This book comprises of the edited proceedings of a seminar on environmental modelling. It provides a comprehensive overview of current environmental problems and the progress achieved in their numerical simulation.

This is the first publication to offer a comprehensive and balanced view of atmospheric acidity. It is organised in three sections. The first part consists of reviews of sources of acidic compounds, the second part outlines the environmental consequences and the final part discusses the technological, legal and political aspects of control strategies.

Ecotoxicology is a relatively new scientific discipline. Indeed, it might be argued that it is only during the last 5-10 years that it has come to merit being regarded as a true science, rather than a collection of procedures for protecting the environment through management and monitoring of pollutant discharges into the environment. The term 'ecotoxicology' was first coined in the late sixties by Prof. Truhaut, a toxicologist who had the vision to recognize the importance of investigating the fate and effects of chemicals in ecosystems. At that time, ecotoxicology was considered a sub-discipline of medical toxicology. Subsequently, several attempts have been made to portray ecotoxicology in a more realistic light. Notably, both Moriarty (1988) and F. Ramade (1987) emphasized in their books the broad basis of ecotoxicology, encompassing chemical and radiation effects on all components of ecosystems. In doing so, they and others have shifted concern from direct chemical toxicity to humans, to the far more subtle effects that pollutant chemicals exert on natural biota. Such effects potentially threaten the existence of all life on earth. Although I have identified the sixties as the era when ecotoxicology was first conceived as a coherent subject area, it is important to acknowledge that studies that would now be regarded as ecotoxicological are much older.

Poisonous mushrooms have fascinated scientists and laypersons alike for thousands of years. Almost all mushroom fatalities are due to the genus Amanita, whose poetic common names (death cap, destroying angel) attest to their lethality. In his classic 1986 book, Theodor Wieland covered the state of our knowledge about the chemistry and biochemistry of the toxins of Amanita mushrooms up until that time, with a particular focus on the decades of chemical research by him and the Wieland dynasty (including his father, brother, brother-in-law, and cousin). Wieland's book is now mainly of historical interest, with its exhaustive overview of the early chemical studies done without benefit of methods taken for granted by modern chemists. This book is a complete top-to-bottom revision of Wieland's 1986 book. The material covers history, chemistry, and biology with equal thoroughness. It should be of interest to natural products chemists and biologists, professional and amateur mycologists, and toxicologists. The three scientific fields that are most relevant to the book are natural products chemistry, mycology, and fungal molecular genetics. Dr. Walton is an expert in all three. To maximize the broad utility and appeal of the book, care has been taken to define all technical terms specific to a particular discipline, so that, for example, mycologists will be able to understand the relevant chemistry, and chemists will be able to understand the relevant fungal biology.

A well-structured and comprehensive summary of the strategies and several case studies for applying molecular plant genomics in the fields of plant ecotoxicology and plant ecology. With an increasing number of plant genome projects now being completed, there arises the need to develop plant functional genomics. The book concentrates on ecological functions and relates molecular stress responses and signalling pathways to environmental interactions. This paves the way for uncovering new mechanisms of plant fitness, population dynamics and evolution, and new possibilities for plant breeding and sustainable agriculture. Topics covered include: definition and up-scaling of molecular ecotoxicology; signalling substances, enzymes and genes involved in defence against pathogens, xenobiotics, ozone, UV-B and further environmental stressors; and manipulation of plant signal transduction by soil bacteria.

This book provides information on best practices and new thinking regarding the validation of alternative methods for toxicity testing. It covers the validation of experimental and computational methods and integrated approaches to testing and assessment. Validation strategies are discussed for methods employing the latest technologies such as tissue-on-a-chip systems, stem cells and transcriptomics, and for methods derived from pathway-based concepts in toxicology. Validation of Alternative Methods for Toxicity Testing is divided into two sections, in the first, practical insights are given on the state-of-the-art and on approaches that have resulted in successfully validated and accepted alternative methods. The second section focuses on the evolution of validation principles and practice that are necessary to ensure fit-for-purpose validation that has the greatest impact on international regulatory acceptance of alternative methods. In this context validation needs to keep pace with the considerable scientific advancements being made in toxicology, the availability of sophisticated tools and techniques that can be applied in a variety of ways, and the increasing societal and regulatory demands for better safety assessment. This book will be a useful resource for scientists in the field of toxicology, both from industry and academia, developing new test methods, strategies or techniques, as well as Governmental and regulatory authorities interested in understanding the principles and practicalities of validation of alternative methods for toxicity testing.

There are various species of fungi which like to grow on food, thereby releasing toxins which might bear a health risk for the consumer. All foods which have been reported to be contaminated with mycotoxins are listed, including data on the degree of contamination, the concentration of the toxins and the country of origin and/or detection of the contaminated food. All relevant toxin producing fungi, their natural occurence, the possible mycotoxicosis, further the biochemical and physiological effects of mycotoxins, their chemical data and toxicity are treated comprehensively. For each mycotoxin, reference is given to the food at risk.

Proceedings of the Third Annual Conference, Washington, DC, USA, 3-4 December 1986

Pesticides in the Natural Environment: Sources, Health Risks, and Remediation presents the direct and indirect impacts of the use of pesticides on the environment, human health, and agriculture. The book explores sustainable alternatives to pesticide use, along with policies for regulations and remediation techniques. Bridging the gap between regulations and the tangible environmental threat, the book proposes practical solutions while also providing important context on the hazards of pesticides. It highlights the influence on climate change, offering a holistic perspective for researchers in environmental science, policymakers, and land managers. The book introduces pesticides and their applications, then goes on to cover their impact on various ecosystems in the natural environment. Health risks are covered, followed by various remediation techniques, such as biological processes, phytoremediation, and chemical treatments.

Preface When you write a book like this after ten years' working as an environmental specialist, you end up with something that reflects your career. Of course, when I started working at the Ministry of the Environment in the Netherlands, I could not foresee that I would now be at TNO, nor that I would have performed research into chlorine, PVC, waste, etc. , that would come to form the basis for this book. But step by step, with some coincidence and with the support of several people - who were probably unaware of the crucial role that, with hindsight, they played - I arrived at a position where I could start to consider this enterprise. At this point I shall try something dangerous - thanking a few of those people who gave that support. At the same time, it is obvious that I cannot mention them all. I hope that those whom I do not mention will forgive me. A first, crucial moment in this sequence of events came quite soon after I joined TNO in 1990. Just a few weeks later, all the senior staff in my section decided to leave in order to set up their own company. I decided to stay at TNO. As a consequence, I had to manage it on my own.

During the past five years work has been in progress to examine and treat the acidity of Loch Fleet in south west Scotland and its catchment waters. This text describes the background to the research, the application and effects of liming, and relates the experience gained in the project to practice and research in other parts of the world. In particular the success achieved in restoring fish populations to the loch is described as well as the consequences for other flora and fauna.

A mycotoxin is a toxin produced by a fungus under special conditions of moisture and temperature. These fungi are aerobic and microscopic and, moreover, may colonize many kinds of food from the field to the table. Mycotoxins are not only a spoilage issue for food, but in high doses can be a serious health threat for humans. The book will be similar to Weidenborner's previous two books - "Mycotoxins in Feedstuffs" and "Mycotoxins in Foodstuffs" - in that it will be a review of the literature to create a comprehensive reference for mycotoxin levels. It will be his third (and last) book on the topic, this time focusing on the incidence of a mycotoxin in humans and/or animals (natural or artificial incidence). Each entry will include contamination, concentration rate, mean concentration of organs (humans and animals) with a mycotoxin, as well as sample constitution (where possible) and country of origin of the sample.

The purpose of this third edition of Developmental and Reproductive Toxicology is to provide a practical guide to developmental and reproductive toxicology in a regulatory environment. In addition to a comprehensive update of current chapters, the third edition been revised to reflect recent changes in the field. It contains new chapters that reflect emerging topics of interest, including testing of biologics (including vaccines), nonhuman primates as nonclinical models, developmental immunotoxicity testing, in vitro assays (such as use of zebrafish and stem cells, as well as high throughput screening), in silico systems modelling, evaluating mechanisms of reproductive toxicity, in-depth coverage of neurobehavioral testing, and testing under the EU's REACH regulations, as well as updated chapters on nonclinical juvenile toxicity testing, endocrine disruptor screening, and on functional and computational genomics. The study of hazard and risk associated with exposure to toxicants during prenatal development has been expanded in recent years to include effects on development until the time of puberty. Concern over the adverse effects of chemical or physical agents on the reproductive processes of both sexes has increased, and progress has been made in identifying the causes and mechanisms eliciting congenital defects and determining the genetic, epigenetic, and environmental factors involved. This book provides up-to-date guidance on the use and interpretation of the newest research techniques in developmental and reproductive toxicology, as well as the more traditional approaches. Developmental and Reproductive Toxicology, Third Edition: Contains valuable insights gained from hands-on experience, together with a critical evaluation of current testing strategies. Includes guidance for the design, conduct, and interpretation of tests in all areas of developmental and reproductive toxicity. Contains reprinted guidelines from major regulatory agencies, as well as terminology for description of developmental abnormalities in laboratory animals, for easy reference. Provides guidance for planning and conducting preclinical toxicity studies and follow-up studies, and interpreting their results in a regulatory environment. This book continues to be the ideal practical reference for developmental and reproductive toxicologists who perform research in industry, government, and academia and for anyone who intends to enter these research areas.

Adds three new chapters (constructed wetlands, stream sediments, and rice production systems) and is updated throughout Examines the adaptation of wetland plants to varying anaerobic soil conditions Provides the basic concepts involved in biogeochemical cycling of nutrients and contaminants in wetlands as related to environmental significance and ecological functions Examines the role of microbial processes in sulfate reduction, denitrification, and methane production Provides case studies for key biogeochemical processes governing wetland function in freshwater wetlands

Biomonitoring is a vital and rapidly growing field. Freshwater Biomonitoring and Benthic Macroinvertebrates presents a state-of-the-art look at the use of benthic macroinvertebrates (aquatic insects, molluscs, crustaceans, and worms) in the biological assessment of water quality in lakes and streams. The use of these organisms has increased dramatically in the past two decades in both North America and Europe; no other comprehensive overview of the topic is available. This book contains contributions from nineteen experts from North America, Europe, and Australia. Some chapters contain updates and syntheses of new information on previously reviewed topics, while others present the first detailed coverage of a topic. The book includes much useful reference material on the history of biomonitoring with invertebrates as well as work of a more practical nature. The design, implementation, analysis and interpretation of benthic surveys are discussed in detail, as are toxicity testing and field experiments. This timely and important book will be of interest to professional entomologists, invertebrate zoologists, and aquatic ecologists, especially those in applied areas concerned with environmental quality, preservation, and restoration. Those working in the growing environmental biotechnology field, or in government agencies responsible for monitoring drinking water, environmental quality, or marine habitats, will also find many new ideas here.

International concern in scientific, industrial, and governmental communi ties over traces of xenobiotics in foods and in both abiotic and biotic envi ronments has justified the present triumvirate of specialized publications in this field: comprehensive reviews, rapidly published research papers and progress reports, and archival documentations. These three international publications are integrated and scheduled to provide the coherency essential for nonduplicative and current progress in a field as dynamic and complex as environmental contamination and toxicology. This series is reserved ex clusively for the diversified literature on "toxic" chemicals in our food, our feeds, our homes, recreational and working surroundings, our domestic animals, our wildlife and ourselves. Tremendous efforts worldwide have been mobilized to evaluate the nature, presence, magnitude, fate, and toxi cology of the chemicals loosed upon the earth. Among the sequelae of this broad new emphasis is an undeniable need for an articulated set of authoritative publications, where one can find the latest important world literature produced by these emerging areas of science together with docu mentation of pertinent ancillary legislation. Research directors and legislative or administrative advisers do not have the time to scan the escalating number of technical publications that may contain articles important to current responsibility. Rather, these individu als need the background provided by detailed reviews and the assurance that the latest information is made available to them, all with minimal literature searching."

Mercury is widespread in our environment. Methylmercury, an organic form of mercury, can accumulate in the aquatic food chain and lead to high concentrations in predatory fish. When consumed by humans, contaminated fish represent a public health risk. Toxic Effects of Mercury intends to facilitate among its readers the understanding of the importance of mercury pollution in the environment and the health consequences associated with exposure to this metal. The knowledge on methylmercury (MeHg) toxicity collected over the years is undoubtedly robust creating an impression all that is to be learnt about this metal has already been accomplished. However, in large measure, past knowledge has merely laid the ground for interesting questions that have yet to be fully addressed and concepts have yet to be deciphered. One of my major goals was to make a valiant attempt to include state-of-the-art information on the mechanisms of mercury toxicity, describing its effects on cultured cellular systems as well as in whole living organisms, starting from the lessons learned from the tragic events in Minamata Bay, Japan. A special focus of the book is on the neurotoxic effects of MeHg. An understanding at the cellular level is necessary to gather information on the structural and functional alterations induced by MeHg and how they possibly become unmasked and evident at the behavioral level, 32 chapters of the book have been organisedhaving these considerations in mind.

This book will provide state-of-the-art information to the graduate students training in toxicology, risk assessors, researchers and medical providers at large. It is aimed to bring the readers updated information on contemporary issues associated with exposure to methylmercury, from its effects on stem cells and neurons to population studies. It is a valuable resource for individuals interested in the public health effects and regulation of mercury. The report provides an excellent example of the implications of decisions in the risk assessment process for a larger audience and is written with the hope that the information will provide better understanding of the mercury problems which confront us."

The job of the responsible zoologist should be to assess or attempt to predict the consequences of any effluent or other environmental disturbance as objectively as possible, bearing in mind both the needs of conservation and the reasonable demands of man. This book has been written to assist this process of assessment and prediction as far as thermal discharges are concerned. It gives a summary and review of the biological studies which have been carried out in various habitats and in response to a variety of problems related to cooling water usage, particularly on the large scale as in thermal power stations. By discussing and summarizing the hugely variable field studies, the book should help the reader to unravel the complexity of the effects of at least one kind of effluent. It should also be of use in the future planning of industries which use cooling water and for the controlling authorities whose task is to set realistic constraints on these industries.

Biodiversity is the delicate ecological balance within biological systems such as species and populations. Evidence suggests air pollution disrupts and impoverishes ecosysytems processes, and genetic and population diversity. Based on a symposium conducted by the EPA's Environmental Research Laboratory, this book pulls together current knowledge on the subject, assesses its relevance, and offers a framework for future research on the impact of air pollution on biodiversity through all levels of biological organization. This text is particularly timely due to acid rain and other toxic problems. The text also discusses the best available control technology, management practices, alternative chemicals, and legislative ways to reduce the impact of air pollution on biodiversity.

Concern over the effects of chemicals in the environment has been increasing for many years. Environmental contamination by DDT, Aldrin, Dieldrin, mercury, PCBs, organotins and many other sub stances are all part of the public consciousness and have led to widespread attention to this topic. Some of the concerns have arisen because human health has been affected when contaminants have been consumed via the food chain-for instance in the case of 'Minimata disease' in Japan. In other cases, direct effects on other components of ecosystems have given cause for alarm. The toxic effects which any chemical can cause are a function of exposure and innate toxicity, i.e. of the ability to reach in sufficient quantity a site where a biological process can be disrupted and of the tendency to cause disruption when it gets there. The processes by which chemicals reach sites of toxic action are the subject of this book, and are a fundamental consideration in ecotoxi cology. When a chemical enters the environment e.g. via a spillage or in an effluent, it is potentially subject to a wide variety of processes which may eliminate it from the environment completely, modify it into a more or less harmful substance, or transfer it to another part of the environment. The processes involved are complex and highly variable, but it is essential to increase our understanding of them."

Reviews of Environmental Contamination and Toxicology attempts to provide concise, critical reviews of timely advances, philosophy and significant areas of accomplished or needed endeavor in the total field of xenobiotics, in any segment of the environment, as well as toxicological implications.

Bacteria in a test tube and humans on our planet follow the same population curve as time elapses. We on this earth, largely through control of disease, are rapidly ap proaching maximum population, and through energy demands are using up our resour ces and accumulating wastes. We have come to realize the danger of a maturing system but have made essential ly no impact in mitigating or preventing the problems we face. While we have been able to change the world we live in, most of our effects have been to degrade the planet. We have nibbled away at the quality of the atmosphere for centuries and the ac cumulated damage has resulted in changing the world's climate at an ever-increasing rate. We now face the possibility of higher average temperatures over most of our planet, changing patterns of rainfall, rising sea levels, aggravated storm systems and a myriad of social effects.

single toxicant before it, yet one that has now been brought under effective control-at least in estuaries and the nearshore environment. The problem with TBT and its cause was first recognized in France, then in the United Kingdom and the United States of America; and in these and other countries legislation is now in place (see Abel, Chapter 2; Champ and Wade, Chapter 3), but in many countries the hazard is only now being identified. This volume has the important function of making available to all a summary of the results of work on TBT and the main conclusions. It will help to minimize the duplication of research and speed the introduction of legislation around the world to control organotin pollution. It is the more valuable because research on TBT has often been published in less accessible journals and symposium proceedings. This volume brings together accounts of these findings by the major contributors to the TBT story, providing the most comprehensive account to date. The TBT problem has proved to be instructive in a number of different ways beyond the bounds of the specific issue (Stebbing, 1985). Most important is that TBT can be seen as a challenge to monitoring systems for nearshore waters, by which it can be judged how effective monitoring has been in fulfilling its purpose, and what improvements should be made. Most instructive was the time it took to bring TBT under control.

Whilst heavy metal phytotoxicity has been known for more than a century, it is astonishing that interest in the effects of heavy metals on organisms has only recently received added attention. Research in the past years, however, has confirmed the immense damage by metal pollution to plants, the soil and ultimately to humans. This completely updated and enlarged second edition gives a state-of-the art review on both field and laboratory work. It deals with the various functional and ecological aspects of heavy metal stress on plants and outlines the scope for future research and the possibilities for remediation.

This authoritative volume examines immunohistochemical methods aimed at investigating the toxicologic pathology of rodent, non-human primate and aquatic animal tissues. Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with a comprehensive review of the methods and approaches for immunohistochemical staining in various target tissues. It explores the tissue-antigen and antibody-specific problems that may be encountered during the staining procedures and provide potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents, monkeys and aquatic animal tissues.