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Books > Medicine > Pre-clinical medicine: basic sciences > Anatomy > General
Ribonucleic acid (RNA) binding proteins currently number in the
thousands and defects in their function are at the heart of
diseases such as cancer and neurodegeneration. RNA binding proteins
have become implicated in the intricate control of surprisingly
diverse biological settings, such as circadian rhythm, stem cell
self-renewal, oncogenesis and germ cell development. This book
surveys a range of genome-wide and systems approaches to studying
RNA binding proteins, the importance of RNA binding proteins in
development, cancer and circadian rhythm.
The Micro-Tomographic Atlas of the Mouse Skeleton provides a unique
systematic description of all calcified components of the mouse. It
includes about 200 high resolution, two and three dimensional m CT
images of the exterior and interiors of all bones and joints. In
addition, the spatial relationship of bones within complex skeletal
units is also described. The images are accompanied by detailed
explanatory text, thus highlighting special features and newly
reported structures. The Atlas fulfils an emerging need for a
comprehensive reference to assist both trained and in-training
researchers.
This volume focuses on the cell biology and physiology of skeletal
muscle regeneration. This Book is a collection of classic and
cutting edge protocols optimized for mice, but in most cases
adaptable to rat or other mammalian models, that will allow an
investigator to develop and implement a research study on skeletal
muscle regeneration. Chapters address the three major areas of
study: provoking regeneration by inducing damage to muscle,
analyzing the progenitor cells of skeletal muscle, and quantifying
overall muscle function. Subjects discussed include: inducing
skeletal muscle injury by eccentric contraction; volumetric muscle
loss; single myofiber isolation and culture; satellite cell
transplantation; muscle clearing for whole mount immunostaining;
luciferase tracking of muscle stem cells; mitochondrial and
mitophagy flux analysis; in vivo assessment of muscle
contractility; force measurements on single isolated myofibers; and
analysis of aerobic respiration in intact skeletal muscle tissue by
microplate respirometry. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions
to each respective topic, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls. Cutting
edge and practical, Skeletal Muscle Regeneration in the Mouse:
Methods and Protocols is an essential laboratory reference for
research in skeletal muscle growth, damage, repair, degeneration,
and regenerative therapy in the mouse model system.
This volume provides current methods and protocols for gene and
protein delivery based on both lentivirus-generated and
spontaneously released nanovesicles. Lentiviral Vectors and
Exosomes as Gene and Protein Delivery Tools guides readers through
methods on macromolecule delivery and chapters describe the
LV-based protocols of gene engineering. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Lentiviral
Vectors and Exosomes as Gene and Protein Delivery Tools aims to
ensure successful results in the further study of this vital field.
This volume focuses on the use of system genetic methods and the
use of murine models to study the role of gene variants and
environmental factors on human health and disease-what is now often
called personalized or precision health care. The protocols in this
book will help readers analyze genetic causes of heritable
variation across a wide range of systems and traits using rodent
models. The chapters in this book are separated into three sections
that cover: 1) resources for systems genetics; 2) tools for
analysis and integration in systems genetics; and 3) systems
genetics use cases. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
tools, step-by-step, readily reproducible protocols, and tips on
troubleshooting and avoiding known pitfalls. Practical and
thorough, Systems Genetics: Methods and Protocols is a valuable
resource for anyone who is interested in this diverse field.
This volume details a valuable collection of protocols and reviews,
such as emerging experimental and theoretical approaches. These
approaches have resulted in a substantial improvement in the
understanding of chromosome architecture. Chromosome Architecture:
Methods and Protocols guides readers through cutting-edge
interdisciplinary methods which allow for an understanding of
architecture of chromosomes with exceptionally enhanced resolution,
both in terms of space and time. Written in the highly successful
Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
laboratory protocols, and tips on troubleshooting and avoiding
known pitfalls. Authoritative and cutting-edge, Chromosome
Architecture: Methods and Protocols aims to ensure successful
results in the further study of this vital field.
This book discusses the molecular, biological, pathological, and
clinical aspects of melanoma, with special emphasis in the new
concepts of melanoma genetics. A multidisciplinary group of experts
in Genetics, Dermatology, Pathology, and Melanoma Medical Oncology
contribute state-of-the-art knowledge in melanoma research and
clinical management, not only exposing the current status of
knowledge of the topics but also providing their personal
experiences and ideas about the future and potential practical
application of the genetic aspects of melanoma. During the last few
years we have witnessed an impressive amount of discoveries in the
field of melanoma genetics which have changed our approach in
understanding the pathogenesis and treatment of this lethal
disease. Genetics of Melanoma is a practical approach to melanoma
genetic mechanisms and their application in the diagnosis and
treatment of this malignancy. It is an essential source of updated
information and a powerful tool for clinicians, pathologists, and
basic scientists who wish to understand, apply, and investigate the
multiple new aspects of melanoma genetics.
The third edition of this volume is aimed at providing both
beginners and more experienced researchers a choice of methods to
isolate and analyze dendritic cells(DC). An introductory review
provides an overview of recent advances in the characterization of
DC subsets in mouse and human. While additional chapters provide
methods to culture human and mouse dendritic cells, protocols for
the isolation of dendritic cells, the isolation of dendritic cell
progenitors from mouse, and the purification of dendritic cells
from human blood. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols,
and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Dendritic Cell Protocols, Third
Edition aims to ensure successful results in the further study of
this vital field.
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