, as well as their constraints Discusses advanced material characterization technology in the microstructural and property characterization fields Reviews both practical and theoretical explanations of approaches for characterizing microstructure and properties Offers fundamentals, basic instrumentation details, experimental approaches, analyses, and applications with case studies

This open access book is a pedagogical, examples-based guide to using the Monte Carlo N-Particle (MCNP (R)) code for nuclear safeguards and non-proliferation applications. The MCNP code, general-purpose software for particle transport simulations, is widely used in the field of nuclear safeguards and non-proliferation for numerous applications including detector design and calibration, and the study of scenarios such as measurement of fresh and spent fuel. This book fills a gap in the existing MCNP software literature by teaching MCNP software usage through detailed examples that were selected based on both student feedback and the real-world experience of the nuclear safeguards group at Los Alamos National Laboratory. MCNP input and output files are explained, and the technical details used in MCNP input file preparation are linked to the MCNP code manual. Benefiting from the authors' decades of experience in MCNP simulation, this book is essential reading for students, academic researchers, and practitioners whose work in nuclear physics or nuclear engineering is related to non-proliferation or nuclear safeguards. Each chapter comes with downloadable input files for the user to easily reproduce the examples in the text.

This Second edition of the flow injection analysis (FIA) text which has become a standard in the field in four languages, is written by two pioneers in the field, who have themselves discovered many of the techniques and designed much of the equipment employed in FIA. Newly revised to account for the many recent developments in FIA, this book presents the state--of--the--art in FIA theory, techniques, and future trends. Specific topics covered include continous--flow analyzers, chemical kinetics in an FIA system, theory of dispersion related to FIA, single--line FIA manifolds, FIA determinations based on separation processes, commercially available flow--injection analyzers, the FIA laboratory----a microconduit--based pedagogical system, review of the flow--injection literature, and flow injection analysis now and in the future.

This volume focuses on modelling the fate of chemicals in the environment and the human body to arrive at an integrated exposure assessment. It covers five broad topics, namely: future challenges in exposure assessment; the evolution of human health and environmental risk assessment; standard documentation for exposure models; modelling different environmental components (i.e. surface waters, atmosphere, soil, groundwater, plants, aquatic organisms and mammals); and the fate of contaminants in humans. This work draws on the authors' and editors' extensive experience and a range of different research activities, including case studies, that have led to the development of MERLIN-Expo, a standardised software package for simulating the fate of chemicals in the main environmental systems and in the human body in an integrated manner. It will be of considerable interest to researchers and students, risk managers, and policy- and decision-makers whose work involves environmental protection and human health.

In recent years, consumers have been concentrating on the health benefits and nutrition from food to preserve a healthy lifestyle. They are looking for colorants derived from natural sources to enhance the nutritional and antioxidant value of foods. Coloured cereals (wheat, rice, barley, oat, maize, sorghum, and millets) contain many phytochemicals, including anthocyanins and carotenoids associated with numerous health benefits. This book presents a comprehensive overview of the bioactive potential, food applications, and health benefits of coloured cereal grains. Novel approaches to the integration of coloured grain into food in the food processing industries are included, reviewing high-value pigments in the bran layer which can easily be extracted and utilized as functional foods and natural colorants. Aimed at researchers carrying out innovative studies, food regulatory and safety authorities and food processing industries who are trying to minimize synthetic food colorants and dyes, this book provides a novel approach to the use of substitute synthetic dyes which can improve the nutritional value, appearance, texture, flavour, and storage properties of food products.

The semiconductor industry is moving toward gas-phase reagents, increasing the relative importance of gas purity. Anyone who deals in the manufacturing of these devices needs to understand the technology available for modern gas analysis. Most specialty gas vendors have some re in place for quality assurance, but these usually are very simplistic and outdated methods. No book was available that gave guidance on providing accurate, reproducible data on specialty gas products. This is the first book that provides an introduction to current analytical methods and equipment for the analysis of high- purity gases used in the semiconductor industry and related fields.