This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitches as targets and tools and contains sections on such topics as constructing and optimizing artificial riboswitches, live cell imaging and intracellular sensors with artificial riboswitches, conditional control of gene expression with artificial riboswitches, using artificial riboswitches for protein evolution and pathway optimization, and anti-riboswitch drug screens.

The Management of Scientific Integrity within Academic Medical Centers discusses the impact scientific misconduct has in eight complex case studies. Authors look at multifaceted mixtures of improper behavior, poor communication, cultural issues, adverse medical/health issues, interpersonal problems and misunderstandings to illustrate the challenge of identifying and managing what went wrong and how current policies have led to the establishment of quasi legal processes within academic institutions. The book reviews the current global regulations and concludes with a section authored by a US federal court judge who provides his perspective on the adequacy of current regulations and policies.

In this issue of Medical Clinics, guest editor Melissa B. Weimer brings her considerable expertise to the topic of Substance Use Disorders. Provides in-depth, clinical reviews on the latest updates in Substance Use Disorders, providing actionable insights for clinical practice. Presents the latest information on this timely, focused topic under the leadership of experienced editors in the field; Authors synthesize and distill the latest research and practice guidelines to create these timely topic-based reviews.

In this issue of Child and Adolescent Psychiatric Clinics, new Consulting Editor and Issue Guest Editor Justine Larson brings her considerable expertise in the latest hot topics in child and adolescent psychiatry. Top experts in the field cover key topics such as bullying, depression intervention, the biological effects of childhood trauma, childhood trauma and psychosis, and more. Provides in-depth, clinical reviews on the latest hot topics in child and adolescent psychiatry, providing actionable insights for clinical practice. Presents the latest information on this timely, focused topic under the leadership of experienced editors in the field; Authors synthesize and distill the latest research and practice guidelines to create these timely topic-based reviews. Contains 15 relevant, practice-oriented topics including Social Media as It Interfaces with Psychosocial Development and Mental Illness in Transitional Age Youth; Mental Health Care of Detained Youth and Solitary Confinement and Restraint Within Juvenile Detention Facilities; Adverse Childhood Experiences, Resilience and Mindfulness-Based Approaches Common Denominator Issues for Children with Emotional, Mental, or Behavioral Problems; #KidsAnxiety and Social Media; and more.

With at least 40% new or updated content since the last edition, "Clinical Decision Support," 2nd Edition explores the crucial new motivating factors poised to accelerate Clinical Decision Support (CDS) adoption. This book is mostly focused on the US perspective because of initiatives driving EHR adoption, the articulation of 'meaningful use', and new policy attention in process including the Office of the National Coordinator for Health Information Technology (ONC) and the Center for Medicare and Medicaid Services (CMS). A few chapters focus on the broader international perspective. "Clinical Decision Support," 2nd Edition explores the technology, sources of knowledge, evolution of successful forms of CDS, and organizational and policy perspectives surrounding CDS.

Exploring a roadmap for CDS, with all its efficacy benefits including reduced errors, improved quality, and cost savings, as well as the still substantial roadblocks needed to be overcome by policy-makers, clinicians, and clinical informatics experts, the field is poised anew on the brink of broad adoption. "Clinical Decision Support," 2nd Edition provides an updated and pragmatic view of the methodological processes and implementation considerations. This book also considers advanced technologies and architectures, standards, and cooperative activities needed on a societal basis for truly large-scale adoption.
At least 40% updated, and seven new chapters since the previous edition, with the new and revised content focused on new opportunities and challenges for clinical decision support at point of care, given changes in science, technology, regulatory policy, and healthcare financeInforms healthcare leaders and planners, health IT system developers, healthcare IT organization leaders and staff, clinical informatics professionals and researchers, and clinicians with an interest in the role of technology in shaping healthcare of the future

The critically acclaimed laboratory standard for almost 50 years, "Methods in Enzymology" is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few.

This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: Pulse-chase analysis to measure protein degradationLabeling a protein with fluorophores using NHS ester derivitizationImmunoaffinity purification of proteinsProteolytic affinity tag cleavagePurification of GST-tagged proteins
Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocolsThis volume focuses on core protocols involving protein

"Medical Care of the Liver Transplant Patient" looks at monitoring and maintaining the health of organ recipients and donors, pre, during and post-operatively.

There are twenty-nine chapters containing practical advice on total patient management. They are arranged into 8 sections and follow the stages of transplantation from first indication and selection of potential recipient, through to acute recovery, long-term follow-up and continued health.

In this edition there are new chapters on special considerations in liver transplant patients such as viral hepatitis, alcoholic liver disease and live donor liver transplantation. It also contains the very latest information concerning complications and recurring problems after transplantation. Another new chapter considers fresh approaches and developments in the future.

This is a vital reference to all members of the medical team involved at different stages in the care of liver transplantation patients including hepatologists, gastroenterologists, transplant surgeons, specialist nurses, and nutritionists.

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Principles of Translational Science in Medicine: From Bench to Bedside, Third Edition, provides an update on major achievements in the translation of research into medically relevant results and therapeutics. The book presents a thorough discussion of biomarkers, early human trials, and networking models, and includes institutional and industrial support systems. It also covers algorithms that have influenced all major areas of biomedical research in recent years, resulting in an increasing number of new chemical/biological entities (NCEs or NBEs) as shown in FDA statistics. New chapters include: Translation in Oncology, Biologicals, and Orphan Drugs. The book is ideal for use as a guide for biomedical scientists to establish a systematic approach to translational medicine and is written by worldwide experts in their respective fields.

In this issue of Medical Clinics, Guest Editor Jeffrey Callen brings his considerable expertise to the topic of Dermatology. Top experts in the field cover key topics such as alopecia, psoriasis, Cutaneous manifestations of diabetes, Diagnosis and management of cellulitis, and more. Provides in-depth, clinical reviews in Dermatology, providing actionable insights for clinical practice. Presents the latest information on this timely, focused topic under the leadership of experienced editors in the field; Authors synthesize and distill the latest research and practice guidelines to create these timely topic-based reviews. Contains more than 10 relevant, practice-oriented topics including Recognition and management of Severe Cutaneous Adverse Drug Reactions (including DRESS, SJS and TEN); Diagnosis and management of eczemas including atopic, hand dermatitis and contact dermatitis; Recognition, staging and management of melanoma; Diagnosis and management of leg ulcers; Approach to the patient with pruritus; and more.

The third edition of this innovative work again provides a unique perspective on the clinical discovery process by providing input from experts within the NIH on the principles and practice of clinical research. Molecular medicine, genomics, and proteomics have opened vast opportunities for translation of basic science observations to the bedside through clinical research. As an introductory reference it gives clinical investigators in all fields an awareness of the tools required to ensure research protocols are well designed and comply with the rigorous regulatory requirements necessary to maximize the safety of research subjects. Complete with sections on the history of clinical research and ethics, copious figures and charts, and sample documents it serves as an excellent companion text for any course on clinical research and as a must-have reference for seasoned researchers.
Incorporates new chapters on Managing Conflicts of Interest in Human Subjects Research, Clinical Research from the Patient's Perspective, The Clinical Researcher and the Media, Data Management in Clinical Research, Evaluation of a Protocol Budget, Clinical Research from the Industry Perspective, and Genetics in Clinical ResearchAddresses the vast opportunities for translation of basic science observations to the bedside through clinical researchDelves into data management and addresses how to collect data and use it for discoveryContains valuable, up-to-date information on how to obtain funding from the federal government

Dental defects may be the physical expression of genetic defects, and so they can often be seen in a variety of syndromes associated with malformations of organs. However, dental defects are often not recognized, identified, nor characterised despite representing a possible diagnostic sign for an undiagnosed condition. This book addresses this gap by providing an understanding of dental genetics and its developmental biology counterpart. With approximately seventy well-illustrated examples, the authors present the clinical oro-facial manifestations accompanying various syndromes, providing the necessary knowledge for diagnostic purposes, as well as giving insight into recent development for each specific condition. The clarity and format of this book make it an ideal support guide both in the clinic and while conducting research.

This book explores serious diseases and disorders that most readers have never heard of, ranging from genetic, infectious, and environmental diseases to autoimmune, idiopathic, and mental disorders. Despite centuries of scientific study and medical research, there are still many human diseases and disorders that remain difficult to manage or are incurable. Some of these maladies are extremely rare, yet, together, they affect a substantial number of people. The 101 Most Unusual Diseases and Disorders examines seldom-seen illnesses, providing high school and college students with an excellent resource for research as well as supplying fascinating reading for general readers interested in diseases and medical science. This book provides clear, easy-to-understand, and scientifically grounded information on the vast number of unusual medical conditions that have been recorded, covering five kinds of diseases and disorders: genetic, infectious, environmental, mental, and "other," which constitutes diseases of autoimmune and unknown origin. Examples of the medical conditions addressed include autoimmune encephalitis, Ebola, kleptomania, Morgellons syndrome, orthorexia, pneumoconiosis, and Prader-Willi syndrome. Selected case studies enable readers to better empathize with the experiences of those who have these disorders and how these afflictions have affected their lives. Provides readers with interesting facts and insights into unusual or rarely seen diseases and disorders from the perspective of an experienced medical writer Supplies accessible information for students and general readers and serves as a jumping-off point for further research into an unusual topic or disorder Addresses a topic of high interest due to recent media attention on deadly contagious diseases such as Ebola as well as popular television shows that focus on uncommon and interesting medical conditions like House and Mystery Diagnosis Includes case studies that serve to "personalize" the content by highlighting the experiences of individuals affected by a particular disease

Produced by microbes on a large scale, methane is an important alternative fuel as well as a potent greenhouse gas. This volume focuses on microbial methane metabolism, which is central to the global carbon cycle. Both methanotrophy and methanogenesis are covered in detail. Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements. The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field.

Topics include isolation and classification of microorganisms, metagenomics approaches, biochemistry of key metabolic enzymes, gene regulation and genetic systems, and field measurements The state-of-the-art techniques described here will both guide researchers in specific pursuits and educate the wider scientific community about this exciting and rapidly developing field