This is the fifth time a "Horticultural Research International" has been published, again revised and extended. Since 1986 a lot has changed in the world: new countries with old names appeared on the map, while recognized frontiers vanished. As the organizing editors we tried to follow these evolutions as closely as possible. In many cases we were able to establish communication with our correspondents as usual; nevertheless we had to delete a total of 8 countries. Thanks to correspondents from 74 different countries we are now able to present this edition, comprising a total of 1800 institutes, including 20,000 names of individual research workers. We are very grateful to the correspondents from 17 newly enlisted countries who performed pioneer work for us. Noteworthy is the fact that especially the Asian continent made a leap forward with contributions from 13 countries in 1986 to 19 in the present volume of "Horticultural Research International". At the secretariat we will continue to extend our network, so please feel free to send us any suggestions or additions you would like to make. For the first time we added a list of international organizations relevant to horticulture. In the editing process a further standardization has been used to reach an easily readable and comparable survey per country. Hopefully this directory will again facilitate cooperation on a worldwide scale among horticulturists active in governmental, academic, extensional, educational, technical or commercial fields.

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Presents new ideas on classification of proteolytic enzymes
* Covers 100 individual proteolytic enzymes
* Offers numerous medical implications of work in this area
* Provides uses of these enzymes in biotechnology

A prime reference volume for geneticists, food technologists and biotechnologists in the academic and industrial sectors. Fermentations with lactic acid bacteria determine important qualities such as taste, shelf-life, and food values. New methods of food production require fast and reliable manufacture, which has led to a dramatic surge of interest in the genetic, microbiological and biochemical properties of lactic acid bacteria.

This book was written with the purpose of providing a sound basis for the design of enzymatic reactions based on kinetic principles, but also to give an updated vision of the potentials and limitations of biocatalysis, especially with respect to recent app- cations in processes of organic synthesis. The ?rst ?ve chapters are structured in the form of a textbook, going from the basic principles of enzyme structure and fu- tion to reactor design for homogeneous systems with soluble enzymes and hete- geneous systems with immobilized enzymes. The last chapter of the book is divided into six sections that represent illustrative case studies of biocatalytic processes of industrial relevance or potential, written by experts in the respective ?elds. We sincerely hope that this book will represent an element in the toolbox of gr- uate students in applied biology and chemical and biochemical engineering and also of undergraduate students with formal training in organic chemistry, biochemistry, thermodynamics and chemical reaction kinetics. Beyond that, the book pretends also to illustrate the potential of biocatalytic processes with case studies in the ?eld of organic synthesis, which we hope will be of interest for the academia and prof- sionals involved in R&D&I. If some of our young readers are encouraged to engage or persevere in their work in biocatalysis this will certainly be our more precious reward.

We have known about the existence of killer lymphocytes since 1960, when they were discovered in connection with transplant rejection in vivo. Since then we have uncovered at least five subsets of lymphocytes that can kill other cells in vitro, establishing the study of cell-mediated cytotoxicity (CMC) as a major field of immunological inquiry. Berke and Clark summarize the extensive literature based on the study of CMC in vitro. Several important questions about killer cells have now been answered, for example, how they go about destroying other cells. Research ultimately revealed at least three lytic mechanisms available to killer lymphocytes. But do killer cells actually use these mechanisms in vivo? The possible involvement of CMC in transplant rejection, control of intracellular parasites, cancer, autoimmunity, and immune homeostatic regulation is analyzed in detail, yielding some surprising findings, and outlining important questions that remain unanswered.

This extensively documented, comprehensive survey of cell-mediated cytotoxicity traces the history of killer lymphocytes from 1960 to the present, providing a definitive resource for specialists and non-specialists alike.

Carbohydrate Chemistry provides review coverage of all publications relevant to the chemistry of monosaccharides and oligosaccharides in a given year. The amount of research in this field appearing in the organic chemical literature is increasing because of the enhanced importance of the subject, especially in areas of medicinal chemistry and biology. In no part of the field is this more apparent than in the synthesis of oligosaccharides required by scientists working in glycobiology. Clycomedicinal chemistry and its reliance on carbohydrate synthesis is now very well established, for example, by the preparation of specific carbohydrate- based antigens, especially cancer-specific oligosaccharides and glycoconjugates. Coverage of topics such as nucleosides, amino-sugars, alditols and cyclitols also covers much research of relevance to biological and medicinal chemistry. Each volume of the series brings together references to all published work in given areas of the subject and serves as a comprehensive database for the active research chemist Specialist Periodical Reports provide systematic and detailed review coverage in major areas of chemical research. Compiled by teams of leading authorities in the relevant subject areas, the series creates a unique service for the active research chemist, with regular, in-depth accounts of progress in particular fields of chemistry. Subject coverage within different volumes of a given title is similar and publication is on an annual or biennial basis.

The Office of Health and Environmental Research (OHER) has supported and continues to support development of computational approaches in biology and medicine. OHER's Radiological and Chemical Physics Program initiated development of computational approaches to determine the effects produced by radiation of different quality (such as high energy electrons, protons, helium and other heavy ions, etc. ) in a variety of materials of biological interest-such as water, polymers and DNA; these include molecular excitations and sub-excitations and the production of ionization and their spatial and temporal distribution. In the past several years, significant advances have been made in computational methods for this purpose. In particular, codes based on Monte Carlo techniques have .been developed that provide a realistic description of track-structure produced by charged particles. In addition, the codes have become sufficiently sophisticated so that it is now possible to calculate the spatial and temporal distribution of energy deposition patterns in small volumes of subnanometer and nanometer dimensions. These dimensions or resolution levels are relevant for our understanding of mechanisms at the molecular level by which radiations affect biological systems. Since the Monte Carlo track structure codes for use in radiation chemistry and radiation biology are still in the developmental stage, a number of investigators have been exploring different strategies for improving these codes."

The fascinating machinery that life uses to harness energy is the focus of this volume of the Advances in Photosynthesis and Respiration series. Experts in the field communicate their insights into the mechanisms that govern biological energy conversion from the atomic scale to the physiological integration within organisms. By leveraging the power of current structural techniques the authors reveal the inner workings of life.

Intended for researchers in biochemistry, medicine, nutrition and industry, this book discusses such topics as components of bacterial polysaccharides, fluorinated carbohydrates, and carbon sugars chemistry.

Immunofluorescence, a suitable laboratory method for the microscopic demonstration of antigens and antibodies in biological materials, useable, for example, to provide evidence for the pathogenesis of disease in histological or cytological preparations and for tumour cell differentiation. For this reason immunofluorescence has a decisive role as the method of choice for the diagnosis of auto-immune diseases. This primer on immunofluorescence techniques, which first appeared in 1979, is a richly illustrated handbook suitable for everyday practical work in the laboratory, useable as both an introduction to the subject as well as an atlas. In hardly any other area of medicine are there so many new findings to report. The second edition of this book is concerned not only with the detection methods which now form an essential and established part of diagnostic techniques, but also with the most recent research results such as the discovery of antibodies against Auerbach's plexus and against podocytes...

Reviews of Environmental Contamination and Toxicology provides concise, critical review articles of timely advances, philosophy and significant areas of accomplished or needed endeavor in the total field of xenobiotics, in any segment of the environment, as well as toxicological implications.

While systems biology and pharmacodynamics have evolved in parallel, there are significant interrelationships that can enhance drug discovery and enable optimized therapy for each patient. Systems pharmacology is the relatively new discipline that is the interface between these two methods. This book is the first to cover the expertise from systems biology and pharmacodynamics researchers, describing how systems pharmacology may be developed and refined further to show practical applications in drug development. There is a growing awareness that pharmaceutical companies should reduce the high attrition in the pipeline due to insufficient efficacy or toxicity found in proof-of-concept and/or Phase II studies. Systems Pharmacology and Pharmacodynamics discusses the framework for integrating information obtained from understanding physiological/pathological pathways (normal body function system vs. perturbed system due to disease) and pharmacological targets in order to predict clinical efficacy and adverse events through iterations between mathematical modeling and experimentation.

For courses in General, Organic, and Biological Chemistry (2 - Semester) This package includes MasteringChemistry (TM) A Clear, Flexible Approach to Chemistry for the Modern Classroom Active learning, an increased focus on clinical examples, updates based on current teaching and research findings, and digital innovations designed to engage and personalize students' experience make Fundamentals of General, Organic, and Biological Chemistry simply the best choice for students with a future in allied health. With the Eighth Edition, the authors make learning chemistry a more active experience through features designed to get students doing chemistry. Every chapter features Hands on Chemistry sections that deepen students' understanding of chemistry by having them perform elementary experiments with everyday household items. Group Problemsat the end of every chapter are designed for in-class use and motivate students toward higher level thinking, such as how concepts fit together and how to apply these concepts in a clinical application. All of the chapter openers, including many of the Chemistry in Action boxes and end-of-chapter problems, have been rewritten for a stronger clinical focus that provides more relevance to allied health majors. All content has been updated for the modern classroom with special attention to the biochemistry chapters, making the Eighth Edition of Fundamentals of General, Organic and Biological Chemistry the best choice for future allied health students. This edition is fully integrated with MasteringChemistry to provide an interactive and engaging experience. Media resources include narrated Video Tutor Solutions for every book chapter that present how to work the most challenging problems and feature additional feedback and instruction from contributor Sara Madsen. NEW in MasteringChemistry is the Chemistry Primer, a diagnostic and remediation tool that provides a pre-built assignment designed to get students up to speed on Chemistry and Math skills at the beginning of the course so they come to class prepared to delve more deeply into topics. This package includes MasteringChemistry, the leading online homework, tutorial, and assessment system, designed to improve results by engaging students before, during, and after class with powerful content. Instructors ensure students arrive ready to learn by assigning educationally effective content before class, and they encourage critical thinking and retention with in-class resources such as Learning Catalytics (TM). MasteringChemistry should only be purchased when required by an instructor. Please be sure you have the correct ISBN and Course ID. Instructors, contact your Pearson representative for more information.

Flavonoids are secondary plant products that have previously been shown to be helpful in determining relationships among plant groups. This work presents comprehensively the occurrence, patterns of variation, and systematic and evolutionary importance of flavonoids in the sunflower family (Asteraceae), the largest family of flowering plants (23,000 species). It gathers together the more than 2500 reports of flavonoids in Asteraceae published between 1950 to the present and interprets these data in context of new taxonomic (especially generic) alignments. The authors discuss flavonoid patterns with reference to modern phylogenetic studies based on morphology and DNA data. This book provides, therefore, the most exhaustive synthesis and evaluation of the systematic and evolutionary import of flavonoids ever accomplished for any large family of angiosperms.

Quantum Simulations of Materials and Biological Systems features contributions from leading world experts in the fields of density functional theory (DFT) and its applications to material and biological systems. The recent developments of correlation functionals, implementations of Time-dependent algorithm into DFTB+ method are presented. The applications of DFT method to large materials and biological systems such as understanding of optical and electronic properties of nanoparticles, X-ray structure refinement of proteins, the catalytic process of enzymes and photochemistry of phytochromes are detailed. In addition, the book reviews the recent developments of methods for protein design and engineering, as well as ligand-based drug design. Some insightful information about the 2011 International Symposium on Computational Sciences is also provided. Quantum Simulations of Materials and Biological Systems is aimed at faculties and researchers in the fields of computational physics, chemistry and biology, as well as at the biotech and pharmaceutical industries.

Complex and unexplained phenomena tend to foster unorthodox perspectives. This publication is an example, as is a prior publication that emphasized the concept that intermediary metabolism might play a significant and determining role in hepatocyte proliferation and 1 tumorigenesis. Formulation of this hypothesis was based on an attempt to clarify several poorly understood phenomena; including the observations: 1) that xenobiotic peroxisome proliferators such as the fibrate hypolipidemic agents induce hepatocyte proliferation and carcinogenesis in rodents; 2) that benign and malignant liver tumors complicate the human syndrome of glycogen storage disease type I (glucose-6-phosphatase deficiency); and 3) that in this same syndrome, administration of glucose exerts an anti-tumor effect. Fatty acid and glucose metabolism are tightly linked in a we- established and profoundly inportant interplay. This connection, together with the fact that peroxisome proliferator-induced hepatocyte proliferation and carcinogenesis reflects inhibition of mitochondrial carnitine palmitoyltransferase-I and fatty acid oxidation, suggested the possibility that regulation of fatty acid metabolism could prove to be a pivotal determinant in the control of cell growth. In 1993, the year in which the paper cited above was published, insight into the importance of growth factors and signal transduction pathways in cell cycle regulation was increasing rapidly, but metabolic and energetic aspects of cell proliferation had attracted relatively little attention. Despite this, the concept seemed inescapable that the two seemingly distinct and unrelated determinants - signal transduction and metabolism - were integrally linked.

This volume contains comprehensive reviews on the current status of knowledge concerning the physiological, biochemical, and molecular events underlying plant morphogenesis processes authored by the main lecturers at the NATO Advanced Study Institute which was held in Heraklion, Crete, Greece, Septe

The discovery of microRNAs and its role as gene expression regulators in human carcinogenesis represents one of the most important scientific achievements of the last decade. More recently, other non-coding RNAs have been discovered and its implications in cancer are emerging as well, suggesting a broader than anticipated involvement of the non-coding genome in cancer. Moreover, completely new and unexpected functions for microRNAs are being revealed, leading to the identification of new anticancer molecular targets. This book represents a comprehensive guide on non-coding RNAs and cancer, spanning from its role as cancer biomarkers, to providing the most useful bioinformatic tools, to presenting some of the most relevant discoveries, which indicates how these fascinating molecules act as fine orchestrators of cancer biology.

This book offers a comprehensive study of biological molecules acquired from marine organisms, which have been exploited for drug discovery with the aim to treat human diseases. Biomolecules have potential impacts on a diverse range of fields, including medical and pharmaceutical science, industrial science, biotechnology, basic research, molecular science, environmental science and climate change, etc. To understand and effectively apply medicinally important biomolecules, multidisciplinary approaches are called for. The ocean remains a rich biological resource, and the vast untapped potential of novel molecules from marine bio-resources has caught the interest of more and more researchers. These novel biological compounds have never been found in terrestrial or other ecosystems, but only in this rich niche. Advances in sampling techniques and technologies, along with increased funding for research and nature conservation, have now encouraged scientists to look deeper in the waters. Aquaculture supports both tremendous seafood production and the bulk production of marine-derived drugs. Furthermore, molecular methods are now being extensively employed to explore the untapped marine microbial diversity. With the help of molecular and biotech tools, the ability of marine organisms to produce new biosynthetic drugs can be greatly enhanced. This book provides an extensive compilation of the latest information on marine resources and their undisputedly vital role in the treatment of diverse ailments.

Ion channels are the major class of membrane proteins responsible for rapid and regulated transport of ions across biological membranes and for the generation and propagation of electrical signals in the brain, heart, and skeletal and vascular tissues. Ion channels are also known to play critical roles in regulation of cell proliferation, insulin secretion and intracellular signaling in a variety of cell types. This book focuses on the roles of ion channels in vascular tissues under normal and pathological conditions. Vascular abnormalities are known to underlie a plethora of severe pathological conditions, such as atherosclerosis, systemic and pulmonary hypertension, coronary or cerebral vasospasm, and diabetes. In addition, misregulated angiogenesis is one of the major contributors to the development of tumors. Therefore, it is clearly imperative to obtain a better understanding of the molecular mechanisms that contribute to vascular disorders. This book will be the first comprehensive assembly of assays to present the studies that have been done during the last decade to elucidate the roles of ion channels in different vascular diseases.

Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry.

Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology. In this second edition of "Bioconjugation Protocols: Strategies and Methods," expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering. These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches. Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices). All the protocols follow the successful "Methods in Molecular Biology"TM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and highly practical, "Bioconjugation Protocols: Strategies and Methods, Second Edition" offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials."

The International Society on Oxygen Transport to Tissue (lSOTT) held its 26th annual meeting from August 23-26, 1998, and met for the second time in Budapest. As captured in the design of the ISOTT'98 logo, the venue of the conference was the Budapest Hilton in the heart of the historic Castle District in Buda, across from Hungary's coronation church-the Matthias Church-and the fairy-tale-like Fisherman's Bastion; a special place with a historical touch situated atop the Castle Hill, from where participants enjoyed the spectacular panoramic views of Budapest exquisitely laid out on both sides of the Danube. In preparation for ISOTT'98, major emphasis was given to the application of informatics in collecting, presenting, and disseminating scientific and other information associated with the meeting. Electronic submission of the abstracts made it possible to publish the illustrated Abstracts on the ISOTT'98 Web Site well before the meeting. Following the meeting, an ISOTT'98 CD was published as a digital, searchable record of ISOTT'98. The scientific program was designed with the active participation of prospective attendees, in that priority and momentum was given to the topics selected by those visiting the Web Site. Poster and oral presentations were considered as equivalently effective formats, reflected by the fact that posters were on display throughout the meeting.

This book discusses UV radiation, its effects on ecosystems and the likely evolutionary consequences of changed UV radiation environments, past, present and future. The first two chapters examine the history of the UV radiation climate of earth and the factors that determine organismal and ecosystem exposure. Their purpose is to give the reader a physical perspective on UV radiation and an understanding of the constantly changing UV environment that ecosystems are exposed to over time. Variations in the UV radiation environment occur at the local level (such as boundary layer and plant canopy effects) through to global-scale changes (such as alterations in the column abundance of UV-B protecting ozone). UV radiation regimes also vary over temporal scales. These alterations occur on time scales of seconds (the movement of clouds and plant canopies) to literally billions of years (gross long-term changes in the composition of the Earth's atmosphere). In the chapters that follow five specific biological and ecological topics in photobiology are considered. They are effects of UV radiation on amphibians, plants, corals, aquatic microbial ecosystems and Antarctic ecosystems that are exposed to the anthropogenically generated ozone 'hole'. These chapters consider UV radiation effects at a diversity of levels from the biochemical to the community. Their purpose is to provide the reader with our current understanding of the ecological effects of UV radiation, the areas where questions still remain and to provide a perspective from which the reader can better understand questions in evolutionary photobiology. The final chapter investigates the biological consequences of altered extraterrestrial ultraviolet fluxes, which are quite different from those experienced on the Earth. Our knowledge of the role of UV radiation in shaping ecologies and evolutionary change is still in its infancy. This book brings together a number of authors with the aim of helping to consolidate a better understanding of this interesting area of photobiology.