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Books > Science & Mathematics > Biology, life sciences > Life sciences: general issues > Genetics (non-medical) > DNA
"Advances in Botanical Research" publishes in-depth and up-to-date
reviews on a wide range of topics in plant sciences.
This volume of Methods in Enzymology aims to provide a reference
for the diverse, powerful tools used to analyze RNA helicases.
Thecontributions inthis volume cover the broad scope of methods in
the research on these enzymes. Several chapters describe
quantitative biophysical and biochemical approaches to study
molecular mechanisms and conformational changes of RNA helicases.
Further chapters cover structural analysis, examination of
co-factor effects on several representative examples, and the
analysis of cellular functions of select enzymes. Two chapters
outline approaches to the analysis of inhibitors that target RNA
helicases.
Metagenomics has taken off as one of the major cutting-edge fields of research. The field has broad implications for human health and disease, animal production and environmental health. Metagenomics has opened up a wealth of data, tools, technologies and applications that allow us to access the majority of organisms that we still cannot access in pure culture (an estimated 99% of microbial life). Numerous research groups are developing tools, approaches and applications to deal with this new field, as larger data sets from environments including the human body, the oceans and soils are being generated. See for example the human microbiome initiative (HMP) which has become a world-wide effort and the Global Ocean Sampling (GOS) surveys. The number of publications as measured through PubMed that are focused on metagenomics continues to increase. The field of metagenomics continues to evolve with large common datasets available to the scientific community. A concerted effort is needed to collate all this information in a centralized place. By having all the information in an Encyclopedia form, we have an opportunity to gather seminal contributions from the leaders in the field, and at the same time provide this information to a significant number of junior and senior scientists. It is anticipated that the Encyclopedia will also be used by many other groups including, clinicians, undergraduate and graduate level students, as well as ethical and legal groups associated with or interested in the issues surrounding metagenome science.
This new volume in the "Current topics in Developmental Biology"
series concentrates on MicroRNAs in Development. It includes
chapters on such topics as miRNA networks in neuronal development,
let-7 in development, and Hox networks and miRNA. With an
international team of authors, this volume is a must-have addition
for researchers and students alike.
This book describes how genomics has revolutionized our scientific understanding of agriculturally important plant-associated bacteria. Each chapter focuses on the genomics of particular bacteria: the first described plant pathogen, "Erwinia amylovora"; phytoplasmas lacking cell walls; fastidious, phloem-restricted liberibacters; "Pseudomonas syringae," which is a genetically tractable model system; "Xanthomonas citri," which causes a disease that can devastate citrus crops and "Pseudomonas fluorescens," which can protect plants from diseases. Topics considered in this volume include the importance of horizontal gene transfer in originating new bacterial strains and species and advances in transcriptomics that allow us to describe the complex regulatory networks critical to plant-microbe interactions. The availability of the "Xanthomonas oryzae" genome has led to new technologies in genome editing, which will revolutionize approaches to genetic engineering, even in eukaryotes. The contributions show how genomics has greatly accelerated progress toward understanding the biology of these bacteria and how that understanding can be translated into novel crop protection methods.
Metagenomics has taken off as one of the major cutting-edge fields of research. The field has broad implications for human health and disease, animal production and environmental health. Metagenomics has opened up a wealth of data, tools, technologies and applications that allow us to access the majority of organisms that we still cannot access in pure culture (an estimated 99% of microbial life). Numerous research groups are developing tools, approaches and applications to deal with this new field, as larger data sets from environments including the human body, the oceans and soils are being generated. See for example the human microbiome initiative (HMP) which has become a world-wide effort and the Global Ocean Sampling (GOS) surveys. The number of publications as measured through PubMed that are focused on metagenomics continues to increase. The field of metagenomics continues to evolve with large common datasets available to the scientific community. A concerted effort is needed to collate all this information in a centralized place. By having all the information in an Encyclopedia form, we have an opportunity to receive seminal contributions from the leaders in the field and at the same time provide this information to a significant number of junior and senior scientists, via colleges, libraries, and just through online access. This format also allows scientists in the developing world to have continued access to this growing field. It is anticipated that the Encyclopedia will also be used by many other groups including, clinicians, undergraduate and graduate level students, as well as ethical and legal groups associated with or interested in the issues surrounding metagenome science.
The series Topics in Current Chemistry presents critical reviews of the present and future trends in modern chemical research. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. Review articles for the individual volumes are invited by the volume editors. Readership: research chemists at universities or in industry, graduate students.
DNA methylation is essential for the normal development and
functioning of organisms. This volume discusses the latest
developments in this very active field of research. It presents the
evolution of DNA methylation, mammalian DNA methyltransferases, DNA
methylation and demethlyation, DNA methylation and silencing and
the role it plays in medicine including cancer. * Discusses new discoveries, approaches, and ideas * Contributions from leading scholars and industry experts * Reference guide for researchers involved in molecular biology and related fields
This book contains articles written by experts on a wide range of topics that are associated with the analysis and management of biological information at the molecular level. It contains chapters on RNA and protein structure analysis, DNA computing, sequence mapping, genome comparison, gene expression data mining, metabolic network modeling, and phyloinformatics. The important work of some representative researchers in bioinformatics is brought together for the first time in one volume. The topic is treated in depth and is related to, where applicable, other emerging technologies such as data mining and visualization. The goal of the book is to introduce readers to the principle techniques of bioinformatics in the hope that they will build on them to make new discoveries of their own. Contents: Exploring RNA Intermediate Conformations with the Massively Parallel Genetic Algorithm; Introduction to Self-Assembling DNA Nanostructures for Computation and Nanofabrication; Mapping Sequence to Rice FPC; Graph Theoretic Sequence Clustering Algorithms and their Applications to Genome Comparison; The Protein Information Resource for Functional Genomics and Proteomics; High-Grade Ore for Data Mining in 3D Structures; Protein Classification: A Geometric Hashing Approach; Interrelated Clustering: An Approach for Gene Expression Data Analysis; Creating Metabolic Network Models Using Text Mining and Expert Knowledge; Phyloinformatics and Tree Networks. Readership: Molecular biologists who rely on computers and mathematical scientists with interests in biology.
The discovery of stress-induced mutagenesis has changed ideas about mutation and evolution, and revealed mutagenic programs that differ from standard spontaneous mutagenesis in rapidly proliferating cells. The stress-induced mutations occur during growth-limiting stress, and can include adaptive mutations that allow growth in the otherwise growth-limiting environment. The stress responses increase mutagenesis specifically when cells are maladapted to their environments, i.e. are stressed, potentially accelerating evolution then. The mutation mechanism also includes temporary suspension of post-synthesis mismatch repair, resembling mutagenesis characteristic of some cancers. Stress-induced mutation mechanisms may provide important models for genome instability underlying some cancers and genetic diseases, resistance to chemotherapeutic and antibiotic drugs, pathogenicity of microbes, and many other important evolutionary processes. This book covers pathways of stress-induced mutagenesis in all systems. The principle focus is mammalian systems, but much of what is known of these pathways comes from non-mammalian systems.
In recent years new discoveries have made this an exciting and
important field of research. This comprehensive volume presents
comprehensive chapters and detailed background information for
researchers working with in the field of Nuclear Mechanics and
Genome Regulation. * Both classic and state-of-the-art methods readily adaptable and designed to last the test of time * Relevant to clinicians and scientists working in a wide range of fields
This book summarizes the latest information and the status quo of radish genome studies to stimulate innovations and improvements in breeding techniques and to promote further advances in the field. Radish (Raphanus sativus) is a member of the Brassicaceae family and is cultivated worldwide. Its varieties have been diversified in terms of size, shape, and the color of their roots and bio-components. Thanks to the development of high-throughput molecular techniques using next generation sequencers, complete genomes of cultivated and wild radish plants have been sequenced and published with annotations of predicted genes and single nucleotide polymorphism (SNP) information between radish cultivars and accessions. These, together with the construction of a high-density genetic map of radish and profiling of expression sequences in radish organs, have accelerated genetic studies, such as the identification of genes or loci associated with root development, pungent components, and plant disease resistance. Providing an overview of these advances, this book is a valuable resource for scientists involved in plant genetic research and crop breeding.
Structural genomics is the systematic determination of
3-dimensional structures of proteins representative of the range of
protein structure and function found in nature. The goal is to
build a body of structural information that will predict the
structure and potential function for almost any protein from
knowledge of its coding sequence. This is essential information for
understanding the functioning of the human proteome, the ensemble
of tens of thousands of proteins specified by the human genome.
Appropriate for a wide range of disciplines, from biology to
non-biology, law and nursing majors, "DNA and Biotechnology" uses a
straightforward and comprehensive writing style that gives the
educated layperson a survey of DNA by presenting a brief history of
genetics, a clear outline of techniques that are in use, and
highlights of breakthroughs in hot topic scientific
discoveries. Engaging and straightforward scientific writing style Comprehensive forensics chapter Parallel Pedagogic material designed to help both readers and teachers. Highlights in the latest scientific discoveries Outstanding full-color illustration that walk reader through complex concepts
This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.
Burkholderia are a multi-faceted group of bacteria with considerable genetic and metabolic diversity and very versatile lifestyles. In this book leading international investigators review key advances in Burkholderia research to provide timely overview. The topics covered include: genomic taxonomy and biodiversity, comparative genomics, molecular epidemiology, transcriptomics, proteomics, molecular pathogenesis of virulence in B. mallei/B. pseudomallei and the Burkholderia cepacia complex. The theme underpinning each chapter is the use of DNA/protein sequence data and post-genomic technologies to understand Burkholderia biology.
* Comprehensive and an easily accessible reference volume for developing, running, and analyzing biomedical research using the rat as model system Grown exponentially by the genomic revolution, the use of the rat as a model of choice for physiological studies continues in popularity and at a much greater depth of understanding. In Rat Genomics: Methods and Protocols, world-wide experts provide both practical information for researchers involved in genomic research in the rat along with a more contextual discussion about the usefulness of the rat in physiological or translational research in different organs and systems. The volume extensively covers topics including genome sequencing, quantitative trait loci mapping, and the identification of single nucleotide polymorphisms as well as the development of transgenic technologies such as nuclear cloning, lentiviral-mediated transgenesis, gene knock-down using RNA interference, gene knock-out by mutagenesis, and zinc finger nucleases plus exciting advances in the obtention of rat embryonic cell lines. As a volume in the highly successful Methods in Molecular BiologyT series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results. Comprehensive and up-to-date, Rat Genomics: Methods and Protocols thoroughly covers the current techniques used in labs around the world and overviews the applications of the data obtained, making it certain to be useful to the scientific community as a key source of references and methods.
Specific complexes of protein and RNA carry out many essential
biological functions, including RNA processing, RNA turnover, and
RNA folding, as well as the translation of genetic information from
mRNA into protein sequences. Messenger RNA (mRNA) decay is now
emerging as an important control point and a major contributor to
gene expression. Continuing identification of the protein factors
and cofactors and mRNA instability elements responsible for mRNA
decay allow researchers to build a comprehensive picture of the
highly orchestrated processes involved in mRNA decay and its
regulation.
After the generation of genome sequence data from a wide variety of plants, databases are filled with sequence information of genes with no known biological function, and while bioinformatics tools can help analyze genome sequences and predict gene structures, experimental approaches to discover gene functions need to be widely implemented. In Plant Reverse Genetics: Methods and Protocols, leading researchers in the field describe cutting-edge methods, both high-throughput and genome-wide, involving the models Arabidopsis and rice as well as several other plants to provide comparative functional genomics information. With chapters on the analysis of high-throughput genome sequence data, the identification of non-coding RNA from sequence information, the comprehensive analysis of gene expression by microarrays, and metabolomic analysis, the thorough methods of the book are fully supported by scripts to aid their computational use. Written in the highly successful Methods in Molecular Biology (TM) series format, the chapters contain introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Plant Reverse Genetics: Methods and Protocols is an ideal guide for researchers seeking an understanding of how the complex web of plant genes work together in a systems biology view.
This text explores the most recent advances in NGS instrumentation and data anlysis. It begins with a comprehensive description of current NSG platforms, their sequencing chemistries, instrument specifications, and general workflows and procedures. A separate chapter is dedicated to low-quanitity, single molecule sequencing technology. Further chapters explore the application of NSG technologies in various fields.
Transposable elements have played a major role in shaping plant genome structure and gene expression. Transposons not only drive sequence expansion, induce mutations and generate chromosome rearrangements, they also help to shape the epigenetic topology of the eukaryotic genome. In Plant Transposable Elements: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study transposons. These methods include computational approaches to study the ancient transposon remnants that comprise the bulk of plant genomes, as well as laboratory techniques to identify recent and ongoing transposition events. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Transposable Elements: Methods and Protocols seeks to aid scientists in the further study of transposons by providing essential background information and specific experimental protocols.
Charge migration through DNA has been the focus of considerable interest in recent years. This book presents contributions from an international team of researchers active in this field. It contains a wide range of topics that includes the mathematical background of the quantum processes involved, the role of charge transfer in DNA radiation damage, a new approach to DNA sequencing, DNA photonics, and many others.
In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination. With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.
Since the independent invention of DNA sequencing by Sanger and by
Gilbert 30 years ago, it has grown from a small scale technique
capable of reading several kilobase-pair of sequence per day into
today's multibillion dollar industry. This growth has spurred the
development of new sequencing technologies that do not involve
either electrophoresis or Sanger sequencing chemistries. Sequencing
by Synthesis (SBS) involves multiple parallel micro-sequencing
addition events occurring on a surface, where data from each round
is detected by imaging.
DNA Repair and Human Disease highlights the molecular complexities of a few well-known human hereditary disorders that arise due to perturbations in the fidelity of diverse DNA repair machineries. |
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