Corresponding to the content in the text, this comprehensive workbook provides a complete look at the bones, muscles, veins, glands, nerves, lymph nodes, and more of the head and neck. Detailed, high-quality images and a wealth of interactive learning exercises help reinforce challenging dental anatomy content and ensure that you fully grasp these foundational building blocks of oral health care. Comprehensive dental anatomy coverage reinforces student knowledge of all the key structures in the head and neck. Labeling and vocabulary review exercises aid students with memorization and recall. Review questions correlated with chapter coverage help students prepare for course quizzes and tests, as well as certification exams. Case studies challenge students to apply their critical thinking skills to real-world patient scenarios. Forty removable flashcards focus on body systems of the head and neck and are ideal for on-the-go study. Punched and perforated pages make it easy for students to submit workbook exercises as course assignments.

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo, and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.

Phosphoinositides play a major role in cellular signaling and membrane organization. During the last three decades we have learned that enzymes turning over phosphoinositides control vital physiological processes and are involved in the initiation and progression of cancer, inflammation, neurodegenerative, cardiovascular, metabolic disease and more. In two volumes, this book elucidates the crucial mechanisms that control the dynamics of phosphoinositide conversion. Starting out from phosphatidylinositol, a chain of lipid kinases collaborates to generate the oncogenic lipid phosphatidylinositol(3,4,5)-trisphosphate. For every phosphate group added, there are specific lipid kinases - and phosphatases to remove it. Additionally, phospholipases can cleave off the inositol head group and generate poly-phosphoinositols, which act as soluble signals in the cytosol. Volume II extends into the role of phosphoinositides in membrane organization and vesicular traffic. Endocytosis and exocytosis are modulated by phosphoinositides, which determine the fate and activity of integral membrane proteins. Phosphatidylinositol(4,5)-bisphosphate is a prominent flag in the plasma membrane, while phosphatidylinositol-3-phosphate decorates early endosomes. The Golgi apparatus is rich in phosphatidylinositol-4-phosphate, stressed cells increase phosphatidylinositol(3,5)-bisphosphate, and the nucleus has a phosphoinositide metabolism of its own. Phosphoinositide-dependent signaling cascades and the spatial organization of distinct phosphoinositide species are required in organelle function, fission and fusion, membrane channel regulation, cytoskeletal rearrangements, adhesion processes, and thus orchestrate complex cellular responses including growth, proliferation, differentiation, cell motility, and cell polarization.

Handbook of HLA Typing Techniques is an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. The information presented, much of it previously unpublished, was written by leading authorities in the field of transplantation. Each chapter provides detailed methodologies, notes on the interpretation of tests, reference material, and appendices.

Phenotyping topics covered include microlymphocytotoxicity assay, monoclonal antibodies, complement testing, serum screening for HLA antibodies, lymphocytotoxic crossmatching, and mixed and primed lymphocyte testing. Genotyping topics discussed include well-established RFLP analysis and polymerase chain reaction applications of PCR-SSO, PCR-RFLP, PCR-SSP, and PCR fingerprinting and related DNA heteroduplex technologies. Handbook of HLA Typing Techniques is an indispensable sourcebook for all researchers, practitioners, and students in the international tissue typing and human immunogenetics community.

Master the essentials of oral biology with patient-centered approach. New illustrations and discussions of emerging issues keep you updated with advances in the dental professions and patient care. Written by Daniel J. Chiego Jr., and Professor Sausan Al Kawas both are experienced educators and researchers in oral histology and embryology, this book provides the basic science you need to succeed in the classroom and in clinical practice.

This fully revised new edition explores advances in the prevention and treatment of oral diseases. Beyond the updated chapters, the book delves into regenerative biology, gene editing and the use of CRISPR in oral biology, as well as histone acetylation and deacetylation methods, further reflecting advances in the application of molecular techniques to oral biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Oral Biology: Molecular Techniques and Applications, Third Edition serves as an ideal basic resource not only for new researchers but also for experienced scientists wishing to expand their research platform into new areas of this vital field.

In the post human-genome project era, cancer specific genomic maps are redesigning tumor taxonomy by evolving from histopathology to molecular pathology. The success of a cancer drug today is fundamentally based on the success in identifying target genes that control beneficial pathways. The overwhelming power of genomics and proteomics has enlightened researchers about the fact that the PI3K-mTOR pathway is the most commonly up-regulated signal transduction pathway in various cancers, either by virtue of its activation downstream of many cell surface growth factor receptors or by virtue of its collateral and compensatory circuitry with RAS-MAPK pathway. Oncogenic signaling in the majority of solid tumors is sustained via the PI3K-AKT-mTOR pathway. Because of its prominent role in many cancer types, the PI3K-mTOR pathway has become a major therapeutic target. The volume includes two complementary parts which address the problem of etiology and disease progression and is intended to portray the very basic mechanisms of the PI3K-AKT-mTOR signaling pathway's involvement in various facets of the cancer, including stem cell renewal, cell metabolism, angiogenesis, genetic instability, and drug resistance. Significant progress has been made in recent years elucidating the molecular mechanism of cancer cell proliferation, angiogenesis, and drug-resistance in relation to the PI3K-mTOR pathway and this volume provides an in-depth overview of recent developments made in this area.

This volume provides researchers with up-to-date protocols and takes a close look at current research and promising applications. Optogenetics: Methods and Protocols contains a collection of recently developed technical protocols on optogenetic applications in neuroscience, brain mapping, treatment of neurological disorders, and restoration of visual function. Several introductory and discussion chapters offer a wide overview about sources and diversity of optogenetic tools, design strategies, and potential applications in other fields like plant research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Optogenetic: Methods and Protocols, delivers an interdisciplinary view of optogenetic applications and its potential to develop as a valuable tool for basic research, as well as biomedical and biotechnological applications.

This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.

During the last decades, research on spinal cord has attracted a great deal of attention because of problems such as sensory-motor and autonomic dysfunctions associated with traumatic and other injuries. Recording of spinal cord evoked potentials is one of the most promising approaches to understand the spinal cord function. However, the details of the evoked potentials and their significance in various pathological conditions are not yet fully characterized. This book summarizes new findings in the field of electrophysiology and relates this knowledge to pathology and regeneration research. It is the first one which deals in great detail with various ways to monitor spinal cord function in experimental and clinical situations. It provides an up-to-date knowledge regarding spinal cord bioelectrical activity and its modification with pharmacological agents and covers new aspects of regeneration studies, particularly the role of myelin associated inhibitory molecules. A section is devoted to clinical studies dealing with electrical activity, pathology, and current therapeutic measures. This volume will be useful both to basic and clinical neuroscientists engaged in the field of neurology, neurophysiology, neurochemistry, neurosurgery, neuropathology, and related disciplines in order to understand basic functions of the spinal cord and to stimulate further research in this rapidly advancing field.

This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system.

This book is concernced with the process of cell death in human cells by programmed cell death or apoptosis, and the modulation of this process by drugs. This is a new field of study and this volume represents the first comprehensive treatment of this subject matter. Its focus is on human biology and medicine. Apoptosis is a very prevalent type of cell death with importance in human diseases such as cancer, Alzheimer's disease and autoimmune disorders. The book combines the expertise of leading scientists and writers from fields as diverse as neurosciences, immunology, pathology and cancer research. There is detailed analysis of the molecular and cellular aspects of apoptosis including the specific genes and molecules involved in the regulation of this special form of programmed cell death. Scientists, clinicians and researchers involved in studies of human biology and medicine will find this book an excellent resource.

Venous Ulcers, Second Edition, provides a comprehensive synthesis of evidence-based recommendations and the highest level of expertise from a leading group of doctors, which is a fundamental constituent for the appropriate management of nonhealing venous wounds in everyday practice. This book offers a fertile environment for a complete understanding of genetics and molecular and biochemical mechanisms that lead to the development and progression of venous ulcers, which is essential for elucidating the underlying pathophysiology and can be utilized for developing novel therapies and accessing previously inaccessible areas of research. Socioeconomic impact, impact on health-related quality of life, the clinical course of the disease, and diagnostic algorithms are elaborated in detail. All currently available treatment modalities are explained in a clinically applicable approach with particular emphasis on operative technique, technical feasibility, success rates (both clinical and technical), and side effects. Lastly, this book elaborates on special diagnostic considerations and management of the most complex patients, often requiring the highest level of expertise for successful treatment.

For students and clinical professionals who are learning anatomy, participating in a dissection lab, sharing anatomy knowledge with patients, or refreshing their anatomy knowledge, the Netter Atlas of Human Anatomy illustrates the body, region by region, in clear, brilliant detail from a clinician's perspective. Unique among anatomy atlases, it contains illustrations that emphasize anatomic relationships that are most important to the clinician in training and practice. Illustrated by clinicians, for clinicians, it contains more than 550 exquisite plates plus dozens of carefully selected radiologic images for common views. Presents world-renowned, superbly clear views of the human body from a clinical perspective, with paintings by Dr. Frank Netter as well as Dr. Carlos A. G. Machado, one of today's foremost medical illustrators. Content guided by expert anatomists and educators: R. Shane Tubbs, Paul E. Neumann, Jennifer K. Brueckner-Collins, Martha Johnson Gdowski, Virginia T. Lyons, Peter J. Ward, Todd M. Hoagland, Brion Benninger, and an international Advisory Board. Offers region-by-region coverage, including muscle table appendices at the end of each section and quick reference notes on structures with high clinical significance in common clinical scenarios. Contains new illustrations by Dr. Machado including clinically important or difficult to understand areas such as the Cavitas pelvis, Fossa temporalis and Fossa infratemporalis, Conchae nasi, and more. Features new nerve tables devoted to the Nervi craniales, Plexus cervicalis, Plexus brachialis, and Plexus lumbosacralis. Uses updated terminology based on the international anatomic standard, Terminologia Anatomica, with common clinical eponyms included. Enhanced eBook version included with purchase. Your enhanced eBook allows you to access all of the text, figures, and references from the book on a variety of devices. Provides access to extensive digital content: every plate in the Atlas?and over 100 bonus plates including illustrations from previous editions?is enhanced with an interactive label quiz option. Also available: . Netter Atlas of Human Anatomy: Classic Regional Approach -With US English terminology. . Netter Atlas of Human Anatomy: A Systems Approach-With US English terminology. Same content as the classic regional approach, but organized by body system. All options contain the same table material and 550+ illustrated plates painted by clinician artists, Frank H. Netter, MD, and Carlos Machado, MD.

Explore the science behind over 50 pilates exercises to master each movement with confidence and control. DK brings you a ground-breaking health book that will help you revitalise your workouts and perfect your Pilates practice, by understanding the muscle action, breathwork and techniques required, every step of the way. Did you know that performing Pilates can strengthen your core, improve flexibility and posture, and help to reduce stress and anxiety at the same time? Backed by scientific research, learn how Pilates exercises benefit every system in the body. Science of Pilates reveals the facts with annotated artworks that show the mechanics, the angles, how your blood flow and respiration are affected, the key muscle and joint actions working below the surface of each exercise, safe alignment, and much more! Go on a journey of self-discovery to explore: - Specially commissioned CGI artworks depict all the main Pilates exercises with variations that add or reduce challenge. - Artworks detail the mechanics of each exercise, correct body posture, the muscles involved and how they engage to perform movements. - Illustrations featuring colour-coding to highlight how the muscles, ligaments, and joints engage, stretch, and relax to perform each exercise. - Pilates programmes tailored to different abilities and aims. - Suggested practices with a progressive increase in challenge over weeks and months. - Easy-to-follow infographics help explain the hard science behind why Pilates training is so effective and what beneficial physical adaptations it can bring. Set out to unearth the facts behind the pseudo-science fads, The Science of Pilates is an approachable, entertaining and easy-to read fitness guide for those seeking self-development backed up by solid scientific evidence. The popular question and answer format brings an immediacy to the information provided, and the highly visually illustrations truly bring the science to life in a contemporary and accessible way. At DK, we believe in the power of discovery. So why stop there? If you like Science of Pilates, then why not try Science of Yoga to help you better understand yoga anatomy in order to perfect your practice and poses, or Science of Strength Training to support you in your journey to build you strongest body. Complete the collection today!

1) Classic anatomical atlases 2) Detailed labeling of the earliest phases of prenatal neorological development 3) Appeals to neuroanatomists, developmental biologists and clinical practioners. 4) Persistent relevance - brain development is not going to change.

This expanded edition includes a new introduction by John Nix as well as a new appendix offering case studies and practical insights from teachers about how Barbara Doscher has impacted their voice instruction. An understanding of how the singing voice functions should be a prerequisite for all those who wish to teach singing. And fundamental to any safe and efficient teaching method is the recognition that the singing voice is a functional unit. Consequently, this second edition, like the first, describes the anatomy and physiology of breathing and phonation and examines the acoustical laws necessary for an understanding of resonation. It is designed primarily as a textbook for college vocal pedagogy courses. The book includes detailed illustrations and an expansion of the appendix on vocal misuse and abuse, including data on subglottic air pressure/air flow ratios, the phonatory mode known as belting, and the aging voice, especially as it is affected by hormonal changes in the body. It also feature an extensive bibliography.

This edited book explores the use of technology to enable us to visualise the life sciences in a more meaningful and engaging way. It will enable those interested in visualisation techniques to gain a better understanding of the applications that can be used in visualisation, imaging and analysis, education, engagement and training. The reader will also be able to learn about the use of visualisation techniques and technologies for the historical and forensic settings. The chapters presented in this volume cover such a diverse range of topics, with something for everyone. We present here chapters on 3D visualising novel stent grafts to aid treatment of aortic aneuryms; confocal microscopy constructed vascular models in patient education; 3D patient specific virtual reconstructions in surgery; virtual reality in upper limb rehabilitation in patients with multiple sclerosis and virtual clinical wards. In addition, we present chapters in artificial intelligence in ultrasound guided regional anaesthesia; carpal tunnel release visualisation techniques; visualising for embryology education and artificial intelligence data on bone mechanics. Finally we conclude with chapters on visualising patient communication in a general practice setting; digital facial depictions of people from the past; instructor made cadaveric videos, novel cadaveric techniques for enhancing visualisation of the human body and finally interactive educational videos and screencasts. This book explores the use of technologies from a range of fields to provide engaging and meaningful visual representations of the biomedical sciences. It is therefore an interesting read for researchers, developers and educators who want to learn how visualisation techniques can be used successfully for a variety of purposes, such as educating students or training staff, interacting with patients and biomedical procedures in general.

Netter's Integrated Musculoskeletal System is an innovative new text that brings together basic science material from several domains, providing a solid foundation prior to delving into topics of increasing complexity and clinical importance -all highlighted by superb Netter illustrations throughout. Initial chapters give a general overview of the human body, while the remaining chapters examine all facets of the musculoskeletal system, the injuries that affect it at the macroscopic and microscopic levels, and the process of development. As the scientific content becomes more complex, the clinical correlations become more specific. This progressively constructed narrative guides readers efficiently and effectively through the intricacies of the musculoskeletal system in a way that is easy to understand and remember-all in a single, time-saving resource for busy students. Takes an integrated approach including gross anatomy, physiology, biochemistry, neuroscience, histology, and other relevant sciences to better help readers understand the musculoskeletal system. Presents essential content in an easy-to-understand manner, puts it in context, and then elaborates on it with more detail-making connections between content areas and reducing the need for multiple study resources. Features clinical correlations boxes throughout; includes an appendix of commonly-used eponyms to help readers communicate across disciplines and an appendix of Latin/Greek/Arabic roots for anatomical terms. Designed to be used effectively in longitudinally-designed, integrated curricula-for a wide range of health-science students-with carefully organized, concise reading assignments and discrete areas of study for each lesson. Enhanced eBook version included with purchase. Your enhanced eBook allows you to access all of the text, figures, and references from the book on a variety of devices.

The Encyclopedia of Cell Biology, Four Volume Set offers a broad overview of cell biology, offering reputable, foundational content for researchers and students across the biological and medical sciences. This important work includes 285 articles from domain experts covering every aspect of cell biology, with fully annotated figures, abundant illustrations, videos, and references for further reading. Each entry is built with a layered approach to the content, providing basic information for those new to the area and more detailed material for the more experienced researcher. With authored contributions by experts in the field, the Encyclopedia of Cell Biology provides a fully cross-referenced, one-stop resource for students, researchers, and teaching faculty across the biological and medical sciences.

This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.

A cutting-edge review of the biochemical, physiological, pharmacological, genetic, and molecular interactions involved in the development and homeostasis of the skeleton. Topics range from chondrogenesis, chondrocytes, and cartilage to skeletal dysmorphology, and include the control of skeletal development, osteoblastic cell differentiation, and bone induction, growth, remodeling, and mineralization. The authors' understanding of bone physiology-and how it is modified throughout all the stages of life-offers novel approaches for improving the endurance of load-bearing implants, achieving life-long optimal bone strength, overcoming microgravity situations (space flight), and hastening the healing of fractures, osteotomies, and antrodeses.

The Anatomical Male Muscular System, Second Edition is a modern update of the bestselling, classic The Muscular System chart dating back to 1947. The illustrations are vividly colored and finely detailed, and structures are clearly labeled. The two large central illustrations show anterior and posterior views of the anatomical male muscular system, and four smaller inset illustrations show: Right Half of Diaphragm Muscles of Right Hand (Palmar View) Muscles of Posterior Abdominal Wall Muscles of the Right Foot (Plantar View) 20" x 26" heavy paper laminated with grommets at top corners