Selecting illustrative examples from the recent literature, this reference studies the underlying principles and physics of a wide range of spectroscopic techniques utilized in the pharmaceutical sciences and demonstrates various applications for each method analyzed in the text-showing how knowledge of the mechanisms of spectroscopic phenomena may facilitate more advanced technologies in the field.

The development of chiral liquid chromatography, facilitating the straightforward separation of enantiomers, was a significant advance in chromatography, leading to widespread application in analytical chemistry. Application in preparative chromatography has been less rapid, but with the development of single enantiomer pharmaceuticals its use is increasingly common in chemical synthesis at laboratory, pilot plant and even full production scale.

Written for chemists, chemical engineers and analytical chemists, Preparative Enantioselective Chromatography demonstrates the considerable utility of the technique in contemporary discovery, development and production scale chemistry. By briefly covering basic preparative chromatography then developing the discussion to cover chiral stationary phases for preparative use, method development and practical applications, the first part of this book serves as an ideal introduction to the technology for laboratory and pilot plant scale application. The more detailed information presented in the later chapters, on applications in discovery, process development and large scale or production environments, including case studies and equipment selection issues, ensures the book will serve as a sound reference for experienced separation scientists.

Storage and conversion are critical components of important energy-related technologies. "Advanced Batteries: Materials Science Aspects" employs materials science concepts and tools to describe the critical features that control the behavior of advanced electrochemical storage systems. This volume focuses on the basic phenomena that determine the properties of the components, i.e. electrodes and electrolytes, of advanced systems, as well as experimental methods used to study their critical parameters. This unique materials science approach utilizes concepts and methodologies different from those typical in electrochemical texts, offering a fresh, fundamental and tutorial perspective of advanced battery systems. Graduate students, scientists and engineers interested in electrochemical energy storage and conversion will find "Advanced Batteries: Materials Science Aspects" a valuable reference.

The last few years have seen an unprecedented drive toward the application of proteomics to resolving challenging biomedical and biochemical tasks. Separation techniques combined with modern mass spectrometry are playing a central role in this drive. This book discusses the increasingly important role of mass spectrometry in proteomic research, and emphasizes recent advances in the existing technology and describes the advantages and pitfalls as well.
* Provides a scientifically valid method for analyzing the approximatey 500,000 proteins that are encoded in the human genome
* Explains the hows and whys of using mass spectrometry in proteomic analysis
* Brings together the latest approaches combining separation techniques and mass spectrometry and their application in proteome analysis
* Comments on future challenges and how they may be addressed
* Includes sections on troubleshooting

Nuclear magnetic resonance (NMR) is an analytical tool used by chemists and physicists to study the structure and dynamics of molecules. In recent years, no other technique has grown to such importance as NMR spectroscopy. It is used in all branches of science where precise structural determination is required and where the nature of interactions and reactions in solution is being studied. Annual Reports on NMR has established itself as a premier means for the specialist and nonspecialist alike to become familiar with new techniques and applications of NMR spectroscopy.
* Includes comprehensive review articles on NMR Spectroscopy
* Provides nonspecialists using this technology in all branches of science with the latest developments in the field
* Keeps specialists up-to-date on the newest techniques in this important and fast-changing field

The first edition of "Chromatography: Concepts and Contrasts," published in 1988, was one of the first books to discuss all the different types of chromatography under one cover. The second edition continues with these principles but has been updated to include new chapters on sampling and sample preparation, capillary electrophoresis and capillary electrochromatography (CEC), chromatography with mass spec detection, and industrial and governmental practices in regulated industries.

• Covers extraction, solid phase extraction (SPE), and solid phase microextraction (SPME), and introduces mass spectrometry
• Updated with the latest techniques in chromatography
• Discusses both liquid chromatography (LC)and gas chromatography(GC)

This volume is intended to show beginners in modern Fourier Transform-Infrared analysis which technique of infrared analysis should be selected and how to use it to obtain certain information from the most common samples brought into research and analytical laboratories in production industries.

This book provides a balanced blend of fundamental concepts of fabrication, characterization of conventional ceramics, extending to present the recent advances in ceramic membranes. It covers the basic concepts of ceramic membranes as well as practical and theoretical knowledge in conventional and advanced ceramic membranes combined with unorthodox ideas for novel approaches in ceramic membranes. Book includes lot of real time examples derived largely from research work by authors. Aimed at researchers, students and academics in the field of membrane engineering around the globe, it has following key features: Guides readers through manufacturing, characterizing and using low-cost ceramic technology. Provides an overview of the different types of ceramic membranes, catalytic reactors and their uses. Covers industrial application, separation and purification. Includes recent developments and advances in membrane fabrication. Discusses new raw materials for ceramic membranes.

Focusing on what has been one of the driving forces behind the development of lab-on-a-chip devices, Separation Methods in Microanalytical Systems explores the implementation, realization, and operation of separation techniques and related complex workflows on microfabricated devices. The book details the design, manufacture, and integration of diverse components needed to perform an entire analytical procedure on a single miniaturized device. The content applies to a diversity of disciplines including chemical analysis, biomedical diagnostics, environmental monitoring, and drug discovery. Separation Methods in Microanalytical Systems lays its theoretical background in a way that scientists from varied disciplines can approach. The book describes factors that influence the performance of separation, such as microfluidic handling, sample pre-treatment, and detection. It also conveys fabrication and material issues, design challenges, and practical considerations. Several chapters describe specific separation techniques that are central to micro-Total Analysis Systems (-TAS) as well as novel methods and emerging trends in microchip-based separations. The book also provides an applications overview that supplies a wealth of examples that help scientists put their ideas in perspective with already existing solutions. This multi-authored volume offers different styles, approaches, and opinions for a given problem, reflecting the various angles researchers take to handle the same issues. A one-stop guide for understanding, designing, and working with separation techniques in microanalytical devices, Separation Methods in Microanalytical Systems is a valuable reference for scientists and engineers already preparing to meet the anticipated demand for function-specific chemical separation systems.

Conjugated polymeric materials and their nanocomposites are widely used for the creation of alternative sources of renewable energy, cell phone screens, mobile gadgets, video players and OLED-TV, as well as organic diodes, transistors, sensors, etc. with field-dependent and spin-assisted electronic properties. Multifrequency EPR Spectroscopy methods can help researchers optimize their structural, magnetic and electronic properties for the creation of more efficient molecular devices. This book will acquaint the reader with the basic properties of conjugated polymers, the fundamentals of EPR Spectroscopy, and the information that can be obtained at different wavebands of EPR spectroscopy.

Metabolomics is a fast growing field in systems biology and offers a powerful and promising approach for a large range of applications. Metabolomics focuses on deriving the concentrations and fluxes of low molecular weight metabolites in bio-fluids, cells or tissue, plants, foods and related samples and this information provides enormous detail on biological systems and their current status. "Mass Spectrometry in Metabolomics: Methods and Protocols "presents a broad coverage of the major mass spectrometry (MS)-based metabolomics methods and applications. MS is one of most powerful and commonly used analytical methods in metabolomics; because so many different MS systems are used in metabolomics, this volume includes a wide variety such as triple quads, time of flight, Fourier transform ion cyclotron resonance and even simple quadrupole systems. A wide range of studies are described, with samples ranging from blood and urine to tissue and even plants. Written in the successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, "Mass Spectrometry in Metabolomics: Methods and Protocols "seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the dynamic field of metabolomics.

Atomic spectrometry has exciting new bio-analytical horizons open to it, principally through the developments in the capabilities of ICP-MS coupled with the inventiveness of experimentalists. This is reflected in the use of the technique for ion-, capillary electrophoresis-, liquid- and gas-chromatographic separation in biological applications, as reported in this book. Traditional (environmental, semiconductor, geological and clinical) applications are also well represented. In addition, recent and future developments in sample introduction devices, multicollector sector, reaction cells and collision cells instruments, as well as co-existence, divergence and potential convergence of atomic and biomolecular mass spectrometries are discussed. Reflecting the current state of practical ICP-MS and drawing together the latest developments in the field, Plasma Source Mass Spectrometry: Current Trends and Future Developments is ideal for university researchers and laboratory practitioners. It will be of interest to all those involved in the development and application of this technique.

High-Resolution NMR Techniques in Organic Chemistry, Third Edition describes the most important NMR spectroscopy techniques for the structure elucidation of organic molecules and the investigation of their behaviour in solution. Appropriate for advanced undergraduate and graduate students, research chemists and NMR facility managers, this thorough revision covers practical aspects of NMR techniques and instrumentation, data collection, and spectrum interpretation. It describes all major classes of one- and two-dimensional NMR experiments including homonuclear and heteronuclear correlations, the nuclear Overhauser effect, diffusion measurements, and techniques for studying protein-ligand interactions. A trusted authority on this critical expertise, High-Resolution NMR Techniques in Organic Chemistry, Third Edition is an essential resource for every chemist and NMR spectroscopist.

This book reviews advances in important and practically relevant homogeneous catalytic transformations, such as single-site olefin polymerizations and chemo- and stereo-selective oxidations. Close attention is paid to the experimental investigation of the active sites of catalytic oxidation systems and their mechanisms. Major subjects include the applications of NMR and EPR spectroscopic techniques and data obtained by other physical methods. The book addresses a broad readership and focus on widespread techniques available in labs with NMR and EPR spectrometers.

Nuclear magnetic resonance (NMR) is an analytical tool used by chemists and physicists to study the structure and dynamics of molecules. In recent years, no other technique has grown to such importance as NMR spectroscopy. It is used in all branches of science where precise structural determination is required and where the nature of interactions and reactions in solution is being studied.
Annual Reports on NMR has established itself as a premier means for the specialist and nonspecialist alike to become familiar with new techniques and applications of NMR spectroscopy.
* Includes comprehensive review articles on NMR Spectroscopy
* NMR is used in all branches of science
* No other technique has grown to such importance as NMR Spectroscopy in recent years

Unlike traditional NMR textbooks for chemists, this fresh look at the topic combines theory, technology and application in a wide range of fields, targeting biochemists, medicinal chemists, and structural biologists, as well as organic chemists. The text has been developed from a one-semester graduate-level course taught by the authors at the University of Zurich, and offers numerous intuitive illustrations, training exercises and plain-language explanations of complex theory. Divided into four major parts, the first introduces the theory, providing a profound understanding of why experiments work, without a rigorous mathematical treatment of all the physico-chemical computations and deliberately shorter than in most other NMR textbooks. Part two discusses current instrumentation and practical aspects, including sample preparation, processing of raw data and the use of databases, while the third part focuses on the main application of NMR, with examples and training spectra taken from a wide range of synthetic and natural compounds. Part four introduces a selection of modern NMR applications in chemistry, biochemistry, medicinal chemistry and molecular biology, explaining the protocols used and how to interpret the results. With its focus on practical aspects and applications, this text will prove useful long after leaving college, by helping users to select experimental methods and in setting up and running their own NMR experiments.

Annual Reports on NMR Spectroscopy, Volume 100, is a premier resource for both specialists and non-specialists who are looking to become familiar with new techniques and applications in NMR spectroscopy. Chapters in this new release cover In Operando NMR Studies, Recent Developments in Automotive Differential Analysis of NMR Results, Applications of SIMPSON to NMR Studies of Peptides and Proteins, Recent Developments in NMR Line Shape Analysis, and more.

This volume presents a complete and thorough examination of advances in the instrumentation, evaluation, and implementation of UV technology for reliable and efficient data acquisition and analysis. It provides real-world applications in expanding fields such as chemical physics, plasma science, photolithography, laser spectroscopy, astronomy and atmospheric science.

Inelastic neutron scattering (INS) is a spectroscopic technique in which neutrons are used to probe the dynamics of atoms and molecules in solids and liquids. This book is the first, since the late 1960s, to cover the principles and applications of INS as a vibrational-spectroscopic technique. It provides a hands-on account of the use of INS, concentrating on how neutron vibrational spectroscopy can be employed to obtain chemical information on a range of materials that are of interest to chemists, biologists, materials scientists, surface scientists and catalyst researchers. This is an accessible and comprehensive single-volume primary text and reference source.

Raman Spectroscopy in Archaeology and Art History highlights the important contributions Raman spectroscopy makes as a non-destructive method for characterising the chemical composition and structure and in determining the provenance and authenticity of objects of archaeological and historical importance. It brings together studies from diverse areas and represents the first dedicated work on the use of this technique in this increasingly important field. Coverage includes: An Introduction to Raman Spectroscopy, including practical aspects of Raman spectroscopy and complementary techniques; Dyes and Pigments; Artefacts; Biological Materials and Degradation; Jewellery and Precious Stones. The book contains a broad selection of real-world examples in the form of case studies to provide the reader with a true appreciation of the procedures that need to be invoked to derive spectroscopic information from some of the most challenging specimens and systems. Colour illustrations of objects of investigation and a database of 72 Raman spectra of relevant minerals are included. With its extensive examples, Raman Spectroscopy in Archaeology and Art History will be of particular interest to specialists in the field, including researchers and scientific/conservation staff in museums. Academics will find it an invaluable reference to the use of Raman spectroscopy.

The applications of solvent extraction (SX) and liquid membranes (LM) span chemistry, metallurgy, hydrometallurgy, chemical/mineral processing, and waste treatment-making it difficult to find a single resource that encompasses fundamentals as well as advanced applications. Solvent Extraction and Liquid Membranes: Fundamentals and Applications in New Materials draws together a diverse group of internationally recognized experts to highlight key scientific and technological aspects of solvent extraction that are critical to future work in the field. The first chapters identify relevant thermodynamics, kinetics, and interfacial behavior principles and introduce methods for calculating extraction equilibria and kinetic parameters. The next chapters focus on engineering and technological aspects of various industrial processes and plant applications, including optimization and modeling tools and calculations. The final chapters examine new materials for metal extraction and separations, covering preparation and application processes for organic and inorganic sorbents, solid polymeric extractants, and solvent impregnated resins. Solvent Extraction and Liquid Membranes offers a comprehensive review of the most important principles, calculations, and procedures involved in this widely applicable separation technique. The book's pedagogical approach will benefit students and researchers in the field as well as working scientists and engineers who wish to apply solvent extraction to their own applications.

The focus of Volume 2 of the Encyclopedia of Mass Spectrometry is on peptides and proteins, separation techniques, preparation protocols, and the fundamental characteristics as ionic gas phase species that lie within the breadth of the field of mass spectrometry. This volume is divided into four sections; experimental approaches and protocols, sequence analysis, other structural analyses, and targeted applications.
* Frontier Research on biological, biochemical, and biomedical applications of MS
* Focus on Peptides and Proteins
* Suitable for new graduate students entering the field

Mass Spectrometry: A Foundation Course is a textbook covering the field of mass spectrometry across the chemical, physical, biological, medical and environmental sciences. Sufficient depth is provided for the reader to appreciate the reasons behind and basis for particular experiments. It is uniquely and logically organised to enable the book to form the basis for a university course in mass spectrometry at the undergraduate or postgraduate level. This is achieved by combining specific core sections coupled to optional areas of study tailored to students of the chemical, physical, biological, medical and environmental sciences. Recommended course structures are provided in the front of the book.

Dedicated chapters are included on: organic mass spectrometry; ion chemistry - to emphasise the role of mass spectrometry in fundamental chemistry and physics; biological mass spectrometry including proteomics; mass spectrometry in medicine, environmental and surface science and accelerator mass spectrometry, to emphasise the importance of these areas. Each chapter concludes with key references and additional recommended reading material, making the book an excellent springboard to further study.

Highly readable, easy-to-use and logically presented, Mass Spectrometry: A Foundation Course is an ideal text for students and for those who work with mass spectrometers who wish to gain a solid understanding of the basics in modern mass spectrometry.

"From the reviews: "

"

Although I am not a fan or either "eras" or "omes" you hear all the time that we now live in the era of the proteome. Setting aside the issues of what constitutes proteomics (after all people have been sequencingproteins and studying their structure for a few years nowA... ), and whether the regular appearance of reports of another organisms genome sequence prevents you from saying that we are in the post-genomic era, it is clear that the analysis of large numbers of complex protein mixtures is just about in all of our reach. This is going to be a very important way to look for molecular markers and targets in the battle against cancer.

The bedrock of proteomic analysis is mass spectrometry, which allows you to accurately measure the mass of molecules. In proteomics this can mean studying the mass of intact proteins, which can give you a clue as to their identity, and help you rapidly identify modifications. It can also mean busting the protein into many component fragments and measuring their mass, which can lead to protein identification via clever algorithms that compare measured fragment sizes to predicted ones using the genome databases.
One sign that this technology is about to become widely distributed is the appearance of relatively small softcover textbooks for beginners. These represent a reasonable first step towards initiation in this area, for those of us who left school a few years ago. There are two recent ones that I have read that I found to be particularly useful.

The first is Mass Spectrometry: A Foundation Course by Kevin Downard of Sydney, Australia.

This book covers many aspects of the field in under 200 pages, and has a handy guide to what sections are useful to individuals from different disciplines. It starts with history and concepts, and then devotes a significant amount of space to the instrumentation. This is very useful to anyone who has been to a massspectrometry meeting and trade show or even browsed the relevant companies websites. Dr. Downard covers the basics of how each variant works, and what it is best suited for, and includes discussion of single and tandem instruments. By the end you'll be able to raise your eyebrows appreciatively the next time a salesman fires an acronym and figure at you (or at least you'll know where to look it up once you have reached a safe distance). The second half of the book looks at specific applications for mass spectrometry, and here you can read selectively on what you are interested in doing. The sections on protein analysis were good primers. The book is quite mathematical throughout, and since I have no talent in this direction, the equations merely confirmed my deficiency in this area - to those of you who like it, it is there. Then at the end are a series of very useful appendices that show amino acid masses, masses of common protein modifications and websites for further reading or for protein identification, among other useful things. I recommend this book highly to anyone looking for a first port of call on the journey to mass spectrometry." [Oliver Bogler]

"From the reviews: "

"

Mass spectrometry today plays a vital role in a range of scientific disciplines including synthetic and physical organic chemistry, biological and medicinal chemistry and environmental and surface sciences.
In the past two decades, advances in instrument design and in computer control have brought the technique out of the basements of chemical laboratories and onto the bench of modern analytical laboratories. Advances in linking mass spectrometry to both gas and liquid chromatography have meantthat chromatographers have had to learn the extra dimensions of experiment that such multi-dimensional techniques can provide. Mass spectrometry is not now confined to research but is being increasingly used by environmental agencies, clinical chemistry laboratories and anywhere advanced analytical chemistry is required.

Few introductory texts have kept up with these advances, although there have been recent specialist texts on biotechnology and on LC/MS. Downard's book seeks to provide a basis for instruction of undergraduate and new postgraduate students. His philosophy has been to write introductory sections for all the basic aspects of mass spectrometry - his 'core' course - and to add sections for the major areas in which it is employed. These additional sections can be optionally added to the core course, as desired. Downard provides a menu suggesting how they can be put together. Each core section is well written and covers the basis of the science concerned very satisfactorily.

The sections that describe the different applications are necessarily condensed and the author tries to give extra reading with a good set of references.

Students who plan to use mass spectrometry in proteomic research will require much further assistance and the same goes for metabolomic/metabonomic studies and for users of quantitative analysis, but, for both, the core sections will be valuable." [Tony Mallet]

Advances in Chromatography is a venerable series that has reported on the latest state-of-the-art developments in the field for the past four decades. The newest installment, Volume 49, continues the tradition of compiling the work of expert contributors who present timely and cutting edge reviews of current and emerging methods and applications in this dynamic field. Highlights in this edition include: The hyphenation of liquid chromatography with mass spectrometry in order to determine oligonucleotide adducts as markers for cancer Glycoproteomics and the glycosylation of proteins, addressing biomarkers in different types of diseases Chiral separation, an important area particularly in the pharmaceutical industry, where the technique has been applied with varying results Ion-pairing chromatography and analyte retention Conveying the most recent significant scientific developments in separation science, the book and its series are known for the authors' clear presentation of topics and vivid illustrations. Accessible and engaging, this volume forms a solid foundation for the work of biochemists and analytical, organic, polymer, and pharmaceutical chemists at all levels of technical skill. Meticulously referenced, it will help fuel further research across a range of fields.

The first edition of this now classic work helped to establish mass spectrometry as the premier tool for drug metabolism studies. Completely rewritten from start to finish, Using Mass Spectrometry for Drug Metabolism Studies, Second Edition brings medicinal chemists and mass spectrometry professionals up to speed with the rapid advances in the field, the emergence of cutting-edge approaches, and ways to meet steadily increasing vendor demands. Written by international scientists who are experts in their respective disciplines, this state-of-the-art reference effectively encapsulates current mass spectrometry best practices. The stand-alone chapters cover various topics - from metabolite identification to fast chromatography with UPLC - and in a style that is understandable to experts and field newcomers alike. The second edition of this bestseller includes coverage of new instrumentation and software as well as a wealth of updated information on the latest findings surrounding biomarkers and metabolomics and new chapters on both UPLC and DESI/DART. With more than 180 illustrations and an eight-page color insert, this valuable reference explores multiple modern mass spectrometry techniques and strategies. It includes an excellent overview of the entire drug discovery process plus the latest developments on how mass spectrometry is used to support this endeavor.