This manual not only provides reliable, up-to-date protocols for lab use but also the theoretical background of molecular biology, allowing users to better understand the principles underlying these techniques. It covers a wide range of methods, including the purification of nucleic acids, enzymatic modification of DNA, isolation of specific DNA fragments, PCR, cloning techniques, and gene expression. A Springer Lab Manual

Researchers have long had an interest in dental morphology as a genetic proxy to reconstruct population history. Much interest was fostered by the use of standard plaques and associated descriptions that comprise the Arizona State University Dental Anthropology System, developed by Christy G. Turner, II and students. This system has served as the foundation for hundreds of anthropological studies for over 30 years. In recognition of that success, this volume brings together some of the world's leading dental morphologists to expand upon the concepts and methods presented in the popular The Anthropology of Modern Human Teeth (Cambridge University Press, 1997), leading the reader from method to applied research. After a preparatory section on the current knowledge of heritability and gene expression, a series of case studies demonstrate the utility of dental morphological study in both fossil and more recent populations (and individuals), from local to global scales.

Few things are as interesting to us as our own bodies and, by extension, our own identities. In recent years, there has been a growing interest in the relationship between the body, environment and society. Reflecting upon these developments, this book examines the role of the body in human identification, in the forging of identities, and the ways in which it embodies our social worlds. The approach is integrative, taking a uniquely biological perspective and reflecting on current discourse in the social sciences. With particular reference to bioarchaeology and forensic science, the authors focus on the construction and categorisation of the body within scientific and popular discourse, examining its many tissues, from the outermost to the innermost, from the skin to DNA. Synthesising two, traditionally disparate, strands of research, this is a valuable contribution to research on human identification and the embodiment of identity.

The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signaling molecules as well as many pathophysiological processes, including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles, and in the immediate surroundings of individual cells are described in practical detail by experts. Also included is a chapter on tracking calmodulin inside cells using fluorescence technology. A Springer Lab Manual

The emergence of the genus Homo is widely linked to the colonization of 'new' highly seasonal savannah habitats. However, until recently, our understanding of the possible impact of seasonality on this shift has been limited because we have little general knowledge of how seasonality affects the lives of primates. This book documents the extent of seasonality in food abundance in tropical woody vegetation, and then presents systematic analyses of the impact of seasonality in food supply on the behavioural ecology of non-human primates. Syntheses in this volume then produce broad generalizations concerning the impact of seasonality on behavioural ecology and reproduction in both human and non-human primates, and apply these insights to primate and human evolution. Written for graduate students and researchers in biological anthropology and behavioural ecology, this is an absorbing account of how seasonality may have affected an important episode in our own evolution.

Biolinguistics is a highly interdisciplinary field that seeks the rapprochement between linguistics and biology. Linking theoretical linguistics, theoretical biology, genetics, neuroscience and cognitive psychology, this book offers a collection of chapters situating the enterprise conceptually, highlighting both the promises and challenges of the field, and chapters focusing on the challenges and prospects of taking interdisciplinarity seriously. It provides concrete illustrations of some of the cutting-edge research in biolinguistics and piques the interest of undergraduate students looking for a field to major in and inspires graduate students on possible research directions. It is also meant to show to specialists in adjacent fields how a particular strand of theoretical linguistics relates to their concerns, and in so doing, the book intends to foster collaboration across disciplines.

An overview of biomechanical modeling of human soft tissue using nonlinear theoretical mechanics and incremental finite element methods, useful for computer simulation of the human musculoskeletal system.

This book offers a clearly written, entertaining and comprehensive source of medical information for both writers and readers of science fiction. Science fiction in print, in movies and on television all too often presents dubious or simply incorrect depictions of human biology and medical issues. This book explores the real science behind such topics as how our bodies adapt to being in space, the real-life feasibility of common plot elements such as suspended animation and medical nanotechnology, and future prospects for improving health, prolonging our lives, and enhancing our bodies through technology. Each chapter focuses on a single important science fiction-related subject, combining concise factual information with examples drawn from science fiction in all media. Chapters conclude with a "Bottom Line" section summarizing the most important points discussed in the chapter and giving science fiction writers practical advice on how to incorporate them into their own creations, including a list of references for further reading. The book will appeal to all readers interested in learning about the latest ideas on a variety of science fiction-related medical topics, and offers an invaluable reference source for writers seeking to increase the realism and readability of their works. Henry G. Stratmann, MD, FACC, FACP is a cardiologist with board certifications in internal medicine, cardiology, and nuclear cardiology. Befor e entering private practice he became Professor of Medicine at St. Louis University School of Medicine and performed clinical medical research. Henry received a BA in chemistry from St. Louis University and his MD at Southern Illinois University School of Medicine. He is currently enrolled at Missouri State University to obtain a BS in physics with a minor in astronomy. His professional publications include being an author or coauthor of many research articles for medical journals, primarily in the field of nuclear cardiology. Henry is also a regular contributor of both stories and science fact articles to Analog Science Fiction and Fact.

This book provides a unique discussion of human evolution from a philosophical viewpoint, looking at the facts and interpretations since Charles Darwin's The Descent of Man. Michael Ruse explores such topics as the nature of scientific theories, the relationships between culture and biology, the problem of progress and the extent to which evolutionary issues pose problems for religious beliefs. He identifies these issues, highlighting the problems for morality in a world governed by natural selection. By taking a philosophical viewpoint, the full ethical and moral dimensions of human evolution are examined. This book engages the reader in a thorough discussion of the issues, appealing to students in philosophy, biology and anthropology.

The development of populations over time, and, on longer timescales, the evolution of species, are both influenced by a complex of interacting, underlying processes. Computer simulation provides a means of experimenting within an idealised framework to allow aspects of these processes and their interactions to be isolated, controlled, and understood. In this 2005 book, computer simulation is used to model migration, extinction, fossilisation, interbreeding, selection and non-hereditary effects in the context of human populations and the observed distribution of fossil and current hominoid species. The simulations described enable the visualisation and study of lineages, genetic diversity in populations, character diversity across species and the accuracy of reconstructions, allowing insights into human evolution and the origins of humankind for graduate students and researchers in the fields of physical anthropology, human evolution, and human genetics.

Tissue engineering is a new field of biomedical engineering, in which synthetic materials are used together with biological components such as tissue fragments, cells, proteins to encourage tissue regeneration, regrowth, and repair. This book introduces the principles of tissue engineering in a unique way that is ideally suited for the modern engineering student. A review of developmental biology is presented early in the book, since biological development is the fundamental process of most relevance for tissue engineering. The study of development provides a pathway to basic bioengineering units on cell adhesion, migration, assembly, and transport, which are integrated in the final sections of the book into tissue engineering processes (such as cell delivery, growth factor administration, and polymeric scaffold materials).

In order to gain an understanding of the dynamics of human individual and average growth patterns it is essential that the right methods are selected. There are a variety of methods available to analyse individual growth patterns, to estimate variation in different growth measures in populations and to relate genetic and environmental factors to individual and average growth. This volume provides an overview of modern techniques for the assessment and collection of growth data and methods of analysis for individual and population growth data. The book contains the basic mathematical and statistical tools required to understand the concepts of the methods under discussion and worked examples of analyses, but it is neither a mathematical treatise, nor a recipe book for growth data analysis. Aimed at junior and senior researchers involved in the analysis of human growth data, this book will be an essential reference for anthropologists, auxologists and paediatricians.

In the space of one generation major changes have begun to take place in the field of human reproduction. A rapid increase in the control of fertility and the understanding and treatment of sexual health issues have been accompanied by an emerging threat to reproductive function linked to increasing environmental pollution and dramatic changes in lifestyle. Organised around four key themes, this book provides a valuable review of some of the most important recent findings in human reproductive ecology. Major topics include the impact of the environment on reproduction, the role of physical activity and energetics in regulating reproduction, sexual maturation and ovulation assessment and demographic, health and family planning issues. Both theoretical and practical issues are covered, including the evolution and importance of the menopause and the various statistical methods by which researchers can analyse characteristics of the menstrual cycle in field studies.

It is generally accepted that the earliest human ancestors grew more like apes than like humans today. If they did so, and we are now different, when, how and why did our modern growth patterns evolve? This book focuses on species within the genus Homo to investigate the evolutionary origins of characteristic human patterns and rates of craniofacial and postcranial growth and development, and to explore unique ontogenetic patterns within each fossil species. Experts examine growth patterns found within available Plio-Pleistocene hominid samples, and analyse variation in ontogenetic patterns and rates of development in recent modern humans in order to provide a comparative context for fossil hominid studies. Presenting studies of some of the newer juvenile fossil specimens and information on Homo antecessor, this book will provide a rich data source with which anthropologists and evolutionary biologists can address the questions posed above.

In this book, Benoit Dubreuil explores the creation and destruction of hierarchies in human evolution. Combining the methods of archaeology, anthropology, cognitive neuroscience and primatology, he offers a natural history of hierarchies from the point of view of both cultural and biological evolution. This volume explains why dominance hierarchies typical of primate societies disappeared in the human lineage and why the emergence of large-scale societies during the Neolithic period implied increased social differentiation, the creation of status hierarchies, and, eventually, political centralisation.

An exploration of the cosmic origins of human beings and the evolutionary laws which govern their development. Armin Husemann applies musical principles as a method of gaining insight into the structure of the human body and the forces that work on it. He draws on our experience of music and explain the physiological and anatomical relationships in the body, as well as illuminating the spiritual influences which determine physical development. Drawing on artistic exercises set out by Rudolf Steiner to develop a better understanding of these influences, the book explores the cosmic origins of human beings and the evolutionary laws which govern their development.

Origins of Language: A Slim Guide offers a concise and accessible overview of what is known about the evolution of the human capacity for language. Non-human animals communicate in simple ways: they may be able to form simple concepts, to feel some limited empathy for others, to cooperate to some extent, and to engage in mind-reading. Human language, however, is characterized by its ability to efficiently express a wide range of subtle and complex meanings. After the first simple beginnings, human language underwent an explosion of complexity, leading to the very complicated systems of grammar and pronunciation found in modern languages. Jim Hurford looks at the very varied aspects of this evolution, covering human prehistory; the relation between instinct and learning; biology and culture; trust, altruism, and cooperation; animal thought; human and non-human vocal anatomy; the meanings and forms of the first words; and the growth of complex systems of grammar and pronunciation. Written by an internationally recognized expert in the field, it draws on a number of disciplines besides linguistics, including philosophy, neuroscience, genetics, and animal behaviour, and will appeal to a wide range of readers interested in language origins and evolution.

This book looks at how the human brain got the capacity for language and how language then evolved. Its four parts are concerned with different views on the emergence of language, with what language is, how it evolved in the human brain, and finally how this process led to the properties of language. Part I considers the main approaches to the subject and how far language evolved culturally or genetically. Part II argues that language is a system of signs and considers how these elements first came together in the brain. Part III examines the evidence for brain mechanisms to allow the formation of signs. Part IV shows how the book's explanation of language origins and evolution is not only consistent with the complex properties of languages but provides the basis for a theory of syntax that offers insights into the learnability of language and to the nature of constructions that have defied decades of linguistic analysis, including including subject-verb inversion in questions, existential constructions, and long-distance dependencies. Denis Bouchard's outstandingly original account will interest linguists of all persuasions as well as cognitive scientists and others interested in the evolution of language.

There is a significant amount of research that substantiates the connection between social support/relationships and the development, onset, and/or recovery of several physical diseases/illnesses. Research has shown, for example, that an unhappy marriage can increase the likelihood of becoming ill by 35% while stressful communication can lead to an increase in cardiovascular reactivity which in turn increases the risk of coronary heart disease and premature mortality. This volume provides a comprehensive overview of the influences of communication on physiology and physical health status occurring in a variety of contexts, from families, interpersonal relationships, and public speaking to sport fandom, affection, fear, and the escalation of conflict. It offers a broad and up-to-date review of the relevant literature in this area of study.

First published in 1981, Professor Tanner's volume traces the history of studies of the physical growth of children from the time of the Ancient Greeks onwards. The author summarises the background to and the achievement of the surveys of child growth made in the course of social reform throughout the nineteenth century, and shows their relevance for social and economic history. These are studies to which the author himself made outstanding contributions and the text shows an intimate knowledge, both as to programmes and personalities. It is a unique historical record. The author not only follows the evolution of ideas that lies behind the gradual emergence of studies of growth, but also summarises the results of these studies, charting the growth of children during the nineteenth and twentieth centuries. This book will be of value for medical, social and economic historians as well as for paediatricians and biological anthropologists.

This comprehensive synthesis of current medical and evolutionary literature addresses key questions about the role body fat plays in human biology. It explores how body energy stores are regulated, how they develop over the life-course, what biological functions they serve, and how they may have evolved. There is now substantial evidence that human adiposity is not merely a buffer against the threat of starvation, but is also a resource for meeting the energy costs of growth, reproduction and immune function. As such it may be considered as important in our species evolution as other traits such as bipedalism, large brains, and long life spans and developmental periods. Indeed, adiposity is integrally linked with these other traits, and with our capacity to colonise and inhabit diverse ecosystems. It is because human metabolism is so sensitive to environmental cues that manipulative economic forces are now generating the current obesity epidemic.

Human settlement of the western fringes of the Pacific began at least 40,000 years ago. Long, hazardous sea voyages were the only way of reaching the tiny islands scattered through this vast expanse of ocean. Food and shelter were hard to come by, even on land. This book, first published in 1996, documents how these settlers adapted culturally and biologically to the distinctive Pacific environment, and how they evolved into the large-bodied, muscular people seen today in New Zealand, Polynesia, Micronesia and Melanesia. Philip Houghton considers studies of DNA, patterns of health and disease, and computer simulations of human survival at sea based on the likely routes through the Pacific. People of the Great Ocean is a unique work based on extensive research and careful analysis. Philip Houghton's text presents detailed technical information, but remains highly readable and persuasive.

Neanderthals and Modern Humans develops the theme of the close relationship between climate change, ecological change and biogeographical patterns in humans during the Pleistocene. In particular, it challenges the view that Modern Human 'superiority' caused the extinction of the Neanderthals between 40 and 30 thousand years ago. Clive Finlayson shows that to understand human evolution, the spread of humankind across the world and the extinction of archaic populations, we must move away from a purely theoretical evolutionary ecology base and realise the importance of wider biogeographic patterns including the role of tropical and temperate refugia. His proposal is that Neanderthals became extinct because their world changed faster than they could cope with, and that their relationship with the arriving Modern Humans, where they met, was subtle.

In past years considerable interest has been focused on migration as an important cause of change in the genetic and demographic structure of human populations. This book synthesises the biological consequences of changes environments on the migrants and the genetic impact of immigration on the host populations. Patterns of migration, past and present and genetic, epidemological and demographic consequences are considered, forming a unique synthesis for human biologists in general. Individual chapters deal with the peopling of the continents, migration in the recent past, the effects of gene flow and rural to urban migration. In addition, a detailed analysis of the relationship between migration, adaptation and disease is presented. Advanced students and research workers in a wide variety of disciplines, including population genetics, demography, anthropology and social geography will find this book particularly valuable in relating their own special interests to other biological aspects of human migration.

Working capacity is the physiological key to understanding man's ability, in technically less advanced communities, to exploit his environment, and hence to understanding his role in the ecological balance. In this volume the knowledge of working capacity in tropical populations is reviewed in a series of illustrative papers. Topics cover the measurement of working capacity in populations: the functional consequences of malnutrition; growth, size and muscular efficiency; ethnic differences in working capacity; energy; expenditure and endemic disease; and energy flow in tropical ecosystems. These papers and their ensuing discussions lead to a series of recommendations on studies to be incorporated in the Decade of the Tropics research programme of the International Union of Biological Sciences.