DNA replication is arguably the most crucial process at work in living cells. It is the mechanism by which organisms pass their genetic information from one generation to the next and life on Earth would be unthinkable without it. Despite the discovery of DNA structure in the 1950s, the mechanism of its replication remains rather elusive. This work makes important contributions to this line of research. In particular, it addresses two key questions in the area of DNA replication: which evolutionary forces drive the positioning of replication origins in the chromosome and how is the spatial organization of replication factories achieved inside the nucleus of a cell?. A cross-disciplinary approach uniting physics and biology is at the heart of this research. Along with experimental support, statistical physics theory produces optimal origin positions and provides a model for replication fork assembly in yeast. Advances made here can potentially further our understanding of disease mechanisms such as the abnormal replication in cancer.

In early April 1911 Albert Einstein arrived in Prague to become full professor of theoretical physics at the German part of Charles University. It was there, for the first time, that he concentrated primarily on the problem of gravitation. Before he left Prague in July 1912 he had submitted the paper Relativitat und Gravitation: Erwiderung auf eine Bemerkung von M. Abraham in which he remarkably anticipated what a future theory of gravity should look like.

At the occasion of the Einstein-in-Prague centenary an international meeting was organized under a title inspired by Einstein's last paper from the Prague period: "Relativity and Gravitation, 100 Years after Einstein in Prague." The main topics of the conference included: classical relativity, numerical relativity, relativistic astrophysics and cosmology, quantum gravity, experimental aspects of gravitation and conceptual and historical issues.

The conference attracted over 200 scientists from 31 countries, among them a number of leading experts in the field of general relativity and its applications. This volume includes abstracts of the plenary talks and full texts of contributed talks and articles based on the posters presented at the conference. These describe primarily original results of the authors. Full texts of the plenary talks are included in the volume "General Relativity, Cosmology and Astrophysics--Perspectives 100 Years after Einstein in Prague," eds. J. Bi ak and T. Ledvinka, published also by Springer Verlag."

The best way to become acquainted with a subject is to write a book about it. BenjaminDisraeli Cryobiologyisatruemultidisciplinaryscienceinvolvingconceptsfrombiology, medicine, and physics. Its ?eld comprises the study of any biologicalobject or system (e. g. , proteins, cells, tissues, organs, or organisms) under the temp- atures below the normal (ranging from hypothermic conditions to cryogenic temperatures): cold-adaptation of organisms; cryoconservation of biological objects; conservation of organs under hypothermic conditions; lyophilization; cryosurgery. Origins of cryobiology could be traced down to ancient Eg- tians; probably the ?rst scienti?c account of this science is the monograph by Sir Robert Boyle "New Experiments and Observations Touching Cold" (London, 1683). Twentieth century witnessed a rapid development of cryo- ologyrelatedtotheprogressofthecryogenicequipment(closedsystemsbased on liquid nitrogen, Joule-Tohomson cooling with mixed gases, etc. ), devel- ments of monitoring techniques, extension of the list of diseases that have been successfully treated by cryomedicine, and consolidation of research by foundation (simultaneously in 1964) of two major scienti?c societies in this ? eld - The Society for Cryobiology and The Society for Low Temperature Biology. There are a lot of good books on cryobiologythat can be divided into two groups: (1) the ones that treat the whole ?eld of cryobiology - these ones are somewhatout-of-dateand(2)thebooksonspeci?capplicationsofcryobiology such as cryosurgery or cryoconservation.

Here, the authors present modern mathematical methods to solve problems of differential-operator inclusions and evolution variation inequalities which may occur in fields such as geophysics, aerohydrodynamics, or fluid dynamics. For the first time, they describe the detailed generalization of various approaches to the analysis of fundamentally nonlinear models and provide a toolbox of mathematical equations. These new mathematical methods can be applied to a broad spectrum of problems. Examples of these are phase changes, diffusion of electromagnetic, acoustic, vibro-, hydro- and seismoacoustic waves, or quantum mechanical effects. This is the second of two volumes dealing with the subject.

This book takes the reader for a short journey over the structures of matter showing that their main properties can be obtained even at a quantitative level with a minimum background knowledge including, besides first year calculus and physics, the extensive use of dimensional analysis and the three cornerstones of science, namely the atomic idea, the wave-particle duality and the minimization of energy as the condition for equilibrium. Dimensional analysis employing the universal physical constants and combined with "a little imagination and thinking", to quote Feynman, allow an amazing short-cut derivation of several quantitative results concerning the structures of matter. In the current 2nd edition, new material and more explanations with more detailed derivations were added to make the book more student-friendly. Many multiple-choice questions with the correct answers at the end of the book, solved and unsolved problems make the book also suitable as a textbook. This book is of interest to students of physics, engineering and other science and to researchers in physics, material science, chemistry and engineering who may find stimulating the alternative derivation of several real world results which sometimes seem to pop out the magician's hat.

Proceedings from the 2012 Fourth International Meeting on Gravitation and Cosmology, focusing on accelerated cosmic expansion

This volume provides both an update and a review of the state of alternative theories of gravity in connection with the accelerated expansion of the universe issue. Different theoretical proposals exist to explain the acceleration in the cosmic expansion, generating the dark energy issue and opening the possibility to theories of gravity alternative to general relativity. Related issues such as the dark matter problem are also surveyed in order to give the readers profound insight on the subject from different points of view. Comprised of short talks and plenary lectures given by leading experts in the field, some of them with brilliant and historic contributions, the book allows the reader to find readable and referenced surveys in topics like f(R) theories, the dark matter and dark energy issues, Modified Newtonian Dynamics (MOND) scenarios, f(T) theories, scalar-tensor theories derived from non-Riemannian geometries, emergent universes, the cosmological constant and other topics of current interest for younger and senior physicists and graduate students.

These proceedings are from the Fourth International Meeting on Gravitation and Cosmology, held in Guadalajara, Jalisco, Mexico, from 20 - 25 May, 2012, was sponsored by ICTP- Trieste, Italy and COECyTJAL-Universidad de Guadalajara, Mexico. This event is a series of scientific meetings started in 2004 in Cuba, focusing on current and selected topics in the fields of gravitation and cosmology."

The goal of the project presented in this book is to detect neutrinos created by resonant interactions of ultrahigh energy cosmic rays on the CMB photon field filling the Universe. In this pioneering first analysis, the author puts forward much of the analysis framework, including calibrations of the electronic hardware and antenna geometry, as well as the development of algorithms for event reconstruction and data reduction. While only two of the 37 stations planned for the Askaryan Radio Array were used in this assessment of 10 months of data, the analysis was able to exclude neutrino fluxes above 10 PeV with a limit not far from the best current limit set by the IceCube detector, a result which establishes the radio detection technique as the path forward to achieving the massive volumes needed to detect these ultrahigh energy neutrinos.

In recent years, the increased availability and fidelity of broadband seismic instruments have effectively narrowed the gap between exploration and global seismic applications. Global seismologists are now able to take advantage of high-resolution, often exploration-based, tools to examine rock properties tens to hundreds of kilometers below surface. This book reviews the key assumptions, algorithms and prospects of several important array-based methods in today s global and regional seismic surveys. A short list of topics includes data migration, PP and SS precursors, Radon transform, mantle triplication, P-to-S and S-to-P converted waves, shear-wave splitting, high-resolution seismic tomography, and ambient-noise interferometry. Each approach is presented in a cookbook fashion for easy comparison, implementation and critique by the general readership."

It is now well established that all living systems emit a weak but permanent photon flux in the visible and ultraviolet range. This biophoton emission is correlated with many, if not all, biological and physiological functions. There are indications of a hitherto-overlooked information channel within the living system. Biophotons may trigger chemical reactivity in cells, growth control, differentiation and intercellular communication, i.e. biological rhythms. The basic experimental and theoretical framework as well as the technical problems and the wide field of applications in the biotechnical, biomedical engineering, engineering, medicine, pharmacology, environmental science and basic science fields are presented in this book. To promote the dialog and mutual penetration between biophoton research and photon technology is one of the important goals for the International Conference on Biophotons & Biophotonics 2003, and is developed and presented in Biophotonics: Optical Science and Engineering in the 21st Century.

Computational Biomechanics for Medicine: Solid and fluid mechanics for the benefit of patients contributions and papers from the MICCAI Computational Biomechanics for Medicine Workshop help in conjunction with Medical Image Computing and Computer Assisted Intervention conference (MICCAI 2019) in Shenzhen, China. The content is dedicated to research in the field of methods and applications of computational biomechanics to medical image analysis, image-guided surgery, surgical simulation, surgical intervention planning, disease prognosis and diagnostics, analysis of injury mechanisms, implant and prostheses design, as well as artificial organ design and medical robotics. These proceedings appeal to researchers, students and professionals in the field.

This book presents a systematic attempt to generalize several fundamental physical laws related to subsurface fluid flow that are important for a number of contemporary applications in the areas of hydrogeology, reservoir engineering and rock mechanics. It also covers the history of discovering these physical laws, their respective scope of validity, and their generalizations or extensions. The physical laws discussed include Darcy's law, Darcy-Buckingham law and Hooke's law. Darcy's law is the fundamental law for subsurface fluid flow. For low-permeability media, it is not always adequate because of the strong fluid-solid interaction. Though the Darcy-Buckingham law is often used for modeling subsurface multiphase flow, it is only valid under the local equilibrium condition. This condition does not hold in many cases, especially when fingering flow occurs. It is well known that subsurface fluid flow is coupled with mechanical deformation of subsurface media; in some applications, this coupling can play a dominant role. The continuum-scale elastic deformation of natural rock, however, does not always follow the traditional form of Hooke's law. The book also presents applications of the proposed generalizations of the physical laws to several important engineering projects.

Fluorescent proteins are intimately connected to research in the life sciences. Tagging of gene products with fluorescent proteins has revolutionized all areas of biosciences, ranging from fundamental biochemistry to clinical oncology, to environmental research. The discovery of the Green Fluorescent Protein, its first, seminal application and the ingenious development of a broad palette of fluorescence proteins of other colours, was consequently recognised with the Nobel Prize for Chemistry in 2008.

"Fluorescent Proteins I" is devoted to the basic photophysical and photochemical aspects of fluorescent protein technology. Experienced experts highlight colour tuning, the exploration of switching phenomena and respective methods for their investigation. The book provides a thorough understanding of primary molecular processes allowing the design of fluorescent proteins for specific applications.

The Spectroscopy of H₃⁺ (I. McNab).

Supercooled Liquids (U. Mohanty).

Ternary Systems Containing Surfactants (M. Laradji, et al.).

Colored Noise in Dynamical Systems (P. Hänggi & P. Jung).

Formulation of Oscillatory Reaction Mechanisms by Deduction from Experiments (J. Stemwedel, et al.).

Indexes.

Tsunamis are long water waves generated by impulsive geophysical motions of the seafloor. They inflict significant damage and casualties both near-field and after evolving over long propagation distances and impacting distant coastlines. They can also affect geomorphologic changes along the coast. Tsunamis can be triggered by sea floor deformation, landslides, slumps, subsidence, volcanic eruptions and bolide impacts. Understanding tsunami generation is of paramount importance for protecting coastal populations at risk, coastal structures and the natural environment. generation, so that adequate discrimination of their sources from coastal inundation data is difficult. The accurate and reliable prediction of the initial waveform and the associated coastal effects of tsunamis remains one of the most vexing problems in geophysics, and - with few exceptions - has resisted routine numerical computation or off-the-shelf solutions. with contributions ranging from basic and applied science to coastal zone management. It is aimed at tsunami scientists, coastal and ocean engineers, marine geologists and geophysicists, planners and policy makers, and coastal zone managers seeking to better understand and mitigate the coastal impact of tsunamis.

The space between the stars contains a large diversity of objects in which physical processes occur that are fundamental to the structure and evolution of galaxies. This book offers the reader a basic knowledge of these processes and presents simple numeric estimates of the main quantities relevant to the interstellar medium. The main objects that constitute the interstellar space are described, but the emphasis of the book lies in the physical processes occurring in these objects, which may also occur in other astrophysical environments. The book is directed tor graduate as well as advanced undergraduate students of physics and astrophysics.

The years 2012/2013 mark the 50th anniversary of the theoretical prediction that Brown Dwarfs, i.e. degenerate objects which are just not massive enough to sustain stable hydrogen fusion, exist. Some 20 years after their discovery, how Brown Dwarfs form is still one of the main open questions in the theory of star formation. In this volume, the pioneers of Brown Dwarf research review the history of the theoretical prediction and the subsequent discovery of Brown Dwarfs. After an introduction, written by Viki Joergens, reviewing Shiv Kumar's theoretical prediction of the existence of brown dwarfs, Takenori Nakano reviews his and Hayashi's calculation of the Hydrogen Burning Minimum Mass. Both predictions happened in the early 1960s. Jill Tarter then writes on the introduction of the term 'Brown Dwarf', before Ben Oppenheimer, Rafael Rebolo and Gibor Basri describe their first discovery of Brown Dwarfs in the 1990s. Lastly, Michael Cushing and Isabelle Baraffe describe the development of the field to the current state of the art. While the book is mainly aimed at the Brown Dwarf research community, the description of the pioneering period in a scientific field will attract general readers interested in astronomy as well.

Disulfide-containing proteins belong to a unique class of proteins for studying the mechanism of protein folding. Their folding mechanism can be analyzed by three distinct techniques: (1) The conventional denaturation-renaturation method (disulfide intact); (2) The disulfide oxidation method (oxidative folding); and (3) The emerging disulfide scrambling method. Each technique provides specific information as to how an unfolded disulfide protein refolds to form the native structure. This book is intended to highlight the knowledge of several important proteins (BPTI, RNase A, beta-Lactalbumin and Lysozyme etc.) that have been characterized in depth by these methodologies. The book will also devote sections to comparing these methodologies and chaperones (PDI and Dsb machineries) that facilitate folding of disulfide proteins. Folding of Disulfide Proteins aims to cover the knowledge of protein folding accumulated from studies of disulfide-containing proteins, including methodologies, folding pathways, and folding mechanism of numerous extensively characterized disulfide proteins. This book will be of interest to those interested in problems related to protein folding, and anyone who is interested in understanding the mechanism of protein misfolding and protein misfolding-related diseases. Folding of Disulfide Proteins aims to cover the knowledge of protein folding accumulated from studies of disulfide-containing proteins, including methodologies, folding pathways, and folding mechanism of numerous extensively characterized disulfide proteins. This book will be of interest to those interested in problems related to protein folding, and anyone who is interested in understanding the mechanism of protein misfolding and protein misfolding-related diseases.

Several of the very foundations of the cosmological standard model the baryon asymmetry of the universe, dark matter, and the origin of the hot big bang itself still call for an explanation from the perspective of fundamental physics. This workadvocates one intriguing possibility for a consistent cosmology that fills in the theoretical gaps while being fully in accordance with the observational data. At very high energies, the universe might have been in a false vacuum state that preserved B-L, the difference between the baryon number B and the lepton number L as a local symmetry. In this state, the universe experienced a stage of hybrid inflation that only ended when the false vacuum became unstable and decayed, in the course of a waterfall transition, into a phase with spontaneously broken B-L symmetry. This B-L Phase Transition was accompanied by tachyonic preheating that transferred almost the entire energy of the false vacuum into a gas of B-L Higgs bosons, which in turn decayed into heavy Majorana neutrinos. Eventually, these neutrinos decayed into massless radiation, thereby producing the entropy of the hot big bang, generating the baryon asymmetry of the universe via the leptogenesis mechanism and setting the stage for the production of dark matter. Next to a variety of conceptual novelties and phenomenological predictions, the main achievement of the thesis is hence the fascinating notion that the leading role in the first act of our universe might have actually been played by neutrinos.
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Starting from Sophus Lie, the invariance of a differential equation under its continuous group of symmetries has become a major tool for solving ordinary and partial differential equations, in particular, nonlinear ones. The proceedings focus on the application of these techniques to nonlinear partial differential equations. The state of the art in this field is presented clearly in a series of comprehensive lectures. Several lectures on applications point out the physical importance of such methods.

This book is an edited version of lectures given by the authors at the 1985 Jilin University Summer School on Laser Physics. The School was held at Jilin University in Changchun, Peoples' Republic of China.

This volume provides a comprehensive selection of recent studies addressing insect hearing and acoustic communication. The variety of signalling behaviours and hearing organs makes insects highly suitable animals for exploring and analysing signal generation and hearing in the context of neural processing, ecology, evolution and genetics. Across a variety of hearing species like moths, crickets, bush-crickets, grasshoppers, cicadas and flies, the leading researchers in the field cover recent scientific progress and address key points in current research, such as:

. How can we approach the evolution of hearing in insects and what is the developmental and neural origin of the auditory organs?

. How are hearing and sound production embedded in the natural lifestyle of the animals, allowing intraspecific communication but also predator avoidance and even predation?

. What are the functional properties of hearing organs and how are they achieved at the molecular, biophysical and neural levels?

. What are the neural mechanisms of central auditory processing and signal generation?

The book is intended for students and researchers both inside and outside of the fascinating field of bioacoustics and aims to foster understanding of hearing and acoustic communication in insects."

The Earth's average temperature has risen by 1.4 DegreesF over the past century, and computer models project that it will rise much more over the next hundred years, with significant impacts on weather, climate, and human society. Many climate scientists attribute these increases to the build up of greenhouse gases produced by the burning of fossil fuels and to the anthropogenic production of short-lived climate pollutants. Climate Change Modeling Methodologies: Selected Entries from the Encyclopaedia of Sustainability Science and Technology provides readers with an introduction to the tools and analysis techniques used by climate change scientists to interpret the role of these forcing agents on climate. Readers will also gain a deeper understanding of the strengths and weaknesses of these models and how to test and assess them. The contributions include a glossary of key terms and a concise definition of the subject for each topic, as well as recommendations for sources of more detailed information.

Understanding the stars is the bedrock of modern astrophysics. Stars are the source of life. The chemical enrichment of our Milky Way and of the Universe withallelementsheavierthanlithiumoriginatesintheinteriorsofstars.Stars arethe tracersofthe dynamics ofthe Universe,gravitationallyimplying much more than meets the eye. Stars ionize the interstellar medium and re-ionized the early intergalactic medium. Understanding stellar structure and evolution is fundamental. While stellar structure and evolution are understood in general terms, we lack important physical ingredients, despite extensive research during recent decades.Classicalspectroscopy,photometry,astrometryandinterferometryof stars have traditionally been used as observational constraints to deduce the internal stellar physics. Unfortunately, these types of observations only allow the tuning of the basic common physics laws under stellar conditions with relatively poor precision. The situation is even more worrisome for unknown aspects of the physics and dynamics in stars. These are usually dealt with by using parameterised descriptions of, e.g., the treatments of convection, rotation,angularmomentumtransport,theequationofstate,atomicdi?usion andsettlingofelements,magneto-hydrodynamicalprocesses,andmore.There is a dearth of observational constraints on these processes, thus solar values areoftenassignedtothem.Yetitishardtoimaginethatonesetofparameters is appropriate for the vast range of stars.

The volumes in this authoritative series present a multidisciplinary approach to modeling and simulation of flows in the cardiovascular and ventilatory systems, especially multiscale modeling and coupled simulations. Volume 5 is devoted to cells, tissues, and organs of the cardiovascular and ventilatory systems with an emphasis on mechanotransduction-based regulation of flow. The blood vessel wall is a living tissue that quickly reacts to loads applied on it by the flowing blood. In any segment of a blood vessel, the endothelial and smooth muscle cells can sense unusual time variations in small-magnitude wall shear stress and large-amplitude wall stretch generated by abnormal hemodynamic stresses. These cells respond with a short-time scale (from seconds to hours) to adapt the vessel caliber. Since such adaptive cell activities can be described using mathematical models, a key objective of this volume is to identify the mesoscopic agents and nanoscopic mediators required to derive adequate mathematical models. The resulting biomathematical models and corresponding simulation software can be incorporated into platforms developed in virtual physiology for improved understanding and training.