This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Of the thousands of biomarkers that are currently being discovered, relatively few are being validated for further applications, and the potential of a biomarker can be quite difficult to evaluate. To aid in this imperative research, Dr. Kewal K. Jain's Handbook of Biomarkers thoroughly describes many different types of biomarkers and their discovery using various "-omics" technologies, such as proteomics and metabolomics, along with the background information needed for the evaluation of biomarkers as well as the essential procedures for their validation and use in clinical trials. With biomarkers described first according to technologies and then according to various diseases, this detailed book features the key correlations between diseases and classifications of biomarkers, which provides the reader with a guide to sort out current and future biomarkers.

Comprehensive and cutting-edge, The Handbook of Biomarkers serves as a vital guide to furthering our understanding of biomarkers, which, by facilitating the combination of therapeutics with diagnostics, promise to play an important role in the development of personalized medicine, one of the most important emerging trends in healthcare today.

Pain is an unfortunate daily experience for many individuals. Chronic pain -- lasting six or more months -- is suffered by approximately 30% of the population in the United States. These individuals wake up, function during the day and go to sleep, trying to keep pain at a minimum while, at the same time, maintaining some quality of life. They may make frequent visits to the doctor and the pharmacy. When they find relief, it is usually short-lived and comes at a cost such as dependence on narcotic medications or complete limitation of activity. Pain often becomes the central point of their existence. This practice guide describes an approach to psychological evaluation of the chronic pain patient who is being considered for surgery. A large body of research is accumulating which demonstrates that the outcome of surgical procedures aimed at chronic pain relief can be strongly influenced by psychological and emotional factors. This approach, termed "presurgical psychological screening" (PPS) uses interview and testing techniques to identify emotional, behavioral, and psychosocial difficulties which have been demonstrated to negatively impact surgical outcome. Studies show that even patients with clearly identifiable pathophysiology may respond poorly to surgery, due to issues such as pain sensitivity, medication dependence, rewards for pain behavior and personality style. Thus, some insurance carriers, rehabilitation nurses and state worker's compensation systems are encouraging, or even requiring, presurgical psychological screening in cases of surgery designed to relieve chronic pain. The first to present a comprehensive, unified approach to PPS in chronic pain syndromes, this text is designed to provide the behavioral health practitioner, as well as the trainee, with all the tools and information necessary to conduct PPS evaluations. It identifies a multitude of risk factors for poor surgical outcome and reviews research associated with each risk factor. Hands-on techniques for eliciting information from the patient about risk factors is also detailed. Toward this end, the practice guide also contains a number of forms and session outlines which can be directly utilized, or which can be altered to fit readers' needs. Models for weighing and combining surgical outcome risk factors are also provided. Thus, practitioners are able to reach valid and reliable predictions of surgical results. Finally, the text provides outlines of psychological interventions which can facilitate surgical outcome as well as surgical treatment alternatives. Upon completion of this practice guide, readers should be able to begin providing PPS evaluations which are scientifically valid, clinically sound, and which result in significant overall improvement in the treatment of chronic pain syndromes.

Sensor technologies and applications are evolving rapidly driven by the demand for new sensors for monitoring and diagnostic purposes to enable improvements in human health and safety. Simultaneously, sensors are required to consume less power, be autonomous, cost less, and be connected by the Internet of Things. New sensor technologies are being developed to fulfill these needs. This book reviews the latest developments in sensor technology and gives the reader an overview of the state-of-the-art in key areas, such as sensors for diagnostics and monitoring. Features Provides an overview of sensor technologies for monitoring and diagnostics applications. Presents state-of-the-art developments in selected topics for sensors that can be used for monitoring and diagnostics in future healthcare, structural monitoring, and smart environment applications. Features contributions from leading international experts in both industry and academia. Explores application areas that include medical diagnostics and screening, health monitoring, smart textiles, and structural monitoring.

Drs. Leonard Scinto and Kirk Daffner provide a comprehensive survey of new diagnostic approaches to Alzheimer's disease. The authoritative contributors critically survey the most promising current research on early diagnostic markers for Alzheimer's disease, including the elucidation of changes in the brain revealed by structural and functional neuroimaging, as well as the characteristic patterns of cognitive decline that are documented by sensitive neuropsychological tests, various genetic markers, and biological assays. Early Diagnosis of Alzheimer's Disease illuminates the complex issues surrounding the search for early markers of this increasingly widespread disease. It will establish a new standard reference guide for all those working with Alzheimer's patients.

There are individual and professional factors that have the greatest impact on the preservation of work ability for people of advanced age, and people with chronic health issues and disabilities. This text examines the demographic trends in the population and shows that proper functioning in their lives is only possible if people are provided with appropriate psychosocial and physical working conditions. This publication discusses physiological changes that occur with age and influence work performance. It presents the concept of applying the International Classification of Functioning, Disability and Health (ICF) in order to assess the work ability of people with disabilities. It shows, using examples and case studies, that practical activities aimed at appropriate working conditions for people of advanced age, and people with health issues and disabilities, will have excellent work ability. Features Presents a comprehensive approach to workplace tolerance for people with health issues Offers an innovated in-depth evaluation of the Work Ability Index (WAI) Presents applying the International Classification of Functioning, Disability and Health (ICF) to access the work ability of people with disabilities Outlines actions that should be taken to maintain work ability for people of advanced age or with disabilities Provides real case studies and analysis. "Demographic changes, especially those related to aging, belong to the main changes occurring in the contemporary working environment. Thus, preserving work ability in the older population as well as [populations with] health concerns are among the most important challenges of today. Accordingly, the monograph fits into the most recent issues of [this] public health area, focusing on work ability in populations at risk of social exclusion, also occupational exclusion. These are older people, persons with chronic diseases and those with different types of disabilities. In my opinion, highlighting the problem of work ability in the context of aging by the authors, in such a broad understanding, is extremely valuable and useful and the presented research outcome is an authentic and very much expected contribution to the discussion on this issue. The topic has been thoroughly presented, which undoubtedly results from the interdisciplinarity of the group of authors including specialists in work physiology, physicians and physiotherapists. The monograph provides a very measurable and reliable knowledge in this field. Combining the theory with the presentation of the authors' research outcomes is an unquestionable advantage of the monograph...". -Jolanta Walusiak-Skorupa, Nofer Institute of Occupational Medicine, Lodz, Poland

With a step-by-step method for accurate interpretation of the ECG, this third edition of Rapid ECG Interpretation describes a systematic approach consistent with the changes in cardiology practice over the past decade. All diagnostic ECG criteria are given with relevant and instructive ECGs, providing a quick review or refresher for proficiency tests and for physicians preparing for the ECG section of the Cardiovascular Diseases Board Examination. This edition contains over 320 ECGs and instructive illustrations, including 81 new ECG tracings. Clear and concise 11-step methods are set out in a user-friendly synopsis format.

For every physician that interprets ECGs, there is great need to understand a vast amount of information regarding the technique. That the basics of the technique have changed little over the last 100 years means that there is a huge amount of subtle detail that must be learnt to enable its effective use as a diagnostic test. The ECG technique is critical for deciding upon further diagnostic procedures and therapeutic interventions (notably coronary angiography, PTCA, stenting, coronary artery bypass grafting, pacemaker insertion, ablation, electroconversion etc). Without attaining the skills to practice the ECG procedure and knowledge of its diagnostic value - skills often overlooked during medical training - physicians will be unlikely to use it to the benefit of their patients.

The only case-based guide to electromyography-back in a fully revised and updated New Edition! This practical resource examines how to approach, diagnose, and manage the most commonly encoun-tered disorders in the EMG laboratory. Based on actual cases, it correlates patient history, physical exam, EMG findings, relevant anatomy, treatment, and follow-up to help readers sharpen their clinical problem-solving skills. New cases have been added, and every case includes the latest advances in knowledge and technique. Features study questions, answers, and clinical discussions of how experts manage cases to help readers work through the problems presented. Summarizes the results of nerve conduction studies and EMG data with standardized tables. Includes more than 200 relevant imaging studies and anatomic figures. Makes information easy to find with a uniform chapter organization. Offers a consistent approach to electromyography based on Dr. Katirji's broad knowledge and clinical experience. 7 new case studies, including Hereditary Neuropathy with Liability to Pressure Palsy, Ischemic Monomelic Neuropathy, and Myotonic Dystrophy. Three new chapters on Nerve Conduction Studies, Needle EMG Examination, and Specialized Procedures. Many new and revised figures that clarify complex information.

Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. With 700+ questions and 300 illustrations, the go-to ARDMA study guide delivers the updated content and hands-on practice you need to ace the exam Fully updated with the latest terminology and concepts, Vascular Technology Examination PREP, Second Edition is the go-to learning tool for anyone taking ARDMS (R) Vascular Technology registry exam. Covering all aspects of vascular disease and testing, it combines vascular principles with high-yield review questions that boost your comprehension and retention. This unparalleled guide provides you with a thorough look at every vascular region, including cerebrovascular, venous, peripheral arterial, and abdominal/visceral. Full-color illustrations, tables, and sonographic images reinforce core concepts, and a dedicated chapter covers the latest perspectives in quality assurance, with detailed information on patient and practitioner safety guidelines. * UPDATED: questions, images, terminologies, and protocols * NEW: Focus on new technologies, including IVUS, carotid stent criteria, renal stent criteria, vector flow imaging, Chromaflow, Angiojet, and ultrafast Doppler

In 1994 I coedited a book, Molecular Pathology: Ap- efforts. It should be evident that molecular pathology and proaches to Diagnosing Human Disease in the Clinical diagnostics impact almost every conceivable subspecialty Laboratory, that coined the phrase "molecular pathology" in laboratory medicine and, particularly in the case of to refer to applications of molecular biology in the tradi- pharmacogenomics, have led to the development of new tional areas of laboratory medicine. That compilation of areas of investigation. clinical molecular techniques included 11 chapters and an What is less evident is the burden placed upon the epilogue on "New Directions for the Clinical Laboratory. " laboratorians directing these efforts. Issues regarding Chapter headings included: automation, neoplasia, heri- training, certification, continuing education, and re- table diseases, and infectious diseases. bursement (just to name a few) have demanded enormous Three years later, in 1997, Bill Coleman and Greg Tson- time and effort from professional organizations and g- galis edited Molecular Diagnostics: For the Clinical ernmental agencies. In addition, because conventional Laboratorian, which contained 18 chapters and introduced approaches cannot always be applied to molecular di- the new areas of RT-PCR and In Situ PCR, and included nostics, unique solutions for quality assurance and quality a section on gene therapy. Now, ten years after the intro- control must be developed. These challenges have eng- duction of molecular pathology, we are looking at the dered committees, subcommittees, taskforces, and second edition of Bill Coleman and Greg Tsongalis' text.

This book disseminates information on paper-based diagnostics devices and describes novel paper materials, fabrication techniques, and Basic Paper-based microfluidics/electronics theory. The section on sample preparation, paper-based electronics/sensors for developing paper-based point-of-care (POC) systems also contains detailed descriptions. In the application sections this book covers sensing technique for DNA/RNA, bacteria/virus and integration of lateral flow assay. The book provides deep understanding and knowledge of paper-based diagnostic device development in terms of concept, materials, fabrication and applications.

This text atlas, now in its second edition, presents in simplest form the basic diagnostic criteria used by the electron microscopist in studying neoplasms and other diseases encountered in the routine practice of pathology. Every field of electron microscopy is covered and low magnification plates are juxtaposed with higher magnifications to illustrate diagnostic features.The largest section of the book is devoted to neoplasms as this is the area in which most diagnostic problems occur. Renal glomerular disease is another important category in which ultrastructural study may be critical in diagnosis; infectious diseases, especially those of viral, protozoan, and unusual bacterial etiologies, are a third area in which electron microscopy may be used to establish or susbstantiate a diagnosis. All of these areas are comprehensively covered with concise, readable text and more than 800 first-quality images. This book is the preeminent reference for pathologists needing current information on the role of ultrastructure in diagnostic pathology.

From probe design to applications in clinical settings, this book provides a diverse set of instructive examples, guided by experts in the field who offer easy-to-follow experimentals. The book first offers an introduction to the basic principles of fluorescence and then describes applications of fluorogenic probes in real-time PCR, which currently is the gold standard for quantitative DNA and RNA analysis. Coverage extends the potential of realtime as well as advocates simplifications of the probe technologies. It also presents a new simplified molecular beacon design, EasyBeacons, and demonstrates the utility in DNA methylation profiling.

This book acts as a clinical manual for the diagnostician who cannot turn to reference books when the morphology or immunophenotype are atypical. This volume presents a logical practical approach to the diagnosis of blood disorders, both neoplastic and reactive, and other diagnostic applications of flow cytometry in non-neoplastic haematology diagnosis. Illustrations are provided throughout with worked examples.

First published in 2002. Routledge is an imprint of Taylor & Francis, an informa company.

There is a new trend in anti-cancer therapeutics development: a targeted therapy and precision medicine that targets a subgroup of patients with specific biomarkers. An in vitro diagnostic (IVD) assay is required to identify a subgroup of cancer patients who would benefit from the targeted therapy, or not likely benefit, or have a high risk of side effects from the specific drug treatment. This IVD or medical device is called a companion diagnostic (CDx) assay. It is key to have a robust CDx assay or device for the success of targeted therapy and precision medicine. This book covers the technical, historical, clinical, and regulatory aspects of CDx in precision medicine. Clearly, more and more newly developed oncology drugs will require accompanying CDx assays, and this book, with chapters contributed by renowned oncologists, provides a comprehensive foundation for the knowledge and application of CDx for precision medicine.

The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. The detection of parasites in clinical cases, companion and farm animals, and in the environment is relatively easy since many of them are visible to the naked eye, and those that are not are readily detected by light microscopy. Fungal infections can similarly be determined. Bacteria are somewhat harder to detect. Although their presence can frequently be detected by light microscopy, differential diagnosis, beyond their gross morphology, is almost always impossible. However, most bacterial pathogens can be cultured in the laboratory and can be accurately identified by combinations of a series of simple tests such as morphology, staining, antibiotic sensitivity, biochemical analyses, nutrient dependence, and phage sensitivity. Viruses, however, have proved much more difficult; their size and absolute dependence of the host cell for propagation have rendered useless the methods traditionally used for other microorganisms. Until the development of tissue culture in the middle of this century, diagnosis was entirely dependent on the skill and experience of the clinician. But this was an unreliable process since many of the common virus infections exhibit similar clinical symptoms, such as coryza, exanthema, vomiting, diarrhea, neuralgia, and lethargy. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.

Objective Biometric Methods for the Diagnosis and Treatment of Nervous System Disorders provides a new and unifying methodological framework, introducing new objective biometrics to characterize patterns of sensory motor control underlying symptoms. Its goal is to radically transform the ways in which disorders of the nervous system are currently diagnosed, tracked, researched and treated. This book introduces new ways to bring the laboratory to the clinical setting, to schools and to settings of occupational and physical therapy. Ready-to-use, graphic user interfaces are introduced to provide outcome measures from wearable sensors that automatically assess in near real time the effectiveness of interventions. Lastly, examples of how the new framework has been effectively utilized in the context of clinical trials are provided.

This book describes the latest methods of oncological and hematological diagnostics such as immunological, molecular genetic and histological essays. All methods are described in principle in their different variations and compared in their effectiveness and cost. At the end of each chapter a detailed description of the "how-to-do" is given. The book is written for scientists, clinicians and personnel from research laboratories, specialised laboratories and routine diagnostic laboratories in hospitals. It satisfies the increased demand for information on new methods in hematology and oncology.

This volume is the first collection of applications of proteomics to analyze various human body fluids. Proteomics of Human Bodyfluids consists of two parts. The first provides basic principles and strategies for proteomic analysis of human body fluids. The second offers more details regarding methodologies and recent findings and clinical applications of each specific type of human body fluids.

100 Diagnostic Challenges in Clinical Medicine is composed of one hundred well-illustrated clinical scenarios and their appropriate investigations. A wide variety of specialties are covered including cardiology, neurology, dermatology, endocrinology, tropical medicine, haematology, metabolic medicine, radiology, ophthalmology, venereology, and infectious diseases. Presenting the relevant investigations corresponding to each case in an interesting and easy-to-read Q&A format concerning diagnosis and management, this book serves as an ideal, and hopefully enjoyable, study aid for medical students and junior doctors who are preparing for clinical examinations in medicine. By solving the problems proposed by these challenging clinical cases, the reader will gain additional practice in diagnosis and treatment strategies.

This is a guide for psychiatry residents, medical students, and trainees in clinical psychology, social work and nursing. Druss examines the challenges and rewards of establishing a work alliance with patients and demonstrates how therapy can provide a safe means for them to handle their experiences. Chapter topics include: creating a working alliance, initial sessions, conversation reactions, body image, the spiritual life of patients, the goals of patients and therapists, and patients who return to therapy.

The "information explosion" in recent decades has made it impossible for practicing physicians (even specialists) to keep up with all the information potentially at their disposal. As a result, it is not surprising that empirical studies have shown that physicians do not always make optimal decisions. Thus, medical expert systems are now available to support - not replace - physicians and healthcare providers in their goal of providing the best possible healthcare to every patient. Knowledge Engineering in Health Informatics is a guide to the creation of such systems. Presenting the core material for courses such as Medical Knowledge Engineering and Expert System Development, it allows non-experts to make diagnostic decisions with the precision and accuracy of medical experts thanks to the help of the computer.

This unique reference provides a pragmatic approach to the development of successful commercial immunodiagnostic products based on enzyme immunoassay technology. Presenting both basic and applied principles, Enzyme Immunoassays gathers information on all aspects of this process, from the initial conceptualization to the introduction of the product to the market. Skillfully organized into two parts, this comprehensive resource begins by discussing basic concepts, such as the classification, structure and function of antibodies and the properties and characteristics of the most widely used enzymes in immunoassays. The second section examines the industrial product development process, including a detailed outline of various protocols and points to be considered for designing a successful product. Described in this section are the various formats available for product development, reagent formatting and assay development, data processing, standardization, scale up, and commercial manufacture of the product. Also included are the various regulatory requirements, the importance of good laboratory and manufacturing (GLP and GMP) practices, and international requirements such as the ISO 9000 certification process. Finally, information is presented for the benefit of entrepreneurs who would like to venture into this exciting field with their own company. With over 75 illustrations and 40 tables, Enzyme Immunoassays is an incomparable reference for scientists, technologists, and analytical chemists working in the field of immunodiagnostics at all levels, as well as for upper-level undergraduate and graduate students in life sciences fields in order to understand the basic concepts and principlesinvolved in developing rapid assay techniques.