Since four decades, rapid detection and monitoring in clinical and food diagnostics and in environmental and biodefense have paved the way for the elaboration of electrochemical biosensors. Thanks to their adaptability, ease of use in relatively complex samples, and their portability, electrochemical biosensors now are one of the mainstays of analytical chemistry. In particular, electrochemistry has played a pivotal role in the development of transduction methods for biological processes and biosensors. In parallel, the explosion of activity in nanoscience and nanotechnology and their huge success have profoundly affected biosensor technology, opening new avenues of research for electrode materials and transduction. This book provides an overview of biosensors based on amperometry, conductimetry, potentiometry, square-wave voltammetry, impedance, and electrochemiluminescence and describes the use of ultramicroelectrodes for the real-time monitoring and understanding of exocytosis. Areas of particular interest are the use of silver and gold nanoparticles for signal amplification, photocurrent transduction, and aptamer design. Moreover, advanced insights in the innovative concept of self-powered biosensors derived from biofuel cells are also discussed.

Exploring fet Concepts for Lab-on-a-Chip.- Monitoring of Cell Growth, Oxygen and ph in Microfermentors.- Observation of Response to Stimuli in Oscillating Cells Patterned by Microfabricated Structure.- On-Chip Agarose Microchamber (AMC) Array Cell-Cultivation System for Topographical Control of Neural Network.- Integrated Size Exclusion and Reversed-Phase Electrochromatography.- Dispersion Analysis of a Solid-Phase Microextraction Chip by Computational Fluid Dynamics.- Interfacing Microchip CE with Icpms for Element Speciation.- Electrodeless Dielectrophoretic Trapping and Separation of Cells.- SiO2 Nozzle Array-Based Patch-Clamp Microsystem.- A Programmable Cell Assay Platform for Kinetic Studies of a Single Cell.- An Integrated Microdialysis-Based System.- User-Assembly, Fully Integrated Micro Chemical Laboratory using Biochemical IC Chips for Wearable/Implantable Applications.- Microfluidic Reactor Array for Multistep Droplet Reactions.- Single Molecular Detection of DNA/Protein and Its Application to Biochips.- DNA Separation in Gradient of Confined Nanospace Generated by Nanoparticles in a Microchannel.- Self-Assembled Magnetic Colloids for DNA Separations in Microfluidic Devices.- A Microfabricated Device for Separating 200KILO-BASE-PAIR DNA Molecules in 15 Seconds.- Airborn Chemistry Levitated Protein Droplets as a Novel Analytical Tool for Nucleation Screening in Macromolecular Crystallisation.- On-Line Monitoring of Airborne Chemistry in Levitated Droplets: in-SITU Synthesis and Application of Sers Active Ag-Sols For Trace Analysis By Raman Spectrometry.- Chip-To-World Interfaces for High-Throughput Lab-On-A-Chip Devices.- The Mems Modeling System by Collaboration of Multi-Scale Simulators and Application to the Microreactor.- Fem Study of Coulter Counter Wggh Water-Based Adaptable Aperture.- Micromanipulating Magnetic Particles in Microfluidic Systems.- Optimal Design of Micromixer Using Nonhomogeneous Multilayer Laminar Flow.- Computational Simulations of Fluid Flow Dynamics, and Bead Packing in Solid Phase Extraction Microsystems.- The Low-Voltage Cascade EOF Pump: Comparing Theory with Published Data.- Universal Joule Heating Model in Electrophoretic Separation Microchips.- Mathematical Model of Interfacial Layer in Ultra-Fine Liquid Drop Based on Molecular Dynamics Simulation.- Development of Micromixer Based on Instability of Interface Between Two Immiscible Liquids.- Theoretical Study of AC Electroosmotic Flows in Non-Uniformly Charged Microchannels.- Numerical Simulation of Mixing in A Micro-Channel with Non-Uniform Zeta Potential Surface.- Injection of Sample Bands from Open Channels into Packed Separation Columns.- Microscale Measurements of Flow Bounded by Air-Water Interfaces.- Virtual Flow Channel: A Novel Micro-Fluidics System with Orthogonal, Dynamic Control Of Sample Flow Dimensions.- Microfabrication of 3-D Oblique Structures by Inclined UV Lithography.- A New Fabrication Technique of A DNA Electrophoresischip by Silicon Micromachining Technology.- Precision Patterning of PDMS Thin Films: A New Fabrication Method and Its Applications.- Fabrication of Microfluidic Channels with Symmetric Cross-Sections for Integrated NMR Analysis.- Precise and Feasible Fabrication Process of Microfluidic Devices on A Borosilicate Glass Chip.- Laminated Electrodes Chip for Pulse-Immunoassay.- Fabrication of On-Chip Sorter Devices with Sub-Micrometer Scale Channels and Self-Aligned Microelectrodes.- Powder Handling Device for Drug Formulations.- Thermal Micropump Using Surface Tension Imbalance in Microchannels.- Optimization of Electrokinetic Pumps for Chip-Based Chromatographic Separations.- Practical Valves and Pumps for Large-Scale Integration into Microfluidic Analysis Devices.- Electro Osmotic Flow Pump Based on The Corbino Disc Geometry.- A Temperature Controlled Micro Valve for Biomedical Applications Using A Temperature Sensitive Hydrogel.- Electroosmotically Driven Two-Liquid Viscous Pump for Nonconducting...

This book covers theoretical aspects of adsorption, followed by an introduction to molecular simulations and other numerical techniques that have become extremely useful as an engineering tool in recent times to understand the interplay of different mechanistic steps of adsorption. Further, the book provides brief experimental methodologies to use, test, and evaluate different types of adsorbents for water pollutants. Through different chapters contributed by accomplished researchers working in the broad area of adsorption, this book provides the necessary fundamental background required for an academician, industrial scientist or engineer to initiate studies in this area. Key Features Explores fundamentals of adsorption-based separation Provides physical insight into aqueous phase adsorption Includes theory, molecular and mesoscopic level simulation techniques and experiments Describes molecular simulations and lattice-Boltzmann method based models for aqueous phase adsorption Presents state-of-art experimental works particularly addressing removal of "emerging pollutants" from aqueous phase

The second edition defines the tools used in QA/QC, especially the application of statistical tools during analytical data treatment. Clearly written and logically organized, it takes a generic approach applicable to any field of analysis. The authors begin with the theory behind quality control systems, then detail validation parameter measurements, the use of statistical tests, counting the margin of error, uncertainty estimation, traceability, reference materials, proficiency tests, and method validation. New chapters cover internal quality control and equivalence method, changes in the regulatory environment are reflected throughout, and many new examples have been added to the second edition.

"Capillary Gel Electrophoresis and Related Microseparation Techniques" covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity based methodologies, micropreparative aspects, as well as related microseparation methods. It gives readers a better understanding of how to utilize this technology, and determine which method will provide the best technical solutions to their particular problems.

This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology, and biotechnology courses.
Covers all theoretical and practical aspects of capillary gel electrophoresisExcellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity based methodologies, micropreparative aspects, as well as related microseparation methodsTeaches readers how to use the technology, and how to select methods ideal for fundamental problemsCan serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology, and biotechnology courses

This Brief describes in three concise chapters one of the newest 'hot topics' under EU Food Law and Policy: the new Regulation (EU) No 2015/2283 from the European Parliament and by the Council, November 25, 2015, on novel foods, applicable from January 2018. In this work, the Authors discuss the long-time criticized EU Regulation on novel foods ((EC) No 258/1997) and how it has been significantly altered by the adoption of the new regulation. In the first chapter, the Authors provide a comprehensive analysis of the genesis of the new Regulation, its rationale and the policy's goals. In particular, they describe what food business operators shall do in order to get a new product allowed on the EU market, providing updated information on the regulatory developments from the European Food Safety Authority in nanofoods, cloned animals and insect foods. The role of the European Food Safety Authority is also discussed. The second Chapter summarizes the current toxicological studies used to evaluate novel foods safety, which are an extremely important pillar when speaking of food safety and commercial introduction of new products. Finally, the third Chapter discusses the 'history of safe use' approach to the problem of novel foods, and factors such as consumption period analysis, preparation advices and processes, intake levels, nutritional composition, and results of animal studies. Food lawyers, professionals and auditors working in the area of official inspections, quality assurance, food traceability, and international regulation, both in academia and industry, will find this Brief an important account.

Chemical Analysis provides non invasive and micro-analytical techniques for the investigation of cultural heritage materials. The tools and techniques, discussed by experts in the field, are of universal, sensitive and multi-component nature.

This book is devoted to the characterization of Maillard reaction products using mass spectrometry (MS)-based technologies. The Maillard reaction is a well-known non-enzymatic reaction between reducing sugars and proteins, and one of the most important reactions in food sciences. The authors explore different MS-based technologies to systematically investigate the Maillard reaction from amino acids, peptides and proteins. By using amino acid/peptide-sugar models, the authors also show how reactants, temperature and time affect the Maillard reaction. In this book, readers will learn more about glucosylation, and how it can improve functional properties of food proteins.

This book is open access under a CC BY 4.0 license. This volume focuses on microscopic plastic debris, also referred to as microplastics, which have been detected in aquatic environments around the globe and have accordingly raised serious concerns. The book explores whether microplastics represent emerging contaminants in freshwater systems, an area that remains underrepresented to date. Given the complexity of the issue, the book covers the current state-of-research on microplastics in rivers and lakes, including analytical aspects, environmental concentrations and sources, modelling approaches, interactions with biota, and ecological implications. To provide a broader perspective, the book also discusses lessons learned from nanomaterials and the implications of plastic debris for regulation, politics, economy, and society. In a research field that is rapidly evolving, it offers a solid overview for environmental chemists, engineers, and toxicologists, as well as water managers and policy-makers.

This brief introduces the classification and mechanism of density gradient ultracentrifugation (DGUC) method with rich examples showing the versatility of such an efficient separation technique. It also gives a strict mathematical description and a computational optimization model to predict the best separation parameters for a given colloidal system. The concept of "Lab in a tube" is proposed in the last chapter, which allows the size-property relationship investigation, synthetic optimization and reaction/assembly mechanism exploration etc.

This book outlines methods to improve functioning of these polymer based devices - in particular, the multi-faceted cognition of these materials. In situ electrochemical techniques are studied to elucidate redox switching between non-conducting and conducting states. The book examines the advantages of combinations of in situ electrochemical techniques in a hyphenated mode for analyzing conducting polymers.

Presents the theory and applications of Toroidal Capillary, Microchip, and Slab Electrophoresis to analytical chemists across a range of disciplines Written by one of the developers of Toroidal Capillary Electrophoresis (TCE), this book is the first to present this novel analytical technique, in detail, to the field of analytical chemistry. The exact expressions of separation efficiency, resolution, peak capacity, and many other performance indicators of the open and toroidal layouts are presented and compared. Featuring numerous illustrations throughout, Open and Toroidal Electrophoresis: Ultra-High Separation Efficiencies in Capillaries, Microchips and Slabs offers chapters covering: Solvents and Buffer Solutions; Fundamentals of Electrophoresis; Open Layout; and Toroidal Layout. Confronting Performance Indicators is next, followed by chapters on High Voltage Modules and Distributors; Heat Removal and Temperature Control; and Detectors. The book finishes with an examination of the applications of Toroidal Electrophoresis. The first book to offer a detailed account of Toroidal Electrophoresis--written by one of its creators Compares the toroidal layouts with the well-established open layouts of the three most used platforms (Capillary, Microchip, and Slab) Provides solutions to many of the experimental issues arising in electromigration techniques and discusses the voltage distributors and detectors that are compatible with the toroidal layouts Richly illustrated with a large number of useful equations showing the relationships between important operational parameters and the performance indicators Open and Toroidal Electrophoresis is aimed at method developers and separation scientists working in clinical analysis, and food analysis, as well as those in pharmacology, disease biomarker applications, and nucleic acid analysis using the Capillary, Microchip, or slab Platform. It will also benefit undergraduate and graduate students of inorganic analytical chemistry, organic analytical chemistry, bioanalysis, pharmaceutical sciences, clinical sciences, and food analysis.

This Brief describes the chemical features of canned food products and gives background information on the technology of canning foods. It explains how canned foods are different from other packaged foods, and illustrates and discusses their unique properties, including risks and failures. Canned foods are usually considered to offer a particularly long shelf-life and durability. An understanding of their properties and influences on their durability is therefore of great importance in the industrial production, and this Brief offers a compact introduction to this topic. The authors focus on thermally-preserved foods. They explain that the right choice of thermal treatment method (e.g. pasteurisation, sterilisation) as well as process parameters (e.g. time, temperature) is additionally influenced by criteria such as pH, water content, the presence and concentration of fatty molecules, of calcium, etc. So-called 'survival curves' can help in determining the methodology of choice, and the Brief introduces the reader to this concept. The authors also address defects and failures. They introduce selected indicators, which can help identifying failures of the entire food/packaging system, and demonstrate how image and visual analysis can be applied in quality controls. The explanations and industrial production of canned foods are exemplified with the case of canned tomato sauces and beans.

To ensure food quality and safety food, professionals need a knowledge of food composition and characteristics. The analysis of food product is required for quality management throughout the developmental process including the raw materials and ingredients, but food analysis adds processing cost for food industry and consumes time for government agencies. Advances in Noninvasive Food Analysis explores the potential and recent advances in non-invasive food analysis techniques used to ensure food quality and safety. Such cost-reducing and time-saving non-destructive food analysis techniques covered include, Infrared, Raman Spectroscopy, and Nuclear Magnetic Resonance. The book also covers data processing and modelling. Features: Covers the advent of non-invasive, non-destructive methods of food analysis Presents such techniques as near and mid infrared, Raman Spectroscopy, and Nuclear Magnetic Resonance Describes the growing role of nanotechnology in non-invasive food analysis Includes image analysis and data processing and modelling required to sort out the data The prime for this book are food professionals working in industry, control authorities and research organizations that ensure food quality and safety as well as libraries of universities with substantial food science programs, food companies and food producers with research and development departments. Also available in the Contemporary Food Engineering series: Advances in Food Bioproducts, Fermentation Engineering and Bioprocessing Technologies , edited by Monica Lizeth Chavez Gonzalez, Nagamani Balagurusamy, Christobal N. Aguilar (ISBN 9781138544222) Advances in Vinegar Production, edited by Argyro Bekatorou (ISBN 9780815365990) Innovative Technologies in Seafood Processing, edited by Yesim Ozogul (ISBN 9780815366447)

To understand hydrochemistry and to analyze natural as well as man-made impacts on aquatic systems, hydrogeochemical models have been used since the 1960’s and more frequently in recent times. Numerical groundwater flow, transport, and geochemical models are important tools besides classical deterministic and analytical approaches. Solving complex linear or non-linear systems of equations, commonly with hundreds of unknown parameters, is a routine task for a PC. Modeling hydrogeochemical processes requires a detailed and accurate water analysis, as well as thermodynamic and kinetic data as input. Thermodynamic data, such as complex formation constants and solubility-products, are often provided as databases within the respective programs. However, the description of surface-controlled reactions (sorption, cation exchange, surface complexation) and kinetically controlled reactions requires additional input data. Unlike groundwater flow and transport models, thermodynamic models, in principal, do not need any calibration. However, considering surface-controlled or kinetically controlled reaction models might be subject to calibration. Typical problems for the application of geochemical models are: • speciation • determination of saturation indices • adjustment of equilibria/disequilibria for minerals or gases • mixing of different waters • modeling the effects of temperature • stoichiometric reactions (e.g. titration) • reactions with solids, fluids, and gaseous phases (in open and closed systems) • sorption (cation exchange, surface complexation) • inverse modeling • kinetically controlled reactions • reactive transport Hydrogeochemical models depend on the quality of the chemical analysis, the boundary conditions presumed by the program, theoretical concepts (e.g.

This thesis describes longitudinal nuclear relaxation measurements of solid 129Xe near 77 K with previously unattainable reproducibility, and demonstrates differences in relaxation, dependent upon the way in which the solid is condensed. These results are directly applicable to the generation and storage of large quantities of hyperpolarized 129Xe for various applications, such as lung magnetic resonance imaging (MRI). The thesis features a sophisticated theoretical approach to these data sets, including modifications to a well-established Raman-phonon scattering theory that may explain the larger scatter in and discrepancies with previous work.

This book discusses the paradigm-shifting phenomenon of intrinsically disordered proteins (IDPs) and hybrid proteins containing ordered domains and functional IDP regions (IDPRs). The properties of IDPs and IDPRs are highly complementary to those deriving from the presence of a unique and well-defined three-dimensional fold. Ignored for a long time in high-resolution studies of proteins, intrinsic protein disorder is now recognized as one of the key features for a large variety of cellular functions, where structural flexibility presents a functional advantage in terms of binding plasticity and promiscuity and this volume explores this exciting new research. Recent progress in the field has radically changed our perspective to study IDPs through NMR: increasingly complex IDPs can now be characterized, a wide range of observables can be determined reporting on the structural and dynamic properties, computational methods to describe the structure and dynamics are in continuous development and IDPs can be studied in environments as complex as whole cells. This volume communicates the new exciting possibilities offered by NMR and presents open questions to foster further developments. Intrinsically Disordered Proteins Studied by NMR Spectroscopy provides a snapshot to researchers entering the field as well as providing a current overview for more experienced scientists in related areas.

The series Structure and Bonding publishes critical reviews on topics of research concerned with chemical structure and bonding. The scope of the series spans the entire Periodic Table and addresses structure and bonding issues associated with all of the elements. It also focuses attention on new and developing areas of modern structural and theoretical chemistry such as nanostructures, molecular electronics, designed molecular solids, surfaces, metal clusters and supramolecular structures. Physical and spectroscopic techniques used to determine, examine and model structures fall within the purview of Structure and Bonding to the extent that the focus is on the scientific results obtained and not on specialist information concerning the techniques themselves. Issues associated with the development of bonding models and generalizations that illuminate the reactivity pathways and rates of chemical processes are also relevant. The individual volumes in the series are thematic. The goal of each volume is to give the reader, whether at a university or in industry, a comprehensive overview of an area where new insights are emerging that are of interest to a larger scientific audience. Thus each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years should be presented using selected examples to illustrate the principles discussed. A description of the physical basis of the experimental techniques that have been used to provide the primary data may also be appropriate, if it has not been covered in detail elsewhere. The coverage need not be exhaustive in data, but should rather be conceptual, concentrating on the new principles being developed that will allow the reader, who is not a specialist in the area covered, to understand the data presented. Discussion of possible future research directions in the area is welcomed. Review articles for the individual volumes are invited by the volume editors. Readership: research scientists at universities or in industry, graduate students Special offer for all customers who have a standing order to the print version of Structure and Bonding, we offer free access to the electronic volumes of the Series published in the current year via SpringerLink.

This volume builds on the previous two editions, Environmental Photochemistry Part I and Part II, which reflect the diverse range of activities in this highly dynamic research field. The chapters cover fundamental topics, from photocatalyst materials, surface-modified materials, reaction kinetics and reactor modelling, to translational research activities on chemical synthesis, energy conversion and water treatment. The applications of the new generation of LED irradiation sources and spectroscopic methods for elucidating reaction pathways are also covered in detail. This new volume maintains the ethos of the previous editions by further contributing to readers’ understanding of photochemical and photocatalytic processes for environmental applications. 

The series Topics in Current Chemistry presents critical reviews of the present and future trends in modern chemical research. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. Review articles for the individual volumes are invited by the volume editors. Readership: research chemists at universities or in industry, graduate students.

This book presents an Ultrafast Low-Energy Electron Diffraction (ULEED) system that reveals ultrafast structural changes on the atomic scale. The achievable temporal resolution in the low-energy regime is improved by several orders of magnitude and has enabled the melting of a highly-sensitive, molecularly thin layer of a polymer crystal to be resolved for the first time. This new experimental approach permits time-resolved structural investigations of systems that were previously partially or totally inaccessible, including surfaces, interfaces and atomically thin films. It will be of fundamental importance for understanding the properties of nanomaterials so as to tailor their properties.

The book describes the direct problems and the inverse problem of the multidimensional Schrödinger operator with a periodic potential. This concerns perturbation theory and constructive determination of the spectral invariants and finding the periodic potential from the given Bloch eigenvalues. The unique method of this book derives the asymptotic formulas for Bloch eigenvalues and Bloch functions for arbitrary dimension. Moreover, the measure of the iso-energetic surfaces in the high energy region is construct and estimated. It implies the validity of the Bethe-Sommerfeld conjecture for arbitrary dimensions and arbitrary lattices. Using the perturbation theory constructed in this book, the spectral invariants of the multidimensional operator from the given Bloch eigenvalues are determined. Some of these invariants are explicitly expressed by the Fourier coefficients of the potential. This way the possibility to determine the potential constructively by using Bloch eigenvalues as input data is given. In the end an algorithm for the unique determination of the potential is given.

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint� for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

Tian Lu's dissertation describes major advances in ultrathin-layer chromatography (UTLC), liquid chromatography and surface-assisted laser desorption ionization (SALDI), and matrix-enhanced SALDI (ME-SALDI) mass spectrometry. Lu describes the fabrication of electrospun polyvinyl alcohol (PVA) UTLC plates using an in-situ crosslinking electrospinning technique. The author improved the efficiency of PVA plates greatly compared to the efficiency of silica HPTLC plates. Also highlighted in this thesis is an edge-plane based ordered-carbon surface that provides unique selectivity in liquid chromatography. Further developments include polar analytes, such as amino acids, nucleotides and nucleosides which can be well-retained and separated in the edge-plane ordered-carbon stationary phase. Also, the author studied and detected mass spectra of organic polymers as high as 900,000 Da, the highest molecular weight that has been studied by SALDI to date using the carbon nanofibrous substrate. This thesis has led to a number of publications in high-impact journals.

This volume offers concepts, methods and case studies of innovative and evolving technologies in the area of watershed assessment. Topics discussed include: (1) Development and applications of geospatial, satellite imagery and remote sensing technologies for land monitoring; (2) Development and applications of satellite imagery for monitoring inland water quality; (3) Development and applications of water sensor technologies for real-time monitoring of water quantity and quality; and (4) Advances in biological monitoring and microbial source tracking technologies. This book will be of interest to graduate students and researchers involved in watershed science and environmental studies. Equally, it will serve as a valuable guide to experts in government agencies who are concerned with water-availability and water-quality issues, and engineers and other professionals involved in the design of land- and water-monitoring systems.